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48 results for “bioassay”
Algal Nutrient Limitation Bioassays in Sycamore Creek, Arizona, USA (2010-2020)
The primary objective of this project is to understand how long-term climate variability and change influence the structure and function of desert streams via effects on hydrologic disturbance regimes. Climate and hydrology are intimately linked in arid landscapes; for this reason, desert streams are particularly well suited for both observing and understanding the consequences of climate variability and directional change. Researchers try to (1) determine how climate variability and change over multiple years influence stream biogeomorphic structure (i.e., prevalence and persistence of wetland and gravel-bed ecosystem states) via their influence on factors that control vegetation biomass, and (2) compare interannual variability in within-year successional patterns in ecosystem processes and community structure of primary producers and consumers of two contrasting reach types (wetland and gravel-bed stream reaches). This dataset addresses patterns of nutrient limitation by primary producers as indicated by *in situ* growth on artificial substrates.
An assessment of the (anti)androgenic properties of hexachloronaphthalene (HxCN) using a model of immature male rats (Hershberger Bioassay)
<p>The persistent organic pollutants (POPs) include polychlorinated naphthalenes (PCNs); of these, the most toxic, abundant and found in human tissues are the hexachloronaphthalenes (HxCNs). The aim of this study was to evaluate the (anti)androgenic action of HxCN using the Hershberger Bioassay (OECD 441). Castrated male Wistar rats were exposed per os to HxCN at daily doses ranging from 0.3-3.0 mg*kg b.w.-1 for 10 days. Testosterone propionate (TP) was used as the reference androgen, and flutamide (FLU) as the reference antiandrogen. Five assessor sex tissues (ASTs) were weighed: ventral prostate, seminal vesicles, levator ani-bulbocavernosus muscle (LABC), glans penis and Cowper gland. In addition to determining the absolute weight of the ASTs, a number of other tests were performed on serum hormone levels (testosterone [T], triiodothyronine 99 [T3], thyroxine [T4], LH and FSH) and the histopathology of the ASTs. </p>
Data for: Evaluation of a full-scale wastewater treatment plant with ozonation and different post-treatments using a broad range of in vitro and in vivo bioassays
<p>This repository contains research data linked to the following publication: Kienle, C., Werner, I., Fischer, S., Lüthi, C., Schifferli, A., Besselink, H., Langer, M., McArdell, C.S. and Vermeirssen, E.L.M. 2022. Evaluation of a full-scale wastewater treatment plant with ozonation and different post-treatments using a broad range of <em>in vitro</em> and <em>in vivo</em> bioassays. Water Research, 118084. https://doi.org/10.1016/j.watres.2022.118084</p> <p>Abstract: Micropollutants present in the effluent of wastewater treatment plants (WWTPs) after biological treatment are largely eliminated by effective advanced technologies such as ozonation. Discharge of contaminants into freshwater ecosystems can thus be minimized, while simultaneously protecting drinking water resources. However, ozonation can lead to reactive and potentially toxic transformation products. To remove these, the Swiss Federal Office for the Environment recommends additional "post-treatment" of ozonated WWTP effluent using sand filtration, but other treatments may be similarly effective. In this study, 48 h composite wastewater samples were collected before and after full-scale ozonation, and after post-treatments (full-scale sand filtration, pilot-scale fresh and pre-loaded granular activated carbon, and fixed and moving beds). Ecotoxicological tests were performed to quantify the changes in water quality following different treatment steps. These included standard <em>in vitro</em> bioassays for the detection of endocrine, genotoxic and mutagenic effects, as well as toxicity to green algae and bacteria, and flow-through <em>in vivo</em> bioassays using oligochaetes and early life stages of rainbow trout.</p> <p>Results show that ozonation reduced a number of ecotoxicological effects of biologically treated wastewater by 66 - 93 %: It improved growth and photosynthesis of green algae, decreased toxicity to luminescent bacteria, reduced concentrations of hormonally active contaminants and significantly changed expression of biomarker genes in rainbow trout liver. Bioassay results showed that ozonation did not produce problematic levels of reaction products overall. Small increases in toxicity observed in a few samples were reduced or eliminated by post-treatments. However, only relatively fresh granular activated carbon (analyzed at 13,000 - 20,000 bed volumes) significantly reduced effects additionally (by up to 66 %) compared to ozonation alone. Inhibition of algal photosynthesis, rainbow trout liver histopathology and biomarker gene expression proved to be sufficiently sensitive endpoints to detect the change in water quality achieved by post-treatment.</p>
Fig.1 in The Use of Ciliates (Ciliophora) for Bioassay of the Toxicity of Insecticides
Fig.1. Changesinthespeciesrichnessofsoilciliatesafterapplicationofinsecticides "ConfidorExtra"(A) and "DecisProfi" (B) (exposure time 7days).
I2i Noise Bioassay Datasets
Open the record for dataset details and reuse information.
Fig. 1 in A "walker" tool to place Diaphorina citri (Hemiptera: Liviidae) adults at predetermined sites for bioassays of behavior in citrus (Sapindales: Rutaceae) trees
Fig. 1. Image of "walker" in use to place Diaphorina citri individual on citrus tree leaf. Inset shows dimensions.
Fig. 3 in Rapid detection of insecticide resistance in Diaphorina citri (Hemiptera: Liviidae) populations, using a bottle bioassay
Fig. 3. Susceptibility of laboratory and field-collected populations of Diaphorina citri to imidacloprid tested at the diagnostic exposure time–concentration combination (A: Lake Alfred 1; B: Winter Garden; C: Lake Alfred 2; D: Frostproof; LB: laboratory strain, FL: Florida field population). Each bar represents mean ± SE. An asterisk (*) indicates significant difference between laboratory and field population at a time period based on a Bonferroni test (P ≤ 0.05).
Fig. 2 in Rapid detection of insecticide resistance in Diaphorina citri (Hemiptera: Liviidae) populations, using a bottle bioassay
Fig. 2. Susceptibility of laboratory and field-collected populations of Diaphornia citri to dimethoate tested at the diagnostic exposure time–concentration combination (A: Lake Alfred 1; B: Winter Garden; C: Lake Alfred 2; D: Frostproof; LB: laboratory strain, FL: Florida field population). Each bar represents mean ± SE. An asterisk (*) indicates significant difference between laboratory and field population based on a Bonferroni test (P ≤ 0.05).
Fig. 1 in Rapid detection of insecticide resistance in Diaphorina citri (Hemiptera: Liviidae) populations, using a bottle bioassay
Fig. 1. Susceptibility of laboratory and field-collected populations of Diaphorina citri of bifenthrin tested at the diagnostic exposure time–concentration combination (A: Lake Alfred 1; B: Winter Garden; C: Lake Alfred 2; D: Frostproof;LB:laboratory strain, FL: Florida field population). Each bar represents mean ± SE.An asterisk (*) indicates significant difference between laboratory and field population at a time period based on a Bonferroni test (P ≤ 0.05).
Fig. 4 in Rapid detection of insecticide resistance in Diaphorina citri (Hemiptera: Liviidae) populations, using a bottle bioassay
Fig. 4. Susceptibility of laboratory and field-collected populations of Diaphorina citri to fenpropathrin tested at the diagnostic exposure time–concentration combination (A: Lake Alfred 1; B: Winter Garden; C: Lake Alfred 2; Frostproof; LB: Laboratory strain, FL: Florida Field strain). Each bar represents mean ± SE. An asterisk (*) indicates significant difference between laboratory and field population at a time period based on a Bonferroni test (P ≤ 0.05).
Fig. 1 in Bioassay of plant extracts against Aleurodicus dispersus (Hemiptera: Aleyrodidae)
Fig. 1. Survival of Aleurodicus dispersus adults exposed to the plant extracts tested by topical spray: 500 mg/L (A), 400 mg/L (B).
Fig. 2 in Sarcocystis falcatula-like derived from opossum in Northeastern Brazil: In vitro propagation in avian cells, molecular characterization and bioassay in birds
Fig. 2. (A) A mature schyzont of Sarcocystis falcatula-like (Sarco-BA1 strain) in a permanent chicken cell line (UMNSAH/DF-1). May-Grüenwald-Giemsa stain. Bar = 20 μm. (B) Extracellular merozoites of Sarco-BA1 on a monolayer of UMNSAH/DF-1 cells. Bar = 10 μm.
Fig. 1 in Sarcocystis falcatula-like derived from opossum in Northeastern Brazil: In vitro propagation in avian cells, molecular characterization and bioassay in birds
Fig. 1. Sporocyst of Sarcocystis falcatula-like. Four sporozoites are visualized inside the sporocyst by light microscopy (A). Autofluorescence of the sporocyst wall is observed after excitation with ultraviolet on a fluorescence microscope (B).
Fig. 1 in A maximum concentration bioassay to assess insecticide efficacy against hemipteran pests of tomato
Fig. 1. Mortality of third instar nymphs from 5 populations of Nezara viridula ('green'), 3 populations of Euschistus quadrator ('brown'), and 1 population of Leptoglossus phyllopus ('leaf') stink bug subjected to a maximum concentration test of 3 pyrethroid and 3 neonicotinoid insecticides and an untreated control (UTC). Percentage mortalities (+ SEM) are based on the mean of 5 nymphs per replicate with 3 replicates. Insecticides designated with the same letter in the horizontal axis are not statistically different (by Bonferroni t-test, P <0.05).
Liriomyza bioassay methodology database
<p>ABSTRACT – <em>Liriomyza sativae</em> infestations cause economic losses of up to 30% in horticulture like melons and tomatoes, usually controlled with insecticides. Despite the high risk of evolution of resistance, rare are the works seeking to establish a standardization in the bioassay methodology that detects <em>L. sativae</em> resistance to insecticides. In this way, this work improved existing bioassay methodologies for larvae of <em>L. sativae</em>, seeking to standardize and make it possible to obtain more consistent data on susceptibility. Two populations of <em>L. sativae</em> were evaluated with insecticides by immersing leaf discs (2 cm in diameter) of melon and pork beans. Treated leaf discs containing a larva were transferred to trays (<em>Bioassay tray</em>) and acrylic plates. Bioassays were evaluated after 48 and 72 hours of exposure. The susceptibility parameters varied with exposure, type of arena and host, as well as the mode of action of the insecticides. Bioassays in the <em>Bioassay tray</em> provided greater homogeneity of response compared to acrylic plates. The response concentration curves were more accurate and homogeneous with melon leaves compared to pig bean leaves, presenting LC values well below these latter. Although the two methodologies can be used for toxicological bioassays, however, the use of <em>Bioassay tray</em> showed less economic cost and greater agility in the elaboration. Therefore, the enhancement toxicological bioassay methodology is suggested for <em>Liriomyza</em> species that provides more reproducible and less heterogeneous results, enabling research on susceptibility and monitoring of resistance to insecticides.</p> <p> </p>
Reproductives signature revealed by protein profiling and behavioral bioassays in termite
<p>Proteins are known to be social interaction signals in many species in the animal kingdom. The aim of this project is to explore the potential role of cuticular polar compounds in reproductive recognition on termites. Cuticular polar fractions were extracted from reproductives and workers. These extracts were used for molecular profiling, top-down proteomics and tested in behavioral bioassays to measure behavioral response in termite.</p>
Following the mixtures of organic micropollutants with in-vitro bioassays in a large lowland river from source to sea - bioassays CRC
<p>Supportive material for the submitted paper: </p> <p><strong><em><span>Following the mixtures of organic micropollutants with in-vitro bioassays in a large lowland river from source to sea </span></em></strong></p> <p><span>from Hommel et al.</span></p> <p><span>The data includes the with R automated evaluation of the AhR-CALUX, AREc32, ERa-GeneBLAzer and SH-SY5Y assay with respective plots and excel files of the concentrations response curves.</span></p>
Laboratory bioassay of insecticides mixtures (neonicotinoids and ketoenols) against Bemisia tabaci Asia I
<p>Cotton leaves were dipped in serially diluted solutions of formulated insecticides for 10 s with slight agitation. The leaves with second instar nymphs that were dipped in double-distilled water containing 0.1 g L<sup>-1</sup> Triton X-100 only, served as control. Each bioassay including control used 3-4 replicates at a minimum of eight different concentrations and were maintained at the controlled growth condition. All the insecticides concentrations were selected to give a range of 0-100% mortality of <em>B. tabaci</em> nymphs. Two weeks later final mortality was assessed when the last nymphal instar had been reached on control plants. It was computed by comparing the number of second instar nymphs present at the time of treatment with the number remaining dead or unhatched on the day of mortality assessment. For bioassays with synergists (PBO and DEF), the cotton leaves containing the second instar nymphs of <em>B. tabaci</em> were dipped into synergist solutions (100 mg L<sup>-1</sup>) for 10 s at least 2 h before the imposition of insecticide treatments. Other procedures were same to those with insecticides only.</p>
Dataset of bioassays about resource selection in N. corniger
<p>Dataset of bioassays conducted with <em>Nasutitermes corniger</em> to test the effect of food resource and alarm signal on resource selesction. </p>
Dataset of ocean nutrient addition bioassay experiments
<p>Dataset of ocean nutrient addition bioassay experiments. Accompanies publication: 'Global analysis of ocean phytoplankton nutrient limitation reveals high prevalence of co-limitation' by Thomas J. Browning and C. Mark Moore.</p>
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.