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90 results for “RT-PCR”
Diagnostic accuracy of a set of clinical and radiological criteria for screening of COVID-19 using RT-PCR as the reference standard - Dataset
<p>Dataset of a cohort whose summary is described below.</p> <p>Abstract</p> <p><strong>Objective:</strong> To evaluate the accuracy, sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of a set of clinical-radiological criteria for COVID-19 screening in patients with severe acute respiratory failure (SARF) admitted to intensive care units (ICUs), using reverse-transcriptase polymerase chain reaction (RT-PCR) as the reference standard. <strong>Method: </strong>Diagnostic accuracy study including a historical cohort of 1009 patients consecutively admitted to ICUs across six hospitals in Curitiba (Brazil) from March to September, 2020. The sample was stratified into groups by the strength of suspicion for COVID-19 (strong <em>versus</em> weak) using parameters based on three clinical and radiological (chest computed tomography) criteria. The diagnosis of COVID-19 was confirmed by RT-PCR (referent). <strong>Results:</strong> With respect to RT-PCR, the proposed criteria had 98.5% (95% confidence interval [95% CI] 97.5–99.5%) sensitivity, 70% (95% CI 65.8–74.2%) specificity, 85.5% (95% CI 83.4–87.7%) accuracy, PPV of 79.7% (95% CI 76.6–82.7%) and NPV of 97.6% (95% CI 95.9–99.2%). <strong>Conclusion: </strong>The proposed set of clinical-radiological criteria were accurate in identifying patients with strong <em>versus</em> weak suspicion for COVID-19 and had high sensitivity and considerable specificity with respect to RT-PCR. These criteria may be useful for screening COVID-19 in patients presenting with SARF.</p>
Research Data for Comparative Evaluation of RT-PCR and Antigen-based Rapid Diagnostic Tests (Ag-RDTs) for SARS-CoV-2 Detection: Performance, Variant Specificity, and Clinical Implications
<p>This dataset represents laboratory findings for the comparative evaluation of the diagnostic performance of Ag-RDTs (Flourescence Immunoassay and Lateral Flow Immunoassay) with RT-PCR</p>
Data and scripts from Epidemiological and clinical insights from SARS-CoV-2 RT-PCR crossing threshold values, France, January to November 2020
<p>Raw data and scripts used in the publication "<em>Epidemiological and clinical insights from SARS-CoV-2 RT-PCR crossing threshold values, France, January to November 2020 separator commenting unavailable</em>" in Eurosurveillance in 2022.</p> <p> </p> <p>https://www.eurosurveillance.org/content/10.2807/1560-7917.ES.2022.27.6.2100406</p>
Splicing patterns of the Arabidopsis organellar rhomboid At1g74140 - Table S1 for primers used in the RT-PCR assays
<p>Extended Data for the publication: "Analysis of the <em>Arabidopsis</em> organellar rhomboid At1g74140 transcript population uncovered splicing patterns different from its close relative At1g74130"</p> <p>Supplementary Table S1 - List of At1g74140 primers used in the different RT-PCR assays. </p>
Real-time RT-PCR datasets for the CEO study
<p>Real-time RT-PCR results of testing citrus essential oils for viroid transmission. </p> <p>Ct values of each run are listed in Excel sheet according to the respective experiment. </p>
Investigation of the diagnostic importance and accuracy of CT in the chest compared to the RT-PCR test for suspected COVID-19 patients in Jordan
<p>As the world continues to battle the COVID-19 pandemic, Jordan is no exception. In order to improve the accuracy of diagnosis, researchers in Jordan have conducted an investigation into the diagnostic importance and accuracy of CT in the chest compared to the RT-PCR test for suspected COVID-19 patients.</p>
Efficacy of a Multiplex BangasureTM RT-PCR Kit for the Detection of COVID-19
ClinicalTrials.gov study NCT05190016. IPD Sharing: NO. Countries: 1. Publications: 14.
A Study of an Ad26.RSV.preF-based Regimen in the Prevention of Reverse Transcriptase Polymerase Chain Reaction (RT-PCR)-Confirmed Respiratory Syncytial Virus (RSV)-Mediated Lower Respiratory Tract Dis
ClinicalTrials.gov study NCT03982199. IPD Sharing: YES. Countries: 1. Publications: 2.
MicroRNA quantitative RT-PCR analysis of CMT1A Plasma
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SARS-CoV-2 nucleocapsid and RT-PCR results
<p><span>We collected blood samples from patients admitted to the hospital during a period with RT-PCR based-screening of patients for Severe Acute Respiratory Syndrome Cornavirus-2 (SARS-CoV-2). Retrospectively the SARS-CoV-2 nucleocapsid protein (NP) plasma concentrations were measured with an ELISA method and used for an initial time course study to find the optimal time-point for sampling blood. Next, we estimated the diagnostic accuracy i.e. the clinical sensitivity and specificity at different plasma NP cut-off concentrations.</span></p> <p><span>The time course study revealed profiles with rapid or more slow declines in NP titers after the RT-PCR result. Nevertheless, in the time interval 0 – 7 days after the RT-PCR result, the NP concentration was always above the level of detection at 1.66 pg/ml suggesting that the diagnosis could be established in the time interval of 0 - 7 days.</span></p> <p><span>The median time gap between the plasma NP and RT-PCR results was 0.0 days (n = 1957, interval: -26 to + 21 days). Reducing the time gap to seven days, the clinical sensitivity was 90.0% (n= 60, 95% CI, 82.4% to 97.6%) at a specificity of 95.9% (n=1876, 95% CI, 95.0% to 96.8%). Curve analysis by receiver operation characteristics identified a cut-off concentration of 1.87 pg/mL NP as optimal resulting in a positive predictive value of 41.2%, a negative predictive value of 99.7% and a prevalence of 3.1%.</span></p> <p><span>In conclusion, the NP method is acceptable for making the laboratory diagnosis of SARS-CoV-2, and an intended use of plasma NP as a prospective nosocomial screening method is considered feasible.</span></p>
Contamination of Testicle Tissue by RT-PCR in Participants With Solid Tumors
ClinicalTrials.gov study NCT02400970. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Comparison of Strategies Based on RT-PCR or Antigenic Test for the Screening of SARS-CoV-2 Infection (COVID-19).
ClinicalTrials.gov study NCT04859023. IPD Sharing: NO. Countries: 1. Publications: 1.
Prognostic Value of a Positive RT-PCR Test in Patients With Ewing Sarcoma
ClinicalTrials.gov study NCT00339898. IPD Sharing: Not stated. Countries: 1. Publications: 3.
Performance Evaluation of RealDetect™ COVID-19 RT-PCR Kit for the Detection of SARS-CoV-2 Virus
ClinicalTrials.gov study NCT04403672. IPD Sharing: NO. Countries: 1. Publications: 2.
Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Buccal Screening Evaluation Using an RT-PCR Assay and a Rapid ELISA Test Among Symptomatic and Asymptomatic Patients
ClinicalTrials.gov study NCT05074745. IPD Sharing: NO. Countries: 1. Publications: 1.
Self-Collected Saliva Samples Without Viral Transport Media for COVID-19 Testing Via RT-PCR
ClinicalTrials.gov study NCT04604145. IPD Sharing: Not stated. Countries: 1. Publications: 4.
Implementation of an RT-PCR Assay for the Diagnosis of Rickettsia Spp. Infection
ClinicalTrials.gov study NCT07307651. IPD Sharing: Not stated. Countries: 1. Publications: 2.
RT-PCR on Urine Samples: Added Value for the Diagnosis of Chikungunya Virus Infection
ClinicalTrials.gov study NCT02714985. IPD Sharing: NO. Countries: 2. Publications: 10.
SARS-CoV-2 nucleocapsid and RT-PCR results
Open the record for dataset details and reuse information.
Oligonucleotide primers used for RT-PCR
<p>Oligonucleotide primers used for RT-PCR</p>
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Allen Brain Atlas
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.