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14 results for “Respirometry”
Energetic costs increase with faster heating in an aquatic ectotherm: Respirometry data
<p>Using closed chamber respirometry we estimated the aerobic metabolic rate of an aquatic ectotherm, the Atlantic ditch shrimp <em>Palaemonetes varians,</em> under varying thermal conditions. We continuously measured oxygen consumption of shrimp during heating, cooling, and constant temperatures, starting trials at a range of acclimation temperatures and exposing shrimp to a variety of rates of temperature change.</p> <p>Data corrections and associated methodologies are detailed fully in the accompanying manuscript. </p> <p>## Description of the data and file structure</p> <p>Stable temperature trial data found in stable_trials_raw.csv</p> <p>Ramping temperature trial data found in ramping_trials_raw.csv</p> <p>Both datasets include the respirometry data for all temperature trials, with dissolved oxygen concentration corrected for background respiration ("doconc_corr") following the methodology outlined in the associated manuscript. Identification number of the individual is stored under "ID", time from start of trial to end of trial (in minutes) is stored under "time_min" and temperature of the chamber (in degrees Celcius) is stored under "temp_degC".</p>
Respirometry data for cactus mice (Peromyscus eremicus) corresponding to Colella et al. 2021
<p>Metabolism is a complex phenotype shaped by natural environmental rhythms, as well as behavioral, morphological, and physiological adaptations. Metabolism has been historically studied under constant environmental conditions, but new methods of continuous metabolic phenotyping now offer a window into organismal responses to dynamic environments, and enable identification of abiotic controls and the timing of physiological responses relative to environmental change. We use indirect calorimetry to characterize metabolic phenotypes of the desert-adapted cactus mouse (<i>Peromyscus eremicus</i>) in response variable environmental conditions that mimic their native environment versus those recorded under constant warm and constant cool conditions, while using a constant photoperiod and full access to resources. We found significant sexual dimorphism, with males being more prone to dehydration than females. Under circadian environmental variation, most metabolic shifts occurred prior to physical environmental change and the timing was disrupted under both constant temperature-humidity treatments. The ratio of CO<sub>2</sub> produced to O<sub>2</sub> consumed (the respiratory quotient) reached greater than 1.0, only during the light phase under diurnally variable conditions, a pattern that strongly suggests that<i> </i>lipogenesis is contributing to the production of energy and endogenous water. Our results are consistent with historical descriptions of circadian torpor in this species (torpid by day, active by night), but reject the hypothesis that torpor is initiated by food restriction or negative water balance.</p>
Respirometry data for cactus mice (Peromyscus eremicus) corresponding to Colella et al. 2021
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Respirometry protocols for avian thermoregulation at high air temperatures: stepped and steady-state profiles yield similar results
<p>Relationships between air temperature (Tair) and avian body temperature (Tb), resting metabolic rate (RMR) and evaporative water loss (EWL) during acute heat exposure can be quantified through respirometry using several approaches. One involves birds exposed to a stepped series of progressively increasing Tair setpoints for short periods (< 20-30 min), whereas a second seeks to achieve steady-state conditions by exposing birds to a single Tair for longer periods (> 1-2 h). To compare these two approaches, we measured Tb, RMR and EWL over Tair = 28 C to 44 C in the dark-capped bulbul (Pycnonotus tricolor). The two protocols yielded indistinguishable values of Tb, RMR and EWL and related variables at most Tair values, revealing that both are appropriate for quantifying avian thermal physiology during heat exposure over the range of Tair in the present study. The stepped protocol, however, has several ethical and practical advantages. </p>
Respirometry protocols for avian thermoregulation at high air temperatures: stepped and steady-state profiles yield similar results
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The ankle–brachial index, gastrocnemius mitochondrial respirometry, and walking performance in people with and without peripheral artery disease
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High-resolution respirometry in a small-volume chamber
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Stability of mitochondrial respiration medium used in high-resolution respirometry with living and permeabilized cells
<p>Excel and DatLab files</p>
Respirometry data
<p>Respirometry data for article: Do air-breathing fish suffer branchial oxygen loss in hypoxic water?.</p> <p>The excel file has the data used and raw data is also available in the Acqknowledge files.</p> <p>3 files per replicate (normoxia, hypoxia and kali = background measurements and CO2 calibrations).</p> <p>For control data also 3 files ( Norm 1, Norm 2 and kali.</p>
Snow bunting respirometry data
<p>1. Arctic animals inhabit some of the coldest environments on the planet and have evolved physiological mechanisms for minimizing heat loss under extreme cold. However, the Arctic is warming faster than the global average and how well Arctic animals tolerate even moderately high air temperatures (T<sub>a</sub>) is unknown.</p> <p>2. Using flow-through respirometry we investigated the heat tolerance and evaporative cooling capacity of snow buntings (<i>Plectrophenax nivalis</i>; ≈ 31g, N = 42), a cold specialist, Arctic songbird. We exposed buntings to increasing T<sub>a</sub> and measured body temperature (T<sub>b</sub>), resting metabolic rate (RMR), rates of evaporative water loss (EWL) and evaporative cooling efficiency (the ratio of evaporative heat loss to metabolic heat production).</p> <p>3. Buntings had an average (±SD) T<sub>b</sub> of 41.3 ± 0.2 °C at thermoneutral T<sub>a</sub>, and increased T<sub>b</sub> to a maximum of 43.5 ± 0.3 °C. Buntings started panting at T<sub>a</sub> of 33.2 ± 1.7 °C, with rapid increases in EWL starting at T<sub>a</sub> = 34.6 °C, meaning they experienced heat stress when air temperatures were well below their body temperature. Maximum rates of EWL were only 2.9x baseline rates at thermoneutral T<sub>a</sub>, a markedly lower increase than seen in more heat tolerant arid-zone species (e.g., ≥ 4.7x baseline rates). Heat stressed buntings also had low evaporative cooling efficiencies, with 95% of individuals unable to evaporatively dissipate an amount of heat equivalent to their own metabolic heat production.</p> <p>4. Our results suggest that buntings' well-developed cold tolerance may come at the cost of reduced heat tolerance. As the Arctic warms, and this and other species experience increased periods of heat stress, a limited capacity for evaporative cooling may force birds to increasingly rely on behavioural thermoregulation, such as minimizing activity, at the expense of diminished performance or reproductive investment.</p>
Oxygen measurements in closed system respirometry using blubber explants from suckling and fasting grey seal pups
<p>Understanding physiological responses of wildlife and domesticated animals to environmental challenges can be difficult or impossible to investigate at the whole animal level. Tissue culture approaches open up experimental possibilities, but are difficult to undertake in remote environments where facilities to assess tissue viability and minimise or monitor microbial contamination may not be available. Here we used planar optodes in closed system respirometry to measure oxygen use by blubber explants from grey seal (Halichoerus grypus) pups, obtained in a remote field environment in 2017 and processed in a field laboratory with minimal equipment. These data show oxygen measurements at timed intervals and demonstrate that the tissues were respiring aerobically for the 24 h measurement period, compared to empty control vials. We also measured glucose, lactate and glycerol levels in culture media after 24 hours to allow correlation of glucose uptake, lactate accumulation and lipolytic rate, which are also useful markers of metabolic activity, with oxygen use data. Nutritional state of the pups (either suckling or fasting) and tissue depth (closer to muscle (inner) or closer to skin (outer)) were recorded to allow effects of tissue characteristics on metabolic parameters to be examined. We were also able to use the oxygen use profiles in explant-containing and control vials to identify those contaminated by microbes well ahead of any visible colour change in the media stocks. The use of planar optodes for oxygen measurement thus allows simultaneous physiological measurements and contamination monitoring to be undertaken in a minimally equipped field laboratory. Robinson et al. (2021) published in Methods in Ecology and Evolution provides the details of tissue collection and tissue culture. These data will facilitate comparison of metabolic activity of adipose tissue within and between species and assessment of efficacy of monitoring microbial contamination in tissue culture experiments, particularly in field laboratories where other methods may not be logistically possible.</p>
Evaluation of Mitochondrial Function in Myofascial Trigger Points Cohort Pilot Study Using High-resolution Respirometry
ClinicalTrials.gov study NCT03704311. IPD Sharing: NO. Countries: 0. Publications: 1.
Snow bunting respirometry data
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Oxygen measurements in closed system respirometry using blubber explants from suckling and fasting grey seal pups
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Allen Brain Atlas
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OpenNeuro
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