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162 results for “Rotavirus”
Rotavirus viroplasms
<p>Rotavirus cell infection and immunofluorescence.</p> <p>MA-104 Clone 1 cells (American Type Culture Collection; ATCC:CRL-2378.1; RRID:CVCL_3846) were cultured in DMEM-RS media supplemented with 5% fetal bovine serum at 37°C and 5% CO<sup>2</sup>. Prior to infection, Rhesus rotavirus (RRV) was activated with trypsin (10 μg/ml) for 30 min at 37°C. MA104 cells grown on glass coverslips were infected with RRV at a multiplicity of infection (MOI) of 1. Cells were fixed and prepared for immunofluorescence after six hours post-infection. Using a STORM imaging buffer mounting medium (1.5% glucose oxidase + 100 mM β-mercaptoethanol), the coverslips were mounted onto the center of glass slides.</p> <p>Rotavirus replication machinery imaging.</p> <p>Cell infection micrographs were provided kindly by Garcés and collaborators [1]. Imaging was carried out with an Olympus IX-81 inverted microscope in TIRF mode (Olympus, cellTIRFM illuminator), with an evanescence field depth of 200 nm. The objective lens used was an Olympus UApo N 100x 1.4 NA oil-immersion, with an additional 1.6x magnification lens. Excitation of Alexa-488 and Alexa-568 was provided by 488 nm and 568 nm lasers, respectively. Using a laser-modulation protocol, previously described in [1], an EMCCD camera (iXon 897, Model No: DU-897E-CS0-#BV; Andor) was used to acquire the images. Acquisition rate was set to 20 fps with a pixel size of 100 nm. For processing with MSSR, the algorithm parameters were set as follows: AMP = 5, PSF = 3, Order = 1, GPU parallel computing = enabled, temporal analysis = Mean (100 frames).</p> <p>1. Garcés S, Y. et al. Nanoscale organization of rotavirus replication machineries. elife 8, e42906, (2019).</p>
A Phase II Dose-ranging Study of Oral RV3-BB Rotavirus Vaccine
ClinicalTrials.gov study NCT03483116. IPD Sharing: YES. Countries: 1. Publications: 2.
Data and code from: Cost-effectiveness Analysis of Alternative Infant and Neonatal Rotavirus Vaccination Schedules in Malawi
Open the record for dataset details and reuse information.
Rotavirus infection and health related quality of life of hospitalized children in Latvia
<p>Data on Rotavirus infection and health related quality of life of hospitalized children in Latvia, 527 respondents, anonymous data, data collected: April 2013 to December 2015.</p>
Data from: Rotavirus NSP1 subverts the antiviral oligoadenylate synthetase-RNase L pathway by inducing RNase L degradation
<div>The interferon (IFN)-inducible 2′,5′-oligoadenylate synthetase (OAS)-RNase L pathway plays a critical role in antiviral immunity. Group A rotaviruses, including the simian SA11 strain, inhibit this pathway through two activities: an E3-ligase related activity of NSP1 that degrades proteins necessary for IFN signaling, and a phosphodiesterase (PDE) activity of VP3 that hydrolyzes the RNase L-activator 2′,5′-oligoadenylate. Unexpectedly, we found that a recombinant (r) SA11 double mutant virus deficient in both activities (rSA11-VP3H797R-NSP1ΔC17) retained the ability to prevent RNase L activation. Mass spectrometry led to the discovery that NSP1 interacts with RNase L in rSA11-infected HT29 cells. This interaction was confirmed through copulldown assay of cells transiently expressing NSP1 and RNase L. Immunoblot analysis showed that infection with wild-type rSA11 virus, rSA11-VP3H797R-NSP1ΔC17 double mutant virus, or single mutant forms of the latter virus all resulted in the depletion of endogenous RNase L. The loss of RNase L was reversed by addition of the neddylation inhibitor MLN4924, but not the proteasome inhibitor MG132. Analysis of additional mutant forms of rSA11 showed that RNase L degradation no longer occurred when either the N-terminal RING domain of NSP1 was mutated or the C-terminal 98 amino acids of NSP1 were deleted. The C-terminal RNase L degradation domain is positioned upstream and is functionally independent of the NSP1 domain necessary for inhibiting IFN expression. Our studies reveal a new role for NSP1 and its E3-ligase related activity as an antagonist of RNase L and uncover a novel virus-mediated strategy of inhibiting the OAS-RNase L pathway.</div>
Coadministration of Measles-rubella and Rotavirus Vaccines
ClinicalTrials.gov study NCT01700621. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Reactogenicity and Safety of GlaxoSmithKline (GSK) Biologicals' Rotarix™ (Human Rotavirus Vaccine) in Indian Infants
ClinicalTrials.gov study NCT00938327. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Exploration of the Biologic Basis for Underperformance of Oral Polio and Rotavirus Vaccines in Bangladesh
ClinicalTrials.gov study NCT01375647. IPD Sharing: Not stated. Countries: 1. Publications: 14.
Evaluation of Immunogenicity and Safety of Two Formulations of GSK Biologicals' Human Rotavirus (HRV) Vaccine (444563), in Healthy Infants Starting at Age 6-12 Weeks
ClinicalTrials.gov study NCT02914184. IPD Sharing: YES. Countries: 8. Publications: 1.
To Assess Safety, Reactogenicity & Immunogenicity of 2 Doses of GSK's Oral Human Rotavirus Vaccine in Pre-Term Infants
ClinicalTrials.gov study NCT00420745. IPD Sharing: YES. Countries: 4. Publications: 1.
Immune Response to Rotavirus Vaccine After a Supplemental Dose Given at 9 Months of Age With Local EPI Vaccines in Mali
ClinicalTrials.gov study NCT02286895. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Evaluation of the Human Rotavirus Vaccine at Varying Schedules and Doses in Rural Ghana
ClinicalTrials.gov study NCT01575197. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Study to Assess the Efficacy, Immunogenicity and Safety of Liquid Human Rotavirus Vaccine, in Healthy Chinese Infants
ClinicalTrials.gov study NCT01171963. IPD Sharing: YES. Countries: 1. Publications: 2.
Safety and Immunogenicity Study of Trivalent P2-VP8 Subunit Rotavirus Vaccine in Adults, Toddlers and Infants
ClinicalTrials.gov study NCT02646891. IPD Sharing: NO. Countries: 1. Publications: 1.
A Phase 1 Dose Escalation Study to Examine the Safety of the P2-VP8 Rotavirus Vaccine
ClinicalTrials.gov study NCT01764256. IPD Sharing: Not stated. Countries: 1. Publications: 1.
The Effects of Increased Inoculum on Oral Rotavirus Vaccine Take and Immunogenicity
ClinicalTrials.gov study NCT02992197. IPD Sharing: NO. Countries: 1. Publications: 1.
To Evaluate Safety & Reactogenicity of GSK Bio's Human Rotavirus (HRV) Vaccine in Filipino Infants at Least 6 Weeks of Age at First Vaccination
ClinicalTrials.gov study NCT00353366. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Safety and Immunogenicity of Sequential Rotavirus Vaccine Schedules
ClinicalTrials.gov study NCT01266850. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Rotavirus Vaccine Produced by Butantan Institute
ClinicalTrials.gov study NCT00981669. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Evaluation of Immunogenicity and Safety of the Diphtheria, Tetanus, Pertussis and Inactivated Poliovirus (DPT-IPV) Vaccine Squarekids Co-administered With GSK Biologicals' Human Rotavirus (HRV) Vaccin
ClinicalTrials.gov study NCT02907216. IPD Sharing: YES. Countries: 1. Publications: 1.
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Allen Brain Atlas
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.