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34 results for “Ralstonia solanacearum”
Phage selection drives resistance-virulence trade-offs in Ralstonia solanacearum plant pathogenic bacterium irrespective of the growth temperature
<p><span>While temperature has been shown to affect the survival and growth of bacteria and their phage parasites, it is unclear if trade-offs between phage resistance and other bacterial traits depend on the temperature. Here, we experimentally compared the evolution of phage resistance-virulence trade-offs and underlying molecular mechanisms in phytopathogenic <em>Ralstonia</em> <em>solanacearum</em> bacterium at 25 °C and 35 °C temperature environments. We found that experimental growth conditions selected for small colony variants (SCVs) with increased growth rate and mutations in the quorum-sensing (QS) signalling receptor gene, <em>phcS</em>. Interestingly, SCVs were also phage-resistant and reached higher frequencies in the presence of phages in both temperature environments. Evolving phage resistance was costly in terms of reduced carrying capacity, biofilm formation and reduced virulence i<em>n planta</em> possibly due to loss of QS-mediated expression of key virulence genes. We also observed mucoid phage-resistant colonies that showed loss of virulence and reduced twitching motility likely due to parallel mutations in prepilin peptidase gene pilD. Moreover, phage-resistant SCVs from 35 °C-phage treatment had parallel mutations in genes encoding type II secretion system (T2SS) genes (<em>gspE</em> and <em>gspF</em>), indicating that defects in pseudopilus made bacterium resistant to the phage. Additional transcriptomic analysis revealed upregulation of CBASS and type Ⅰ restriction-modification phage defence systems in response to phage exposure, which coincided with reduced expression of motility and virulence-associated genes, including <em>pilD</em> and type II and III secretion systems. Together, these results suggest that phage resistance-virulence trade-offs are not affected by the growth temperature but can be mediated through both pre- and post-infection phage resistance mechanisms.</span></p>
Phage selection drives resistance-virulence trade-offs in Ralstonia solanacearum plant pathogenic bacterium irrespective of the growth temperature
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Data from: Plant pathogenic bacterium Ralstonia solanacearum can rapidly evolve tolerance to antimicrobials produced by Pseudomonas biocontrol bacteria
<p>Soil-borne plant pathogens significantly threaten crop production due to lack of effective control methods. One alternative to traditional agrochemicals is microbial biocontrol, where pathogen growth is suppressed by naturally occurring bacteria that produce antimicrobial chemicals. However, it is still unclear if pathogenic bacteria can evolve tolerance to biocontrol antimicrobials and if this could constrain the long-term efficacy of biocontrol strategies. Here we used an <em>in vitro</em> experimental evolution approach to investigate if the phytopathogenic <em>Ralstonia solanacearum </em>bacterium, which causes bacterial wilt disease, can evolve tolerance to antimicrobials produced by <em>Pseudomonas</em> bacteria. We further asked if tolerance was specific to pairs of <em>R. solanacearum</em> and <em>Pseudomonas</em> strain and certain antimicrobial compounds produced by <em>Pseudomonas</em>. We found that while all <em>R. solanacearum</em> strains could initially be inhibited by <em>Pseudomonas</em> strains, this inhibition decreased following successive subculturing with or without <em>Pseudomonas</em> supernatants. Using separate tolerance assays, we show that the majority of <em>R. solanacearum </em>strains evolved increased tolerance to multiple <em>Pseudomonas</em> strains. Mechanistically, evolved tolerance was most likely linked to reduced susceptibility to orfamide lipopeptide antimicrobials secreted by <em>Pseudomonas</em> strains in our experimental conditions. Some levels of tolerance also evolved in the control treatments, which was likely correlated response due to adaptations to the culture media. Together, these results suggest that plant-pathogenic bacteria can rapidly evolve increased tolerance to bacterial antimicrobial compounds, which could reduce the long-term efficacy of microbial biocontrol.</p>
Ralstonia solanacearum ̶ Pest Report and Datasheet to support ranking of EU candidate priority pests
<p>These two files are part of the outputs produced under the mandate <a href="http://registerofquestions.efsa.europa.eu/roqFrontend/wicket/page?1-1.ILinkListener-contentPane-listContainer-pageable-21-mandateNumberLnk">M-2017-0056</a> of the European Commission requesting EFSA for technical assistance in the field of quarantine pests qualifying as priority pests as by Article 6(2) of the Regulation (EU) 2016/2031 <em>on protective measures against pests of plants</em>.</p> <p>Under the mandate EFSA produced: i) 1 methodology report (DOI available at the field "Related/alternate identifiers"), ii) 28 datasheets, one for each of the 28 candidate pests, and iii) 28 pest reports supporting the information provided in the datasheets.</p> <p> EFSA wishes to acknowledge the contribution of Marie-Agnes Jacques, Trond Rafoss, Gregor Urek, Dirkjan van der Gaag, Jonathan Yuen to the EKE and the review conducted by Christian Vernière.</p>
Data from: Plant pathogenic bacterium Ralstonia solanacearum can rapidly evolve tolerance to antimicrobials produced by Pseudomonas biocontrol bacteria
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Dataset - A complex network of additive and epistatic quantitative trait loci underlies natural variation of Arabidopsis thaliana quantitative disease resistance to Ralstonia solanacearum under heat stress
<p>Plant immunity is often negatively impacted by heat stress. However, the underlying molecular mechanisms remain poorly characterized. Based on a genome-wide association mapping approach, this study aims to identify in <em>Arabidopsis thaliana</em> the genetic bases of robust resistance mechanisms to the devastating pathogen<em> Ralstonia solanacearum</em> under heat stress. A local mapping population was phenotyped against the <em>R. solanacearum</em> GMI1000 strain at 27 and 30 °C. To obtain a precise description of the genetic architecture underlying natural variation of quantitative disease resistance (QDR), we applied a genome-wide local score analysis. Alongside an extensive genetic variation found in this local population at both temperatures, we observed a playful dynamics of quantitative trait loci along the infection stages. In addition, a complex genetic network of interacting loci could be detected at 30 °C. As a first step to investigate the underlying molecular mechanisms, the atypical meiotic cyclin <em>SOLO DANCERS</em> gene was validated by a reverse genetic approach as involved in QDR to <em>R. solanacearum </em>at 30 °C. In the context of climate change, the complex genetic architecture underlying QDR under heat stress in a local mapping population revealed candidate genes with diverse molecular functions.</p>
Dataset - A complex network of additive and epistatic quantitative trait loci underlies natural variation of Arabidopsis thaliana quantitative disease resistance to Ralstonia solanacearum under heat stress
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Assembly and annotation of Solanum dulcamara and Solanum nigrum plant genomes, two nightshades with contrasting susceptibilities to Ralstonia solanacearum [RNAseq_expression_tissues]
GEO Series GSE283679. Solanum dulcamara. 5 samples. Type: Expression profiling by high throughput sequencing.
Expression analysis of Ralstonia solanacearum strain GMI1000 at 20°C and 28°C in rich medium (CPG) and in planta
GEO Series GSE33657. Ralstonia pseudosolanacearum GMI1000. 16 samples. Type: Expression profiling by array; Expression profiling by genome tiling array.
Master transcription factors of plant immunity are targeted by a Ralstonia solanacearum effector
GEO Series GSE179700. Arabidopsis thaliana. 12 samples. Type: Expression profiling by high throughput sequencing.
Expression analysis of Ralstonia solanacearum strain UW551 at 20°C and 28°C in rich medium (CPG) and in planta
GEO Series GSE33661. Ralstonia solanacearum UW551. 16 samples. Type: Expression profiling by array; Expression profiling by genome tiling array.
Assembly and annotation of Solanum dulcamara and Solanum nigrum plant genomes, two nightshades with contrasting susceptibilities to Ralstonia solanacearum
GEO Series GSE262401. Solanum dulcamara. 1 samples. Type: Methylation profiling by high throughput sequencing.
RNA-seq analysis of Ralstonia solanacearum nitrosative stress response mutants
GEO Series GSE194210. Ralstonia solanacearum. 9 samples. Type: Expression profiling by high throughput sequencing.
Shared and unique transcriptomic changes in chickpea in response to concurrent drought stress and Ralstonia solanacearum pathogen
GEO Series GSE89228. Cicer arietinum. 16 samples. Type: Expression profiling by array.
Assembly and annotation of Solanum dulcamara and Solanum nigrum plant genomes, two nightshades with contrasting susceptibilities to Ralstonia solanacearum
GEO Series GSE255584. Solanum nigrum. 1 samples. Type: Methylation profiling by high throughput sequencing; Other.
A Ralstonia solanacearum effector targets splicing factor SR34a to reprograms alternative splicing and regulates host immunity
GEO Series GSE276633. Solanum lycopersicum. 6 samples. Type: Expression profiling by high throughput sequencing.
Impact of pehR transcriptional regulator on Ralstonia solanacearum F1C1 gene expression patterns
GEO Series GSE269108. Ralstonia solanacearum. 2 samples. Type: Expression profiling by high throughput sequencing.
Ralstonia solanacearum_GMI1000 and UW551_20 C and 28 C.
GEO Series GSE33662. Ralstonia solanacearum UW551; Ralstonia pseudosolanacearum GMI1000. 32 samples. Type: Expression profiling by array; Expression profiling by genome tiling array.
RNA-seq analysis of Ralstonia solanacearum treated with nitrosative and oxidative stress
GEO Series GSE160024. Ralstonia solanacearum. 24 samples. Type: Expression profiling by high throughput sequencing.
Global transcriptome and targeted metabolite analyses of roots reveal different defense mechanisms against Ralstonia solanacearum infection in two resistant potato cultivars
GEO Series GSE211973. Solanum tuberosum. 16 samples. Type: Expression profiling by high throughput sequencing.
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