Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
80
datasets available to search
ShareScore release 0.9.0
Dataset results
80 results for “RNA extraction”
Additional data for publication: Simple protocol for combined extraction of exocrine secretions and RNA in small arthropods.
<p>Additional data and results are given in this repository. It contains the trimmed reads (fastp; raw reads also on SRA accession numbers SRR29851544-SRR29851549, Bioproject PRJNA1136254), full busco reports for individual transcriptomes, assembly of all six RNAseqs together (transcriptome as base for differential expression analysis) and results of salmon.</p>
Methods for Extracting and Characterizing RNA from Urine: for downstream PCR and RNAseq Analysis
<p>Readily accessible samples such as urine or blood are seemingly ideal for differentiating and stratifying patients, however, it has proven a daunting task to identify reliable biomarkers in such samples. Noncoding RNA holds great promise as a source of biomarkers distinguishing physiologic wellbeing or illness.</p>
Data from: Decentralizing cell-free RNA sensing with the use of low-cost cell extracts
<p>Data and DNA sequences for the publication "Decentralizing cell-free RNA sensing with the use of low-cost cell extracts" (https://www.biorxiv.org/content/10.1101/2021.05.29.446205v1)</p>
RNA extraction alternative method for SARS-CoV-2 molecular diagnosis
<p>The devastating outbreak of COVID-19 has posed serious challenges for the diagnostics laboratories, often facing global shortage of reagents and equipment. This study aimed at evaluating an additional RNA extraction method respect to those already recommended by WHO and CDC. A new protocol for RNA extraction from nasopharyngeal swab was set up, adapting the Qiagen RNeasy 96 plate for cell lines, and validated on a set of 96 clinical samples analyzed in parallel by a recommended method. The internal control and target genes analysis showed a good agreement between the two extraction methods, indicating that the two approaches can be considered equivalent for the SARS-CoV-2 diagnostics. The addition of this extraction method can help in increasing the throughput for SARS-CoV-2 molecular test, even in a low automation setting.</p> <p>The data set published in Zenodo is the full data analysed in the paper.</p>
A novel methodological approach to simultaneously extract high quality total RNA and proteins from cortical and trabecular bone
<p>Molecular differences between cortical and trabecular bone, of relevance to understand the pathophysiological bases of bone diseases, can be determined only throughout effective isolation methods for RNA and proteins. Here we present a TRIzol-based method, combining bone pulverization and homogenization, to extract simultaneously total RNA and proteins from human cortical and trabecular bone from the same carrot. RNA integrity and purity were determined as the 260-to-280 nm and 260-to-230 nm absorbance- ratios, and 28S-to-18S rRNA ratio. Protein integrity and quality were evaluated by Comassie Blue staining. RT-qPCR and immunoblotting for bone-specific genes and proteins were performed to verify the suitability of the isolated material in downstream applications. 260-to-280 nm and 260-to-230 nm absorbance ratios were, on average, ≥1.8. Bands on agarose gel were consistent to an intact RNA, with mean 28S-to-18S ratios of1.68±0.35 and 1.88±0.10 for cortical and trabecular bone, respectively. Band patterns after Comassie Blue staining confirmed protein integrity. Successful gene and protein expression analysis, with relevant differences between the two compartments, highlighted the suitability of the material in downstream applications. The method here presented is appropriate and effective for the study of human bone.</p>
Small RNA-seq from FPLC-fractionated leaf and flower crude extracts
GEO Series GSE130431. Arabidopsis thaliana. 16 samples. Type: Non-coding RNA profiling by high throughput sequencing.
RNA-seq analysis of cymopol and algal extracts in the intestine
GEO Series GSE107623. Mus musculus. 15 samples. Type: Expression profiling by high throughput sequencing.
Examination and comparison of the RNA extraction methods using mouse serum
GEO Series GSE246437. Mus musculus. 3 samples. Type: Non-coding RNA profiling by array.
End Sequence Analysis ToolKit (ESAT) expands the extractable information from single cell RNA-Seq data
GEO Series GSE79651. Mus musculus; Rattus norvegicus. 14 samples. Type: Expression profiling by high throughput sequencing.
Affymetrix GeneChip Mouse Gene 1.0 arrays of RNA extracted from gastrocnemius muscle of 4 global Ppp3cb KO mice and 4 corresponding WT littermates, and 4 skeletal muscle-specific Ppp3r1Mlc1fCre KO an
GEO Series GSE71174. Mus musculus. 16 samples. Type: Expression profiling by array.
RNA-biotin based pull down assay with Hela Nuclear Extract followed by RNA-seq
GEO Series GSE98218. Homo sapiens. 2 samples. Type: Other; Expression profiling by high throughput sequencing.
RNA immunoprecipitation (RIP) of IGF2BP3 from pancreatic cancer cell extracts cultured on fibronectin
GEO Series GSE47597. Homo sapiens. 2 samples. Type: Other.
RNA-SEQ analysis of single cells extracted from the renal vesicles of P4 Crym-EGFP transgenic mice
GEO Series GSE59130. Mus musculus. 57 samples. Type: Expression profiling by high throughput sequencing.
Multiple approaches converge on three biological subtypes of meningioma and extract new insights from published studies [RNA-Seq]
GEO Series GSE189672. Homo sapiens. 110 samples. Type: Expression profiling by high throughput sequencing.
RNA-sequencing of milk cells extracted from pre-partum secretions and longitudinally from mature human milk across the first year of lactation
GEO Series GSE85494. Homo sapiens. 21 samples. Type: Expression profiling by high throughput sequencing.
RNA extracted from the left ventricle of 3 different mouse heart disease models, wild type, mdx, and mdx sln+/- hearts
GEO Series GSE144596. Mus musculus. 8 samples. Type: Expression profiling by high throughput sequencing.
RNA-SEQ analysis of single cells extracted from E12.5 embryonic kidneys.
GEO Series GSE59127. Mus musculus. 86 samples. Type: Expression profiling by high throughput sequencing.
SnapShot-Seq: a method for extracting genome-wide, in vivo mRNA dynamics from a single total RNA sample
GEO Series GSE48889. Homo sapiens. 28 samples. Type: Expression profiling by high throughput sequencing.
PTex - Purification of Cross-linked RNA-Protein Complexes by Phenol-Toluol Extraction
GEO Series GSE113655. Homo sapiens. 23 samples. Type: Expression profiling by high throughput sequencing.
Straightforward extraction of high-quality RNA from glyoxal-fixed cells facilitates transcriptome analysis after cell staining and sorting by flow cytometry
GEO Series GSE158177. Homo sapiens. 12 samples. Type: Expression profiling by high throughput sequencing.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.