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opencc-by-4.0Feb 2022View details →

High resolution 3D reconstruction of regenerating nerve within a chitosan conduit 7 days after injury and repair

Video S1: high resolution 3D reconstruction of 7 consecutive 50 µm thick sections labelled with Reca1 (red, endothelial cell marker) and S100β (green, Schwann cell marker).

opencc-by-4.0May 2022View details →

Additional data; Biotechnologically produced chitosans with nonrandom acetylation patterns differ from conventional chitosans in properties and activities

This dataset contains additional data for the research article Biotechnologically produced chitosans with nonrandom acetylation patterns differ from conventional chitosans in properties and activities by Sruthi Sreekumar, Jasper Wattjes, Anna Niehues, Tamara Mengoni, Ana C. Mendes, Edwin R. Morris, Francisco M. Goycoolea, and Bruno M. Moerschbacher. The directory Fig1ab_SuppFig1ab contains data, code, and results of enzymatic mass-spectrometric fingerprinting experiments. The directory cosms contains source code and instructions to create a conda environment in which the code to create Figures 1 A+B and Supplementary Figures 1 A+B can be executed. The directory Fig23456_SuppFig_2567 contains Excel Worksheets (.xlsx files) with data underlying Figures 2, 3, 4, 5 and 6, and Supplementary Figures 2, 5, 6 and 7. The directories Fig5b and SuppFig7d contain raw MS data (Bruker .d files) of oligomeric hydrolysis products produced by incubating different chitosans with different chitinolytic enzymes.

opencc-by-4.0Jun 2024View details →

Data for the paper: The Role of Glycerol in Manufacturing Freeze-Dried Chitosan and Cellulose Foams for Mechanically Stable Scaffolds in Skin Tissue Engineering

The Dataset contains all the data, described in the article "The Role of Glycerol in Manufacturing Freeze-Dried Chitosan  and Cellulose Foams for Mechanically Stable Scaffolds in Skin Tissue Engineering." Abstract Various strategies have extensively explored enhancing the physical and biological properties of chitosan and cellulose scaffolds for skin tissue engineering. This study presents a straightforward method involving the addition of glycerol into highly porous structures of two polysaccharide complexes: chitosan/carboxymethyl cellulose (Chit/CMC) and chitosan/oxidized cellulose (Chit/OC); during a one-step freeze-drying process. Adding glycerol, especially to Chit/CMC, significantly increased stability, prevented degradation, and improved mechanical strength by nearly 50%. Importantly, after 21 days of incubation in enzymatic medium Chit/CMC scaffold has almost completely decomposed, while foams reinforced with glycerol exhibited only 40% mass loss. It is possible due to differences in multivalent cations and polymer chain contraction, resulting in varied hydrogen bonding  and, consequently, distinct physicochemical outcomes. Additionally, the scaffolds with glycerol improved the cellular activities resulting in over 40% higher proliferation of fibroblast after 21 days of incubation. It was achieved by imparting water resistance to the highly absorbent material and aiding in achieving a balance between hydrophilic and hydrophobic properties. This study clearly indicates the possible elimination of additional crosslinkers and multiple fabrication steps that can reduce the cost of scaffold production for skin tissue engineering applications while tailoring mechanical strength and degradation. Figure 2. Morphology. SEM micrographs of the internal structure of the freeze-dried scaffolds. Results of porosity analysis. The methodology and data are described in the README file in the folder. Figure 3. Mechanical test results. Representative stress-strain curves from the tensile test of all freeze-dried scaffolds, where (A) – measurement performed in dry conditions, (B) – measurement performed in wet conditions. All are described in the README file in the folder. Figure 4. Swelling behavior of all scaffolds. B – Two representative vials with a visual demonstration of swelling, samples marked with circles: Chit/CMC sample submerged in the PBS (blue circle) and Chit/CMC/Glyc sample floating on the surface (green circle). The arrows lead to photos of scaffolds taken from vials directly after swelling. C – Gel fraction analysis in aqueous solution after 24  6 h. D – Time after which the water droplet is absorbed into the scaffold. E – Photographs of water droplet shape changes on Chit/CMC and Chit/CMC/Glyc scaffolds over time. All are described in the README file in the folder. Figure 5. Fourier Transform Infrared Spectroscopy (ATR-FTIR) analysis results. Details are in the README file in the folder. Figure 6. The FTIR spectra of eluates from degraded scaffolds collected on a microscopic glass slide. Details are in the README file in the folder. Figure 7.  The degradation studies of all scaffolds over 21 days of experiments in A – enzymatic medium. B – cell culture medium. Details are in the README file in the folder. Figure 9. Cell experiments and toxicity analysis. Cytotoxicity of eluates taken from degraded scaffolds. B – Direct fibroblast seeding on scaffolds during 14 days of culture period. C – Direct fibroblast seeding on scaffolds during 14 days of culture period without control to better see the effect of glycerol. Details are in the README file in the folder.

Figure 5 in Evaluation of chitosan and silver nanoparticles Against isolated pathogens from Mulberry Silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) under laboratory conditions

Figure 5. TEM micrograph of chitosan nanoparticles prepared by ionic gelation method. Table 3. Antimicrobial activity of silver nanoparticles (µl) with different concentrations against fungal and bacterial isolates.

Figure 1 in Evaluation of chitosan and silver nanoparticles Against isolated pathogens from Mulberry Silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) under laboratory conditions

Figure 1. Mean weight of healthy and infected fourth and fifth instar larvae of B. mori. Isolation and identification of bacterial isolates: Total of 7 bacterial were successfully isolated from the outer surface and the inner bоdy of silkworm larvae.

opencc-by-4.0Jul 2022View details →

Core-shell structured chitosan-polyethylenimine nanoparticles for gene delivery: Improved stability, cellular uptake, and transfection efficiency

Gene therapy has emerged as a promising treatment option for various acquired and inherited diseases. The delivery of nucleic acids relies on so-called vectors that condense and encapsulate their cargo, generating stable nano-sized particles. Especially non-viral gene delivery systems are of increasing interest. However, accomplishing therapeutic levels of transgene expression and limited tolerability of these systems remain a challenge. Therefore, we investigate in the present study the improvement of nucleic acid delivery using depolymerized chitosan – polyethylenimine DNA core complexes (dCS-PEI/DNA). These core complexes are further entrapped into a variety of dCS-based shells, functionalized with poly(ethylene glycol) (PEG) spacers conjugated to ionic moieties (amino or carboxylate groups) and cell penetrating peptides. This modular approach allowed to evaluate the effect of the shell functional components on the physico-chemical particle characteristics and biological effects in vitro. The optimized ternary complex combines a core-dCS-LPEI/DNA complex with a shell consisting of dCS-PEG-COOH, which resulted in improved encapsulation of nucleic acid, accelerated cellular uptake, enhanced transfection efficiency, and superior transfection potency in human hepatoma HuH-7 cells and mouse primary hepatocytes. Effects on transgene expression are confirmed in vivo in wild-type mice following retrograde intrabiliary infusion. After administration to mice of only 100 ng complexed nanovector DNA, ternary complexes induce a high reporter gene signal for three days. We conclude that ternary core-shell structured particles comprising functionalized chitosan are a promising gene delivery technology for both in vitro as well as in vivo applications. The modular design will facilitate the development of chemically modified derivatives.

Figure 4 in Evaluation of chitosan and silver nanoparticles Against isolated pathogens from Mulberry Silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) under laboratory conditions

Figure 4. Transmissiоn electron microscоpy micrоgraph of silver nanoparticles.

Figure 3 in Evaluation of chitosan and silver nanoparticles Against isolated pathogens from Mulberry Silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) under laboratory conditions

Figure 3. Final dispersion formed after reduction (A) silver and (B) chitosan.

opencc-by-4.0Aug 2023View details →

Dataset from "A one-pot chitosan pyrolysis in the presence of a ZnCl 2 /NaCl salts for carbons with electrocatalytic activity"

Dataset from manuscript entitled: "A one-pot chitosan pyrolysis in the presence of a ZnCl 2 /NaCl salts for carbons with electrocatalytic activity"

ClinicalTrials.gov36/100

Efficacy in Preventing Hypertrophic Scars: A Randomised Controlled Trial Comparing Chitosan Cream, Silicone Gel & Olive Oil

ClinicalTrials.gov study NCT07269093. IPD Sharing: NO. Countries: 1. Publications: 2.

opencc-by-4.0Aug 2020View details →

zenodo32/100

Selection of Water-Soluble Chitosan by Microwave-Assisted Degradation and pH Controlled Precipitation

Dataset for "Selection of Water-Soluble Chitosan by Microwave-Assisted Degradation and pH Controlled Precipitation" Polymers (2020) 12, 1274. DOI: 10.3390/polym12061274. Raw data for NMR spectra; raw GPC data; experimental descriptions; metadata file

dryad32/100

Resveratrol loaded chitosan-pectin core-shell nanoparticles as novel drug delivery vehicle for sustained release and improved antioxidant activities

Resveratrol, chemically known as 3, 5, 4'-trihydroxy-trans-stilbene, is a natural polyphenol with promising multi-targeted health benefits. The optimal therapeutic uses of resveratrol are limited due to its poor solubility, rapid metabolism and low bioavailability. To address the issues, we have encapsulated resveratrol inside the nanosized core made of chitosan and coated this core with pectin - shell in order to fabricate a drug delivery vehicle which can entrap resveratrol for a longer period of time. The core - shell nanoparticles fabricated in this way were characterized with the help of Fourier Transform Infrared Spectrometer (FTIR), Field Emission Scanning Electron Microscope (FESEM), Field Emission Transmission electron microscopy/Selected Area Electron Diffraction (FETEM/SAED), High-Resolution Transmission Electron Microscope (HRTEM)), Dynamic Light Scattering (DLS) and Zeta Potential measurements. In vitro drug release study showed the ability of the core-shell nanoparticles to provide sustained release of resveratrol for almost 30 hours. The release efficiency of the drug was found to be pH dependent and a sequential control over drug release can be obtained varying the shell thickness. The resveratrol encapsulated in a nanocarrier was found to have a better in vitro antioxidant activity than free resveratrol as determined by DPPH radical scavenging method. This work finally offers a novel nano-based drug delivery system.

opencc-zeroFeb 2022View details →

zenodo32/100

Data from: "Glycerol-blended chitosan membranes with directional micro-grooves and reduced stiffness improve Schwann cell wound healing"

<h3>ABSTRACT</h3> Regenerative medicine is continuously looking for new natural biocompatible and possibly biodegradable materials, but also mechanically compliant. Chitosan is emerging as a promising FDA-approved biopolymer for tissue engineering, however, its exploitation in regenerative devices is limited by its brittleness and can be further improved, for example, by blending it with other materials or by tuning its superficial microstructure. Here, we developed membranes made of chitosan and glycerol, by solvent casting and micropatterned them with directional geometries with different levels of axial symmetry. These membranes were characterized by light microscopy and atomic force microscopy (AFM), thermal, mechanical, and degradation assays, and also tested in vitro as scaffolds with Schwann cells. The glycerol-blended chitosan membranes are optimized in terms of mechanical properties, and present a physiological-grade Young's modulus (≈ 0.7 MPa). The directional topographies are effective in directing cell polarization and migration and in particular are highly performant substrates for collective cell migration. Here, we demonstrate that a combination of a soft compliant biomaterial and topographical micropatterning can improve the integration of these scaffolds with Schwann cells, which is a fundamental step in the peripheral nerve regeneration process.

opencc-by-4.0Aug 2024View details →

zenodo32/100

Evaluation of Chitosan based orally delivered insulin nanoparticle

Data sets Ethical approval Figures

opencc-by-4.0Aug 2023View details →

restrictedIPD-UNDECIDEDFeb 2026View details →

Safety of Chitosan as Wine Fining Agent in Shrimp Allergic Patients

ClinicalTrials.gov study NCT02151279. IPD Sharing: Not stated. Countries: 1. Publications: 1.

Treatment of Residual Pockets in Periodontal Patients Using an Oscillating Chitosan Device

ClinicalTrials.gov study NCT06127069. IPD Sharing: NO. Countries: 1. Publications: 1.

Clinical Trial Evaluating the Safety and Efficacy of the Use of Chitosan Gel in Patients With Chronic Wounds

ClinicalTrials.gov study NCT04178525. IPD Sharing: NO. Countries: 2. Publications: 1.

Antibacterial Effect and Clinical Performance of Chitosan Modified Glass Ionomer

ClinicalTrials.gov study NCT04365270. IPD Sharing: NO. Countries: 1. Publications: 5.