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Fig. 9 in New species of Pavlovophyceae (Haptophyta) and revision of the genera Exanthemachrysis, Rebecca and Pavlova

Fig. 9. Rebecca billardiae Véron sp. nov. (AC537), flagellar apparatus details of motile cells. A–E. SEM images. A. Ventral view of a cuboid cell with the S-shaped distally tapered AF and curved H. B-C. Ventral views of angular cells with the S-shaped AF covered with KS, the vestigial PF and the curved bipartite H between both F. D-E. Details of the insertion the 3 appendages. D. Vestigial PF. E. Bipartite H with the proximal hook-shaped half and the distal half forming a pearl string structure. Note distally tapered AF. F–G. Negative staining images. F. Proximal part of the tomentose AF with multilayered KS (white arrow), extending after a bare proximal zone (black arrow) in the immediate vicinity of the cell. G. Details of mono-constricted (white arrows) KS covering the distally attenuated AF. Scale bars: A, C, E–F = 1 µm; B = 2 µm; D = 100 nm; G = 200 nm.

Fig. 8 in New species of Pavlovophyceae (Haptophyta) and revision of the genera Exanthemachrysis, Rebecca and Pavlova

Fig. 8. Rebecca billardiae Véron sp. nov. (AC537), flagella, plasma membrane and knob-scales, TEM images (A–D, G–H = Fix 1; E–F = Fix 2). A. Angular cell with bilobed C and TH lamellae grouped by 3 sometimes twisted. B. Longitudinal section of a motile cell displaying the AF bearing few KS (white arrows) and the adjacent H. C. Cross-section of the sub-apical pit of a non-swimming cell containing the bases of H and F (transverse section – right, oblique section – left) sheathed by cytoplasm and surrounded by the plasma membrane (black arrow). D. Inflated G secretory vesicles unloading their materials including KS near the flagellar insertion zone. E. Periphery of a cell showing continuity of the plasma membrane along the curved AF, both covered with pedunculate KS (white arrows). F. Isolated plasma membrane retaining pedunculate mono-constricted club-shaped KS sporadically dispersed on its surface. G. Longitudinal section of a motile cell displaying the AF and PF both bearing few KS (white arrows) and the naked H. Note discharge of KS by G cisternae. H. Reduced PF cut in median longitudinal section showing microtubules and bearing a dense pedunculate club-shaped KS (white arrow). Central pair of microtubules is absent. Scale bars: A = 2 µm; B–D, H = 0.5 µm; E–F = 50 nm; G = 1 µm.

Fig. 5 in New species of Pavlovophyceae (Haptophyta) and revision of the genera Exanthemachrysis, Rebecca and Pavlova

Fig. 5. Exanthemachrysis fresneliae Véron sp. nov. (AC35), flagella, plasma membrane and knob-scales, TEM images (Fix 1). A. Longitudinal section of the pit area with 2 emerging F (white arrows = central microtubules) surrounded by a sheath of reticulum enclosing their bases. Plasma membrane (black arrow), underlined with endoplasmic reticulum (white stars), covered with KS. B. Details of the cell surface in the region of F emergence. Oblique sections of F bases with central microtubules (white arrow). Stalked ovoid KS partially covering the F bases and the plasma membrane. C. G with cisternae and secretory vesicles containing KS (white arrows). Scale bars: A–B = 50 nm; C = 0.5 µm.

Fig. 2 in New species of Pavlovophyceae (Haptophyta) and revision of the genera Exanthemachrysis, Rebecca and Pavlova

Fig. 2. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid sister cells, TEM image (Fix 1). Benthic colony of non-motile sister cells embedded in a common non-stratified M envelope (white stars); parietal C with helicoidal arrangement of the TH lamellae and a bulging PY near the F bases perpendicular to each other (white arrows), with the basal bodies connecting to the microtubular root (black arrows) running to the central area of the cell. Secretory vesicles from G (black star) ready to discharge content near the pit where the F emerge. Scale bar: 0.5 µm.

Fig. 12 in New species of Pavlovophyceae (Haptophyta) and revision of the genera Exanthemachrysis, Rebecca and Pavlova

Fig. 12. Pavlova sp. AC248, flagellar apparatus details of motile cells. A. SEM image of apical view of a swimming cell with a complete F apparatus emerging almost in the centre of a narrow, shallow pit: tomentose S-shaped AF, end-tapered PF and bipartite H inserted between both. Bulging PY on the opposite side. B–E. Negative staining images. B. Details of the base of the F apparatus showing the AF, PF with a tapered end, and H consisting of a proximal part of constant diameter and a distal part of subequal length and smaller diameter. C. Distal rounded part of the AF (with a blistering of the membrane) showing covering of long non-tubular hairs and regular arrangement of KS. D. Central part of the AF showing flat KS with a slight median constriction (black arrows) arranged in several more or less regular layers. E. Proximal part of the AF with multilayered KS, extending after a bare area in the immediate vicinity of the cell. Scale bars: A–B = 1 µm; C = 0.5 µm; D = 50 nm; E = 200 nm.

Fig. 4 in New species of Pavlovophyceae (Haptophyta) and revision of the genera Exanthemachrysis, Rebecca and Pavlova

Fig. 4. Exanthemachrysis fresneliae Véron sp. nov. (AC35), flagellar apparatus of motile cells. A. SEM image of side view of swimming cell with a 3–4 µm long conspicuous sinusoidal S-shaped and slightly tapering AF emerging almost mid-ventrally and thickly covered by KS (white arrows). B–C. Negative staining image. B. Detail of the 3 appendices, the AF, the H and the PF composed of 2 parts of sub-equal lengths with a distal attenuation. C. Detail of the AF of a cell bearing ovoid KS (black arrows) down to the base, in regular helicoidal rows. Scale bars: A–B = 1 µm; C = 0.1 µm.

Fig. 1 in New species of Pavlovophyceae (Haptophyta) and revision of the genera Exanthemachrysis, Rebecca and Pavlova

Fig. 1. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid and swimming cells, LM images. A. Benthic colonies of non-motile sister cells embedded in non-stratified M (black arrows) with storage granules of P (white arrows). B. Benthic non-motile cell embedded in non-stratified M (black arrow) with storage granule of P (white arrow), bilobed C and anterior bulging PY. C. Apical view (bottom right) and side view (up left) of 2 swimming cells with conspicuous S-shaped AF (black arrows) emerging almost mid-ventrally from a pit (white arrows). D. Side view of motile cells with bulging PY (black arrows). Scale bars: A = 5 µm; B = 1 µm; C–D = 2 µm.

Fig. 7 in New species of Pavlovophyceae (Haptophyta) and revision of the genera Exanthemachrysis, Rebecca and Pavlova

Fig. 7. Rebecca billardiae Véron sp. nov. (AC537), flagellate motile and non-motile cells, LM images. A. Gathering angular non-swimming flagellate cells with an S-shaped AF (white arrows). B. Polyhedral free-swimming cell with bilobed lateral golden-brown C, S-shaped AF (white arrow) and posterior beaded filipodium (black arrows). C–D. Cells of various shapes showing sub-apical insertion into a pit (white arrows) of the S-shaped AF. Posterior branched beaded filipodia present at the terminal part of the cell (black arrows). E. Ventral view of an angular free-swimming cell with sub-apical insertion of S-shaped AF into a pit (white arrow). F. Rounded cells with S-shaped sub-apically inserted AF (white arrow), posterior filipodium (black arrow) and bilobed golden-brown C. Scale bars: Scale bars: A = 4 μm; B–D, F = 5 μm; E = 1 μm.

Fig. 3 in New species of Pavlovophyceae (Haptophyta) and revision of the genera Exanthemachrysis, Rebecca and Pavlova

Fig. 3. Exanthemachrysis fresneliae Véron sp. nov. (AC35), palmelloid cell, TEM image (Fix 1). Nonmotile benthic cell embedded in non-stratified M showing single parietal C with a bulging PY at its end. Row of few osmiophilic vesicles forming an E in C stroma located at the junction of TH and PY. Both F bases (white arrows) positioned in continuity with a notch in the nucleus facing the sub-ventral pit (black arrow). Scale bar:1 µm.

Fig. 6 in New species of Pavlovophyceae (Haptophyta) and revision of the genera Exanthemachrysis, Rebecca and Pavlova

Fig. 6. Exanthemachrysis fresneliae Véron sp. nov. (AC35), viroplasm, TEM images (Fix 1). A. Benthic non-motile cell embedded in M; parietal C lobes with helicoidal arrangement of the TH lamellae. Cytoplasmic assembly of pentahedral virus (V). B. Viral particles scattered in the cytoplasm. Scale bars: A = 0.2 µm; B = 50 nm.

Fig. 11 in New species of Pavlovophyceae (Haptophyta) and revision of the genera Exanthemachrysis, Rebecca and Pavlova

Fig. 11. Pavlova sp. AC248, chloroplast details, TEM images (Fix 1). A. Benthic colony of non-motile cells surrounded by a single, loose, non-layered M (white stars); single cup-shaped parietal C. B. Longitudinal section of a flagellate cell with parallel arrangement of TH in the C. Posterior PY protruding from the centre of the C, opposite a F, with an E at the tip of the C on its inner face. C. Detail of parallel TH lamellae forming stacks of 4 or 5 ending at the beginning of the PY.D. Detail of F insertion area showing proximity of the intrachloroplastic E to the long flagellar root (white arrows). Scale bars: A–B = 2 µm; C–D = 200 nm.

Fig. 10 in New species of Pavlovophyceae (Haptophyta) and revision of the genera Exanthemachrysis, Rebecca and Pavlova

Fig. 10. Pavlova sp. AC248, motile cells, LM images. Side views of curved oblong (A), ovoid (B) and spherical (C) free-swimming cells with conspicuous S-shaped AF emerging apically. Greenish brown parietal C (black arrows) with an E and a posterior bulging PY. Vacuolar crystals of barium sulphate (white arrows). Scale bars = 10 µm.

opencc-byDec 2016View details →

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Christmas Pavlova

Visual SfM 61 iPhone 7 Photos little to no post processing apart from polygon reduction. Source: Objaverse 1.0 / Sketchfab

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The genome of the haptophyte Diacronema lutheri (Pavlova lutheri, Pavlovales)

Haptophytes are biogeochemically and industrially important protists, but we know little about their genomic diversity. Here we sequenced the nuclear genome of Diacronema lutheri (Pavlova lutheri, Pavlovales) using third generation PacBio long-read sequencing coupled with Illumina sequencing. The genome was assembled with CANU and subsequently polished using both PacBio and Illumina reads to high consensus accuracy. Genome annotation was performed using BRAKER2 and three sets of RNA-seq evidence. The dataset presented here comprises two files comprising (1) the full genome sequence and (2) the annotated protein sequences, each in .fasta format.The genome assembly size is 43.5 Mb and is characterized by a high GC content. Due to the use of long sequencing reads, the genome is highly contiguous and contained in 103 contigs. A total of 14,446 protein-coding genes were annotated, and further details of the assembly and annotation process are available in the publication.

opencc-zeroDec 2020View details →

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The genome of the haptophyte Diacronema lutheri (Pavlova lutheri, Pavlovales)

Open the record for dataset details and reuse information.

publicAug 2021View details →

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Supplementary material 1 from: Bannikova AA, Jenkins PD, Solovyeva EN, Pavlova SV, Demidova TB, Simanovsky SA, Sheftel BI, Lebedev VS, Fang Y, Dalen L, Abramov AV (2019) Who are you, Griselda? A replacement name for a new genus of the Asiatic short-tailed shrews (Mammalia, Eulipotyphla, Soricidae): molecular and morphological analyses with the discussion of tribal affinities. ZooKeys 888: 133-158. https://doi.org/10.3897/zookeys.888.37982

: Explanation note: Figure S1. The phylogenetic relationships in Blarinella as reconstructed in MrBayes based on the extended alignment of cytb. Figure S2. The phylogenetic relationships in Blarinella as reconstructed in MrBayes based on the alignment of ApoB. Table S1. GenBank accession numbers of sequences retrieved from GenBank and newly collected sequences used in the study (marked in bold). Table S2. Primers for cytb amplification and sequencing. Table S3. The best-fit substitution models employed for each of the five partitions found by IQTREE.

opencc-zeroNov 2019View details →

Figure 6 from: Bannikova AA, Jenkins PD, Solovyeva EN, Pavlova SV, Demidova TB, Simanovsky SA, Sheftel BI, Lebedev VS, Fang Y, Dalen L, Abramov AV (2019) Who are you, Griselda? A replacement name for a new genus of the Asiatic short-tailed shrews (Mammalia, Eulipotyphla, Soricidae): molecular and morphological analyses with the discussion of tribal affinities. ZooKeys 888: 133-158. https://doi.org/10.3897/zookeys.888.37982

Figure 6 Comparison of lingual view of posterior region of right mandible to show variation in mandibular and ramal foramina. Mandibular foramen: horizontal arrow; ramal foramen: vertical arrow. From left to right: holotype of Parablarinella griseldaNHMUK 1912.8.5.23; Blarinella quadraticaudaNHMUK 1911.2.1.59; holotype of Blarinella wardiNHMUK 1915.2.3. Scale bar: 1 mm.

Figure 7 from: Bannikova AA, Jenkins PD, Solovyeva EN, Pavlova SV, Demidova TB, Simanovsky SA, Sheftel BI, Lebedev VS, Fang Y, Dalen L, Abramov AV (2019) Who are you, Griselda? A replacement name for a new genus of the Asiatic short-tailed shrews (Mammalia, Eulipotyphla, Soricidae): molecular and morphological analyses with the discussion of tribal affinities. ZooKeys 888: 133-158. https://doi.org/10.3897/zookeys.888.37982

Figure 7 The female karyotype of Blarinella quadraticauda (G17-12) with 2n = 49, NFa = 62: routine Giemsa staining (A) and CBG-banding (B).

Figure 5 from: Bannikova AA, Jenkins PD, Solovyeva EN, Pavlova SV, Demidova TB, Simanovsky SA, Sheftel BI, Lebedev VS, Fang Y, Dalen L, Abramov AV (2019) Who are you, Griselda? A replacement name for a new genus of the Asiatic short-tailed shrews (Mammalia, Eulipotyphla, Soricidae): molecular and morphological analyses with the discussion of tribal affinities. ZooKeys 888: 133-158. https://doi.org/10.3897/zookeys.888.37982

Figure 5 Variation in morphology of the talonid of the lower molars A lingual view of left mandibular ramus of Parablarinella griseldaZMMU G18-252 B lingual view of left mandibular ramus of Blarinella quadraticaudaZMMU G17-12 C lingual view of right mandibular ramus of holotype of Blarinella wardiNHMUK 1915.2.1.3 D comparison of right lower molars to show variation in development of the entoconid and entoconid crest on m1 and m2 and the talonid of m3. Above holotype of Parablarinella griseldaNHMUK 1912.8.5.23, middle Blarinella quadraticaudaNHMUK 1911.2.1.57, below holotype of Blarinella wardiNHMUK 1915.2.1.3. The arrows indicate the entoconid and entoconid crest on m2. Scale bar: 1 mm.

Figure 4 from: Bannikova AA, Jenkins PD, Solovyeva EN, Pavlova SV, Demidova TB, Simanovsky SA, Sheftel BI, Lebedev VS, Fang Y, Dalen L, Abramov AV (2019) Who are you, Griselda? A replacement name for a new genus of the Asiatic short-tailed shrews (Mammalia, Eulipotyphla, Soricidae): molecular and morphological analyses with the discussion of tribal affinities. ZooKeys 888: 133-158. https://doi.org/10.3897/zookeys.888.37982

Figure 4 Skulls from left to right of the holotype of Parablarinella griseldaNHMUK 1912.8.5.23; Blarinella quadraticaudaNHMUK 1911.2.1.59; the holotype of Blarinella wardiNHMUK 1915.2.1.3 (please note that the number written incorrectly as 12.2.1.3 on the skull of this species should read 15.2.1.3). Top row: dorsal view; middle row: ventral view; lower row: left lateral view.