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3 results for “anatoxin”
Anatoxin concentrations, algal assemblages, and water quality data for the South Fork Eel, Salmon, and Russian Rivers in northern California, 2022-2023
We collected this data to better understand the timing of peak benthic cyanobacterial mat occurrence (specifically taxa associated with anatoxin production, Microcoleus and Anabaena) and mat anatoxin concentrations in rivers. We sampled in northern California on the South Fork Eel, Salmon, and Russian Rivers biweekly in 2022, and the Salmon River biweekly and South Fork Eel weekly in 2023. During each sampling event, we conducted benthic cover surveys, measured in-situ water quality parameters (temperature, pH, dissolved oxygen, conductivity), and collected surface water samples and targeted cyanobacteria samples. In 2022 on all rivers and in 2023 at the Salmon River, we also collected distributed non-targeted periphyton samples to characterize full-reach community compositions. All sampling was completed in 150-m reaches upstream of sensors recording continuous dissolved oxygen, conductivity, and temperature data. We analyzed surface water samples for nitrate, ammonium, soluble reactive phosphate, total dissolved carbon, and dissolved organic carbon. We also analyzed surface water samples from 2022 for major anions (Cl, SO4, Br) and cations (Na, K, Mg, Ca). Targeted-cyanobacteria and non-target periphyton samples were analyzed for anatoxins (and two other classes of toxins, microcystins and cylindrospermopsins), relative abundance of algal taxa (via microscopy), ash-free dry mass, and chlorophyll-a. To estimate mean river depth within the dissolved oxygen footprint upstream of sensors, we kayaked portions of the river and collected river depth measurements. We also measured discharge at each river excluding the Salmon River (due to high discharge) and completed pebble counts at the South Fork Eel River to obtain sediment grain size distributions. Lastly, we estimated daily reach-scale river metabolism (gross primary productivity and ecosystem respiration) using data from dissolved oxygen sensors with the "streamMetabolizer" package in R at all sensor placements.
Figure 2 from: Ilieva V, Kondeva-Burdina M, Georgieva T (2020) In vitro analysis of the activity of human monoamine oxidase type B (hMAOB), treated with the cyanotoxin anatoxin-a: supposed factor of neurodegenerative diseases. Pharmacia 67(2): 111-114. https://doi.org/10.3897/pharmacia.67.e50806
Figure 2 Results from hMAOB activity, treated with anatoxin-a. * P < 0.05; ** P < 0.01; *** P < 0.001 vs control (pure hMAOB).
Figure 1 from: Ilieva V, Kondeva-Burdina M, Georgieva T (2020) In vitro analysis of the activity of human monoamine oxidase type B (hMAOB), treated with the cyanotoxin anatoxin-a: supposed factor of neurodegenerative diseases. Pharmacia 67(2): 111-114. https://doi.org/10.3897/pharmacia.67.e50806
Figure 1 Preparation of the final fluorescent product resorfin (Held 2003).
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