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16 results for “Yeast Mating”
Fig. 1 in Yeast hydrolysate deprivation and the mating success of male melon flies (Diptera: Tephritidae)
Fig. 1. Relative mating success of treated Z. cucurbitae males in field tent experiments as a function of duration of yeast hydrolysate (YH)-deprivation and cue-lure (CL) exposure, where treated males were cue-lure-deprived in Experiment 1 (solid circles) and cue-lure-fed in Experiment 2 (open circles). Values are means + 1 SE; N = 10 replicates in all cases.
Fig. 2 in Yeast hydrolysate deprivation and the mating success of male melon flies (Diptera: Tephritidae)
Fig. 2. Wing-fanning activity of control and treated males in laboratory cages. Control males were fed yeast hydrolysate (YH) continuously (and deprived of cue-lure [CL]), while treated males were deprived of yeast hydrolysate for 1 or 7 d prior to testing and were either denied or given access to cue-lure 1 d before testing. Bar heights represent average (+ 1 SE) number of males wing-fanning per 1-min check over 45 min preceding sunset on 8 different d (N = 8) for each treatment category.
Data from: Enhanced yeast feeding following mating facilitates control of the invasive fruit pest Drosophila suzukii
The highly invasive spotted wing Drosophila Drosophila suzukii is a key pest of soft fruit and berries in Europe and North America, and development of control techniques is an urgent research challenge. Drosophila suzukii is widely associated with the yeast Hanseniaspora uvarum. Yeasts are symbionts of drosophilid flies and communicate with insects through volatile metabolites for spore dispersal. Accordingly, yeasts and behaviour-modifying chemicals produced by yeasts are prospective tools for environmentally sound insect management. We first bioassayed flight attraction, feeding and oviposition of D. suzukii females in response to H. uvarum yeast and blueberries, which are a preferred host fruit. We then investigated the combined effect of yeast and insecticide on adult female oviposition behaviour and mortality towards the development of a yeast-based control method. Following mating, attraction of female flies to blueberry and yeast odour cues was strongly enhanced. Yeast feeding significantly increased in mated females, while yeast did not increase oviposition on blueberries. This observation suggests that mated flies become attracted to yeast for feeding and to fruit for egg laying. A combined feeding–oviposition assay demonstrated different roles and interference between yeast and fruit stimuli: during the day after mating, females laid fewer eggs when yeast was available. The post-mating yeast-feeding response is an opportunity for the development of an attract-and-kill technique for population control of D. suzukii. Exposing flies to a blend of yeast and insecticide reduced oviposition and greatly enhanced adult fly mortality compared with an insecticide treatment alone. Synthesis and applications. Mated females are the key life stage for Drosophila suzukii population control. Egg-laying females perforate fruit skin and fungal infestations ensue, even when eggs and larvae are killed off by insecticide sprays. Behaviour-modifying chemicals, including yeast metabolites, enable environmentally safe insect management via manipulation of olfactory-mediated reproductive behaviour. Our results highlight that yeast and yeast semiochemicals hold potential for D. suzukii management and that response modulation to olfactory stimuli following mating is a vital element for the development of D. suzukii control methods. Yeast feeding is enhanced in mated D. suzukii females, and this change in post-mating behaviour can be exploited by an attract-and-kill strategy, combining a fly-associated yeast with an insecticide. Furthermore, using the D. suzukii yeast mutualist, H. uvarum, may reduce non-target effects and increase species specificity, which further contributes to the development of an efficient and safe control method.
Data from: Enhanced yeast feeding following mating facilitates control of the invasive fruit pest Drosophila suzukii
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Molecular mechanism of flocculation self-recognition in yeast and its role in mating and survival
Saccharomyces cerevisiae flocculation occurs when fermentable sugars are limiting and is therefore considered as a way to enhance the survival chance of Flo-expressing yeast cells. In this paper the role of Flo1p in mating was demonstrated by showing that the mating efficiency which contributes to the increased survival rate as well by generating genetic variability is increased when cells flocculate. This was revealed by liquid growth experiments in a low shear environment and differential transcriptome analysis of FLO1 expressing cells compared to the non-flocculent wild-type cells. The results show that a floc provides a uniquely organized multicellular ultrastructure that provides a suitable microenvironment to induce and perform cell conjugation. S. cerevisiae strains BY4742 WT BY4742::FLO8 and BY4742 [FLO1] were grown in microgravity and 1-g. A transcriptomic analysis was performed and the transcriptome data were integrated with the high quality protein-protein interaction networks. The identified high score sub-networks (qvalue < 0.001) were considered and further evaluated concerning their GO enrichment using a hypogeometric test. The data were from the Ying B-1 experiment.
Environment-induced same-sex mating suggests homothallism is a primary mode of sexual reproduction in the yeast Candida albicans
GEO Series GSE119165. Candida albicans. 4 samples. Type: Expression profiling by high throughput sequencing.
Barcode Sequencing Screen Identifies SUB1 as a Novel Regulator of Yeast Mating Pathway Genes
GEO Series GSE71813. Saccharomyces cerevisiae. 51 samples. Type: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Other.
The conformation of yeast chromosome III is mating type-dependent and controlled by the recombination enhancer
GEO Series GSE73890. Saccharomyces cerevisiae. 43 samples. Type: Other.
Binding and regulation of transcription by yeast Ste12 variants to drive mating and invasion phenotypes
GEO Series GSE141713. Saccharomyces cerevisiae. 18 samples. Type: Expression profiling by high throughput sequencing; Other.
RNA-sequencing study of global gene expression in haploid yeast Saccharomyces cerevisiae mating type MATa treated with different doses of alpha-pheromone
GEO Series GSE151729. Saccharomyces cerevisiae. 18 samples. Type: Expression profiling by high throughput sequencing.
Regulation of yeast chromosome III architecture and mating-type switching by a Sir2/condensin-bound region of the recombination enhancer [HiC-Seq]
GEO Series GSE92715. Saccharomyces cerevisiae. 10 samples. Type: Other.
Regulation of yeast chromosome III architecture and mating-type switching by a Sir2/condensin-bound region of the recombination enhancer
GEO Series GSE92717. Saccharomyces cerevisiae. 20 samples. Type: Genome binding/occupancy profiling by high throughput sequencing; Other.
Mating response of three yeast species (S. cerevisiae, S. paradoxus and S. mikatae)
GEO Series GSE7525. Saccharomyces cerevisiae; Saccharomyces mikatae; Saccharomyces paradoxus. 13 samples. Type: Expression profiling by array.
Molecular mechanism of flocculation self-recognition in yeast and its role in mating and survival
GEO Series GSE64468. Saccharomyces cerevisiae; Schizosaccharomyces pombe. 17 samples. Type: Expression profiling by array.
Regulation of yeast chromosome III architecture and mating-type switching by a Sir2/condensin-bound region of the recombination enhancer [ChIP-Seq]
GEO Series GSE92714. Saccharomyces cerevisiae. 10 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Molecular mechanism of flocculation self-recognition in yeast and its role in mating and survival
Saccharomyces cerevisiae flocculation occurs when fermentable sugars are limiting and is therefore considered as a way to enhance the survival chance of Flo-expressing yeast cells. In this paper the role of Flo1p in mating was demonstrated by showing that the mating efficiency which contributes to the increased survival rate as well by generating genetic variability is increased when cells flocculate. This was revealed by liquid growth experiments in a low shear environment and differential transcriptome analysis of FLO1 expressing cells compared to the non-flocculent wild-type cells. The results show that a floc provides a uniquely organized multicellular ultrastructure that provides a suitable microenvironment to induce and perform cell conjugation. S. cerevisiae strains BY4742 WT BY4742::FLO8 and BY4742 [FLO1] were grown in microgravity and 1-g. A transcriptomic analysis was performed and the transcriptome data were integrated with the high quality protein-protein interaction networks. The identified high score sub-networks (qvalue < 0.001) were considered and further evaluated concerning their GO enrichment using a hypogeometric test.
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