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36 results for “NADPH”
Figure 3 in Purification Of Adult Hymenolepis Diminuta (Cestoda) Mitochondrial Nadph Nad Transhydrogenase
Figure 3. Effects of non-immune and immune IgG on the activity of membrane-associated and purified Hymenolepis diminuta transhydrogenase. Preparations and treatments are indicated as follows: Broken line and closed circles, mitochondrial membranes plus non-immune IgG; solid line and open circles, purified enzyme plus non-immune IgG; broken line and closed squares, mitochondrial membranes plus immune IgG; and solid line open squares, plus immune IgG. The activity of untreated mitochondrial membranes and purified enzyme was 0.12 and 8.3 µmol/ min/mg, respectively. Incubations were performed as described in Materials and Methods.
Figure 1 in Purification Of Adult Hymenolepis Diminuta (Cestoda) Mitochondrial Nadph Nad Transhydrogenase
Figure 1. SDS-PAGE of the purification of Hymenolepis diminuta transhydrogenase. Lane designation and amount of protein used were as follows: lane A, mitochondrial membranes, 30 µg; lane B, NaCl-washed membranes, 30 µg; lane C, detergent extract, 25 µg; lane D, DEAE Sepharose I, 5 µg; lane E, DEAE Sepharose II, 5 µg; lane F, hydroxylapatite, 5 µg. Relative positions of protein markers are indicated at the left. Protein was visualized as described in Materials and Methods.
Figure 2 in Purification Of Adult Hymenolepis Diminuta (Cestoda) Mitochondrial Nadph Nad Transhydrogenase
Figure 2. Immunoblot of Hymenolepis diminuta transhydrogenase. Lane designation, amount of protein used, and treatment were as follows: lane A, mitochondrial membranes, 30 µg plus non-immune IgG; lane B, mitochondrial membranes, 30 µg plus immune IgG; lane C, purified enzyme, 5 µg plus non-immune protein, IgG; lane D, purified enzyme, 5 µg plus immune IgG. Relative positions of protein markers are indicated at the left. Immunoblots were performed as described in Materials and Methods.
Fig. 8 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases
Fig. 8. HPLC chromatogram of the mogrol standard and Agrobacterium-mediated transient expression of fruit harbouring pTRV-SgCPRs and pTRV-0 at 24 h after agroinfiltration.
Fig. 9 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases
Fig. 9. (A) Mogrol content in the S. grosvenorii fruit harbouring PBI121, PBI121-SgCPR1, and PBI121-SgCPR2. (B) MIIE content in the S. grosvenorii fruit harbouring PBI121, PBI121-SgCPR1, and PBI121-SgCPR2. (C) MIII content in the S. grosvenorii fruit harbouring PBI121, PBI121-SgCPR1, and PBI121-SgCPR2. (D) Accumulation of mogrol in the S. grosvenorii fruit harbouring pTRV, pTRV-SgCPR1, and pTRV-SgCPR2. (E) Accumulation of MII in the S. grosvenorii fruit harbouring pTRV, pTRV- SgCPR1, and pTRV-SgCPR2. (F) Accumulation of MIII in the S. grosvenorii fruit harbouring pTRV, pTRV-SgCPR1, and pTRV-SgCPR2. The values are the mean ± SD of three independent biological replicates.
Fig. 7 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases
Fig. 7. HPLC chromatogram of the mogrol standard and Agrobacterium-mediated transient expression of fruit harbouring PBI121-SgCPRs and PBI121 at 24 h after agroinfiltration.
Fig. 2 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases
Fig. 2. (A) Cloning of full-length and specific fragments of the SgCPR1 and SgCPR2 genes from S. grosvenorii cDNA. M, marker (2000 bp). The fulllength SgCPR1 and SgCPR2 genes are shown in red brackets, whereas the blue arrow indicates the specific fragments of the SgCPR1 and SgCPR2 genes. (B) Vector map of PBI121. (C) Construction of PBI12- SgCPR recombinant plasmids with the GUS marker and CaMV35S promoter. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fig. 5 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases
Fig. 5. qRT-PCR analysis of SgCPR1 and SgCPR2 in fruit after infiltration (at t = 0 h). Relative expression levels were normalized to t = 0 h. (A) Relative transcript levels of SgCPR1 in fruit harbouring PBI121- SgCPR1 and PBI121. (B) SgCPR2 gene expression levels in fruit harbouring PBI121 and PBI121-SgCPR2. (C) Relative expression levels of SgCPR1 in fruit harbouring pTRV-SgCPR1 and pTRV-0. (D) Expression levels of SgCPR2 in fruit harbouring pTRV-SgCPR2 and pTRV-0. The relative expression levels normalized to the level of the SgUBQ gene. All the data are shown as means±SDs. * indicates significant differences at p <0.05 (LSD test).
Fig. 3 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases
Fig. 3. (A) Selective nucleotide sequences for VIGS in S. grosvenorii, which were inserted into EcoRI and BamHI. (B) pTRV2-SgCPR recombinant plasmid construction.
Fig. 1 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases
Fig. 1. The mogroside biosynthetic pathway in S. grosvenorii. The blue box on the left shows the generation of mogrol, which is a unique nonglycosylated tetra-hydroxycucurbitane in S. grosvenorii. The red box on the right represents a series of glycosylation reactions. The enzyme names are abbreviated as follows: SgSQE, squalene epoxidase; SgCS, cucurbitadienol synthase; SgEPH, epoxide hydrolase; SgCYP450, cytochrome P450 monooxygenase; SgCPR, NADPHcytochrome P450 reductase; SgUGT, UDP-glucosyltransferase. Among these, the candidate SgCPRs are highlighted in red. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fig. 6 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases
Fig. 6. Accumulation of MIII and MIIE. (A) Total ion chromatograms of ten standard mixtures determined by LC-MS/MS. Among these, the retention times of MIII and MIIE were 10.01 and 12.09 min, respectively. (B) Extracted ion chromatograms of the sample. The red arrow indicates the Q1/Q3 mass chromatograms of MIII, which are in the red box. The Q1/Q3 mass chromatograms of MIIE are shown in the blue box, as indicated by the blue arrow. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
Fig. 4 in Development of an efficient transient expression system for Siraitia grosvenorii fruit and functional characterization of two NADPH-cytochrome P450 reductases
Fig. 4. Histochemical GUS assay of transient expression in S. grosvenorii. GUS expression was detected in fruit but not in CK or leaves.
NADPH Oxidase Correction in mRNA-transfected Granulocyte-enriched Cells in Chronic Granulomatous Disease (CGD)
ClinicalTrials.gov study NCT05189925. IPD Sharing: Not stated. Countries: 1. Publications: 3.
NADPH oxidase 5 has a crucial role in cellular motility of colon cancer cells
GEO Series GSE175691. Homo sapiens. 1 samples. Type: Expression profiling by array.
The role of phagocyte NADPH Oxidase (NOX2) in modulating the antimicrobial and inflammatory functions of mouse neutrophils
GEO Series GSE256399. Mus musculus. 12 samples. Type: Expression profiling by high throughput sequencing.
The leukocyte NADPH oxidase 2 (NOX2) plays a key role in pathogen killing and immunoregulation
GEO Series GSE198778. Mus musculus. 42 samples. Type: Expression profiling by array; Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing.
Cytosolic Accumulation of Small Nucleolar RNAs (snoRNAs) is Dynamically Regulated by NADPH Oxidase
GEO Series GSE67050. Rattus norvegicus. 12 samples. Type: Non-coding RNA profiling by high throughput sequencing.
NADPH oxidase 4 (Nox4) deletion accelerates liver regeneration in mice
GEO Series GSE181476. Mus musculus. 37 samples. Type: Expression profiling by high throughput sequencing.
The reducing equivalent NADPH dictates histone acetylation via direct inactivation of HDAC3
GEO Series GSE137694. Mus musculus. 9 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Phagocytosis-related NADPH oxidase 2 subunit gp91phox contributes to neurodegeneration after repeated systemic challenge with lipopolysaccharides
GEO Series GSE153369. Mus musculus. 24 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
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