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13 results for “Pistacia vera”
Fig. 4 in Direct somatic embryogenesis of drought resistance pistachio (Pistacia vera L.) and expression analysis of somatic embryogenesis-related genes
Fig. 4. Somatic embryos induction of P. vera L. Sarakhs variety from immature zygotic embryos under dark condition after 60 days. Somatic embryos exhibited by arrows (a) Somatic embryos in globular stage, (b) Somatic embryos in heart stage, (c) Cotyledonary somatic embryos. Bar 2 mm.
Fig. 6 in Direct somatic embryogenesis of drought resistance pistachio (Pistacia vera L.) and expression analysis of somatic embryogenesis-related genes
Fig. 6. Secondary somatic embryos produced from primay somatic embryos of P. vera L. Sarakhs variety. Bar 2 mm.
Fig. 8 in Direct somatic embryogenesis of drought resistance pistachio (Pistacia vera L.) and expression analysis of somatic embryogenesis-related genes
Fig. 8. Relative expression of selected embryogenesis-related genes on embryonic and non-embryonic samples of two genotypes (a: Sarakhs variety and b: Ghazvini cultivar) of P.vera L in three technical replication.ACT was used as a reference gene for data normalization.Mean value and standard deviation (SD) were presented for three biological replicates.The significance differences (p <.01) and non-significant differences showed with two stars and "ns", respectively.
PISTACIA VERA L. NING ERONDAN KELTIRILGAN NAVI KO`CHATLARINI YETISHTIRISH VA PISTA PLANTATSIYALARINI BARPO ETISHNING SAMARALI USULLARI
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Table 3 in Direct somatic embryogenesis of drought resistance pistachio (Pistacia vera L.) and expression analysis of somatic embryogenesis-related genes
<p><b>Table 3</b> Effect of different plant growth regulators on induction of somatic embryos in three pistachio genotypes' explants (immature zygotic embryos) under light condition.</p><table><tbody><tr><th></th><th>Induction of somatic embryos %</th><th></th><th>Number of embryos/explant</th><th></th></tr></tbody><tbody><tr><th></th><td>Ghazvini</td><td>Badami Riz</td><td>Sarakhs</td><td>Ghazvini</td><td>Badami Riz</td><td>Sarakhs</td></tr><tr><th>E1</th><td>23.22 ± 3.39 c</td><td>22.74 ± 1.46d</td><td>24.67 ± 1.45 c</td><td>4.3 ± 1.2b</td><td>12.33 ± 1.45b</td><td>8 ± 0.58b</td></tr><tr><th>E2</th><td>34.25 ± 0.92b</td><td>34.66 ± 0.22 c</td><td>35.31 ± 0.85b</td><td>12.33 ± 1.45a</td><td>45 ± 2.88 a</td><td>11.33 ± 1.45a</td></tr><tr><th>E6</th><td>43.75 ± 3.6ab</td><td>43.58 ± 0.87b</td><td>42.83 ± 2.80a</td><td>5.33 ± 0.88b</td><td>10 ± 1.15b</td><td>7.67 ± 0.88b</td></tr><tr><th>E11</th><td>50.00 ± 4.12a</td><td>49.95 ± 1.19a</td><td>48.67 ± 2.03a</td><td>4.67 ± 0.88b</td><td>9.33 ± 1.20b</td><td>6 ± 0.58b</td></tr></tbody></table><p>Data within a column followed by different letters indicate significantly differences according to the LSD test at p <.05.</p>
Table 2 in Direct somatic embryogenesis of drought resistance pistachio (Pistacia vera L.) and expression analysis of somatic embryogenesis-related genes
<p><b>Table 2</b> PCR primer sequences of selected somatic embryogenesis-related genes for qRT in pistachio.</p><table><tbody><tr><th>Gene name</th><th>Forward primer sequence 5′-3′</th><th>Reverse primer sequence 5′-3′</th><th>Tm (̊C)</th><th>Amplicon length (bp)</th></tr></tbody><tbody><tr><th><i>ACT</i></th><td>GTATCCACGAGACCACCTACA</td><td>GGAGCAACGACCTTGATCTTC</td><td>60</td><td>157</td></tr><tr><th><i>AGL15</i></th><td>GGAGCAATCAAGAGTACAGGAACAG</td><td>CACTTCAGGACTTGCAGAACTATGG</td><td>60</td><td>187</td></tr><tr><th><i>LEC</i></th><td>GATGTGGGGTCTCTTGGAAGGA</td><td>AGCCTGTTTTGCTTTACGAAATCTC</td><td>60</td><td>209</td></tr><tr><th><i>BBM</i></th><td>TCCCAATTAGCAACTACGAGAAAGAG</td><td>CTGATGATGCCTTGTAACACCAC</td><td>60</td><td>140</td></tr><tr><th><i>PKL</i></th><td>GTAGACATCGCACCCTCTTAACTG</td><td>GAGCCAGCATTTTATGAAGTCTTGA</td><td>60</td><td>174</td></tr></tbody></table>
Table 1 in Direct somatic embryogenesis of drought resistance pistachio (Pistacia vera L.) and expression analysis of somatic embryogenesis-related genes
<p><b>Table 1</b> Summary of different responses of zygotic immature embryos to different basal medium (BM1 and BM2), combination and concentration of plant growth regulators (PGRs), and light and dark conditions.The response of explants was in the form of callus (C), somatic embryogenesis (SE), and adventitious shoot (AS).The Basal media were BM1: MS mineral salts,B5 vitamins, 30 g L <i>−</i> 1 sucrose, BM2: half strength MS macro salts, B5 vitamins, 100 mg L <i>−</i> 1 L-glutamine, 500 mg L <i>−</i> 1 casein hydrolysate, and 30 g L <i>−</i> 1 sucrose.</p><table><tbody><tr><th>Coding</th><th>PGRs combinations (mg L <i>−</i> 1)</th><th>Light</th><th>Dark</th></tr></tbody><tbody><tr><th></th><td></td><td>BM1</td><td>BM2</td><td>BM1</td><td>BM2</td><td>BM1</td><td>BM2</td><td>BM1</td><td>BM2</td><td>BM1</td><td>BM2</td><td>BM1</td><td>BM2</td></tr><tr><th></th><td></td><td>C</td><td></td><td>SE</td><td></td><td>AS</td><td></td><td>C</td><td></td><td>SE</td><td></td><td>AS</td><td></td></tr><tr><th>E1</th><td>0</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td></tr><tr><th>E2</th><td>0.01 IBA</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>–</td><td>–</td><td>–</td></tr><tr><th>E3</th><td>0.01 IBA + 1 BA</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>+</td><td>–</td><td>–</td><td>–</td><td>–</td></tr><tr><th>E4</th><td>0.01 IBA + 2 BA</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>+</td><td>–</td><td>+</td><td>–</td><td>–</td></tr><tr><th>E5</th><td>0.01 IBA + 3 BA</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>+</td><td>–</td><td>–</td><td>–</td><td>–</td></tr><tr><th>E6</th><td>0.01 2,4-D</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>–</td><td>–</td><td>–</td></tr><tr><th>E7</th><td>0.01 2,4-D + 0.1 TDZ</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>–</td><td>–</td><td>–</td></tr><tr><th>E8</th><td>0.01 2,4-D + 0.25 TDZ</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>–</td><td>–</td><td>–</td></tr><tr><th>E9</th><td>0.01 2,4-D + 0.5 TDZ</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>–</td><td>–</td><td>–</td></tr><tr><th>E10</th><td>0.01 2,4-D + 1 TDZ</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>–</td><td>–</td><td>–</td></tr><tr><th>E11</th><td>0.01 NAA</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>–</td><td>–</td><td>–</td></tr><tr><th>E12</th><td>0.01 NAA + 1 BA</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>+</td><td>–</td><td>–</td><td>–</td><td>+</td></tr><tr><th>E13</th><td>0.01 NAA + 2 BA</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>+</td><td>–</td><td>–</td><td>–</td><td>+</td></tr><tr><th>E14</th><td>0.01 IBA + 0.1 TDZ</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>+</td><td>–</td><td>–</td><td>–</td><td>–</td></tr><tr><th>E15</th><td>0.01 IBA + 0.25 TDZ</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>+</td><td>–</td><td>–</td><td>–</td><td>–</td></tr><tr><th>E16</th><td>0.01 IBA + 0.5 TDZ</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>+</td><td>–</td><td>–</td><td>–</td><td>–</td></tr><tr><th>E17</th><td>0.01 IBA + 1 TDZ</td><td>–</td><td>–</td><td>–</td><td>–</td><td>–</td><td>+</td><td>–</td><td>+</td><td>–</td><td>+</td><td>–</td><td>–</td></tr></tbody></table><p>«–» and « + » represent nonexistence and existence the response on explants that cultured in intended media, respectively.</p>
Cell layer-specific modification of cell wall is associated with exo-mesocarp split in pistachio (Pistacia vera L.)
GEO Series GSE300184. Pistacia vera. 20 samples. Type: Expression profiling by high throughput sequencing.
Fig. 5 in Direct somatic embryogenesis of drought resistance pistachio (Pistacia vera L.) and expression analysis of somatic embryogenesis-related genes
Fig. 5. Longitudinal sections different developmental stages of somatic embryos in P. vera L. Sarakhs variety obtained from immature zygotic embryos under light condition: (a) Heart-shaped somatic embryos arising from epidermal cells (arrow), (b) Somatic embryos in torpedo stage (c, d) Early cotyledonary stage somatic embryo with shoot apical meristem (SAM) and no connection to vascular system (VS).
Fig. 1 in Direct somatic embryogenesis of drought resistance pistachio (Pistacia vera L.) and expression analysis of somatic embryogenesis-related genes
Fig. 1. Somatic embryos induced from immature zygotic embryos of P. vera L. Sarakhs variety after 30 days incubation in initiatioan medium and light condition. (a) Somatic embryos in globular stage, (b) Somatic embryos in heart stage, (c) Asynchronous somatic embryos, (d) Somatic embryo in torpedo stage (e) Cotyledonary somatic embryos with distinct root and shoot poles, and (f) Germinated somatic embryos (g) Acclimatized plants after transplanting for 8 weeks. Bar 2 mm.
Fig. 2 in Direct somatic embryogenesis of drought resistance pistachio (Pistacia vera L.) and expression analysis of somatic embryogenesis-related genes
Fig. 2. The effect of type and concentrations of plant growth regulators on percentage (a) and number of (b) somatic embryos developed from immature zygotic embryos of three genotypes of P. vera after 30 days under light condition. Data represented mean ± SE of three repeated experiments.
Fig. 3 in Direct somatic embryogenesis of drought resistance pistachio (Pistacia vera L.) and expression analysis of somatic embryogenesis-related genes
Fig. 3. Interaction of plant growth regoulators treatments and genotypes on adventitious shoots' percentage obtained from immature zygotic embryos of three genotypes of P.vera under light condition. Data represented mean ± SE of three repeated experiments. Treatments are included: (E1): control, without hormone, (E2): 0.01 mg L −1 IBA + 1 mg L −1 BA, (E3): 0.01 mg L −1 IBA + 2 mg L − 1 BA, (E4): 0.01 mg L −1 IBA + 3 mg L −1 BA, (E5): 0.01 mg L −1 IBA + 3 mg L −1 BA, (E12): 0.1 mg L −1 NAA + 1 mg L −1 BA, (E13): 0.01 mg L −1 NAA + 1 mg L −1 BA, (E14): 0.02 mg L −1 IBA + 0.1 mg L −1 TDZ, (E15): 0.02 mg L −1 IBA+ 0.25 mg L −1 TDZ, (E16): 0.02 mg L −1 IBA + 0.5 mg L −1 TDZ,(E17): 0.02 mg L −1 IBA + 1 mg L −1 TDZ.
Fig. 7 in Direct somatic embryogenesis of drought resistance pistachio (Pistacia vera L.) and expression analysis of somatic embryogenesis-related genes
Fig. 7. Flow chart of somatic embryogenesis system for pistachio. Bar 2 mm.
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