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697 results for “Staphylococcus aureus”
Dataset: Label-free detection of methicillin resistance in Staphylococcus aureus using different Raman-spectroscopy approaches
<p>This is the dataset accompanying the submission of the manuscript: Label-free detection of methicillin resistance in Staphylococcus aureus using different Raman-spectroscopy approaches in the journal Microbiology Spectrum.</p> <p>The data description is the following:</p> <p>Strains<br> 16859MRSA= Strain AUSTR-07-16859 MRSA<br> 16859MSSA= Strain AUSTR-07-16859 MSSA<br> CC8MRSA= Strain 08V15773<br> CC8MSSA= Strain MRSA2010-174<br> AUSTR05MRSA= Strain AUSTR-05-15441 MRSA<br> AUSTR05MSSA= Strain AUSTR-05-15441 MSSA<br> CC361MRSA= Strain UAE-Abu Dhabi-020<br> CC361MSSA= Strain UAE-Dubai-80-MS 1368.9/09</p> <p>Datasets<br> UVRR: UV-Resonance Raman with 244 nm excitation on bulk samples, calibration standard Polystyrene, measurements were time series of 10 consecutive spectra, for each strain and batch 25 time series were collected from 3 different slides<br> 532nm: Single cell analysis with 532nm excitation, calibration standard 4AAP, one spectrum per bacterial cell was collected<br> 785nm: Bulk analysis of bacterial colonies using 785 nm excitation and a Raman fibre probe, calibration standard 4AAP, bulk analysis, individual spectra of colonies were collected</p> <p>Data structure is in the metadata files.<br> Individual spectra are in the folders sorted by the date they were measured.</p>
A Niclosamide-releasing hot-melt extruded catheter prevents Staphylococcus aureus experimental biomaterial-associated infection
<p>Biomaterial-associated infections are a major healthcare challenge as they are responsible for high disease burden in critically ill patients. In this study, we have developed drug-eluting antibacterial catheters to prevent catheter-related infections. Niclosamide (NIC), originally an antiparasitic drug, was incorporated into the polymeric matrix of thermoplastic polyurethane (TPU) via solvent casting, and catheters were fabricated using hot-melt extrusion technology. The mechanical and physicochemical properties of TPU polymers loaded with NIC were studied. NIC was released in a sustained manner from the catheters and exhibited <em>in vitro</em> antibacterial activity against <em>Staphylococcus aureus</em> and <em>Staphylococcus epidermidis</em>. Moreover, the antibacterial efficacy of NIC-loaded catheters was validated in an <em>in vivo</em> biomaterial-associated infection mouse model using a methicillin-susceptible and methicillin-resistant strain of <em>S. aureus. </em>The released NIC from the produced catheters reduced bacterial colonization of the catheter as well as of the surrounding tissue. In summary, the NIC-releasing hot-melt extruded catheters prevented implant colonization and reduced the bacterial colonization of peri-catheter tissue by methicillin sensitive as well as resistant <em>S. aureus</em> in a biomaterial-associated infection mouse model and has good prospects for preclinical development.</p>
Dataset - Nasal microbiome disruption and recovery after mupirocin treatment in Staphylococcus aureus carriers and noncarriers
<p><strong>Background. </strong>Nasal decolonization procedures against the opportunistic pathogen <em>Staphylococcus aureus</em> rely on topical antimicrobial drug usage, whose impact on the nasal microbiota is poorly understood. We examined this impact in healthy <em>S. aureus </em>carriers and noncarriers.</p> <p> </p> <p><strong>Methods. </strong>This is a prospective interventional cohort study of 8 <em>S. aureus </em>carriers and 8 noncarriers treated with nasal mupirocin and chlorhexidine bath. Sequential nasal swabs were taken over 6 months. <em>S. aureus</em> was detected by quantitative culture and genotyped using <em>spa</em> typing. RNA-based 16S species-level metabarcoding was used to assess the living microbial diversity.</p> <p> </p> <p><strong>Results. </strong>The species <em>Dolosigranulum pigrum, Moraxella nonliquefaciens </em>and<em> Corynebacterium propinquum </em>correlated negatively with <em>S. aureus</em> carriage. Mupirocin treatment effectively eliminated <em>S. aureus, D. pigrum </em>and<em> M. nonliquefaciens, </em>but not corynebacteria. <em>S. aureus</em> recolonization in carriers occurred more rapidly than recolonization by the dominant species in noncarriers (median 3 vs. 6 months, respectively). Recolonizing <em>S. aureus</em> isolates had the same <em>spa</em> type as the initial isolate.</p> <p> </p> <p><strong>Conclusions. </strong>Mupirocin-chlorhexidine treatment had a long-lasting impact on the nasal microbiota. <em>S. aureus</em> recolonization predated microbiota recovery, emphasizing the strong adaptation of this pathogen to the nasal niche and the transient efficacy of the decolonization procedure.</p>
METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS AND ITS DETERMINANTS OF RAW COW MILK CONTAMINATION IN SELECTED GAMO ZONE DISTRICT OF SOUTHERN ETHIOPIA
<p>We conducted this study, titled "Methicillin-Resistant Staphylococcus aurous and its Determinants of Raw Cow Milk Contamination in a Selected Gamo Zone District of Southern Ethiopia," in order to disseminate our findings to the scientific community. The research has its own strengths, such as the work we did on milk quality and potential milk borne pathogens, as well as the determinants of contamination at the selling point, which is only on fresh milk after it has been checked for freshness. As a result, the authors initiated this research based on practical challenges encountered while providing professional support to various producers in the milk selling point. Because of the irrational use of antibiotics among human and animal health in the study area, this research aimed to estimate the microbial load, prevalence of methicillin resistant <em>S. aurous</em> (MRSA), and determinants of raw cow milk contamination at selling points. This could be a source of drug resistance development in Ethiopia and in the study area in particular. Furthermore, due to resource constraints, the study focuses on bacteriological analysis, risk factor association, and drug sensitivity testing, which can serve as baseline data for future characterization and intervention. Hoping that we authors are interested to publish our research output in your journal with your eminent support for the article gets published.</p>
Staphylococcus aureus Centrifuge DB
<p>Plascope DB for Staphylococcus aureus. It can be used to perform binary classification of plasmid contigs.</p>
Staphylococcus aureus and non-aureus staphylococci and mammaliicocci strains (NASM) used in our study.
<p><strong>Supplementary Table 1.</strong> <em>Staphylococcus aureus </em>and non-<em>aureus</em> staphylococci and mammaliicocci strains (NASM) used in our study.</p>
Figure 6 in Blue and red light photoemitters as approach to inhibit Staphylococcus aureus and Pseudomonas aeruginosa growth
Figure 6. Percentage of growth inhibition induced by blue light on bacteria inoculated on saline solution or nutrient rich BHI broth. *Statistically significant difference using Mann-Whitney U test (p <0.05) between blue light exposed S. aureus in saline solution and BHI broth.
Figure 5 in Blue and red light photoemitters as approach to inhibit Staphylococcus aureus and Pseudomonas aeruginosa growth
Figure 5. Effect of blue and red light on S. aureus e P. aeruginosa diluted in BHI nutrient rich medium applied for a period of 3 hours. *Statistically significant difference using Mann-Whitney U test (p <0.05) between blue light exposed and control groups.
Figure 3 in Blue and red light photoemitters as approach to inhibit Staphylococcus aureus and Pseudomonas aeruginosa growth
Figure 3. Determination of the influence of glass or polystyrene plate on the antimicrobial effect of red or blue light in S. aureus and P. aeruginosa cultures. *Statistically significant difference using Mann-Whitney U test (p <0.05) between blue light exposed group and control groups.
Figure 4 in Blue and red light photoemitters as approach to inhibit Staphylococcus aureus and Pseudomonas aeruginosa growth
Figure 4. Effect of blue and red light on S. aureus e P. aeruginosa diluted in saline solution (0.9% NaCl) applied for a period of 3 hours. *Statistically significant difference using Mann-Whitney U test (p <0.05) between blue light exposed group and control groups.
Figure 2 in Blue and red light photoemitters as approach to inhibit Staphylococcus aureus and Pseudomonas aeruginosa growth
Figure 2. Diameter of S.aureus and P. aeruginosa surviving colonies after exposure to blue and red light for 6 hours and incubated for 24 hours (A) and 48 hours (B). *Statistically significant difference using Mann-Whitney U test (p <0.05) for independent samples.
Figure 1 in Effect of Mauritia flexuosa L. leaf extract on Staphylococcus aureus and Staphylococcus haemolyticus biofilms adhered to stainless steel surface
Figure 1. Gas chromatographic profile of extracts from Mauritia flexuosa L. leaves. (A) Ethanolic extract and (B) Aqueous extract. The peaks numbers correspond to the phenolic compounds and carbohydrates listed in Table 1.
HOLO Dataset - Energy Landscape of the Domain Movement in Staphylococcus aureus UDP-N-acetylglucosamine 2-epimerase
<p>Results of the Molecular Dynamic simulations performed for the paper entitled "Energy Landscape of the Domain Movement in Staphylococcus aureus UDP-N-acetylglucosamine 2-epimerase". The trajectories and the prmtop file here are without the solvent and ions.</p>
APO Dataset - Energy Landscape of the Domain Movement in Staphylococcus aureus UDP-N-acetylglucosamine 2-epimerase
<p>Results of the Molecular Dynamic simulations performed for the paper entitled "Energy Landscape of the Domain Movement in Staphylococcus aureus UDP-N-acetylglucosamine 2-epimerase". The trajectories and the prmtop file here are without the solvent and ions.</p>
Kinetic in support of "Pre-Steady-State Kinetic Characterization of an Antibiotic-Resistant Mutant of Staphylococcus aureus DNA Polymerase PolC"
<p>These are KinTek Explorer mechanism files containing the data and analysis described in the manuscript "Pre-Steady-State Kinetic Characterization of an Antibiotic-Resistant Mutant of Staphylococcus aureus DNA Polymerase PolC" (bioRxiv 2022.10.04.510889; doi: https://doi.org/10.1101/2022.10.04.510889). </p>
A simplified method for comprehensive capture of the Staphylococcus aureus proteome: S. aureus proteome data table
Open the record for dataset details and reuse information.
Staphylococcus aureus VCF files: Risk Factors for Recurrent Staphylococcus aureus Bacteremia
<p>Background: To understand the clinical, bacterial, and host characteristics associated with recurrent Staphylococcus aureus bacteremia (R-SAB), patients with R-SAB were compared to contemporaneous patients with a single episode of SAB (S-SAB).<br> Methods: All SAB isolates underwent spa genotyping. All isolates from R-SAB patients underwent Pulsed-Field Gel Electrophoresis (PFGE). PFGE-indistinguishable pairs from 40 patients underwent whole genome sequencing (WGS). Acute phase plasma from R-SAB and S-SAB patients was matched 1:1 for age, race, gender, and bacterial genotype and underwent cytokine quantification using 25-analyte multiplex bead array. Results: R-SAB occurred in 69 (9.1%) of the 756 study patients. Of the 69 patients, 30 experienced relapse (43.5%) and 39 reinfection (56.5%). Age, race, hemodialysis dependence, presence of foreign body, MRSA, and persistent bacteremia were individually associated with likelihood of recurrence. Multivariate risk modeling revealed that African American (AA) hemodialysis patients were nearly two times more likely (OR = 9.652 [5.402 – 17.418]) than White hemodialysis patients (OR = 4.53 [1.696 – 10.879]) to experience R-SAB. WGS confirmed PFGE interpretations in all cases. Median RANTES levels in acute phase plasma from the initial episode of SAB were higher in R-SAB than in matched S-SAB controls ( P = 0.0053, False Discovery Rate < 0.10). <br> Conclusion: This study identified several risk factors for R-SAB. The largest risk for R-SAB is among AA hemodialysis patients. Higher RANTES levels in R-SAB compared to<br> matched controls warrants further study.</p>
Staphylococcus aureus phenol soluble modulin aggregation kinetics
<p><span>The infective ability of the </span>opportunistic pathogen <i><span>S</span>taphylococcus<span> aureus</span></i><span>, recognized as the most frequent cause of biofilm-associated infections, is associated with biofilm mediated resistance to host immune response. Phenol-soluble modulins (PSM) comprise the structural scaffold of <i>S. aureus</i> biofilms through self-assembly into functional amyloids, but the role of individual PSMs during biofilm formation remains poorly understood and the </span>molecular pathways of PSM self-assembly have yet to be identified<span>. Here, we demonstrate high degree of cooperation between individual PSMs during functional amyloid formation. PSMα3 initiates the aggregation, forming unstable aggregates capable of seeding other PSMs resulting in stable amyloid structures. Using chemical kinetics we dissect the molecular mechanism of aggregation of individual PSMs showing that PSMα1, PSMα3 and PSM</span><span>β</span><span>1 display secondary nucleation whereas PSM</span><span>β</span><span>2 aggregates through primary nucleation and elongation. Our findings suggest that the various PSMs have evolved to ensure fast and efficient biofilm formation through cooperation between individual peptides.</span></p>
Staphylococcus aureus isolated from ruminants with mastitis in northern Greece dairy herds: genetic relatedness and phenotypic and genotypic characterization
<p>Figure S1: Dendrogram of SmaI PFGE pulsotypes (P) and characteristics of the 162 S. aureus isolates.</p>
Genomics polymorphisms of Staphylococcus aureus strain NCTC 8325 in the lab stock maintained at TUM (WT), after 30 passes in BHI media (D) and after 30 passes detecting 4 -fold MIC increase to isocyanide -code I16- 3 biological replicates (A,B,C), and 3 independent colonies sequenced per replicate at the end of the experiment.
<p>Genomics polymorphisms of Staphylococcus aureus strain NCTC 8325 in the lab stock maintained at TUM (WT), after 30 passes in BHI media (D) and after 30 passes detecting 4 -fold MIC increase to isocyanide -code I16- 3 biological replicates (A,B,C), and 3 independent colonies sequenced per replicate at the end of the experiment. Determined from Illumina shotgun genomic sequencing datasets, mapping and analyses vs the reference genome of the strain https://www.ncbi.nlm.nih.gov/nuccore/NC_007795.1/</p>
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.