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11 results for “Multigene analyses”
Fig. 1 in Phylogenetic Analyses on the Tintinnid Ciliates (Protozoa, Ciliophora) Based on Multigene Sequence Data
Fig. 1. Alignment of the ITS1-5.8S-ITS2 regions from ten reference tintinnid species: Tintinnopsis sp. 1, Tintinnopsis sp. 2, Tintinnopsis sp. 3, T. cylindrica, T. tubulosoides, T. lohmanni, Stenosemella nivalis, Codonellopsis nipponica, Favella campanula, F. taraikaensis, F. ehrenbergii, Metacylis angulata, Eutintinnus pectinis, and Amphorellopsis acuta. Agreement with other sequences is indicated by periods and disagreement by a nucleotide at a position. Gaps introduced to improve the alignment are indicated by dashes. The insertion in ITS1 of F. campanula is labeled. The ITS1 and ITS2 region sequences are shaded; the 5.8S gene sequence is unshaded.
Figs 3–5 in Phylogenetic Analyses on the Tintinnid Ciliates (Protozoa, Ciliophora) Based on Multigene Sequence Data
Figs 3–5. Phylogenetic analyses and photomicrographs in this work. 3, 4 – phylogenetic analyses inferred by ML of internal transcribed spacer (ITS) and 5.8S region sequences and small subunit rDNA sequences. Topologies of trees constructed with other methods (BI, MP, or NJ) were essentially identical, lacking only a few nodes indicated by asterisks in the support values. Posterior probability values for branches of the ML tree and bootstrap values for ML, NJ, and MP trees, respectively, are given on nodes. Newly sequenced species are highlighted in bold. Scale bar in 3 corresponds to 10 substitutions per 100 nucleotide positions, scale bar in 4 corresponds to 5 substitutions per 100 nucleotide positions. 5 – photomicrographs of nine of the 10 newly sequenced tintinnid species in vivo: A – Amphorellopsis acuta; B – Favella taraikaensis; C – F. campanula; D – Tintinnopsis sp. 2; E – Stenosemella nivalis; F – Codonellopsis nipponica; G – Tintinnopsis sp. 3; H – T. lohmanni and I – T. cylindrica. Scale bars: 25 μm.
Figure 4 in Revision of the higher taxonomy of Neotropical freshwater crabs of the family Pseudothelphusidae, based on multigene and morphological analyses
Figure 4. Morphological traits of the first male gonopod (G1) of some representative species of Pseudothelphusoidea. A, Epiloboceridae: Epilobocera cubensis Stimpson, 1860 (right G1, mesial view SMF 3880). B–I, Pseudothelphusidae. B, Strengerianinae: Chaceus curimanensis Campos & Valencia, 2004 (left G1, caudalmesial view, INPA 1488). C, Hypolobocerinae: Hypolobocera rathbunae Pretzmann, 1968 (right G1, caudal view, MZUSP 6383). D, Guinotinae: Guinotia dentata Latreille, 1825 (left G1, caudalmesial view, INPA 1445). E, Kingsleyinae: Kingsleya gustavoi Magalhães, 2005 (left G1, caudal view, holotype, INPA 1320, reproduced from Magalhães, 2005). F, Ptychophallinae: Ptychophallus tristani (Rathbun, 1896) (left G 1, caudoalmesial view, holotype, USNM 19047, reproduced from Magalhães et al., 2015). G, Potamocarcininae: Potamocarcinus armatus H. Milne Edwards, 1853 (left G1, mesial view, INPA 2123). H, Raddausinae, Raddaus bocourti (A. Milne-Edwards, 1866) (left G1, mesial view, INPA 2020). I, Pseudothelphusinae: Pseudothelphusa americana de Saussure, 1857 (right G1, mesial view, syntype, MHNGenève uncatalogued). Scale bars = 1 mm.
Figure 1. Bayesian maximum clade credibility tree obtained for 32 in Revision of the higher taxonomy of Neotropical freshwater crabs of the family Pseudothelphusidae, based on multigene and morphological analyses
Figure 1. Bayesian maximum clade credibility tree obtained for 32 genera of the superfamily Pseudothelphusoidea. Values at nodes represent bootstrap values for the Maximum Likelihood analysis (above branches) and posterior probabilities (below branches).
Figure 3 in Revision of the higher taxonomy of Neotropical freshwater crabs of the family Pseudothelphusidae, based on multigene and morphological analyses
Figure 3. Distribution areas of the Pseudothelphusoidea, by families and subfamilies, in the American continent.
FIGURE 3 in Morphological and multigene phylogenetic analyses reveal two new nematodetrapping fungi (Arthrobotrys, Orbiliaceae) from Yunnan, China
FIGURE 3. Arthrobotrys luzhangensis (CGMCC3.20941, holotype!). a Colony. b Microconidia. c Macroconidia. d Chlamydospores. e Trapping-device: adhesive networks. f Microconidiophores. g Macroconidiophores. Bars: a =10 mm; b–g =10 μm.
FIGURE 2 in Morphological and multigene phylogenetic analyses reveal two new nematodetrapping fungi (Arthrobotrys, Orbiliaceae) from Yunnan, China
FIGURE 2. Arthrobotrys gongshanensis (CGMCC3.23753, holotype!). a Colony on PDA. b, c Conidia. d Chlamydospores. e Trapping device: adhesive networks. f Conidiophores. Bars: a =10 mm; b-f =20 μm.
FIGURE 1 in Morphological and multigene phylogenetic analyses reveal two new nematodetrapping fungi (Arthrobotrys, Orbiliaceae) from Yunnan, China
FIGURE 1. Maximum likelihood tree based on the combined ITS, EF1-α and RPB2 sequence data. The new isolates are in blue bold, and ex-type strains are in black bold. Bootstrap support values greater than 70% and Bayesian posterior probabilities values greater than 0.90 are indicated above the nodes. The tree is rooted by Vermispora fusarina YXJ02-13-5.
Fig. 2A–O in Phylogenetic Analyses on the Tintinnid Ciliates (Protozoa, Ciliophora) Based on Multigene Sequence Data
Fig. 2A–O. Secondary structure of the internal transcribed spacer 2 (ITS2) RNA transcript of: A – Strombidinopsis sp.; B – Amphorellopsis acuta; C – Eutintinnus pectinis; D – Stenosemella nivalis; E – Codonellopsis nipponica; F – Tintinnopsis lohmanni; G – T. cylindrica; H – T. tubulosoides; I – Tintinnopsis sp. 2; J – Tintinnopsis sp. 1; K – Favella taraikaensis; L – F. ehrenbergii; M – F. campanula; N – Metacylis angulata and O – Tintinnopsis sp. 3. The diagram illustrates that all these species have a similar ITS2 secondary structure model – one palm with two fingers. Tintinnopsis sp. 1 and Tintinnopsis sp. 3 have the same ITS2 secondary structure, so are shaded together. Positions labeled II that lack a bulge are marked with arrows. Note that a bulge is present in this position in other species.
Data from: Multigene phylogenetic analyses of the Thelonectria coronata and T. veuillotiana species complexes
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Figure 2 in Revision of the higher taxonomy of Neotropical freshwater crabs of the family Pseudothelphusidae, based on multigene and morphological analyses
Figure 2. Tree with divergence estimates for nodes of the main groups of the Pseudothelphusoidea (numbers at the nodes represent millions of years before present), with 95% confidence intervals represented by the grey bars. Three fossil crabs were incorporated into the analysis: (1) Hillius youngi Bishop, 1983, from the lower Cretaceous; (2) Tanzanonautes tuerkai Feldmann et al., 2007 in the Potamonautidae, from the Paleogene of Tanzania; and (3) an undetermined Trichodactylidae from the late Middle Paleocene of Peru (Klaus et al., 2017).
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