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73 results for “cell viability”
DS4_LH_ Lodola et al_Sci Adv_2019_Cell Viability
<p>Cell Viability and tubulogenesis assays in dark and light conditions. </p>
DS4_LH_Tullii et al._ACS Appl. Mater. Interfaces_2019_cell viability
<p>cell viability assay on HEK cells and neurons plated on P3HT flat and pillars</p>
Silver Nanoparticles Alter Cell Viability Ex Vivo and in Vitro and Induce Proinflammatory Effects in Human Lung Fibroblasts
<p>Dataset for data generated and presented in following article by Löfdahl et al in Nanomaterials 2020, 10, 1868. doi:10.3390/nano10091868</p>
Underlying data of Impact of shipping temperature on cell viability and T cell responses to bacterial antigens
<p><strong>Abstract</strong></p> <p><strong>Background: </strong>Interferon-γ (IFN-γ) secretion by T cells is a key correlate of immune protection against many pathogens including tuberculosis and the neglected tropical disease melioidosis. Clinical studies in tropical regions of immune responses to pathogens and vaccine monitoring studies require the collection of samples in resource-limited rural areas and subsequent shipment to central laboratories for downstream assays and long-term storage. Here, we studied the impact of two different shipping temperatures on the viability, composition and function of peripheral blood mononuclear cells (PBMC) using multi-colour flow cytometry and IFN-g enzyme-linked immunospot assay (IFN-g ELISpot), in order to provide guidance on sample shipment conditions for future clinical studies.</p> <p><strong>Methods</strong>: Paired peripheral blood mononuclear cell (PBMC) samples from recovered melioidosis patients were stored in liquid nitrogen (-196°C) and then shipped from Bangkok, Thailand to Oxford, UK at either -80°C (dry ice) or -196°C (dry shipper). After thawing, cell viability and composition were assessed by flow cytometry and antigen specific responses to <em>Burkholderia pseudomallei</em> (BP) were measured using IFN-g ELISpot.</p> <p><strong>Results</strong>: We observed modest lowering of viability in the majority of samples and a reduction in IFN-g responses to BP which correlated to a decrease of monocytes and NK cells in samples shipped at -80°C compared to -196°C. Despite being lower in magnitude antigen-specific responses remained detectable in the majority of samples.</p> <p><strong>Conclusions:</strong>Here we demonstrate that shipment of cryopreserved PBMC at -196°C has a benefit on cell viability, recovery and T cell responses to bacterial antigens, although useful information can still be obtained from samples shipped at -80°C, thus providing important guidance for sample management in future clinical trials.</p>
Supplemental information for: Inhibition of CSF1R and KIT with pexidartinib reduces inflammatory signaling and cell viability in endometriosis
<p>Endometriosis is a common and debilitating disease, affecting ~170 million women worldwide. Affected patients have limited therapeutic options such as hormonal suppression or surgical excision of the lesions, though therapies are often not completely curative. Targeting receptor tyrosine-kinases (RTKs) could provide a nonhormonal treatment option for endometriosis. We determined that two RTKs, Macrophage colony stimulating factor receptor (CSF1R) and Mast/stem cell growth factor receptor KIT (KIT), are overexpressed in endometriotic lesions and could be novel nonhormonal therapeutic targets for endometriosis. The kinase activity of CSF1R and KIT is suppressed by pexidartinib, a small molecule inhibitor that was recently approved by the US Food and Drug Administration (FDA). Using immunohistochemistry, we detected CSF1R and KIT in endometriotic tissues obtained from peritoneal lesions, colorectal lesions, and endometriomas. Specifically, we show that KIT is localized to the epithelium of the lesions, while CSF1R is expressed in the stroma and macrophages of the endometriotic lesions. Given the high epithelial expression of CSF1R and KIT, 12Z endometriotic epithelial cells were used to evaluate the efficacy of dual CSF1R and KIT inhibition with pexidartinib. We found that pexidartinib suppressed activation in 12Z cells of JNK, STAT3 and AKT signaling pathways, which control key pro-inflammatory and survival networks within the cell. Using quantitative real time PCR, we determined that pexidartinib suppressed interleukin 8 (<em>IL8) </em>and cyclin D1 (<em>CCND1) </em>expression<em>.</em> Lastly, we demonstrated that pexidartinib decreased cell growth and viability.<em> </em>Overall, these results indicate that pexidartinib-mediated CSF1R and KIT inhibition reduces pro-inflammatory signaling and cell viability in endometriosis.</p>
Opt2Q Example cell_viability_calibration_results
<p>A model of apoptosis (aEARM) was calibrated to published cell viability data using PyDREAM. The posterior sample for the model parameters and log-posterior traces are included as .npy files. The .txt files show the Gelman Rubin metric for each of the model parameters. The data was generated using https://github.com/LoLab-VU/aEARM_cell_viability_calibration</p>
Optimisation of viability assay for DIPG patient-derived cell lines
<p>Evaluation of the efficacy of M4K compounds in DIPG patient-derived cell lines is essential before any promising compounds can be further tested in mouse xenograft models. This approach can aid in narrowing down clinical compound candidates and reduce the time, resources and animal sacrifice needed downstream.</p> <p>A robust and efficient readout for the changes in the viability of the DIPG cells needs to be established before it can be used to evaluate the M4K compounds. In addition, the amount of cells to be seeded at the beginning of the experiment has to be optimised to avoid overcrowding and starvation of the cells after extended culture times. Overcrowding and starvation will lead to increased cell death and prevent accurate estimation of the potency of M4K compounds (EC50).</p>
Evaluating the effect of 8 M4K compounds on the viability of DIPG patient-derived cells (SU-DIPG-VI, HSJD-DIPG-007 and SU-DIPG-IV)
<p>Viability assay using Celltiter Glo kit has been chosen as the main method for evaluating the efficacy of ALK2 inhibitors on DIPG patient-derived cells due to its robustness. As a pilot experiment, 8 ALK2 inhibitors with differing potencies were chosen and evaluated in 3 DIPG patient-derived cell lines (one with wild-type ALK2 and two with mutant ALK2).</p>
Data from: Antineoplastic Nature of WWOX in Glioblastoma Is Mainly a Consequence of Reduced Cell Viability and Invasion
<p>Supporting data for the article "Antineoplastic Nature of WWOX in Glioblastoma Is Mainly a Consequence of Reduced Cell Viability and Invasion ", published in Biology (DOI: <span>10.3390/biology12030465</span>).</p>
Supplemental information for: Inhibition of CSF1R and KIT with pexidartinib reduces inflammatory signaling and cell viability in endometriosis
Open the record for dataset details and reuse information.
Developing and Study of on-line Biotoxicity Sensing and Control Unit for Wastewater Treatment, based on a Cell Viability Monitoring Assay
<p>The quality of industrial, municipal or hospital waters varies greatly throughout the days and seasons. Because usually one of the first steps of water treatment plants (WWTPs) is biological treatment (after mechanical cleaning) it is vital that this step operates at full capacity. However, toxic compounds such as antibiotics, which are in use for human consumption or animal agriculture can greatly disrupt this stage of cleaning due to their antibiological nature of operation.</p> <p>Technion has developed a system in which toxicity to bacteria is measured on-line. It is based on following spectral changes in a dye, which is reduced as part of bacterial metabolism, which depends on the level of toxicity. The measured toxicity values are fed to a PID controller, which controls the operation of an AOP reactor.</p> <p>This system was developed as part of "Project O", a H2020 program of the European Commission.</p>
The Viability Control of Human Endothelial Cells Before Keratoplasty (V-CHECK) Study
ClinicalTrials.gov study NCT05847387. IPD Sharing: YES. Countries: 6. Publications: 1.
Data from: Impact of genetic reduction of NMNAT2 on chemotherapy-induced losses in cell viability in vitro and peripheral neuropathy in vivo
Nicotinamide mononucleotide adenylyl transferases (NMNATs) are essential neuronal maintenance factors postulated to preserve neuronal function and protect against axonal degeneration in various neurodegenerative disease states. We used in vitro and in vivo approaches to assess the impact of NMNAT2 reduction on cellular and physiological functions induced by treatment with a vinca alkaloid (vincristine) and a taxane-based (paclitaxel) chemotherapeutic agent. NMNAT2 null (NMNAT2-/-) mutant mice die at birth and cannot be used to probe functions of NMNAT2 in adult animals. Nonetheless, primary cortical cultures derived from NMNAT2-/- embryos showed reduced cell viability in response to either vincristine or paclitaxel treatment whereas those derived from NMNAT2 heterozygous (NMNAT2+/-) mice were preferentially sensitive to vincristine-induced degeneration. Adult NMNAT2+/- mice, which survive to adulthood, exhibited a 50% reduction of NMNAT2 protein levels in dorsal root ganglia relative to wildtype (WT) mice with no change in levels of other NMNAT isoforms (NMNAT1 or NMNAT3), NMNAT enzyme activity (i.e. NAD/NADH levels) or microtubule associated protein-2 (MAP2) or neurofilament protein levels. We therefore compared the impact of NMNAT2 knockdown on the development and maintenance of chemotherapy-induced peripheral neuropathy induced by vincristine and paclitaxel treatment using NMNAT2+/- and WT mice. NMNAT2+/- did not differ from WT mice in either the development or maintenance of either mechanical or cold allodynia induced by either vincristine or paclitaxel treatment. Intradermal injection of capsaicin, the pungent ingredient in hot chili peppers, produced equivalent hypersensitivity in NMNAT2+/- and WT mice receiving vehicle in lieu of paclitaxel. Capsaicin-evoked hypersensitivity was enhanced by prior paclitaxel treatment but did not differ in either NMNAT2+/- or WT mice. Thus, capsaicin failed to unmask differences in nociceptive behaviors in either paclitaxel-treated or paclitaxel-untreated NMNAT2+/- and WT mice. Moreover, no differences in motor behavior were detected between genotypes in the rotarod test. Our studies do not preclude the possibility that complete knockout of NMNAT2 in a conditional knockout animal could unmask a role for NMNAT2 in protection against detrimental effects of chemotherapeutic treatment.
The Impact of Delflex on Mesothelial Cell Viability and Peritoneal Transport
ClinicalTrials.gov study NCT01292863. IPD Sharing: Not stated. Countries: 0. Publications: 14.
Factors That Influence Adipose Derived Regenerative Cells' Yield and Viability
ClinicalTrials.gov study NCT02651233. IPD Sharing: NO. Countries: 0. Publications: 3.
Data from: Impact of genetic reduction of NMNAT2 on chemotherapy-induced losses in cell viability in vitro and peripheral neuropathy in vivo
Open the record for dataset details and reuse information.
Non-coding loci without epigenomic signals can be essential for maintaining global chromatin organization and cell viability [bulk RNA-seq]
GEO Series GSE176501. Homo sapiens. 3 samples. Type: Expression profiling by high throughput sequencing.
Non-coding loci without epigenomic signals can be essential for maintaining global chromatin organization and cell viability [single-cell RNA-seq]
GEO Series GSE176502. Homo sapiens. 1 samples. Type: Expression profiling by high throughput sequencing.
A BDNF-TrkB autocrine loop enhances senescent cell viability [scRNA-seq]
GEO Series GSE202950. Homo sapiens. 1 samples. Type: Expression profiling by high throughput sequencing.
High-throughput RNAi cell viability screen to identify selective targets for EWS-FLI1 positive Ewing sarcoma
GEO Series GSE73092. Homo sapiens. 9 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.