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662 results for “RNA Polymerase II”
G-quadruplex in the gene of the large subunit of plant RNA polymerase II: billion years old story
<p><strong>Supplementary material to the journal article</strong></p> <p>Consist of:</p> <p>Supplementary material S1: Analyzed <em>RPB1 </em>sequences in 40 plant species together with detailed characteristics and G-quadruplex prediction using four different computational approaches.</p> <p>Supplementary material S2: G4 locus is the most conserved within the <em>RPB1</em> gene (40 bp long potential G4 locus is the most conserved site in the whole ~ 6000 bp long <em>RPB1</em> gene. See the histogram below the alignment: the position of the G4 locus is depicted, together with the horizontal red dashed line indicating relative nucleotide conservation among aligned sequences of<em> RPB1</em>)</p> <p>Supplementary material S3: Multiple alignment of G4 locus of <em>RPB1</em> paralogs in <em>Arabidopsis thaliana </em>centered to G4 locus of <em>RPB1 </em></p> <p>Supplementary material S4: Modelled 3D structure of G4 from <em>Bathycoccus prasinos</em> in PDB format</p> <p>Supplementary material S5: Gel electrophoresis and ThT staining of the selected G4-forming sequences</p> <p>Supplementary material S6: All analyzed <em>RPB1</em> sequences in FASTA format</p> <p>Supplementary material S7: Aligned <em>RPB1</em> sequences in FASTA format</p> <p>Supplementary material S8: <em>RPB1</em> paralogs in <em>Arabidopsis thaliana</em></p> <p>Supplementary material S9: Spectral composition of light used in the UV experiment. Analysis of emitted light was performed by Ocean Optics (HR4000CG-UV-NIR, USA) device.</p> <p>Supplementary material S10: Difference CD spectra - comparison without and with previous UV treatment</p>
Data from: Live-cell single particle imaging reveals the role of RNA polymerase II in histone H2A.Z eviction
<p>The H2A.Z histone variant, a genome-wide hallmark of permissive chromatin, is enriched near transcription start sites in all eukaryotes. H2A.Z is deposited by the SWR1 chromatin remodeler and evicted by unclear mechanisms. We tracked H2A.Z in living yeast at single-molecule resolution, and found that H2A.Z eviction is dependent on RNA Polymerase II (Pol II) and the Kin28/Cdk7 kinase, which phosphorylates Serine 5 of heptapeptide repeats on the carboxy-terminal domain of the largest Pol II subunit Rpb1. These findings link H2A.Z eviction to transcription initiation, promoter escape and early elongation activities of Pol II. Because passage of Pol II through +1 nucleosomes genome-wide would obligate H2A.Z turnover, we propose that global transcription at yeast promoters is responsible for eviction of H2A.Z. Such usage of yeast Pol II suggests a general mechanism coupling eukaryotic transcription to erasure of the H2A.Z epigenetic signal.</p>
Supporting data for "Dynamics of RNA polymerase II and elongation factor Spt4/5 recruitment during activator-dependent transcription"
<p>Supporting data for</p> <p><strong>Dynamics of RNA polymerase II and elongation factor Spt4/5 recruitment</strong></p> <p><strong>during activator-dependent transcription </strong></p> <p>Grace A. Rosen<sup>a,1</sup>, Inwha Baek<sup>b,1</sup>, Larry J. Friedman<sup>a</sup>, Yoo Jin Joo<sup>b</sup>, Stephen Buratowski<sup>b,2</sup>, Jeff Gelles<sup>a,2</sup></p> <p><sup>a</sup>Department of Biochemistry, Brandeis University, Waltham, Massachusetts 02454, USA.</p> <p><sup>b</sup>Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115, USA.</p> <p><sup>1</sup>Equal contributions</p> <p><sup>2</sup>Corresponding authors: <a href="mailto:steveb@hms.harvard.edu">steveb@hms.harvard.edu</a>; +1 (617) 432-0696 (S.B.) and <a href="mailto:gelles@brandeis.edu">gelles@brandeis.edu</a>; +1 (781) 736-2377 (J.G.)</p> <p>See <strong>Source data index.pdf</strong> for description of files.</p>
Data for: A novel and ubiquitous miRNA-involved regulatory module ensures precise phosphorylation of RNA polymerase II and proper transcription
<p>Proper transcription orchestrated by RNA polymerase II (RNPII) is crucial for cellular development, which relies on the phosphorylation state of RNPII's carboxyl-terminal domain (CTD). Sporangia, developed from mycelia, are essential for the destructive oomycetes<em> Phytophthora</em>, remarkable transcriptional changes are observed during the morphological transition. However, how these changes are rapidly triggered and their relationship with the versatile RNPII-CTD phosphorylation remain enigmatic. Herein, we found that <em>Phytophthora</em> <em>capsici</em> had undergone an elevation of Ser5-phosphorylation in its uncanonical heptapeptide repeats of RNPII-CTD during sporangia development, which subsequently changed the chromosomal occupation of RNPII and primarily activated transcription of certain genes. A cyclin-dependent kinase,<em> </em>PcCDK7, was highly induced and phosphorylated RNPII-CTD during this morphological transition. Mechanistically, a novel DCL1-dependent microRNA, pcamiR1, was found to be a feedback modulator for the precise phosphorylation of RNPII-CTD by complexing with PcAGO1 and regulating the accumulation of PcCDK7. Moreover, this study revealed that the pcamiR1-CDK7-RNPII regulatory module is evolutionarily conserved and the impairment of the balance between pcamiR1 and <em>PcCDK7 </em>could efficiently reduce the growth and virulence of<em> P. capsici</em>. Collectively, this study uncovers a novel and evolutionarily conserved mechanism of transcription regulation that could facilitate correct development and identifies pcamiR1 as a promising target for disease control.</p>
Dataset 1. Functional genomic profiling of O-GlcNAc reveals its context-specific interplay with RNA polymerase II.
<p>This dataset is used in the bioformatic methods of the article <strong>Functional genomic profiling of O-GlcNAc reveals its context-specific interplay with RNA polymerase II </strong>(doi: 10.1186/s13059-025-03537-2). The corresponding code can be found in the repository https://github.com/descostesn/chromglycomethods.git</p>
Lattice kinetic Monte Carlo model to simulate RNA polymerase II clusters
<p>This data set includes Python scripts (numerical simulation and analysis) and already generated simulation data for RNA polymerase II clusters. RNA polymerase II particles as single lattice sites and chromatin with regulatory region as connected polymer.</p>
Microscopy data: interaction of the gene ripply1 with RNA polymerase II clusters during early zebrafish embryo development
<p>Microscopy image data containing fluorescently labeled gene loci, recruited RNA polymerase II, and elongating RNA polymerase II.</p> <p>This data set is for the gene <em>ripply1</em> and is obtained from fixed zebrafish embryos, collected at the developmental stages oblong, sphere, dome, 30% epiboly, and 50% epiboly (indicated in the file names). Data were recorded using an instant-SIM microscope (iSIM, VisiTech UK) with a 100X TIRF oil immersion objective (Nikon, NA 1.49, CFI SR HP Apo TIRF 100XAC Oil). Two independent experiments were performed (IF1, IF2 in the file name), for each experiment, two samples were prepared per stage and experiment (001, 002 in the file name).</p> <p>The image data are in the ND2 format (Nikon proprietary) and can be imported using the BioFormats importer (Open Microscopy Environment).</p>
Microscopy data: interaction of the gene klf2b with RNA polymerase II clusters during early zebrafish embryo development
<p>Microscopy image data containing fluorescently labeled gene loci, recruited RNA polymerase II, and elongating RNA polymerase II.</p> <p>This data set is for the gene <em>klf2b</em> and is obtained from fixed zebrafish embryos, collected at the developmental stages oblong, sphere, dome, 30% epiboly, and 50% epiboly (indicated in the file names). Data were recorded using an instant-SIM microscope (iSIM, VisiTech UK) with a 100X TIRF oil immersion objective (Nikon, NA 1.49, CFI SR HP Apo TIRF 100XAC Oil). Two independent experiments were performed (IF1, IF2 in the file name), for each experiment, one or two samples were prepared per stage and experiment (001, 002 in the file name).</p> <p>The image data are in the ND2 format (Nikon proprietary) and can be imported using the BioFormats importer (Open Microscopy Environment).</p>
Microscopy data: interaction of the gene foxd5 with RNA polymerase II clusters during early zebrafish embryo development
<p>Microscopy image data containing fluorescently labeled gene loci, recruited RNA polymerase II, and elongating RNA polymerase II.</p> <p>This data set is for the gene <em>foxd5</em> and is obtained from fixed zebrafish embryos, collected at the developmental stages oblong, sphere, dome, 30% epiboly, and 50% epiboly (indicated in the file names). Data were recorded using an instant-SIM microscope (iSIM, VisiTech UK) with a 100X TIRF oil immersion objective (Nikon, NA 1.49, CFI SR HP Apo TIRF 100XAC Oil). Two independent experiments were performed (IF1, IF2 in the file name), for each experiment, two or three samples were prepared per stage and experiment (001, 002 in the file name).</p> <p>The image data are in the ND2 format (Nikon proprietary) and can be imported using the BioFormats importer (Open Microscopy Environment).</p>
Microscopy data: interaction of the gene gadd45ga with RNA polymerase II clusters during early zebrafish embryo development
<p>Microscopy image data containing fluorescently labeled gene loci, recruited RNA polymerase II, and elongating RNA polymerase II.</p> <p>This data set is for the gene <em>gadd45ga</em> and is obtained from fixed zebrafish embryos, collected at the developmental stages oblong, sphere, dome, 30% epiboly, and 50% epiboly (indicated in the file names). Data were recorded using an instant-SIM microscope (iSIM, VisiTech UK) with a 100X TIRF oil immersion objective (Nikon, NA 1.49, CFI SR HP Apo TIRF 100XAC Oil). Two independent experiments were performed (IF1, IF2 in the file name), for each experiment, two samples were prepared per stage and experiment (001, 002 in the file name).</p> <p>The image data are in the ND2 format (Nikon proprietary) and can be imported using the BioFormats importer (Open Microscopy Environment).</p>
Microscopy data: interaction of the gene drll2 with RNA polymerase II clusters during early zebrafish embryo development
<p>Microscopy image data containing fluorescently labeled gene loci, recruited RNA polymerase II, and elongating RNA polymerase II.</p> <p>This data set is for the gene <em>drll2</em> and is obtained from fixed zebrafish embryos, collected at the developmental stages oblong, sphere, dome, 30% epiboly, and 50% epiboly (indicated in the file names). Data were recorded using an instant-SIM microscope (iSIM, VisiTech UK) with a 100X TIRF oil immersion objective (Nikon, NA 1.49, CFI SR HP Apo TIRF 100XAC Oil). Two independent experiments were performed (IF1, IF2 in the file name), for each experiment, two samples were prepared per stage and experiment (001, 002 in the file name).</p> <p>The image data are in the ND2 format (Nikon proprietary) and can be imported using the BioFormats importer (Open Microscopy Environment).</p>
Microscopy data: interaction of the gene iscub with RNA polymerase II clusters during early zebrafish embryo development
<p>Microscopy image data containing fluorescently labeled gene loci, recruited RNA polymerase II, and elongating RNA polymerase II.</p> <p>This data set is for the gene <em>iscub</em> and is obtained from fixed zebrafish embryos, collected at the developmental stages oblong, sphere, dome, 30% epiboly, and 50% epiboly (indicated in the file names). Data were recorded using an instant-SIM microscope (iSIM, VisiTech UK) with a 100X TIRF oil immersion objective (Nikon, NA 1.49, CFI SR HP Apo TIRF 100XAC Oil). Two independent experiments were performed (IF1, IF2 in the file name), for each experiment, two samples were prepared per stage and experiment (001, 002 in the file name).</p> <p>The image data are in the ND2 format (Nikon proprietary) and can be imported using the BioFormats importer (Open Microscopy Environment).</p>
Lattice kinetic Monte Carlo model to simulate RNA polymerase II clusters during stem cell differentiation
<p>This data set includes Python scripts (numerical simulation and analysis) and already generated simulation data for RNA polymerase II clusters during stem cell differentiation. It includes the whole data to recreate panels.</p>
Microscopy-based assessment of RNA polymerase II clusters during differentiation of cultured mouse embryonic stem cells
<p>This repository contains the data and MatLab analysis scripts of the analysis of RNA polymerase II clusters over the course of differentiation of cultured mouse embryonic stem cells (mESCs). The cells were induced towards differentiation by either withdrawal of lineage-inhibiting factors (-LIF) or by RHB treatment. Both treatments were carried out in duplicate in independent experiments. Time course data were obtained by collection of cells at 3, 6, 12, 24, and 48 hours following the beginning of induction. Control data from unindexed cells were obtained at 24 and 48 hours. Cells were cultured, treated, collected, and fixed at University Medical Center Göttingen by Yomna Gohar, Priya Kumar, and Carmelo Ferrai. Fixed cells were fluorescently labeled and microscopy images were recorded and analyzed at Karlsruhe Institute of Technology by Lennart Hilbert. </p> <p>To analyse date data, the raw image data are first extracted into MatLab-native files using the <a href="https://zenodo.org/api/files/2f2192f4-a05c-4969-ba0a-4f235b837709/MultiPosition_extraction_nd2.m">MultiPosition_extraction_nd2.m</a> script. The actual analysis is then carried out using the <a href="https://zenodo.org/api/files/2f2192f4-a05c-4969-ba0a-4f235b837709/ClusterAnalysis.m">ClusterAnalysis.m</a> script. Example microscopy images were produced using the <a href="https://zenodo.org/api/files/2f2192f4-a05c-4969-ba0a-4f235b837709/ExampleImages.m">ExampleImages.m</a> script. The extracted data can be reviewed using the <a href="https://zenodo.org/api/files/2f2192f4-a05c-4969-ba0a-4f235b837709/ReviewExtractedStacks.m">ReviewExtractedStacks.m</a> script.</p> <p>This is the main repository, containing the image analysis scripts alongside the links to the raw image data used in the analysis. The raw data are too large to be stored within this single repository, and therefore can be found at the following additional addresses.</p> <p>Differentiation by withdrawal of lineage-inhibiting factors (-LIF): <a href="https://doi.org/10.5281/zenodo.8013281">10.5281/zenodo.8013281</a></p> <p>Differentiation with addition of RHB for neuronal fate induction (RHB): <a href="https://doi.org/10.5281/zenodo.8013307">10.5281/zenodo.8013307</a></p>
Data from: Live-cell single particle imaging reveals the role of RNA polymerase II in histone H2A.Z eviction
Open the record for dataset details and reuse information.
Data for: A novel and ubiquitous miRNA-involved regulatory module ensures precise phosphorylation of RNA polymerase II and proper transcription
Open the record for dataset details and reuse information.
Microscopy of RNA Polymerase II clusters in live zebrafish embryos
<p>This data set contains raw image data and derived images illustrating clusters of RNA polymerase II as observed in zebrafish embryos. The clusters are labelled by fluorescently marked antibody fragments (Fab) that detect phosphorylation of the C-terminal roman of RNA polymerase II. Antibody fragments were provided by the laboratory of Hiroshi Kimura.</p>
Effect of hexanediol treatment on clusters of RNA polymerase II in zebrafish embryo primary cell culture
<p>This data set assesses changes in RNA polymerase II clusters upon treatment with 3% 1,6-hexanediol in zebrafish embryos. RNA polymerase was labeled by immunofluorescence, microscopy images were acquired by instant-SIM microscopy and analyzed using MatLab scripts and the bioformats importer. This data set contains the raw image data as well as all further analysis scripts.</p>
Structural insights into distinct mechanisms of RNA polymerase II and III recruitment to snRNA promoters - segmented EM density used in integrative modeling
<p>segmented cryoEM map and derived gaussian mixture model used in the integrative modeling of the human SNAPc complex </p>
Translin facilitates RNA polymerase II dissociation and suppresses genome instability during RNase H2- and Dicer-deficiency (data files)
<p>The conserved nucleic acid binding protein Translin contributes to numerous facets of mammalian biology and genetic diseases. It was first identified as a binder of cancer-associated chromosomal translocation breakpoint junctions leading to the suggestion that it was involved in genetic recombination. With a paralogous partner protein, Trax, Translin has subsequently been found to form a hetero-octomeric RNase complex that drives some of its functions, including passenger strand removal in RNA interference (RNAi). The Translin-Trax complex also degrades the precursors to tumour suppressing microRNAs in cancers deficient for the RNase III Dicer. This oncogenic activity has resulted in the Translin-Trax complex being explored as a therapeutic target. Additionally, Translin and Trax have been implicated a wider range of biological function ranging from sleep regulation to telomere transcript control. Here we reveal a Trax- and RNAi-independent function for Translin in dissociating RNA polymerase II from its genomic template, with loss of Translin function resulting in increased transcription-associated recombination and elevated genome instability. This provides genetic insight into the longstanding question of how Translin might influence chromosomal rearrangements in human genetic diseases and provides important functional understanding of an oncological therapeutic target.</p>
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