Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
35
datasets available to search
ShareScore release 0.9.0
Dataset results
35 results for “cereal crop”
A global dataset gathering 37 field experiments involving cereal-legume intercrops and their corresponding sole crops.
<p>The overall description of the dataset is reported in the <strong>data_report.pdf</strong> file. The methodology for data curation and tidying is published in Peer Community Journal (<a href="https://doi.org/10.24072/pcjournal.389">Mahmoud2024</a>).</p> <p>This dataset gathers the results of 37 field experiments, which involved cereal-legume intercrops and their corresponding sole crops. The field experiments were carried in 5 European countries (France, Denmark, Italy, Germany and England) from 2001 to 2017. The dataset includes:</p> <ul> <li>5 legume species , <em>i.e.</em> chickpea (<em>Cicer arietinum</em> L.), faba bean (<em>Vicia faba</em> L.), lentil (<em>Lens culinaris</em> Med.), lupin (<em>Lupinus albus</em> L.) and pea (<em>Pisum sativum</em> L.),</li> <li>3 cereal species, <em>i.e.</em> barley (<em>Hordeum vulgare</em> L.), durum wheat (<em>Triticum turgidum</em> L.) and soft wheat (<em>Triticum aestivum</em> L.), </li> <li>8 resulting intercrops, <em>i.e.</em> i) barley associated with faba bean, lupin or pea, ii) durum wheat associated with chickpea, faba bean or pea, and iii) soft wheat associated with lentil or pea. </li> </ul> <p>In total, the dataset contains 299 sole crop and 308 intercrop experimental units, one given experimental unit being defined as the unique combination of {site, year, crop management}, with the crop management including species and cultivar choice as well as agricultural interventions (sowing conditions, inputs).</p> <p>The global dataset includes four tables, all sharing a common identifier (experiment_id):</p> <ul> <li>data_trials.csv: the global features describing the experimental sites,</li> <li>data_management.csv: the agricultural management actions carried out on each of the experimental sites,</li> <li>data_traits.csv: measured plant and crop characteristics,</li> <li>data_climate.csv: climate for the experimental sites, retrieved from NASA POWER API.</li> </ul> <p>Additionally, a metadata file is provided (<strong>metadata.xlsx</strong>), describing the table to which the variables belong (variable_type, i.e. trials, management, traits or climate), their name (variable_name), their significance (description) and their unit (unit). Finally, a table including the original references related to experimental files gathered (<strong>references.xlsx</strong>) is also provided.</p> <p>Data providers and field experiments: Laurent Bedoussac, Eric Justes, Etienne-Pascal Jour- net, Christophe Naudin, Henrik Hauggaard-Nielsen, Erik Steen Jensen, Elise Pelzer, Guénaëlle Corre-Hellou, Bochra Kammoun, Loic Viguier, Romain Barillot, Antoine Couëdel, Philippe Hinsinger</p> <p>Database and management: Noémie Gaudio, Rémi Mahmoud, Pierre Casadebaig</p>
Density independent prey choice, taxonomy, life history and web characteristics determine the diet and biocontrol potential of spiders (Linyphiidae and Lycosidae) in cereal crops - Dataset
<p>Materials and Methods</p> <p>Fieldwork</p> <p>Money spiders (Araneae: Linyphiidae) and wolf spiders (Araneae: Lycosidae) were the two most common families present in these field surveys, so were prioritised for collection. Spiders were visually located along transects in two adjacent barley fields at Burdons Farm, Wenvoe in South Wales (51°26'24.8"N, 3°16'17.9"W) and collected from occupied webs and the ground, between April and September 2018. Surveys and sampling were conducted five days per week across this period. Each transect was adjacent to a randomly selected tramline and they were distributed across the entire field. The areas searched were 4 m<sup>2</sup> quadrats at least 10 m apart and all observed linyphiids and lycosids were collected in approximately 15-minute searches. The spiders included in this study were taken from 64 locations across 24 days (Supplementary Table 3) along the aforementioned transects. Spiders were individually placed into 1.5 ml microcentrifuge tubes containing 100 % ethanol using an aspirator, regularly changing meshing, at least every five spiders, to limit potential cross-contamination between spiders (spiders were also subsequently washed during transferral to fresh ethanol at the identification and, separately, dissection stages). Linyphiids occupying webs were prioritised for collection, but ground-active linyphiid spiders were also collected. For each spider taken from a web, the height of the web from the ground and its approximate dimensions were recorded, the latter calculated as approximate web area. Spiders were taken to Cardiff University, transferred to fresh ethanol, adults identified to species-level and juveniles to genus, and stored at -80 °C in 100 % ethanol until subsequent DNA extraction. To obtain data on local prey density, 4 m<sup>2</sup> of ground and crop stems were suction sampled using a ‘G-vac’ for 30 seconds at each quadrat from which spiders were collected, with the collected material emptied into a bag, any organisms immediately killed with ethyl-acetate and material frozen for storage before sorting into 70 % ethanol in the lab.</p> <p>All invertebrates were identified to family level due to the restriction of many of the metabarcoding-derived dietary data to this level, and the difficulty associated with finer taxonomic resolution of many taxa. Exceptions included springtails of the superfamily Sminthuroidea (Sminthuridae and Bourletiellidae, which were often indistinguishable following suction sampling and preservation due to the fine features necessary to distinguish them) which were left at super-family, mites (many of which were immature or in poor condition) which were identified to order level and wasps of the superfamily Ichneumonoidea (which were identified no further due to obscurity of wing venation due to damage).</p> <p> </p> <p>Extraction and high-throughput sequencing of spider gut DNA</p> <p>Given their prevalence in field collections, dietary analysis was carried out for the linyphiid genera <em>Erigone</em>, <em>Tenuiphantes</em>, <em>Bathyphantes</em> and <em>Microlinyphia </em>(Araneae: Linyphiidae), and the Lycosidae genus <em>Pardosa</em>. Spiders were transferred to and washed in fresh 100 % ethanol to reduce external contaminants prior to identification via morphological key <sup>1</sup>. Abdomens were removed from spiders and again washed in and transferred to fresh 100 % ethanol. DNA was extracted from the abdomens via Qiagen TissueLyser II and DNeasy Blood & Tissue Kit (Qiagen) as per the manufacturer protocol, but with an extended lysis time of 12 hours to account for the complex and branched gut system in spider abdomens <sup>2</sup>. At least one extraction negative (blank tubes treated identically to samples) was included per 12 spiders (each extraction typically contained 24 spiders, thus two extraction negatives), which was included in subsequent PCR and high-throughput sequencing to detect instances of lab/reagent contamination.</p> <p>For amplification of DNA, two primer pairs were used. BerenF-LuthienR <sup>3</sup> amplified a broad range of invertebrates including spiders, and TelperionF-LaureR, amplified a range of invertebrates but fewer spiders (modified from TelperionF-LaurelinR <sup>3</sup> via one base-pair change from Laurelin; 5’-ggrtawacwgttcawccagt-3’). Primers were labelled with unique 10 bp molecular identifier tags (MID-tags) so that each individual had a unique pairing of forward and reverse tags for identification of each spider post-sequencing. PCR reactions of 25 µl contained 12.5 µl Qiagen PCR Multiplex kit, 0.2 µmol (2.5 µl of 2 µM) of each primer and 5 µl template DNA. Reactions were carried out in the same thermocycler, optimised via temperature gradient, with an initial 15 minutes at 95 °C, 35 cycles of 95 °C for 30 seconds, the primer-specific annealing temperature for 90 seconds and 72 °C for 90 seconds, respectively, followed by a final extension at 72 °C for 10 minutes. BerenF-LuthienR and TelperionF-LaureR used annealing temperatures of 52 °C and 42 °C, respectively.</p> <p>Within each PCR 96-well plate, 12 negative controls (extraction and PCR), 2 blank controls and 2 positive controls were included (i.e. 80 samples per plate), based on Taberlet <em>et al. </em>(2018). Positive controls were mixtures of invertebrate DNA comprised of non-native Asiatic species in four different proportions (Supplementary Table 1) and blanks were empty wells within each plate to identify tag-jumping into unused MID-tag combinations. PCR negative controls were DNase-free water treated identically to DNA samples. A negative control was present for each MID-tag to identify any contamination of primers. All PCR products were visualised in a 2 % agarose gel with SYBRSafe (Thermo Fisher Scientific, Paisley, UK) and placed in categories based on their relative brightness. The concentration of these brightness categories was quantified via Qubit dsDNA High-sensitivity Assay Kits (Thermo Fisher Scientific, Waltham, MA, USA) with at least three representatives of each category per plate. The PCR products were then proportionally pooled according to these concentrations. Each pool was cleaned via SPRIselect beads (Beckman Coulter, Brea, USA), with a left-side size selection using a 1:1 ratio (retaining ~300-1000 bp fragments). The concentration of the pooled DNA was then determined via Qubit dsDNA High-sensitivity Assay Kits and pooled together into one library per primer pair. Library preparation for Illumina sequencing was carried out on the cleaned libraries via NEXTflex Rapid DNA-Seq Kit (Bioo Scientific, Austin, USA) and samples were sequenced on an Illumina MiSeq via a V3 chip with 300-bp paired-end reads (expected capacity ≤25,000,000 reads). Bioinformatic analysis followed (Drake et al., 2021; Supplementary Information 1).</p> <p> </p> <p>Statistical analysis</p> <p>All analyses were conducted in R v4.0.0 <sup>6</sup>. Initial multivariate analyses used binary data (i.e., presence/absence) given the various problems inherent to quantifying metabarcoding data <sup>7,8</sup>. Prey species that occurred only once across all of the dietary samples were removed before further analyses to prevent outliers skewing the results, which is particularly problematic for non-metric multidimensional scaling. Spider diets were compared between variables using multivariate generalized linear models (MGLMs) via ‘manyglm’ in the ‘mvabund’ package <sup>9</sup> with a binomial error family and Monte Carlo resampling. Model independent variables included spider genus, spider life stage (juvenile or adult, the latter defined by fully developed genitalia), spider sex and all two-way interactions between these variables. Pairwise two-way interactions were also included between the aforementioned variables and Julian day to account for how seasonality may affect these relationships.</p> <p>Coarse dietary differences were visualised by non-metric multidimensional scaling (NMDS) via metaMDS in the ‘vegan’ package <sup>10</sup> with Jaccard distance in two dimensions and 999 tries. For NMDS, outliers (usually samples containing rare taxa) were identified by plotting and subsequently removed to facilitate separation of samples and achieve minimum stress. For visualisation of the effect of categorical variables against the dietary NMDS, spider plots were created using ‘ordispider’ with ‘ggplot’ and the ‘RColorBrewer’ ‘Accent’ colour palette <sup>11</sup>. Spider diet was compared against web characteristics for spiders for which both data were available using the MGLM process outlined above, but with starting models containing web height, web area, an interaction between the two, and pairwise interactions between genus, life stage and sex with the two web variables. This model used the same binomial error family as above, but with a ‘cloglog’ link function. For visualisation of the effect of continuous variables against the NMDS, surf plots were created with scaled coloured contours using the function “ordisurf” of the “ggplot” package in R.</p> <p>All prey taxa were classified as agricultural pests, natural enemies or excluded from subsequent analyses of intraguild predation and biocontrol (Supplementary Table 2). Intraguild predation and biocontrol variables were created by counting the number of natural enemy taxa, and, separately, of agriculturally relevant “pest” taxa (taxa containing species that commonly adversely affect agricultural productivity; Supplementary Table 2) in each spider’s diet. These resultant count data (effectively the diversity of pests and natural enemies predated by each individual spider) were separately analysed against spider genus, life stage and sex via GLM. “Site” (denoting the 4 m<sup>2</sup> area from which spiders were collected within fields) was initially included as a random effect in generalized linear mixed-models, but no significant effect was observed when comparing this model against a standard GLM via a likelihood ratio test of nested models using the ‘lrtest’ command in the ‘lmtest’ package <sup>12</sup>. Standard GLMs were thus used to avoid issues relating to singularity in the mixed models. The assumptions for the resultant Poisson error family GLMs were tested using the “testResiduals” function of the ‘DHARMa’ package <sup>13</sup>. Intraguild predation and biocontrol differences between significant terms were visualised using violin plots with the quartiles, median and 95 % upper limit annotated using the ‘geom_violin’ function in ‘ggplot2’.</p> <p><em>In situ</em> spider prey choice was analysed using network-based null models in the ‘econullnetr’ package <sup>14</sup> with the ‘generate_null_net’ command, visually represented with the ‘plot_preferences’ command. Binary dietary data were used alongside suction sample count data to represent prey availability. These suction sample data, as described above, were collected at the same sites as the spiders three days after spider collection. Prior to the taxonomic prey choice analysis, an hemipteran identified no further than order level through dietary analysis was removed due to the inability to pair it to any present prey taxa with certainty. Standardised effect sizes (SES) were extracted for all comparisons for each individual spider and compared between genera, life stages and sexes using permutational multivariate analysis of variance (PerMANOVA) using the ‘adonis’ function of the ’vegan’ package with 9999 permutations and a Euclidean distance matrix to determine overall differences in prey choice.</p> <p> </p> <p>References</p> <p>1. Roberts, M. J. <em>The Spiders of Great Britain and Ireland (Compact Edition)</em>. (Harley Books, 1993).</p> <p>2. Krehenwinkel, H., Kennedy, S., Pekár, S. & Gillespie, R. G. A cost-efficient and simple protocol to enrich prey DNA from extractions of predatory arthropods for large-scale gut content analysis by Illumina sequencing. <em>Methods Ecol. Evol.</em> <strong>8</strong>, 126–134 (2017).</p> <p>3. Cuff, J. P. <em>et al.</em> Money spider dietary choice in pre- and post-harvest cereal crops using metabarcoding. <em>Ecol. Entomol.</em> <strong>46</strong>, 249–261 (2021).</p> <p>4. Taberlet, P., Bonin, A., Zinger, L. & Coissac, E. <em>Environmental DNA</em>. (Oxford University Press, 2018).</p> <p>5. Drake, L. E. <em>et al.</em> An assessment of minimum sequence copy thresholds for identifying and reducing the prevalence of artefacts in dietary metabarcoding data. <em>Methods Ecol. Evol.</em> <strong>in press</strong>, (2021).</p> <p>6. R Core Team. R: A language and environment for statistical computing. (2020).</p> <p>7. Deagle, B. E., Thomas, A. C., Shaffer, A. K. & Trites, A. W. Quantifying sequence proportions in a DNA-based diet study using Ion Torrent amplicon sequencing: which counts count? <em>Mol. Ecol. Resour.</em> <strong>13</strong>, 620–633 (2013).</p> <p>8. Deagle, B. E. <em>et al.</em> Counting with DNA in metabarcoding studies: How should we convert sequence reads to dietary data? <em>Mol. Ecol.</em> <strong>28</strong>, 391–406 (2019).</p> <p>9. Wang, Y., Naumann, U., Wright, S. T. & Warton, D. I. mvabund – an R package for model-based analysis of multivariate abundance data. <em>Methods Ecol. Evol.</em> <strong>3</strong>, 471–474 (2012).</p> <p>10. Oksanen, J. <em>et al.</em> vegan: Community Ecology Package. (2016).</p> <p>11. Neuwirth, E. RColorBrewer: ColorBrewer palettes. (2014).</p> <p>12. Zeileis, A. & Hothorn, T. Diagnostic checking in regression relationships. <em>R News</em> <strong>2</strong>, 7–10 (2002).</p> <p>13. Hartig, F. DHARMa: residual diagnostics for hierarchical (multi-level/mixed) regression models. (2020).</p> <p>14. Vaughan, I. P. <em>et al.</em> econullnetr: an r package using null models to analyse the structure of ecological networks and identify resource selection. <em>Methods Ecol. Evol.</em> <strong>9</strong>, 728–733 (2018).</p>
Dataset for the article "Barriers and Opportunities for Sustainable Farming Practices and Crop Diversification Strategies in Mediterranean Cereal-Based Systems"
<p>Datasets from the surveys applied for the article "Barriers and Opportunities for Sustainable Farming Practices and Crop Diversification Strategies in Mediterranean Cereal-Based Systems" <a href="https://doi.org/10.3389/fenvs.2022.861225">https://doi.org/10.3389/fenvs.2022.861225</a></p>
Early-season biomass and weather enable robust cereal rye cover crop biomass predictions
<p>Farmers need accurate estimates of winter cover crop biomass to make informed decisions on termination timing or to estimate potential release of nitrogen from cover crop residues to subsequent cash crops. Utilizing data from an extensive experiment across 11 states from 2016 to 2020, this study explores the most reliable predictors for determining cereal rye cover crop biomass at the time of termination. Our findings demonstrate a strong relationship between early-season and late-season cover crop biomass. Employing a random forest model, we predicted late-season cereal rye biomass with a margin of error of approximately 1,000 kg ha<sup>-1</sup> based on early-season biomass, growing degree days, cereal rye planting and termination dates, photosynthetically active radiation, precipitation, and site coordinates as predictors. Our results suggest that similar modeling approaches could be combined with remotely sensed early-season biomass estimations to improve the accuracy of predicting winter cover crop biomass at termination for decision support tools.</p>
U.S. cereal rye winter cover crop growth database
<p>Winter cover crop performance metrics (i.e., vegetative biomass quantity and quality) affect ecosystem services provisions but vary widely due to differences in agronomic practices, soil properties, and climate. Cereal rye (Secale cereale) is the most common winter cover crop in the United States due to its winter hardiness, low seed cost, and high biomass production. We compiled data on cereal rye winter cover crop performance metrics, agronomic practices, and soil properties across the eastern half of the United States. The dataset includes a total of 5,695 cereal rye biomass observations across 208 site-years between 2001–2022 and encompasses a wide range of agronomic, soil, and climate conditions. Cereal rye biomass values had a mean of 3,428 kg ha−1, a median of 2,458 kg ha−1, and a standard deviation of 3,163 kg ha−1. The data can be used for empirical analyses, to calibrate, validate, and evaluate process-based models, and to develop decision support tools for management and policy decisions.</p>
Data for : Effects of electrokinetic and ultrasonication pre-treatment and two-step anaerobic digestion of biowastes on the nitrogen fertiliser value by injection or surface banding to cereal crops
<p>Data file for article: Effects of electrokinetic and ultrasonication pre-treatment and two-step anaerobic digestion of biowastes on the nitrogen fertiliser value by injection or surface banding to cereal crops (https://doi.org/10.1016/j.jenvman.2022.116699).</p>
Supplementary material for: "Seeds adapted to mixed cropping increase yield and drought resistance of cereal-legume mixtures"
<p>Supplementary material for the research article: "Seeds adapted to mixed cropping increase yield and drought resistance of cereal-legume mixtures".</p> <ul> <li>Raw data</li> <li>R analysis code</li> <li>Statistical analysis info: ANOVA and Tukey comparisons tables</li> </ul> <p> </p> <p>Abstract:</p> <p>Cropland diversification through mixed cropping has the potential of achieving a more sustainable agriculture while securing food production. This is of special relevance with climate change and the expected drier growing conditions in the future. Seed adaptation to this cropping method is hypothesised to be a fundamental factor to maximise these benefits, as well as the particular species combined. In this study we compared the performance of four cereal-legume mixed crops (wheat and oat mixed with lupin and lentil in pairs) with their respective monocrops. Each crop was sown using seeds adapted to monoculture and mixed cropping, respectively. Moreover, they were grown under early-season and late-season drought treatments and under control conditions. We measured above-ground vegetative biomass, seed yield and harvest index to evaluate crop production, drought resistance and the effect of seed adaptation on each mixed and monocrop. Our results show that mixed cropping either had a beneficial or neutral effect on crop yield, depending on the species combination and drought conditions, but harvest index was generally higher in monocrops. We also confirmed that seed adaptation to a particular type of cropping is clearly a determining factor in its performance. In accordance with the insurance hypothesis, mixed cropping has the effect of protecting crop yields in the case of a sudden bad performance of one of the species, for example, caused by adverse environmental conditions. It is necessary to focus on effective species combinations which have the best responses to mixed cropping. We show for the first time that wheat-lentil mixtures performed poorly, while wheat-lupin showed the most promising results improving yield and drought resistance. Oat mixed crops did not show differences with the respective monocrops, so they can be a viable cropping option as well and benefit from advantages of crop diversity not measured in this study.</p>
Dataset for "Species diversity and molecular characterization of Alternaria section Alternaria isolates collected mainly from cereal crops in Canada" by Jeremy R. Dettman, Quinn Eggertson, and Natalie E. Kim
<p>Dataset consists of three files, each containing aligned nucleotide sequences from 559 Alternaria strains. The three sequenced loci are ASA-10, ASA-19, and rpb2. Strain names are stated in the header of each sequence.</p>
Selective sweeps identification in distinct groups of cultivated rye (Secale cereale L.) germplasm provides potential candidate genes for crop improvement
<p><strong>Background</strong></p> <p>During domestication and subsequent improvement, plants were subjected to intensive positive selection for desirable traits. Identification of selection targets is important with respect to the future targeted broadening of diversity in breeding programmes. Rye (<em>Secale</em> <em>cereale</em> L.) is a cereal that is closely related to wheat, and it is an important crop in Central, Eastern and Northern Europe. The aim of the study was (i) to identify diverse groups of rye accessions based on high-density, genome-wide analysis of genetic diversity within a set of 478 rye accessions, covering a full spectrum of diversity within the genus, from wild accessions to inbred lines used in hybrid breeding, and (ii) to identify selective sweeps in the established groups of cultivated rye germplasm and putative candidate genes targeted by selection. <strong> </strong></p> <p><strong>Results</strong></p> <p>Population structure and genetic diversity analyses based on high-quality SNP (DArTseq) markers revealed the presence of three complexes in the <em>Secale</em> genus: <em>S. sylvestre, S. strictum </em>and<em> S. cereale/vavilovii</em>, a relatively narrow diversity of <em>S. sylvestre</em>, very high diversity of <em>S. strictum</em>, and signatures of strong positive selection in <em>S. vavilovii</em>. Within cultivated ryes, we detected the presence of genetic clusters and the influence of improvement status on the clustering. Rye landraces represent a reservoir of variation for breeding, and especially a distinct group of landraces from Turkey should be of special interest as a source of untapped variation. Selective sweep detection in cultivated accessions identified 133 outlier positions within 13 sweep regions and 170 putative candidate genes related, among others, to response to various environmental stimuli (such as pathogens, drought, cold), plant fertility and reproduction (pollen sperm cell differentiation, pollen maturation, pollen tube growth), and plant growth and biomass production.</p> <p><strong>Conclusions</strong></p> <p>Our study provides valuable information for efficient management of rye germplasm collections, which can help to ensure proper safeguarding of their genetic potential and provides numerous novel candidate genes targeted by selection in cultivated rye for further functional characterisation and allelic diversity studies.</p>
Selective sweeps identification in distinct groups of cultivated rye (Secale cereale L.) germplasm provides potential candidate genes for crop improvement
Open the record for dataset details and reuse information.
Early-season biomass and weather enable robust cereal rye cover crop biomass predictions
Open the record for dataset details and reuse information.
U.S. cereal rye winter cover crop growth database
Open the record for dataset details and reuse information.
Sustainable landscape, soil and crop management practices enhance biodiversity and yield in conventional cereal systems
<p>1. Input-driven, modern agriculture is commonly associated with large-scale threats to biodiversity, the disruption of ecosystem services and long-term risks to food security and human health. A switch to more sustainable yet highly productive farming practices seems unavoidable. However, an integrative evaluation of targeted management schemes at field and landscape scales is currently lacking. Furthermore, the often-disproportionate influence of soil conditions and agrochemicals on yields may mask the benefits of biodiversity-driven ecosystem services.</p> <p>2. Here, we used a real-world ecosystem approach to identify sustainable management practices for enhanced functional biodiversity and yield on 28 temperate wheat fields. Using path analysis, we assessed direct and indirect links between soil, crop and landscape management with natural enemies and pests, as well as follow-on effects on yield quantity and quality. A paired-field design with a crossed insecticide-fertilizer experiment allowed us to control for the relative influence of soil characteristics and agrochemical inputs.</p> <p>3. We demonstrate that biodiversity-enhancing management options such as reduced tillage, crop rotation diversity and small field size can enhance natural enemies without relying on agrochemical inputs. Similarly, we show that in this system controlling pests and weeds by agrochemical means is less relevant than expected for final crop productivity.</p> <p>4. Synthesis and applications: Our study highlights soil, crop and landscape management practices that can enhance beneficial biodiversity while reducing agrochemical usage and negative environmental impacts of conventional agriculture. The diversification of cropping systems and conservation tillage are practical measures most farmers can implement without productivity losses. Combining local measures with improved landscape management may also strengthen the sustainability and resilience of cropping systems in light of future global change.</p>
Dataset: Floral presence and flower identity alter cereal aphid endosymbiont communities on adjacent crops
<ol> <li>Floral plantings adjacent to crop fields can recruit populations of natural enemies by providing flower nectar and non-crop prey to increase natural pest regulation. Observed variation in success rates might be due to changes in the unseen community of endosymbionts hosted by many herbivorous insects, of which some can confer resistance to natural enemies, e.g. parasitoid wasps. Reduced insect control may occur if highly protective symbiont combinations increase in frequency via selection effects, and this is expected to be stronger in lower diversity systems.</li> <li>We used a large-scale field trial to analyse the bacterial endosymbiont communities hosted by cereal aphids (<em>Sitobion</em> <em>avenae</em>) collected along transects into strip plots of barley plants managed by either conventional or integrated (including floral field margins and reduced inputs) methods. In addition, we conducted an outdoor pot experiment to analyse endosymbionts in <em>S. avenae</em> aphids collected on barley plants that were either grown alone or alongside one of three flowering plants, across three time points.</li> <li>In the field, aphids hosted up to four symbionts. The abundance of aphids and parasitoid wasps was reduced towards the middle of all fields while aphid symbiont species richness and diversity decreased into the field in conventional, but not integrated, field-strips. The proportion of aphids hosting different symbiont combinations varied across cropping systems, with distances into the fields, and was correlated with parasitoid wasp abundances.</li> <li>In the pot experiment, aphids hosted up to six symbionts. Flower presence increased natural enemy abundance and diversity, and decreased aphid abundance. The proportion of aphids hosting different symbiont combinations varied across the flower treatment and time and were correlated with varying abundances of the different specialist parasitoid wasp species recruited by different flowers.</li> <li> <em>Synthesis and applications.</em> Floral plantings and flower identity had community-wide impacts on the combinations of bacterial endosymbionts hosted by herbivorous insects, which correlated with natural enemy diversity and abundance. We recommend that integrated management practices incorporate floral resources within field areas to support a more functionally diverse and resilient natural enemy community to mitigate selection for symbiont-mediated pest resistance throughout the cropping area.</li> </ol>
Data from: Soil microbes alter herbivore-induced volatile emissions in response to cereal cropping systems
Open the record for dataset details and reuse information.
Sustainable landscape, soil and crop management practices enhance biodiversity and yield in conventional cereal systems
Open the record for dataset details and reuse information.
Dataset: Floral presence and flower identity alter cereal aphid endosymbiont communities on adjacent crops
Open the record for dataset details and reuse information.
Money spider dietary choice in pre- and post-harvest cereal crops using metabarcoding
<ol> <li>Money spiders (Linyphiidae) are an important component of conservation biological control in cereal crops, but they rely on alternative prey when pests are not abundant, such as between cropping cycles. To optimally benefit from these generalist predators, prey choice dynamics must first be understood.</li> <li>Money spiders and their locally available prey were collected from cereal crops two weeks pre- and post-harvest. Spider gut DNA was amplified with two novel metabarcoding primer pairs designed for spider dietary analysis, and sequenced.</li> <li>The combined general and spider-exclusion primers successfully identified prey from 15 families in the guts of the 46 linyphiid spiders screened, whilst avoiding amplification of <i>Erigone </i>spp. The primers show promise for application to the diets of other spider families such as Agelenidae and Pholcidae.</li> <li>Distinct invertebrate communities were identified pre- and post-harvest, and changes in spider diet and, to a lesser extent, prey choice reflected this. Spiders were found to consume one another more than expected, indicating their propensity toward intraguild predation, but also consumed common pest families.</li> <li>Changes in spider prey choice may redress prey community changes to maintain a consistent dietary intake. Consistent provision of alternative prey via permanent refugia should be considered to sustain effective conservation biocontrol.</li> </ol>
FIGURE 6. Cereal crop seeds a in First comprehensive study on distribution frequency and incidence of seed-borne pathogens from cereal and legume crops in Sri Lanka
FIGURE 6. Cereal crop seeds a Arachis hypogea (Tissa) b Oryza sativa (Bg251) c Vigna radiata (MI6) and d Vigna sinensis (Dhawala).
FIGURE 4 in First comprehensive study on distribution frequency and incidence of seed-borne pathogens from cereal and legume crops in Sri Lanka
FIGURE 4. Colony morphology (upper surface and lower surface) and microscopic features (conidia and conidiophore) respectively of fungal pure cultures from Vigna radiata on PDA after 10 days at 28–30 0C; a–c Rhizopus oryzae.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.