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4 results for “Phyloseq”
Phyloseq R object accompanying the paper Temporal Dynamics Cloacal Microbiota 16S metataxonomics
<p>This ready to load <strong>phyloseq</strong> R S4 object contains the ASV table, taxonomy table and sample metadata. This data was build using the DaDa2 (version 1.6.0) and phyloseq (version 1.223) R packages using our raw MiSeq PE300 sequencing data deposited at NCBI-SRA under BioProject: PRJNA673103.</p> <p>The accompanying (peer-reviewed) scientific article can be found here: </p> <ul> <li>https://www.frontiersin.org/articles/10.3389/fmicb.2020.626713/abstract </li> <li>DOI: 10.3389/fmicb.2020.626713</li> </ul> <p> </p> <p><strong>Study/paper</strong> </p> <p>J. Schreuder, F.C. Velkers, A. Bossers, R.J. Bouwstra, W.F. de Boer, P. van Hooft, J.A. Stegeman, S.D. Jurburg.</p> <p>Associations between animal health and performance, and the host’s microbiota have been recently established. In poultry, changes in the intestinal microbiota have been linked to housing conditions and host development, but how the intestinal microbiota respond to environmental changes under farm conditions is less well understood. To gain insight into the microbial responses following a change in the host’s immediate environment, we monitored four indoor flocks of adult laying chickens three times over 16 weeks, during which two flocks were given access to an outdoor range, and two were kept indoors. To assess changes in the chickens’ microbiota over time, we collected cloacal swabs of 10 hens per flock and performed 16S rRNA gene amplicon sequencing.<br> The poultry house (i.e., the stable in which flocks were housed) and sampling time explained 9.2 % and 4.4 % of the variation in the microbial community composition of the flocks, respectively. Remarkably, access to an outdoor range had no detectable effect on microbial community composition, the variability of microbiota among chickens of the same flock, or microbiota richness, but the microbiota of outdoor flocks became more even over time. Fluctuations in the composition of the microbiota over time within each poultry house were mainly driven by turnover in rare, rather than dominant, taxa and were unique for each flock. We identified 16 amplicon sequence variants that were differentially abundant over time between indoor and outdoor housed chickens, however none were consistently higher or lower across all chickens of one housing type over time. Our study shows that cloacal microbiota community composition in adult layers is stable following a sudden change in environment, and that temporal fluctuations are unique to each flock. By exploring microbiota of adult poultry flocks within commercial settings, our study sheds light on how the chickens’ immediate environment affects the microbiota composition.</p>
16S Phyloseq R object accompanying the paper: Effects of storage methods on total bacterial count and microbial composition of bovine colostrum
<p>This ready to load <strong>phyloseq</strong> R S4 object contains the ASV table, taxonomy table and sample metadata (16S V3-V4). This data was build using the DaDa2 (version 1.12.1) and phyloseq (version 1.32) R packages using our raw Illumina MiSeq PE300 sequencing data deposited at NCBI-SRA under BioProject: PRJNA872909.</p> <p>The accompanying (peer-reviewed) scientific article can be found here: </p> <ul> <li>https://www.todo</li> <li>DOI: todo</li> </ul> <p> </p> <p><strong>Study/draft abstract:</strong></p> <p>Lisa Robbers, Hannes Bijkerk, Lars Ravesloot, Alex Bossers, Mirjam Nielen, Ruurd Jorritsma, Ad Koets, Lindert Benedictus</p> <p>Neonatal calves need to acquire passive immunity through maternal colostrum, as they are immunologically naïve and the structure of the bovine placenta does not allow passage of maternal antibodies during pregnancy. Milked colostrum is not initially sterile and may even contain high bacterial counts. Minimizing total bacterial counts in colostrum is generally advised, however bacterial quality of colostrum comprises more than just bacterial quantities, but also depends on the specific bacteria present. While duration and temperature of colostrum storage are known to affect total plate counts (TPC), less is known about the effects of storage on the actual bacterial composition of the TPC. We speculated that, depending on the storage conditions, colostrum is a substrate in which certain bacterial species can thrive affecting the quality of colostrum.</p> <p>We therefore aimed to characterize the effects of different colostrum storage methods on the composition of the viable, aerobic, microbial community. Colostrum samples were stored at different temperatures and for different durations. Next, bacterial growth was assessed using the aerobe plate count culture method, followed by 16S rRNA gene amplicon sequencing. Differences in the TPC bacterial compositions of the stored colostrum samples, as determined by 16S rRNA gene amplicon sequencing, were mostly explained by the variation in bacterial composition of the colostrum sample directly after milking. In line with earlier studies, the results from our study show that the TPC increased when colostrum was stored for 24 hours at room temperature, but not when stored in a refrigerator for the same duration. Community structure of the TPC of colostrum stored at room temperature for 24 hours and stored in a refrigerator for a week was significantly different from the baseline samples. The 16S rRNA sequencing results indicate this is because of increased numbers of <em>Enterobacteriaceae.</em> <em>Enterobacteriaceae</em> abundance in refrigerated samples seemed to remain stable for the first 24 hours, but increased drastically after one week. The results indicate that microbial composition of stored colostrum is mostly influenced by the composition of the colostrum sample directly after milking, which is most probably the result of contamination or other environmental influences during the milking process. This study provides a deeper insight in the changes in the microbial composition of colostrum TPC during practical storage conditions and provides a primer for more detailed research into the determinants of bacterial composition of colostrum and the linked health effects.</p>
Phyloseq 16S v3v4 object accompanying paper: Host genotype affects endotoxin release in excreta of broilers at slaughter age
<p>This ready to load <strong>phyloseq</strong> R S4 object contains the ASV table, taxonomy table and sample metadata. This data was build using the DaDa2 (version 1.18.0) and phyloseq (version 1.34.0) R packages using the SILVA 138.1 nr99 database and raw MiSeq PE300 sequencing data deposited at NCBI-SRA under BioProject: PRJNA975731.</p> <p>F. Marcato, J.M.J. Rebel, S.K. Kar, I. Wouters, D. Schokker, A. Bossers, F. Harders, J.W. van Riel, M. Wolthuis-Fillerup and I.C. de Jong. </p> <p>The main goal of the current study was to investigate whether or not it was possible to modulate the fecal microbiome and thereby reducing endotoxin concentrations in the excreta of broiler chickens. An experiment was carried out with a 2 x 2 x 2 factorial arrangement including 3 factors, 1) genetic strain (fast-growing Ross 308 vs. slower-growing Hubbard JA757), 2) no vs. combined use of probiotics and prebiotics in the diet and drinking water, 3) early feeding at the hatchery vs. non early feeding. A total of 624 Ross 308 and 624 Hubbard JA757 day-old male broiler chickens were included until d 37 and d 51 of age, respectively. Broilers (26 chicks per pen) were housed in a total of 48 pens, and there were 6 replicate pens/treatment group. Pooled cloacal swabs (10 chickens per pen) for microbiome and endotoxin analyses were collected at a target body weight (BW) of 200 g, 1 kg and 2.5 kg.</p>
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