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117 results for “Oligosaccharides”
Three-Enzyme Phosphorylase Cascade Immobilized on Solid Support for Biocatalytic Synthesis of Cello-oligosaccharides
<p>We provide here the underlying data of the scientific publication "Three-Enzyme Phosphorylase Cascade Immobilized on Solid Support for Biocatalytic Synthesis of Cello-oligosaccharides". Please find the abstract below:</p> <p>Enzyme cascades are promising for multistep biocatalytic synthesis, but their effective use beyond the proof-of-concept stage is challenging. Strategies to recycle the individual enzymes are critical for the applicability of such cascades. Immobilization on solid support is well developed for single enzymes but remains difficult for enzyme ensembles. Here, we show a controlled co-immobilization of three glycoside phosphorylases to establish a highly active and recyclable biocatalyst for the conversion of sucrose and glucose into soluble (short-chain) cello-oligosaccharides. We use protein fusion with the binding module Z<sub>basic2 </sub>to enable non-covalent surface tethering of all enzymes according to a uniform principle and in a programmable fashion. We thus achieve loading of the phosphorylases in an activity ratio optimal for the overall conversion rate and for controlling the cello-oligosaccharide chain length (≤ 6), hence the solubility, in the reaction. We demonstrate efficient production of ~12 g/L cello-oligosaccharides in 5 reaction cycles with integrated enzyme re-use. This study presents a major advance toward the practical use of systems bio-catalysis on solid support.</p>
Kinetic modeling of phosphorylase-catalyzed iterative β-1,4-glycosylation for degree of polymerization-controlled synthesis of soluble cello-oligosaccharides
<p>We provide here the underlying data of the publication "Kinetic modeling of phosphorylase-catalyzed iterative β-1,4-glycosylation for degree of polymerization-controlled synthesis of soluble cello-oligosaccharides". Please find the abstract below.</p> <p><strong>Background: </strong>Cellodextrin phosphorylase (CdP; EC 2.4.1.49) catalyzes the iterative β-1,4-glycosylation of cellobiose using α-D-glucose 1-phosphate as the donor substrate. Cello-oligosaccharides (COS) with a degree of polymerization (DP) of up to 6 are soluble while those of larger DP self-assemble into solid cellulose material. The soluble COS have attracted considerable attention for their use as dietary fibers that offer a selective prebiotic function. An efficient synthesis of soluble COS requires good control over the DP of the products formed. A mathematical model of the iterative enzymatic glycosylation would be important to facilitate target-oriented process development.<br> <strong>Results: </strong>A detailed time-course analysis of the formation of COS products from cellobiose (25 mM, 50 mM) and α-D-glucose 1-phosphate (10–100 mM) was performed using the CdP from <em>Clostridium cellulosi</em>. A mechanism-based, Michaelis–Menten type mathematical model was developed to describe the kinetics of the iterative enzymatic glycosylation of cellobiose. The mechanistic model was combined with an empirical description of the DP-dependent self-assembly of the COS into insoluble cellulose. The hybrid model thus obtained was used for kinetic parameter determination from time-course fits performed with constraints derived from initial rate data. The fitted hybrid model provided excellent description of the experimental dynamics of the COS in the DP range 3–6 and also accounted for the insoluble product formation. The hybrid model was suitable to disentangle the complex relationship between the process conditions used (i.e., substrate concentration, donor/acceptor ratio, reaction time) and the reaction output obtained (i.e., yield and composition of soluble COS). Model application to a window-of-operation analysis for the synthesis of soluble COS was demonstrated on the example of a COS mixture enriched in DP 4.<br> <strong>Conclusions:</strong> The hybrid model of CdP-catalyzed iterative glycosylation is an important engineering tool to study and optimize the biocatalytic synthesis of soluble COS. The kinetic modeling approach used here can be of a general interest to be applied to other iteratively catalyzed enzymatic reactions of synthetic importance.</p>
Evaluating the Efficacy of the Mediterranean Diet to the Low- Fermentable, Oligosaccharides, Disaccharides, Monosaccharides, and Polyols (FODMAP) Diet in Treating Irritable Bowel Syndrome(IBS)
ClinicalTrials.gov study NCT05807919. IPD Sharing: NO. Countries: 1. Publications: 1.
Short-chain cello-oligosaccharides: Intensification and scale-up of their enzymatic production and selective growth promotion among probiotic bacteria
<p>We provide here the underlying data of the scientific publication "Short-chain cello-oligosaccharides: Intensification and scale-up of their enzymatic production and selective growth promotion among probiotic bacteria". Please find the abstract below:</p> <p>Short-chain cello-oligosaccharides (COS; degree of polymerization, DP≤6) are promising water-soluble dietary fibers. An efficient approach to their bottom-up synthesis is from sucrose and glucose using glycoside phosphorylases. Here, we show intensification and scale up (20 mL; gram scale) of COS production to 93 g/L product and in 82 mol % yield from sucrose (0.5 M). The COS were comprised of DP 3 (33 wt %), DP 4 (34 wt %), DP 5 (24 wt %) and DP 6 (9 wt %) and involved minimum loss (≤10 mol %) to insoluble fractions. After isolation (≥95% purity; ≥90% yield), the COS were examined for growth promotion of probiotic strains. Benchmarked against inulin, trans-galacto-oligosaccharides and cellobiose, COS showed up to 4.1-fold stimulation of cell density for <em>Clostridium butyricum</em>, <em>Lactococcus lactis</em> subsp.<em>lactis</em>, <em>Lactobacillus paracasei</em> subsp. <em>paracasei</em> and <em>Lb. rhamnosus</em> but were less efficient with <em>Bifidobacterium</em> sp. This study shows the COS as selectively functional carbohydrates with prebiotic potential and demonstrates their efficient enzymatic production.</p>
Targeting host-virus interactions: In silico analysis of the binding of human milk oligosaccharides to viral proteins involved in respiratory infections
<p><span>Respiratory viral infections, a major public health concern, necessitate the continuous development of novel antiviral strategies, particularly in the face of emerging and re-emerging pathogens. In this study, we </span><span>explored</span><span> the potential of human milk oligosaccharides (HMOs) as broad-spectrum antiviral agents against key respiratory viruses. </span><span>By examining the</span><span> structural mimicry of host cell receptors and </span><span>their </span><span>known biological functions, including antiviral activities, we assessed the </span><span>ability</span><span> of HMOs to bind and potentially inhibit viral proteins crucial for host</span><span> </span><span>cell entry. Our <em>in silico</em> analysis </span><span>focused</span><span> on viral proteins integral to host-virus interactions</span><span>, namely,</span><span> the hemagglutinin protein of influenza, fusion proteins of respiratory syncytial and human metapneumovirus, and the spike protein of SARS-CoV-2. Using molecular docking and simulation studies, we </span><span>demonstrated</span><span> that HMOs exhibit varying binding affinities to these viral proteins, suggesting their potential as viral entry inhibitors. </span><span>This</span><span> study </span><span>identified</span><span> several HMOs with promising binding profiles, highlighting their potential in antiviral drug development. This research provides a foundation for utilizing HMOs as a natural source for designing new therapeutics, offering a novel approach in the fight against respiratory viral infections.</span></p>
Effect of Physical Activity in Pregnancy on Maternal and Fetal Human Milk Oligosaccharides: a Pilot Study
ClinicalTrials.gov study NCT05496712. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Impact of Galacto-Oligosaccharides on Adult Microbiome
ClinicalTrials.gov study NCT05207839. IPD Sharing: NO. Countries: 1. Publications: 1.
Iron Absorption From an Iron-fortified Follow-up Formula With Added Synbiotic or Human Milk Oligosaccharides
ClinicalTrials.gov study NCT04774016. IPD Sharing: UNDECIDED. Countries: 1. Publications: 1.
Tolerance and Prebiotic Activity of Arabinoxylan-Oligosaccharides in Healthy Subjects
ClinicalTrials.gov study NCT00852813. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Exploring the Impact of Perioperative Galacto-Oligosaccharides (GOS) on Stress, Anxiety and Cognition
ClinicalTrials.gov study NCT02953691. IPD Sharing: NO. Countries: 1. Publications: 2.
Safety and Efficacy of an Infant Formula Supplemented With Galacto-oligosaccharides, Beta-palmitate and Acidified Milk
ClinicalTrials.gov study NCT01197365. IPD Sharing: Not stated. Countries: 1. Publications: 25.
The Role of Human Milk Oligosaccharides and Microbiomes on Infantile Colic and Atopic Dermatitis in Term Infants
ClinicalTrials.gov study NCT05992493. IPD Sharing: UNDECIDED. Countries: 1. Publications: 3.
Effects of Arabinoxylan-Oligosaccharides Compared to Other Prebiotics in Healthy Subjects
ClinicalTrials.gov study NCT00853034. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Safety Study of Human Milk Oligosaccharides in Healthy Adults
ClinicalTrials.gov study NCT05516225. IPD Sharing: Not stated. Countries: 1. Publications: 1.
In Vivo Evaluation of the Physiological Effects of Arabinoxylan-oligosaccharides (AXOS) in Healthy Subjects (Juniors)
ClinicalTrials.gov study NCT01001949. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Human Milk Oligosaccharides (HMOs) Post-market Study on Infants (NEHMO)
ClinicalTrials.gov study NCT05150288. IPD Sharing: Not stated. Countries: 3. Publications: 1.
Arabinoxylan-oligosaccharides (AXOS) for the Management of Type-2 Diabetes
ClinicalTrials.gov study NCT05689684. IPD Sharing: NO. Countries: 1. Publications: 5.
Second Generation Human Milk Oligosaccharides Blend Study
ClinicalTrials.gov study NCT03722550. IPD Sharing: NO. Countries: 3. Publications: 1.
Human Milk Oligosaccharides (HMOs) - Post Marketing Study on Infants (NeHMO)
ClinicalTrials.gov study NCT07302477. IPD Sharing: NO. Countries: 1. Publications: 1.
Human Milk Oligosaccharides (HMOs) Post-market Study on Infants
ClinicalTrials.gov study NCT04055363. IPD Sharing: Not stated. Countries: 1. Publications: 1.
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