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Figure 7. T in Bioinformatics and expression analysis of the Xeroderma Pigmentosum complementation group C (XPC) of Trypanosoma evansi in Trypanosoma cruzi cells

Figure 7. T. cruzi growth assessment after cisplatin treatment (300 ΜM). (a) Wild type (WT). (b) TcXPC superexpressor (Tc-TcXPC). (c) TevXPC expressor (Tc-TevXPC). The solid lines represent the untreated cells, while the dotted lines represent the cells treated with cisplatin. Statistical student's t test: (*) On that point, cells treated with cisplatin presented a statistically significant lower growth in relation to untreated cells (p <0.05). Representative results of three independent experiments.

Figure 6 in Bioinformatics and expression analysis of the Xeroderma Pigmentosum complementation group C (XPC) of Trypanosoma evansi in Trypanosoma cruzi cells

Figure 6. Growth assessment of T. cruzi: wild type (WT), TcXPC superexpressor (Tc-TcXPC) and TevXPC expressor (Tc-TevXPC). Statistical student's t test: (*) On that point, only Tc-TevXPC presented a statistically significant lower growth in relation to WT (p <0.05); (**) On that point, both Tc-TcXPC and Tc-TevXPC presented a significant lower growth in relation to WT (p <0.05). All parasites were at same initial concentration, grown on LIT medium and were counted daily. Representative results of three independent experiments.

Figure 5 in Bioinformatics and expression analysis of the Xeroderma Pigmentosum complementation group C (XPC) of Trypanosoma evansi in Trypanosoma cruzi cells

Figure 5. TevXPC amplification by RT-PCR with the cDNA from cell cultures. Lanes: (1) 1Kb DNA Ladder; (2) WT; (3) Tc-TcXPC; (4 and 5) Tc-TevXPC; (6) positive control (DNA from T. evansi); (7) negative control.

Figure 4 in Bioinformatics and expression analysis of the Xeroderma Pigmentosum complementation group C (XPC) of Trypanosoma evansi in Trypanosoma cruzi cells

Figure 4. (a) TcXPB-R protein model. (b) TevXPB-R protein model. (c) TcXPB-R (blue) and TevXPB-R (orange) models overlay.

Figure 2 in Bioinformatics and expression analysis of the Xeroderma Pigmentosum complementation group C (XPC) of Trypanosoma evansi in Trypanosoma cruzi cells

Figure 2. (a) Alignment between TcXPC and TevXPC proteins (mismatches highlighted) and its domains. Green: RAD4/PNGase transglutaminase-like fold. Blue: RAD4 beta-hairpin domain 1. Red: RAD4 beta-hairpin domain 2. Yellow: RAD4 beta-hairpin domain 3. (b) Candidate sequence motif involved in p62 interaction (highlighted by brown rectangle) found in TcXPC and TevXPC. This sequence is suggested based on the sequence motif described for Human XPC and yeast RAD4: D/E-F/W-E-D/E-V.

Figure 1 in Bioinformatics and expression analysis of the Xeroderma Pigmentosum complementation group C (XPC) of Trypanosoma evansi in Trypanosoma cruzi cells

Figure 1. (a) TcXPC protein model. (b) TevXPC protein model. (c) TbXPC protein model (d) Model of TevXPC protein bound to a mismatch DNA. (e) TcXPC (red), TevXPC (blue) and TbXPC (green) models overlay. (f) Crystal structure of Rad4-Rad23 bound to a mismatched DNA performed by Min and Pavletich (2007).

Figure 3 in Bioinformatics and expression analysis of the Xeroderma Pigmentosum complementation group C (XPC) of Trypanosoma evansi in Trypanosoma cruzi cells

Figure 3. (a) TcXPB protein model. (b) TevXPB protein model. (c) TcXPB (green) and TevXPB (red) models overlay.

Fig. 4 in Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border

Fig. 4. Path analysis on the influences of contact and feeding on wild mammals in relation to infections of dogs surveyed at Urucum settlement, Corumbá, Mato Grosso do Sul, Brazil in 2015.

Fig. 3 in Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border

Fig. 3. Path analysis on the influences of infections in relation to physical examination of dogs surveyed at Urucum settlement, Corumbá, Mato Grosso do Sul, Brazil in 2015.

Fig. 2 in Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border

Fig. 2. Three-way Venn diagram illustrating coinfection, single infection or no infection of T. cruzi, T. evansi, and Leishmania spp. in 62 dogs from the Urucum settlement along the Brazil-Bolivia border. Total numbers and percentages are presented.

Fig. 1 in Maintenance of Trypanosoma cruzi, T. evansi and Leishmania spp. by domestic dogs and wild mammals in a rural settlement in Brazil-Bolivian border

Fig. 1. The Brazil-Bolivian border and Urucum settlement (Corumbá, MS) demonstrating the site of collections.

Fig. 4 in Selenocysteine in Trypanosoma evansi: Identification of the Genes selb, selc, seld, pstk, seltryp and the Selenophosphate Synthetase Protein

Fig. 4. Detection and cellular localization of the SPS protein, encoded by the selD gene, in T. evansi. A) Detection of 43kDa SPS by western blot T. evansi cellular extracts blotted with an anti-SPS antibody. Lane 1 – Protein Ladder, Lane 2 – T. evansi total protein extract. B) Immunocytochemistry analysis on fixed T. evansi for the determination of SPS extranuclear cellular localization.

Fig. 3 in Selenocysteine in Trypanosoma evansi: Identification of the Genes selb, selc, seld, pstk, seltryp and the Selenophosphate Synthetase Protein

Fig. 3. Secondary structure of T. evansi tRNASec simulated with ARAGORN. Highlighted in red are the anticodon TCA and the elongated extra arm that are characteristic of the specific tRNA for selenocysteine.

Fig. 2 in Selenocysteine in Trypanosoma evansi: Identification of the Genes selb, selc, seld, pstk, seltryp and the Selenophosphate Synthetase Protein

Fig. 2. Molecular phylogenetic analysis by Maximum Likehood method generated in Mega 7 and Plotted in iTOL (Interactive Tree of Life). selD (red), selB (green), PSTK (purple), selTRYP (blue). Protein (gene) codes correspond to those described in Table 2.

Fig. 1 in Selenocysteine in Trypanosoma evansi: Identification of the Genes selb, selc, seld, pstk, seltryp and the Selenophosphate Synthetase Protein

Fig. 1. Detection of gene expression of the selenocysteine pathway in T. evansi. Numbers on the sides indicate molecular size in base pairs. Lanes marked as 1: Molecular-weight size marker (100bp lader Ludwig Biotecnologia- Brazil); Lanes marked as 2: PCR product. selD, selB and selTRYP amplicons ran in 1% agarose gels; selC and PSTK ran in 2% agarose gels. The gene amplified in each reaction is indicated on the bottom.