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21 results for “TRPA1”
Modulation of glia activation by TRPA1 antagonism in a pre-clinical model of migraine
<p>This dataset comprises the findings obtained in the study aimed at investigating the relationship between TRPA1 channels and glia activation in the modulation of trigeminal hyperalgesia in preclinical models of migraine.</p> <p><strong><em>In vivo</em></strong> assessments performed in male Sprague-Dawley rats four hours after treatment with nitroglycerin (10mg/kg, i.p.) or its vehicle in the acute model, and 24h after the last treatment with nitroglycerin (5mg/kg, i.p.) or its vehicle in the chronic model (injections every other day over a 9-day period for a total of five injections):</p> <p>- evaluation of trigeminal hyperalgesia in the orofacial formalin test: the face rubbing was measured counting the seconds the animal spent grooming the injected area (upper lip, lateral to the nose) with the ipsilateral forepaw or hindpaw 0–3 min (Phase I) or 12–45 min (Phase II) after formalin injection (50 µl, s.c.). The observation time was divided into 15 blocks of 3 min each.</p> <p><strong><em>Ex vivo</em></strong> assessments performed on samples collected at the end of the behavioral test:</p> <p>- Microglia and astroglia activation was evaluated the trigeminal nucleus caudalis by means of immunofluorescence staining. Specifically, the medullary segment containing the trigeminal nucleus caudalis (between +1 and -5 mm from the obex) was removed and cut transversely at 30 µm thickness on a freezing sliding microtome. The anti-cluster of differentiation molecule 11b (anti-CD11b, 1:300) primary antibody (to detect microglia) or rabbit anti-glial fibrillary acidic protein (anti-GFAP, 1:500) primary antibody (to label astrocytes) were used. Cell count for microglia and astroglia was assessed by counting CD11b- or GFAP-positive cells, respectively, from a stack of 16 pictures (1μm-thick, 20x magnification) taken from four representative sections alongside the trigeminal nucleus caudalis. The total number of CD11b- and GFAP-positive cells of the four sections were summed and expressed per mm<sup>2</sup>. Glial activation state was rated on a scale ranging from score 0 to 3. The final activation state was calculated as the average of the scores assessed for each of the four representative sections of the trigeminal nucleus caudalis.</p> <p>- Satellite glial cells activation (anti-GFAP, 1:600), calcitonin gene-related peptide (CGRP) immunoreactivity (anti-CGRP, 1:500) and macrophage infiltration (anti-Iba1, 1:500) were evaluated in trigeminal ganglia using immunofluorescence staining. Specifically, trigeminal ganglia were removed and sectioned longitudinally at 20 µm thickness using a cryostat. In the trigeminal ganglia, the number of Iba1+ cells/area and the % of GFAP-encircled neurons and of CGRP+ neurons were assessed in whole TG sections acquired at 20X magnification.</p> <p>- Gene expression analysis: mRNA expression levels of (TNF-alpha), interleukin (IL) -1beta, IL-6, IL-10, inducible nitric oxide synthase (iNOS), GFAP, CD11b and CGRP in medulla oblongata and trigeminal ganglia. mRNA levels were measured by rt-PCR. All samples were assayed in triplicate and gene expression levels were calculated according to 2−∆∆Ct = 2− (∆Ct gene − ∆Ct housekeeping gene) formula by using Ct (cycle threshold) values.</p> <p><strong>Results </strong>- In nitroglycerin-treated rats, ADM_12 showed an anti-hyperalgesic effect in both the acute and chronic models. ADM_12 was able to counteract the acute and chronic nitroglycerin-induced changes in cytokines’ gene expression in both the areas of interest. In the acute nitroglycerin model, we reported a significant increase in microglial and astroglial activation in the trigeminalis nucleus caudalis area, which was abolished by ADM_12 treatment. In the chronic model, we detected an activation of satellite glial cells in the trigeminal ganglia in nitroglycerin-treated rats; this was inhibited by ADM_12. This latter also prevented the increase in CGRP gene expression levels in the trigeminal ganglia of nitroglycerin-treated animals.</p> <p> </p> <p> </p>
Data from: Sex-specific vascular effects of menthol: TRPM8 and TRPA1-dependent relaxation in female mouse aorta and pudendal arteries
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Data from: Evolutionary adaptations of TRPA1 thermosensitivity and skin thermoregulation in vertebrates
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Data from: Comparisons of behavioral and TRPA1 heat sensitivities in three sympatric Cuban Anolis lizards
Thermal tolerances of organisms play a role in defining geographic ranges and occurrence of species. In Cuba, three sympatric species of Anolis lizards (Anolis allogus, Anolis homolechis, and Anolis sagrei) inhabit different thermal microhabitats. A previous study found that these species showed distinct gene expression patterns in response to temperature stimuli, suggesting the genetically distinct thermal physiology among species. To investigate whether the Anolis species inhabiting locally distinct thermal habitats diverge their thermal tolerances, we first conducted behavioral experiments to analyze the temperatures at which the three Anolis species escape from heat source. Then, for each of the three species, we isolated cDNA encoding a putative molecular heat sensor, transient receptor potential ion channel ankyrin 1 (TRPA1), which has been suggested to play a role on eliciting behavioral responses to heat stimuli. We performed electrophysiological analysis to quantify activation temperature of Anolis TRPA1 to see whether the pattern of divergence in TRPA1 responses is congruent with that of divergence in behavioral responses. We found that temperatures triggering behavioral and TRPA1 responses were significantly lower for shade-dwelling species (A. allogus) than for sun-dwelling species (A. homolechis and A. sagrei). The ambient temperature of shade habitats where A. allogus occurs stays relatively cool compared to that of open habitats where A. homolechis and A. sagrei occur and bask. The high temperature thresholds of A. homolechis and A. sagrei may reflect their heat tolerances that would benefit these species to inhabit the open habitats.
Mechanism of Human Cold Pain Perception - Involvement of TRPA1, TRPM8, Nav1.7 and Nav1.8
ClinicalTrials.gov study NCT05935280. IPD Sharing: YES. Countries: 1. Publications: 1.
Mechanisms of Human Heat Perception - Involvement of TRPA1, TRPV1 and TRPM3
ClinicalTrials.gov study NCT05275751. IPD Sharing: YES. Countries: 1. Publications: 0.
Data from: Comparisons of behavioral and TRPA1 heat sensitivities in three sympatric Cuban Anolis lizards
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Data from: Respiratory virus infection up-regulates TRPV1, TRPA1 and ASICS3 receptors on airway cells
Receptors implicated in cough hypersensitivity are transient receptor potential vanilloid 1 (TRPV1), transient receptor potential cation channel, Subfamily A, Member 1 (TRPA1) and acid sensing ion channel receptor 3 (ASIC3). Respiratory viruses, such as respiratory syncytial virus (RSV) and measles virus (MV) may interact directly and/or indirectly with these receptors on sensory nerves and epithelial cells in the airways. We used in vitro models of sensory neurones (SHSY5Y or differentiated IMR-32 cells) and human bronchial epithelium (BEAS-2B cells) as well as primary human bronchial epithelial cells (PBEC) to study the effect of MV and RSV infection on receptor expression. Receptor mRNA and protein levels were examined by qPCR and flow cytometry, respectively, following infection or treatment with UV inactivated virus, virus-induced soluble factors or pelleted virus. Concentrations of a range of cytokines in resultant BEAS-2B and PBEC supernatants were determined by ELISA. Up-regulation of TRPV1, TRPA1 and ASICS3 expression occurred by 12 hours post-infection in each cell type. This was independent of replicating virus, within the same cell, as virus-induced soluble factors alone were sufficient to increase channel expression. IL-8 and IL-6 increased in infected cell supernatants. Antibodies against these factors inhibited TRP receptor up-regulation. Capsazepine treatment inhibited virus induced up-regulation of TRPV1 indicating that these receptors are targets for treating virus-induced cough.
L-menthol as a Topical Counter-irritant to TRPA1-induced Neurogenic Inflammation and Pain
ClinicalTrials.gov study NCT02653703. IPD Sharing: UNDECIDED. Countries: 0. Publications: 8.
Breast/Evaluation of Cryotherapy and TRPA1 Receptors in Chemotherapy Induced Neuropathy
ClinicalTrials.gov study NCT03329131. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Data from: Respiratory virus infection up-regulates TRPV1, TRPA1 and ASICS3 receptors on airway cells
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Differential Methylation Of The TRPA1 Promoter In Pain Sensitivity
GEO Series GSE53130. Homo sapiens. 143 samples. Type: Methylation profiling by high throughput sequencing; Methylation profiling by genome tiling array.
RNAseq of mouse corpus callosum from WT or TRPA1-KO animals under chronic cerebral hypoperfusion
GEO Series GSE201513. Mus musculus. 8 samples. Type: Expression profiling by high throughput sequencing.
Differential Methylation Of The TRPA1 Promoter In Pain Sensitivity: DNA methylation 450k array
GEO Series GSE53128. Homo sapiens. 43 samples. Type: Methylation profiling by genome tiling array.
RNA-seq raw data and analysis for APP, Shal495 and TrpA1 Drosophila melanogaster mutants
GEO Series GSE196770. Drosophila melanogaster. 14 samples. Type: Expression profiling by high throughput sequencing.
Differential Methylation Of The TRPA1 Promoter In Pain Sensitivity: MeDIP-sequencing
GEO Series GSE52965. Homo sapiens. 100 samples. Type: Methylation profiling by high throughput sequencing.
Histone Methyltransferase G9a in Primary Sensory Neurons Promotes Inflammatory Pain and Transcription of Trpa1 and Trpv1 via Bivalent Histone Modifications
GEO Series GSE272517. Mus musculus. 8 samples. Type: Expression profiling by high throughput sequencing.
Effects of 6-month of Treatment With TRPV1 and TRPA1 Agonists in Older Patients With OD
ClinicalTrials.gov study NCT05958173. IPD Sharing: NO. Countries: 1. Publications: 0.
Carnosic acid in topical rosemary extract promotes TRPA1-mediated scarless wound healing
GEO Series GSE298490. Mus musculus. 8 samples. Type: Expression profiling by high throughput sequencing.
Genome-wide Identification of Neuronal Activity-regulated Genes in Drosophila [Elav_TrpA1_ATAC]
GEO Series GSE83975. Drosophila melanogaster. 4 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.