Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
10
datasets available to search
ShareScore release 0.9.0
Dataset results
10 results for “cellular uptake”
Cellular uptake of silica and gold nanoparticles induces early activation of nuclear receptor NR4A1
<p>The approval of new nanomedicines requires a deeper understanding of the interaction between cells and nanoparticles (NPs). Silica (SiO<sub>2</sub>) and gold (Au) NPs have shown great potential in biomedical applications, such as the delivery of therapeutic agents, diagnostics, and biosensors. NP-cell interaction and internalization can trigger several cellular responses, including gene expression regulation. The identification of differentially expressed genes in response to NP uptake contributes to a better understanding of the cellular processes involved, including potential side effects. We investigated gene regulation in human macrophages and lung epithelial cells after acute exposure to spherical 60 nm SiO<sub>2</sub> NPs. SiO<sub>2</sub> NPs uptake did not considerably affect gene expression in epithelial cells, whereas five genes were up-regulated in macrophages. These genes are principally related to inflammation, chemotaxis, and cell adhesion. NR4A1, an important modulator of inflammation in macrophages, was found to be up-regulated. The expression of this gene was increased upon 1 h of macrophage exposure to spherical 50 nm AuNPs and 200 nm spherical SiO<sub>2</sub> NPs. NR4A1 can thus be an important immediate regulator of inflammation provoked by NP uptake in macrophages.</p>
Core-shell structured chitosan-polyethylenimine nanoparticles for gene delivery: Improved stability, cellular uptake, and transfection efficiency
<p>Gene therapy has emerged as a promising treatment option for various acquired and inherited diseases. The delivery of nucleic acids relies on so-called vectors that condense and encapsulate their cargo, generating stable nano-sized particles. Especially non-viral gene delivery systems are of increasing interest. However, accomplishing therapeutic levels of transgene expression and limited tolerability of these systems remain a challenge. Therefore, we investigate in the present study the improvement of nucleic acid delivery using depolymerized chitosan – polyethylenimine DNA core complexes (dCS-PEI/DNA). These core complexes are further entrapped into a variety of dCS-based shells, functionalized with poly(ethylene glycol) (PEG) spacers conjugated to ionic moieties (amino or carboxylate groups) and cell penetrating peptides. This modular approach allowed to evaluate the effect of the shell functional components on the physico-chemical particle characteristics and biological effects <em>in vitro</em>. The optimized ternary complex combines a core-dCS-LPEI/DNA complex with a shell consisting of dCS-PEG-COOH, which resulted in improved encapsulation of nucleic acid, accelerated cellular uptake, enhanced transfection efficiency, and superior transfection potency in human hepatoma HuH-7 cells and mouse primary hepatocytes. Effects on transgene expression are confirmed <em>in vivo</em> in wild-type mice following retrograde intrabiliary infusion. After administration to mice of only 100 ng complexed nanovector DNA, ternary complexes induce a high reporter gene signal for three days. We conclude that ternary core-shell structured particles comprising functionalized chitosan are a promising gene delivery technology for both <em>in vitro</em> as well as <em>in vivo </em>applications. The modular design will facilitate the development of chemically modified derivatives.</p>
Dataset related to article "Tumor Treating Fields enhance chemotherapy efficacy by increasing cellular drug uptake and retention in mesothelioma cells"
<p>This record contains data related to article “Tumor Treating Fields enhance chemotherapy efficacy by increasing cellular drug uptake and retention in mesothelioma cells”</p> <p><span>Tumor Treating Fields (TTFields) applied with standard chemotherapy have been approved for the first-line treatment of unresectable pleural mesothelioma (PM), an aggressive malignancy with limited effective therapy options.</span></p> <p><span>In this study, we demonstrated that the simultaneous exposure to TTFields and doxorubicin or vinorelbine enhanced treatment efficacy in patient-derived PM cells by increasing intracellular drug concentrations. This was achieved by modulating several genes that encode transport proteins, such as the downregulation of P-glycoprotein.</span></p> <p><span>Using specific, sensitive and quantitative analytical techniques, we observed a more than 70% increase in intracellular concentrations of doxorubicin and vinorelbine in samples treated with TTFields, and a greater than 50% increase in drug uptake in cells exposed to TTFields and pemetrexed. This result indicates that the increased drug concentration observed in TTFields treated cells is significant not only for drugs that are P-gp substrates but also suggests that TTFields could potentially affect other efflux pumps. However, the co-exposure to the drug and TTFields was critical to increasing intracellular drug levels, highlighting the necessity of concurrent use with drugs to enhance the antiproliferative effects of treatment.</span></p> <p><span>The <em>in vitro</em> findings were further corroborated by <em>in vivo</em> pharmacokinetic experiments in mice subcutaneously injected with epithelioid PM tumors. Indeed, a 30% increase in intratumor concentrations was observed when vinorelbine was administered with TTFields.</span></p> <p><span>Our findings suggest that TTFields could be a well-tolerated approach for enhancing intratumoral drug levels and potentially achieving a more significant therapeutic impact on PM treatment.</span></p>
Cellular uptake of silica particles influences EGFR signaling pathway and is affected in response to EGF
<p><strong>Background:</strong> The human epidermal growth factor receptor (EGFR) is a receptor tyrosine kinase that is involved in several key cellular processes such as cell proliferation and differentiation, and it has been linked to the development and progression of various cancers (e.g. breast and lung). Researchers have attempted to improve current cancer-targeted therapies by conjugating molecules on the surface of (nano)particles to efficiently target and inhibit EGFR. However, very few in vitro studies have investigated the effect of particles per se on EGFR signaling and dynamics. Furthermore, the impact of concomitant exposure of particles and EGFR ligands, such as epidermal growth factor (EGF) on cellular uptake efficiency has received little attention. </p> <p><strong>Purpose: </strong>The purpose of this research was to determine the effects of silica (SiO2) particles on EGFR expression and intracellular signaling pathways in A549 lung epithelial cells, in the presence or absence of epidermal growth factor (EGF).</p> <p><strong>Results: </strong>We showed that A549 cells are able to internalize SiO<sub>2</sub> particles with core diameters of 130 nm and 1 µm without affecting cell proliferation or migration. However, both SiO<sub>2</sub> particles interfere with the EGFR signaling pathway by raising the endogenous levels of extracellular signal-regulated kinase (ERK) 1/2. Furthermore, both in the presence and absence of SiO<sub>2</sub> particles, the addition of EGF increased cell migration. EGF also stimulated cellular uptake of 130 nm SiO<sub>2</sub> particles but not 1 µm particles. The increased uptake is primarily associated with EGF-stimulated macropinocytosis.</p> <p><strong>Conclusion: </strong>This study shows that SiO<sub>2</sub> particle uptake interferes with cellular signaling pathways and can be boosted by concurrent exposure to the bioactive molecule EGF. SiO<sub>2</sub> particles, both alone and in combination with the ligand EGF, interfere with EGFR signaling pathway in a size-dependent manner.</p>
Selenium-Centered Cascade Exchangers and Conformational Control Unlock Unique Patterns of Thiol-Mediated Cellular Uptake: Original Data
<p>Original data and procedures for the synthesis of compounds <strong>8</strong>, <strong>13</strong>, <strong>28</strong>, and <strong>29</strong>, and cell experiments </p>
Automated high-content imaging for cellular uptake, from the Schmuck cation to the latest cyclic oligochalcogenides
<p>Original data</p>
Loss of insulin signaling in microglia impairs cellular uptake of Aβ and neuroinflammatory response exacerbating AD-like neuropathology
GEO Series GSE296386. Mus. 6 samples. Type: Other.
Tumor-associated macrophages derived from circulating inflammatory monocytes degrade collagen through cellular uptake
GEO Series GSE107053. Mus musculus. 8 samples. Type: Expression profiling by high throughput sequencing.
Tracking tau and cellular responses in human iPSC-microglia from uptake to seedable secretion in extracellular vesicles
GEO Series GSE291195. Homo sapiens. 24 samples. Type: Expression profiling by high throughput sequencing.
Biomolecular tracking by FIRESCAPE reveals distinct modes of clearance, damage induction and cellular uptake for extracellular histone H3
GEO Series GSE276733. Rattus norvegicus. 11 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.