Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
29
datasets available to search
ShareScore release 0.9.0
Dataset results
29 results for “Spo11”
Mass spectrometry data Spo11 and Msh4-1
<p>The procedure for Spo11 data is based on the peptide intensities reported in the evidence file provided by MaxQuant:</p> <p>1) peptide intensities are normalized using the variance stabilization transformation (bioconductor package vsn)</p> <p>2) Doing Top3, I chose not to impute any further the missing peptide intensities</p> <p>3) Taking the protein groups identified by MaxQuant and reported in the proteinGroup file, the protein intensities are calculated as the mean of the three most intense peptide of the leading razor protein.</p> <p>4) If no protein is found in a sample, the reported intensity is zero</p> <p>5) Differential analysis using the empirical Bayes statistics from the bioconductor limma packed is performed with the false discovery rate set at 0.01. Most proteins showing significant difference are only in SPO11.</p> <p>MaxQuant proteinGroups and evidence file are joined as sheets in the Mass Spec Spo11_raw.xlsx. The first sheet, "Differential Analysis", marks the proteins significantly different in SPO versus CTR (first column), under the test condition described above. The next 6 columns are the Top3 intensities in each sample. You may also want to ignore proteins with Q>0 (column BL in the file).</p> <p> </p> <p> </p>
Data from: High throughput sequencing reveals alterations in the recombination signatures with diminishing spo11 activity
Spo11 is the topoisomerase-like enzyme responsible for the induction of the meiosis-specific double strand breaks (DSBs), which initiate the recombination events responsible for proper chromosome segregation. Nineteen PCR-induced alleles of SPO11 were identified and characterized genetically and cytologically. Recombination, spore viability and synaptonemal complex (SC) formation were decreased to varying extents in these mutants. Arrest by ndt80 restored these events in two severe hypomorphic mutants, suggesting that ndt80-arrested nuclei are capable of extended DSB activity. While crossing-over, spore viability and synaptonemal complex (SC) formation defects correlated, the extent of such defects was not predictive of the level of heteroallelic gene conversions (prototrophs) exhibited by each mutant. High throughput sequencing of tetrads from spo11 hypomorphs revealed that gene conversion tracts associated with COs are significantly longer and gene conversion tracts unassociated with COs are significantly shorter than in wild type. By modeling the extent of these tract changes, we could account for the discrepancy in genetic measurements of prototrophy and crossover association. These findings provide an explanation for the unexpectedly low prototroph levels exhibited by spo11 hypomorphs and have important implications for genetic studies that assume an unbiased recovery of prototrophs, such as measurements of CO homeostasis. Our genetic and physical data support previous observations of DSB-limited meioses, in which COs are disproportionally maintained over NCOs (CO homeostasis).
Data from: High throughput sequencing reveals alterations in the recombination signatures with diminishing spo11 activity
Open the record for dataset details and reuse information.
Spo11 generates gaps through concerted cuts at sites of topological stress
GEO Series GSE171046. Saccharomyces cerevisiae; Saccharomyces kudriavzevii; Nakaseomyces glabratus. 71 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Spo11 generates gaps through concerted cuts at sites of topological stress [Top2]
GEO Series GSE169760. Saccharomyces cerevisiae; Nakaseomyces glabratus. 8 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Genome-wide mapping of meiotic double-strand breaks by sequencing Spo11 oligos.
GEO Series GSE26449. Saccharomyces cerevisiae. 4 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Nucleotide resolution mapping of Spo11-linked DNA breaks in the yeast genome
GEO Series GSE137685. Saccharomyces cerevisiae. 7 samples. Type: Other.
Spo11-accessory proteins link DNA double-strand break sites to the chromosome axis in early meiotic recombination
GEO Series GSE29860. Saccharomyces cerevisiae. 34 samples. Type: Genome binding/occupancy profiling by genome tiling array.
Supression of genetic recombination in the pseudoautosomal region and at subtelomeres in mice with a hypomorphic spo11 allele
GEO Series GSE48493. Mus musculus. 9 samples. Type: Genome variation profiling by high throughput sequencing; Other.
Physical interaction with Spo11 mediates the localisation of Mre11 to chromatin in meiosis and promotes its nuclease activity
GEO Series GSE253302. Saccharomyces cerevisiae; Nakaseomyces glabratus. 12 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Reconstitution of SPO11-dependent double-strand break formation
GEO Series GSE275291. Mus musculus; Cloning vector pUC19; Saccharomyces cerevisiae; Escherichia coli. 14 samples. Type: Other.
Spo11-oligo mapping in wild type S. cerevisiae strain
GEO Series GSE119689. Saccharomyces cerevisiae. 4 samples. Type: Other.
Spo11 generates gaps through concerted cuts at sites of topological stress [dDSB]
GEO Series GSE171042. Saccharomyces cerevisiae; Saccharomyces kudriavzevii. 63 samples. Type: Other.
Fun30-Myc ChIP-seq in Spo11 wild type and spo11-yf mutants
GEO Series GSE221033. Saccharomyces cerevisiae. 16 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Genome-wide mapping of meiotic DSB induced by Gal4BD-Spo11
GEO Series GSE5884. Saccharomyces cerevisiae. 14 samples. Type: Genome binding/occupancy profiling by genome tiling array.
Meiotic double-strand break mapping in spo11 mutants by S1Seq
GEO Series GSE150313. Saccharomyces cerevisiae. 4 samples. Type: Other.
Spo11-oligo mapping in S. cerevisiae Ctf19/CCAN kinetochore sub-complex mutant mcm21
GEO Series GSE72683. Saccharomyces cerevisiae. 2 samples. Type: Other.
Spo11-oligo mapping in Saccharomyces species (S. paradoxus, S. mikatae, S. kudriavzevii) and wild-derived S. cerevisiae strains (YPS128, UWOPS03-461.4)
GEO Series GSE71887. Saccharomyces cerevisiae; Saccharomyces kudriavzevii; Saccharomyces mikatae; Saccharomyces paradoxus. 10 samples. Type: Other.
ATM and PRDM9 Regulate SPO11-bound Recombination Intermediates During Meiosis
GEO Series GSE138915. Mus musculus. 32 samples. Type: Genome binding/occupancy profiling by high throughput sequencing; Other.
Spo11-oligo mapping in S. cerevisiae red1, hop1, mek1 mutants
GEO Series GSE84859. Saccharomyces cerevisiae. 6 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.