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801 results for “buds”
Eight Mile Lake Research Watershed, Carbon in Permafrost Experimental Heating Research (CiPEHR): Phenology of Dominant Plant Species I - Bud burst and Senescence 2013-2021
The Carbon in Permafrost Experimental Heating Research (CiPEHR) project addresses the following questions: 1) Does ecosystem warming cause a net release of C from the ecosystem to the atmosphere?, 2) Does the decomposition of old C, that comprises the bulk of the soil C pool, influence ecosystem C loss?, and 3) How do winter and summer warming alone, and in combination, affect ecosystem C exchange? We are answering these questions using a combination of field and laboratory experiments to measure ecosystem carbon balance and radiocarbon isotope ratios at a warming experiment located in an upland tundra field site near Healy, Alaska in the foothills of the Alaska Range. In this dataset we specifically ask, how does warming of soil and air impact the phenology of dominant plant species? Phenological data was collected to determine the timing of first bud break, onset and completion of senescence, and reproductive effort (flower and berry production).
Eight Mile Lake Research Watershed, Carbon in Permafrost Experimental Heating and Drying Research (DryPEHR): Phenology of Dominant Plant Species I - Bud burst and Senescence 2013-2021
This drying and warming experiment addresses the following questions: 1) Does ecosystem drying, warming and permafrost thaw cause a net release or uptake of C from the ecosystem to the atmosphere?, 2) Does the decomposition of old C that comprises the bulk of the soil C pool influence ecosystem C loss? 3) How do drying and warming affect plant communities and ecosystem properties? We are answering these questions using a combined warming and drying experiment (DryPEHR), which is situated with the Carbon in Permafrost Experimental Heating Research (CiPEHR) project and located in an upland tundra field site near Healy, Alaska in the foothills of the Alaska Range. Warming treatment here refers to growing season air temperature warming (~1C) using open top chambers (OTC) combined with soil 'warming' using snow fences during the snow covered months. Drying is achieved using an automated pumping system that lowers the water table in the dry plots. Soil warming began in 2008; OTCs and drying in 2011. In this dataset we specifically ask, how does warming and drying of soil and air impact the phenology of dominant plant species? Phenological data was collected to determine the timing of first bud break, onset and completion of senescence, and reproductive effort (flower and berry production).
Developmental stages of bud primordia in Vaccinium vitis-idaea across 16 sites in Interior Alaska 2017-2019
This dataset contains the stages of the flower bud primordia (buds prior to anthesis) for Vaccinium vitis-idaea at 19 sites in the Fairbanks area for two cohorts (one initiated in 2017 and matured in 2018, and one initated in 2018 and matured in 2019). Developmental stages were tracked from early June to early October, and then again the following May. Buds were classified as basal, middle, or apical. Developmental stages are based on scanning lectron microscopy. Associated data describe temperature for each interval (period between collection dates), along with site characteristics (location, canopy cover, and depth of thaw in late May).
Fig. 7. Begonia acetosella Craib. A. Plant habit. B – C. Leaf variation. D. Female bud. E. Female flower. F in A revision and one new species of Begonia L. (Begoniaceae, Cucurbitales) in Northeast India
Fig. 7. Begonia acetosella Craib. A. Plant habit. B – C. Leaf variation. D. Female bud. E. Female flower. F. Reverse of flower. G. Styles. Photographs by Rebecca Camfield of a plant in cultivation at the Royal Botanic Garden Edinburgh (accession 19980065).
Supplementary Material: Fluorescent Protein‐Tagged Sindbis Virus E2 Glycoprotein Allows Single Particle Analysis of Virus Budding from Live Cells
<p>Supplementary Videos for <em>Viruses</em> <strong>2015</strong>, <em>7</em>(12), 6182-6199; doi:10.3390/v7122926, http://www.mdpi.com/1999-4915/7/12/2926:</p> <p><strong>Video S1A</strong> BHK cells infected with mCherry-E2 virus at 3 h p.i. Glycoprotein containing vesicles are transported to the PM from where individual virions bud out. White arrow point to budding virions. Overall amount of glycoproteins present on the PM and the number of virus particles budding out are relatively reduced compared to the late stage of infection. Images were acquired at a rate of 0.99 fps and 75 frames were acquired. Video was generated using these images and played at a rate of 5 fps. Image acquisition time is shown as Time: hour: minute: second: millisecond (h:min:sec:msec ) and the scale bar represents 10 μm.</p> <p><strong>Video S1B </strong>Enlarged area of video S1A showing budding virus particles from PM. White arrow indicates single particle post-budding moving away from the cell. Images were acquired at a rate of 0.99 fps and 75 frames were acquired. Video was generated using these images and played at a rate of 5 fps. Image acquisition time is shown as Time: hour: minute: second: millisecond (h:min:sec:msec ) and the scale bar represents 10 μm.</p> <p><strong>Video S2A</strong> Virus budding and single particle movement associated with filopodial extensions observed from mCherry-E2 virus-infected BHK cells at 6 h p.i. Glycoprotein containing vesicle transport to the PM is also observed. Budded virions travel along the periphery of filopodia and are released from filopodial extensions to the surrounding media. Image acquisition was at a rate of 1 fps and 285 frames were acquired. Movie was generated using these images and played at a rate of 7 fps. Image acquisition time is shown as Time: h:min:sec:msec and the scale bar represents 10 μm.</p> <p><strong>Video S2B</strong> Enlarged area of video S2A showing budding virus particles from filopodia. White arrow indicates virus budding from filopodial extensions. Images were acquired at a rate of 1 fps and the acquired 285 frames were used to generate the video at a rate of 7 fps. Image acquisition time is shown as Time: h:min:sec:msec and the scale bar represents 10 μm.</p> <p><strong>Video S3</strong> BHK cells transfected with RNA from a non-budding cdE2 mutant <sub>416</sub>CC<sub>417</sub>/A2 mCherry-E2 virus. This non-budding mutant is unable to release fluorescent virus particles from the infected cells. The video shows the absence of fluorescent virus particle budding from the PM at 6 h post transfection even though the PM and filopodial extensions contain mCherry-E2. Despite the transport of glycoproteins to the PM, no fluorescent particles were released into the media. Yellow arrows point toward filopodial extensions. For the video, 304 images were acquired at a rate of 0.98 fps and the video was generated using the acquired images at a rate of 7 fps. Image acquisition time is shown as Time: h:min:sec:msec and the scale bar represents 10 μm.</p> <p><strong>Video S4</strong> BHK cells transfected with RNA from an E1 Fusion loop (G91D) mutant of mCherry-E2 virus at 6 h post transfection. This non-fusing mutant produces fluorescent virus particles at a slower rate compared to WT that are unable to fuse after entering a new cell. White arrow points to fluorescent particles that are releasing into the media from filopodial extensions. Yellow arrow represents a fluorescent particle that had entered an adjacent un-transfected cell. A total of 149 images were acquired at a rate of 0.98 fps. Video was generated using these images at a rate of 7 fps. Image acquisition time is shown as Time: h:min:sec:msec and the scale bar represents 10 μm.</p> <p> </p> <p><strong>Video S5A</strong> Glycoprotein E2 (mCherry-E2; red) colocalizing with Golgi stain (green) in BHK cells infected with mCherry-E2 virus and stained with BODIPY FL C5 ceramide at 5 h p.i. and imaged at 6 h p.i. Glycoprotein-containing red vesicles originate from Golgi as evidenced from the colocalization of red and green and these vesicles display anterograde transport to the PM and the virus particles are released by budding from the PM. Fluorescent particles are also seen budding from filopodial extensions (white arrows). Images were acquired at a rate of 0.13 fps for 295 seconds. Video was generated using these acquired images at a rate of 5 fps. Image acquisition time is shown as Time: h:min:sec:msec and the scale bar represents 10 μm.</p> <p><strong>Video S5B</strong> An enlarged area of the video S5A near the white arrow showing movement of particles on filopodial extensions between two cells. Movie was played at a rate of 5 fps. Image acquisition time is shown as Time: h:min:sec:msec and the scale bar represents 10 μm.</p> <p> </p> <p> </p>
Fig. 6. Homalia trichomanoides gametophore bud development. Day 148 in Preliminary Study Of Moss Homalia Trichomanoides (Hedw.) Brid. Gametophyte Development From Spores In Vitro
Fig. 6. Homalia trichomanoides gametophore bud development. Day 148 after inoculation (A) and day 158 after inoculation (B).
Data from: Transcriptome analysis of apical meristem enriched bud samples for size dependent flowering commitment in Crocus sativus reveal role of sugar and auxin signalling
<p><strong>Background</strong></p> <p>Cultivation of <em>Crocus sativus</em> (saffron) faces challenges due to inconsistent flowering patterns and variations in yield. Flowering takes place in a graded way with smaller corms unable to produce flowers. Enhancing the productivity requires a comprehensive understanding of the underlying genetic mechanisms that govern this size based flowering initiation and commitment. Therefore, samples enriched with non-flowering and flowering apical buds from small (<6g) and large (>14g) corms were sequenced. </p> <p><strong>Methods and Results</strong></p> <p>Apical bud enriched samples from small and large corms were collected immediately after break of dormancy in July. RNA sequencing was performed using Illumina Novaseq 6000. <em>De-novo</em> transcriptome assembly and analysis using flowering committed buds from large corms at post-dormancy and their comparison with vegetative shoot primordia from small corms pointed out the major role of Auxin and ABA hormonal regulation. Many genes with known dual responses in flowering development and circadian rhythm like Flowering locus T and Cryptochrome 1 along with a transcript showing homology with small auxin upregulated RNA (SAUR) exhibited induced expression in flowering buds. Thorough prediction of <em>Crocus sativus</em> non-coding RNA repertoire has been carried out for the first time. Enolase was found to be acting as a major hub with protein-protein interaction analysis using Arabidopsis counterparts.</p> <p><strong>Conclusion</strong></p> <p>Transcripts belong to key pathways including phenylpropanoid biosynthesis, hormone signaling and carbon metabolism were found significantly modulated. KEGG assessment and protein-protein interaction analysis confirm the expression data. Findings unravel the genetic determinants driving the size-dependent flowering in <em>Crocus sativus</em>.</p>
Quercus falcata (Fagaceae) - twig - close-up winter terminal bud
Image of Quercus falcata (Fagaceae) - twig - close-up winter terminal bud
Rhododendron catawbiense (Ericaceae) - twig - close-up winter terminal bud
Image of Rhododendron catawbiense (Ericaceae) - twig - close-up winter terminal bud
Amelanchier arborea (Rosaceae) - twig - close-up winter terminal bud
Image of Amelanchier arborea (Rosaceae) - twig - close-up winter terminal bud
Cornus sericea (Cornaceae) - twig - close-up winter terminal bud
Image of Cornus sericea (Cornaceae) - twig - close-up winter terminal bud
Malus pumila (Rosaceae) - twig - close-up winter terminal bud
Image of Malus pumila (Rosaceae) - twig - close-up winter terminal bud
Carya cordiformis (Juglandaceae) - twig - close-up winter terminal bud
Image of Carya cordiformis (Juglandaceae) - twig - close-up winter terminal bud
Magnolia macrophylla (Magnoliaceae) - twig - close-up winter terminal bud
Image of Magnolia macrophylla (Magnoliaceae) - twig - close-up winter terminal bud
Cornus florida (Cornaceae) - twig - close-up winter terminal bud
Image of Cornus florida (Cornaceae) - twig - close-up winter terminal bud
Tilia americana var. heterophylla (Tiliaceae) - twig - close-up winter terminal bud
Image of Tilia americana var. heterophylla (Tiliaceae) - twig - close-up winter terminal bud
Quercus coccinea (Fagaceae) - twig - close-up winter terminal bud
Image of Quercus coccinea (Fagaceae) - twig - close-up winter terminal bud
Quercus shumardii (Fagaceae) - twig - close-up winter terminal bud
Image of Quercus shumardii (Fagaceae) - twig - close-up winter terminal bud
Cornus florida (Cornaceae) - twig - close-up winter terminal bud
Image of Cornus florida (Cornaceae) - twig - close-up winter terminal bud
Cornus florida (Cornaceae) - twig - close-up winter terminal bud
Image of Cornus florida (Cornaceae) - twig - close-up winter terminal bud
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Allen Brain Atlas
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Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
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The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
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