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12 results for “Pelodytidae”

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FIGURE 9. a–c in Integration of molecular, bioacoustical and morphological data reveals two new cryptic species of Pelodytes (Anura, Pelodytidae) from the Iberian Peninsula

FIGURE 9. a–c, Mouthparts of preserved tadpoles of three species of Pelodytes. Note the absence of a central gap in the third anterior labial tooth row in P. ibericus. d, mouthpart, and e, lateral view of a living tadpole of P. ibericus from near Jerez de la Frontera.

opennotspecifiedDec 2017View details →
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FIGURE 6 in Integration of molecular, bioacoustical and morphological data reveals two new cryptic species of Pelodytes (Anura, Pelodytidae) from the Iberian Peninsula

FIGURE 6. Morphometric differentiation among the four western Pelodytes lineages. The upper graph is a scatterplot of first and second principal components from a PCA of raw morphometric values in 117 male specimens of Pelodytes. Note that PC1 corresponds mostly to body size (Table 5). The lower graphs are boxplots of SVL, relative tympanum diameter and relative hindlimb length in male Pelodytes, with median (dot), 25% percentiles (box) and ranges, illustrating the smaller body size of P. atlanticus sp. nov. and P. ibericus, and larger tympanum and shorter limbs of P. ibericus. All specimens of P. ibericus, P. atlanticus, and P. hespericus were measured by JDR, and specimens of P. punctatus were measured by MV.

opennotspecifiedDec 2017View details →
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FIGURE 4 in Integration of molecular, bioacoustical and morphological data reveals two new cryptic species of Pelodytes (Anura, Pelodytidae) from the Iberian Peninsula

FIGURE 4. Species tree calculated from the analysis of DNA sequences of two mitochondrial and six nuclear gene segments (left), after exclusion of admixed/hybrid samples identified from the nDNA networks in Fig. 2 and Bayesian assignment analyses in Fig. 3. The tree on the right shows the same topology as recovered in a species tree analysis based only on the six nuclear gene segments.

opennotspecifiedDec 2017View details →
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FIGURE 1 in Integration of molecular, bioacoustical and morphological data reveals two new cryptic species of Pelodytes (Anura, Pelodytidae) from the Iberian Peninsula

FIGURE 1. Location of 45 populations of Pelodytes sampled for molecular analysis (indicative map unscaled). Colored dots represent the four species described: green, lineage A (P. atlanticus sp. nov.); orange, lineage B (P. ibericus); blue, lineage C (P. hespericus sp. nov.); and purple, lineage D (P. punctatus). In some populations (7, 8, 9, 10, 11, 22, 23, 36 and 37) we found evidence for admixture (double-colored dots). Background colors represent the ranges of P. punctatus (blue) and P. ibericus (orange) according to current knowledge (www.iucn.org).

opennotspecifiedDec 2017View details →
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FIGURE 12 in Integration of molecular, bioacoustical and morphological data reveals two new cryptic species of Pelodytes (Anura, Pelodytidae) from the Iberian Peninsula

FIGURE 12. Dorsal, ventral and dorsolateral view, and close up of ventral side of forelimb, of living Pelodytes hespericus sp. nov.: (a-b) male holotype JDR 2012-1, (c-f); male paratype JDR 2012-2. Not to scale.

opennotspecifiedDec 2017View details →
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FIGURE 5 in Integration of molecular, bioacoustical and morphological data reveals two new cryptic species of Pelodytes (Anura, Pelodytidae) from the Iberian Peninsula

FIGURE 5. Ventral views of specimen hands (palmar surface) of the four western Pelodytes lineages. The pictures show variation in the size and arrangement of the subarticular and metacarpal tubercles within species and populations, suggesting that no fully diagnostic interspecific differences in this character exist. All photos from specimens in life. M, male, F, female. Asterisks denote mirrored images. Not to scale.

opennotspecifiedDec 2017View details →
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FIGURE 3 in Integration of molecular, bioacoustical and morphological data reveals two new cryptic species of Pelodytes (Anura, Pelodytidae) from the Iberian Peninsula

FIGURE 3. Clustering results of Bayesian assignment analyses using Structure, based on nuclear DNA sequences with the number of clusters K ranging from 1 to 7. Each individual is represented as a vertical bar partitioned into K colored segments, whose length is proportional to the individual's estimated group membership coefficient. The enlarged figure shows the results for K=4, with population numbers as in Fig. 1 and Supplementary Table 1. Population numbers on the top are shown to highlight individuals with mixed ancestry, and the mitochondrial haplogroup (A-D) for each included individual is shown in the bottom. Individuals in the graph are ordered as in Supplementary Table S1. Note that the comparatively large number of putative hybrids is due to the overrepresentation of populations in the contact zones in our analysis, as compared with Díaz- Rodríguez et al. (2015).

opennotspecifiedDec 2017View details →
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FIGURE 11 in Integration of molecular, bioacoustical and morphological data reveals two new cryptic species of Pelodytes (Anura, Pelodytidae) from the Iberian Peninsula

FIGURE 11. (a,b) Dorsal and ventral views of preserved Pelodytes atlanticus sp. nov. holotype (male; specimen GLA-03). (c,d) Dorsolateral view of paratype (GLA-02) and holotype (GLA-03) in life. (e-i) dorsolateral, dorsal, and ventral view, as well as ventral view of hand and foot of male paratype ZSM 194/2016. Not to scale.

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FIGURE 10 in Integration of molecular, bioacoustical and morphological data reveals two new cryptic species of Pelodytes (Anura, Pelodytidae) from the Iberian Peninsula

FIGURE 10. Comparative photos of Pelodytes ibericus in life, male above, female below, from near Jerez de la Frontera, Spain. Specimens not collected. Images not to scale.

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FIGURE 8 in Integration of molecular, bioacoustical and morphological data reveals two new cryptic species of Pelodytes (Anura, Pelodytidae) from the Iberian Peninsula

FIGURE 8. Comparative photos of Pelodytes punctatus in life. The upper two photos show a male and a female from near Montpellier, France; the lower two pictures show a male and a female from near Vic, Girona, Spain. Specimens not collected. Images not to scale. Identity of P. punctatus individuals assessed by geographical location; specimens not DNA barcoded.

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FIGURE 2 in Integration of molecular, bioacoustical and morphological data reveals two new cryptic species of Pelodytes (Anura, Pelodytidae) from the Iberian Peninsula

FIGURE 2. Median-joining networks of Western Palearctic Pelodytes based on six nuclear markers and mtDNA (mt-ND4+mt- CYB). Samples in the nDNA networks are colored according to their assignment to the four major clusters in the mtDNA network, corresponding to the species as color coded in Fig. 1. Each circle represents a different haplotype and its size is proportional to its relative frequency. Black dots represent inferred unsampled or extinct haplotypes. Markers RPL3 and Cherpint7 are represented at the same scale.

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FIGURE 7 in Integration of molecular, bioacoustical and morphological data reveals two new cryptic species of Pelodytes (Anura, Pelodytidae) from the Iberian Peninsula

FIGURE 7. Bioacoustic differences between lineages of Pelodytes occurring in the Iberian Peninsula. For raw data, see Supplementary Table S3. The boxplot shows the average number of B notes per call series, i.e, every call consisting of one A note and one or more B notes. Average values were first computed per individual, and then summarized across species. Differences are significant between P. hespericus sp. nov. and all other species, and between P. atlanticus sp. nov. and P. ibericus (ANOVA with Tukey's post-hoc test; see Results).

opennotspecifiedDec 2017View details →

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