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95 results for “biosensors”
Detecting Changes in the Caenorhabditis elegans Intestinal Environment Using an Engineered Bacterial Biosensor
<p>Data for the figures in the manuscript <br> <a href="https://pubs.acs.org/doi/10.1021/acssynbio.9b00166">https://pubs.acs.org/doi/10.1021/acssynbio.9b00166</a></p> <p>Abstract:<br> <em>Caenorhabditis elegans</em> has become a key model organism within biology. In particular, the transparent gut, rapid growing time, and ability to create a defined gut microbiota make it an ideal candidate organism for understanding and engineering the host microbiota. Here we present the development of an experimental model that can be used to characterize whole-cell bacterial biosensors <em>in vivo</em>. A dual-plasmid sensor system responding to isopropyl β-d-1-thiogalactopyranoside was developed and fully characterized <em>in vitro</em>. Subsequently, we show that the sensor was capable of detecting and reporting on changes in the intestinal environment of <em>C. elegans</em> after introducing an exogenous inducer into the environment. The protocols presented here may be used to aid the rational design of engineered bacterial circuits, primarily for diagnostic applications. In addition, the model system may serve to reduce the use of current animal models and aid in the exploration of complex questions within general nematode and host–microbe biology.</p>
DNA origami book biosensor for multiplex detection of cancer-associated nucleic acids
<p>This dataset contains the raw data that were used for the publication entitled, "DNA origami book biosensor for multiplex detection of cancer-associated nucleic acids" published in Nanoscale.</p> <p> </p> <p>Abstract</p> <p>DNA nanotechnology provides a promising approach for the development of biomedical point-of-care diagnostic nanoscale devices that are easy to use and cost-effective, highly sensitive and thus constitute an alternative to expensive, complex diagnostic devices. Moreover, DNA nanotechnology-based devices are particularly advantageous for applications in oncology, owing to being ideally suited for the detection of cancer-associated nucleic acids, including circulating tumor-derived DNA fragments (ctDNAs), circulating microRNAs (miRNAs) and other RNA species. Here, we present a dynamic DNA origami book biosensor that is precisely decorated with arrays of fluorophores acting as donors and acceptors and also fluorescence quenchers that produce a strong optical readout upon exposure to external stimuli for the single or dual detection of target oligonucleotides and miRNAs. This biosensor allowed the detection of target molecules either through the decrease of Förster resonance energy transfer (FRET) or an increase in the fluorescence intensity profile owing to a rotation of the constituent top layer of the structure. Single-DNA origami experiments showed that detection of two targets can be achieved simultaneously within 10 min with a limit of detection in the range of 1–10 pM. Overall, our DNA origami book biosensor design showed sensitive and specific detection of synthetic target oligonucleotides and natural miRNAs extracted from cancer cells. Based on these results, we foresee that our DNA origami biosensor may be developed into a cost-effective point-of-care diagnostic strategy for the specific and sensitive detection of a variety of DNAs and RNAs, such as ctDNAs, miRNAs, mRNAs, and viral DNA/RNAs in human samples.</p>
A DNA biosensors-based microfluidic platform for attomolar real-time detection of unamplified SARS-CoV-2 virus
<p>Raw data associated to the study entitled:</p> <p><em>A DNA biosensors-based microfluidic platform for attomolar real-time detection of unamplified SARS-CoV-2 virus</em><strong> </strong></p> <p><em>- </em>Metadata file</p> <p>- Computational data</p> <p>- Extraction data</p> <p>- Fluorescence detection</p> <p>- Fluorescence imaging</p> <p>- Labbooks</p> <p>- Surface characterization</p>
A FRET based biosensor for measuring Gα13 activation in single cells
<p>The figures and raw data that are presented in the paper "A FRET based biosensor for measuring Gα13 activation in single cells"</p>
Grafting-from" and "grafting-to" PNIPAM functionalization of glass for DNA-biosensors with improved properties
<p>Raw data and metadata for the study “Grafting-from” and “grafting-to” PNIPAM functionalization of glass for DNA-biosensors with improved properties"</p> <ul> <li>Copy of labbooks for synthesis and surface functionalization protocols</li> <li>Molecular characterization: RMN, MS, GPC, LCST</li> <li>Surface characterization : water contact angle, surface quantification, XPS, antifouling properties</li> </ul>
Comparison of heterologous β-alanine-responsive biosensors in Escherichia coli
<p>This dataset contains the raw data that lie at the basis of the results discussed in <strong>Chapter 6: Comparison of heterologous β-alanine-responsive biosensors in <em>Escherichia coli</em> </strong>of the PhD thesis of Amber Bernauw. The README.txt file provides more information on the different data files.</p>
Alcohol Biosensor Monitoring for Alcoholic Liver Disease
ClinicalTrials.gov study NCT03533660. IPD Sharing: YES. Countries: 1. Publications: 1.
Whole-cell Escherichia coli lactate biosensor for monitoring mammalian cell cultures during biopharmaceutical production-- dataset
<p>Raw data for Goers et al, (2017), Biotech Bioeng. doi:10.1002/bit.26254.</p> <p>Each graph including the supplementary figures is a separate tab.</p>
Label-free multiplexed detection of diabetic retinopathy biomarkers using fiber optic biosensors: towards lab-in-the-tear
<p>Raw experimental data on label-free detection of diabetic retinopathy biomarkers using fiber optic biosensors. This data contains information on the multiplexed and separate detection of LCN1 and VEGF diabetic retinopathy biomarkers in artificial tears. </p>
Surface Plasmon Resonance Biosensor with Anti-Crossing Modulation Readout
Open the record for dataset details and reuse information.
Biosensor Dataset: Supporting research on biosensor technology for overall water quality monitoring
<p>Dataset regarding vision-based and MEMS-based biosensor technology validation within ASTRAL IMTA labs.</p>
Physical Insights from Frumkin Isotherm Applied to Electrolyte Gated Organic Transistor as Protein Biosensors
<p>The uploaded data include the transfer curves at every IL-6 concentration and for control experiments (TNFalpha and IL-1beta).</p> <p>Electrical measurements were acquired in 50 mM PBS, pH 7.4, containing a constant concentration of 0.1 mg/mL BSA and 0.05% Tween 20 under static conditions.</p> <p>Anti-IL-6 antibodies were immobilized on the gate electrode through EDC/NHS coupling onto mixed mercaptoundecanoic acid:6-mercaptohexanol (1:3) SAM.</p>
Interactive computational and experimental approaches improve the sensitivity of periplasmic binding protein-based nicotine biosensors for measurements in biofluids
<p>Here are the raw simulation trajectories (water removed).</p>
Mode characterization and sensitivity evaluation of an ultra-high-frequency surface acoustic wave (UHF-SAW) resonator biosensor: application to the glial-fibrillary-acidic-protein (GFAP) biomarker detection
<p>Biosensors detect specific bio-analytes by generating a measurable signal from the interaction between the sensing element and the target molecule. Surface acoustic wave (SAW) biosensors offer unique advantages due to their high sensitivity, real-time response capability, and label-free detection. The typical SAW modes are the Rayleigh mode and the shear-horizontal mode. Both present pros and cons for biosensing applications and generally need different substrates and device geometries to be efficiently generated. This study investigates and characterizes ultra-high-frequency (UHF-) SAW resonator biosensors. It reveals the simultaneous presence of the two typical SAW modes, clearly separated in frequency, called slow and fast. The two modes are studied by numerical simulations and biosensing experiments with the glial-fibrillary-acidic-protein (GFAP) biomarker. The slow mode is generally more sensitive to changes in surface properties, such as temperature and mass changes, by a factor of about 1.4 with respect to the fast mode.</p>
Chromophore carbonyl twisting in fluorescent biosensors encodes direct readout of protein conformations with multicolor switching
<p>Fluorescent labeling of proteins is a powerful tool for probing structure-function relationships with many biosensing applications. Structure-based rules for systematically designing fluorescent biosensors require understanding ligand-mediated fluorescent response mechanisms which can be challenging to establish. We installed thiol-reactive derivatives of the naphthalene-based fluorophore Prodan into bacterial periplasmic glucose-binding proteins. Glucose binding elicited paired color exchanges in the excited and ground states of these conjugates. X-ray structures and mutagenesis studies established that glucose-mediated color switching arises from steric interactions that couple protein conformational changes to twisting of the Prodan carbonyl relative to its naphthalene plane. Mutations of residues contacting the carbonyl can optimize color switching by altering fluorophore conformational equilibria in the apo and glucose-bound proteins. A commonly accepted view is that Prodan derivatives report on protein conformations via solvatochromic effects due to changes in the dielectric of their local environment. Here we show that instead Prodan carbonyl twisting controls color switching. These insights enable structure-based biosensor design by coupling ligand-mediated protein conformational changes to internal chromophore twists through specific steric interactions between fluorophore and protein.</p>
Tailoring the hybridization density of DNA biosensors through tunable surface functionalization
<p>Raw data and metadata associated to the study "Tailoring the hybridization density of DNA biosensors through tunable surface functionalization"</p> <p>Metadata file.</p> <p>Raw data for molecular characterization (NMR and MS analyses).</p> <p>Raw data for surface characterization (XPS analyses and fluorescence quantification).</p> <p>Copy of labbook for synthesis and surface functionalization protocols.</p> <p> </p>
Project ADHERE: Clinical Proof-of-Concept of a Tenofovir (TFV) Aptamer-Based Biosensor
ClinicalTrials.gov study NCT04870671. IPD Sharing: NO. Countries: 1. Publications: 3.
Project Engage: A Wrist Biosensor-based mHealth Suite to Support Alcohol Intervention in Young People Living With HIV
ClinicalTrials.gov study NCT05431855. IPD Sharing: UNDECIDED. Countries: 1. Publications: 1.
Development of Ingestible Biosensors to Enhance PrEP Adherence in Substance Users (PrEPSteps)
ClinicalTrials.gov study NCT03512418. IPD Sharing: UNDECIDED. Countries: 1. Publications: 1.
Chromophore carbonyl twisting in fluorescent biosensors encodes direct readout of protein conformations with multicolor switching
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.