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Dataset of the paper entitled methods for high-throughput screening of novel agents against the maize pest, Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae)
<p>Title: Methods for high-throughput screening of novel agents against the maize pest, Diabrotica virgifera virgifera (Coleoptera: Chrysomelidae) </p> <p>Authors: Sri Ita Tarigan, Gyorgy Turoczi, Jozsef Kiss, Stefan Toepfer</p> <p>Abstract: <br>The western corn rootworm, <em>Diabrotica virgifera virgifera</em> (Coleoptera: Chrysomelidae), poses a significant threat to maize crops in North America and Europe, necessitating development of novel, effective, and less disruptive crop protection agents. With recent bans on key insecticides and concerns about overuse of remaining options, there is an urgent need for accessible and comparable screening methods. We propose comparative high-throughput screening methods against the eggs, larvae and adults of this pest, emphasizing the importance of suitable positive controls tailored to the specific bioassay types. We evaluated seven common insecticides (imidacloprid, clothianidin, acetamiprid, novaluron, cypermethrin, chlorpyrifos-methyl, spinosad) against eggs, larvae, and adults as potential positive controls for each of the proposed assay methods. Dipping assays with ready-to-hatch eggs revealed several ingredients to cause mortality; but imidacloprid might be most suitable as a positive control due to its robust dose-response in reducing egg hatching and causing mortality of hatching neonates. Larval bioassays using artificial diet overlay assays revealed mortality caused by all insecticides, with imidacloprid and acetamiprid exhibiting best dose-mortality response curves as well as sublethal effects. Adult bioassays using artificial diet-core overlay assays revealed mortality caused by all insecticides, with cypermethrin or acetamiprid exhibiting best dose-mortality response curves. The provided ED <sub>50</sub>, ED <sub>80</sub> values, and dose-response equations offer valuable insight for researchers in selecting appropriate positive controls for screening new crop protection agents or assessing resistance levels against different life stages of this pest.</p> <p>Data:</p> <p>The data file is related to the screening of commercial insecticides against eggs, first instar larvae (L1) and adults of the maize pest, <em>Diabrotica virgifera virgifera</em> using standard bioassays. We are proposing comparative high-throughput screening methods against the eggs, larvae and adults of this maize pest. This includes the crucial aspect of suitable positive controls tailored to the specific bioassay type. We evaluated seven common insecticides (imidacloprid, clothianidin, acetamiprid, novaluron, cypermethrin, chlorpyrifos-methyl, spinosad) against eggs, larvae, and adults as potential positive controls for each of the proposed assay method. To access effects and dose-responses of commonly used insecticides on eggs, we applied standard screening methods under controlled semi-sterile conditions.</p> <p>For egg bioassays, eggs were transferred to the 200 ml of treatments in the eppendorf tubes and then soaked for 1 hour. Then 20µl with 10 to 20 eggs were pipetted onto a filter paper in a petri dish (150 mm×25 mm). Then 100 µl of sterilized tap water was added for moisture. The pipette tip was replaced between treatments. The eggs been transferred were counted per filter paper and dish (15± 8). The eggs were then incubated in the dishes at 23-25<sup>0</sup>C for 7 days, when the experiment was terminated. Egg hatching, mortality of newly hatching larvae, and days until start of egg hatching were observed under stereo microscope and recorded. Data were collected at 1,3, 5 and 7 days after treatments.</p> <p>To assess the effect and dose-responses of commonly used insecticides on neonates of <em>D. v. virgifera</em>, we applied artificial diet-overlay bioassays under controlled semi-sterile conditions. Each insecticide was prepared in at least six concentrations. Each bioassay consisted of 3 to 6 polystyrene plates of 96 wells each (07-6096 of Biologix Ltd., USA, or Costar 3917 of Corning Inc., USA). Each well had a volume of 330 µl, with a diameter of 5 mm, a height of 10 mm, and a surface area of 0.34 cm². 190 µl of the diet were pipetted into each 330 µl well, filling each to approximately 2/3<sup>rd </sup>of its capacity. Plates containing the diet were left to dry in a laminar flow cabinet for 45 minutes and then stored overnight at temperatures ranging from 3 to 5°C. The following day, treatments were applied. This is, 17 µl of a treatment was applied to the 0.34 cm<sup>2</sup> diet surface reaching good coverage and therefore forcing the after-placed larvae to feed through (10 to 100 µl pipette Biohit TM Proline). Each treatment was applied to 8 wells per plate. Following application, the plates were allowed to dry for a duration of 1 to 1.5 hours and were subsequently cooled for 1 hour in a refrigerator set at temperatures between 23 to 25°C. Each well received one neonate larva, carefully placed on the diet surface using a fine artist brush. A vigorous and visibly healthy larva was selected, lifted from the end of the abdomen with the brush, maneuvered towards a well surface, and allowed to crawl off the brush onto the diet. To avoid systematic errors, larvae were not arranged in treatment column order but rather in a rectangular pattern. After every 12 individual larvae, the brush was cleaned using 70% ethanol followed by sterile tap water. The filled plate was sealed with an optically clear adhesive qPCR seal sheet (#AB-1170, Termo Scientific, USA, or #BS3017000, Bioleader, USA), enabling data assessments without the need to open the plate. Four to five holes were carefully made with fine 00-insect pins into the seal per well to facilitate aeration. The plates, housing the larvae, were then incubated in a dark, ventilated incubator at a temperature of 23-25 °C and a relative humidity of 50 to 90% for a period of 5 days. We assessed mortality and stunting larvae within 3 and 5 days. </p> <p>To access the effect and dose-responses of common insecticides on <em>D.v.virgifera</em> adults, artificial diet-overlay bioassays with different doses were performed under controlled, semi-sterile conditions. Each insecticide was prepared in at least six concentrations. Active ingredients as specified on the product labels underwent serial dilutions using sterile tap water. Sterilized tap water was used as untreated control. In detail, each bioassay consisted of 6 polystyrene plates of 6 wells each (Eppendorf® 0030720016). Each treatment was applied to 3 wells of each plate per bioassay. The adult diet for a bioassay had been prepared 1-7days before treatment and adult infestation. The diet was prepared under semi-sterile conditions. The diet was poured out to 5-6 sterile 11 mm Petri dishes. The plates with diet were allowed to dry for up to 15 minutes under laminar flow cabinet then stored at 3 to 5°C overnight.The following day, a core of the diet was initially transferred to each well using flamed iron core-cutter (1 cm diameter) under a laminar flow. A core diet was placed each of the 6 wells of the plates. Approximately 40 µl of the treatments were then applied across the surface of diet core (0.34 cm<sup>3</sup>). The following day, a core of the diet was initially transferred to each well using flamed iron core-cutter (1 cm diameter) under a laminar flow. A core diet was placed each of the 6 wells of the plates. Approximately 40 µl of the treatments were then applied across the surface of diet core (0.34 cm<sup>3</sup>). Adult were subsequently transferred from the rearing cage into the wells of the 6-well plates containing the diet and treatments using a tube aspirator. For ease of transfer, the adults were cooled in a fridge for 4 to 7 minutes. Each well plate received 3 to 4 adults. Plates were sealed and incubated at 23-25<sup>0</sup>C, 50–90% r.h, L: D 12:12. Adult mortality were recorded on days 1,3, 5, 7 of experiment. </p> <p>To allow comparisons between experiments, data were standardized to the data of the corresponding negative control, usually sterilized tap water, as follows: standardized data = 100 × (data in negative control - data in treatment)/maximum (data in control or in treatment). The distributions of the data were investigated using histograms and QQ normal and detrended normal probability. Skewness and kurtosis of residuals was also observed for normality of influences of treatments on eggs, neonates, or adults. Equality of variances was assessed using Levene’s test. Multiple comparisons were performed using the Tukey HSD post hoc test for data with equal variances and the Games-Howell post hoc test for data with unequal variances. For each tested insecticide, linear and logarithmic regression models were fit to the dose-response data. In case of significant linear or logartimic relathionships, doses leading to 50% or 80% of relative effects (ED <sub>50,80</sub>) were calculated. </p> <p>The raw data as well as the standardised data are available as a csv file on zenodo. </p> <p> </p> <p> </p>
Philorhizus margii median lobe of aedeagus Figurre 2 Wrase DW, Assmann T (2008) A new species of Philorhizus Hope, 1838 from Greece (Coleoptera, Carabidae, Lebiini). ZooKeys 3: 1-10. doi: 10.3897/zookeys.3.19
<p>Wrase DW, Assmann T (2008) A new species of <em>Philorhizus </em>Hope, 1838 from Greece (Coleoptera, Carabidae, Lebiini). ZooKeys 3: 1-10. doi: 10.3897/zookeys.3.19</p>
Fig. 3 Habitat of Philorhizus marggii Wrase DW, Assmann T (2008) A new species of Philorhizus Hope, 1838 from Greece (Coleoptera, Carabidae, Lebiini). ZooKeys 3: 1-10. doi: 10.3897/zookeys.3.19
<p>Habitat of Philorhizus marggii<br> Greece: Peloponnese/Taygetos: Profitis Illias, subalpine altitude, 16.V.2007, 2000-2400m a.s.l.</p> <p> </p>
Agonum sordidum, Fig_6 from Assmann et al. (2021) The ground beetle tribe Platynini Bonelli, 1810 (Coleoptera, Carabidae) in the southern Levant: dichotomous and interactive identification tools, ecological traits, and distribution.
<p>New version as *png</p> <p>Agonum sordidum,</p> <p>Fig_6 from Assmann et al. (2021) The ground beetle tribe Platynini Bonelli, 1810 (Coleoptera, Carabidae) in the southern Levant: dichotomous and interactive identification tools, ecological traits, and distribution.</p>
Agonum rugicolle, Fig_5 from Assmann et al. (2021) The ground beetle tribe Platynini Bonelli, 1810 (Coleoptera, Carabidae) in the southern Levant: dichotomous and interactive identification tools, ecological traits, and distribution.
<p>New version as *png</p> <p>Agonum rugicolle,</p> <p>Fig_5 from Assmann et al. (2021) The ground beetle tribe Platynini Bonelli, 1810 (Coleoptera, Carabidae) in the southern Levant: dichotomous and interactive identification tools, ecological traits, and distribution.</p>
Agonum_nigrum, Fig_4 from Assmann et al. (2021) The ground beetle tribe Platynini Bonelli, 1810 (Coleoptera, Carabidae) in the southern Levant: dichotomous and interactive identification tools, ecological traits, and distribution.
<p>New version as *png</p> <p>Agonum_nigrum,</p> <p>Fig_4 from Assmann et al. (2021) The ground beetle tribe Platynini Bonelli, 1810 (Coleoptera, Carabidae) in the southern Levant: dichotomous and interactive identification tools, ecological traits, and distribution.</p>
Agonum_mesostictum, Fig_2 from Assmann et al. (2021) The ground beetle tribe Platynini Bonelli, 1810 (Coleoptera, Carabidae) in the southern Levant: dichotomous and interactive identification tools, ecological traits, and distribution.
<p>New version as *png</p> <p>Agonum_mesostictum,</p> <p>Fig_2 from Assmann et al. (2021) The ground beetle tribe Platynini Bonelli, 1810 (Coleoptera, Carabidae) in the southern Levant: dichotomous and interactive identification tools, ecological traits, and distribution.</p>
Agonum monachum syriacum, Fig_3 from Assmann et al. (2021) The ground beetle tribe Platynini Bonelli, 1810 (Coleoptera, Carabidae) in the southern Levant: dichotomous and interactive identification tools, ecological traits, and distribution.
<p>New version as *png</p> <p>Agonum monachum syriacum,</p> <p>Fig_3 from Assmann et al. (2021) The ground beetle tribe Platynini Bonelli, 1810 (Coleoptera, Carabidae) in the southern Levant: dichotomous and interactive identification tools, ecological traits, and distribution.</p>
Agonum_marginatum, Fig_1 from Assmann et al. (2021) The ground beetle tribe Platynini Bonelli, 1810 (Coleoptera, Carabidae) in the southern Levant: dichotomous and interactive identification tools, ecological traits, and distribution.
<p>New version as *png</p> <p>Agonum_marginatum,</p> <p>Fig_1 from Assmann et al. (2021) The ground beetle tribe Platynini Bonelli, 1810 (Coleoptera, Carabidae) in the southern Levant: dichotomous and interactive identification tools, ecological traits, and distribution.</p> <p> </p>
Ground beetle (Coleoptera:Carabidae) species composition of three forests in the Netherlands
<p>During this research the carabid fauna assemblage of two forests, the Amsterdamse Bos and Purmerendse Bos, was determined. Throughout the forests series (locations within the forests) were chosen to place pitfall traps. Each series consisted of five plastic cups that were dug into the soil in such a way that they were flush with the surface. Each cup was located five meters away from the subsequent one. The cups were filled with formaldehyde (diluted water 1: 10) as conservative and a small amount of soap in order to decrease water tension and thus let the organisms submerge. Afterwards the trap was covered with a wooden plate attached onto the soil with nails to protect it from rain and damage. The plates were covered up with plant material as camouflage. A small opening in between the soil and the plate was left so there was space for soil fauna to crawl into the cup (Picture 1). Because carabids are often dispersed throughout an area in small populations instead of being homologous spread (Raino & Niemelä, 2003) a diversity of locations was chosen. Therefore biotic and abiotic conditions were recorded (soil, light invasion, litter and dominant vegetation) to select the most diverse sites. </p> <p>Nine series in the Amsterdamse Bos and eleven series in the Purmerendse Bos were placed. These forests were sampled for a time span of 63 days. When traps were emptied the formaldehyde was refreshed. After the third time all of the traps were removed and holes filled up with soil. </p> <p>The content of emptied traps was washed with water and afterwards the ground beetles were selected and preserved in 95% ethanol. The ground beetles found were identified by making use of “De Loopkevers van Nederland & Vlaanderen” by Boeken, Desender, Drost, van Gijzen, Koese, Muilwijk, Turin & Vermeulen (2002. </p> <p>For each series the quantity of caught individuals was recorded. From the Eyserbos, data collected by supervisor B. Brugge in the years from 2012 to 2014 was used for analysis; during this research the same catching methods were used but the time scale was different. For four years, five series of pitfall traps were placed for one week halfway of June thus for a total of 28 days. Data of the species composition and the ecological characteristics and classification of the three forests was collected. Following classifications and ecological characters of the species that were used for analysis were documented: the status of the species in the Netherlands, Belgium, Denmark and Luxemburg, the status of the species in the Netherlands, the distribution in the Netherlands, how important the species’ population in the Netherlands is in its distribution in Europe (so called I-species), which type of habitats a species can migrate through to spread to other habitat patches, the classification of the species by Lindroth (1969), the degree of eurytopicity of the species and the flight capabilities of the species. </p> <p>Data obtained can be found in the file:</p> <p><strong>201703-05_groundbeetle_species_composition_Eyserbos_AmsterdamseBos_and_PurmerendseBos.txt</strong></p> <p>The possible inputs for characteristics can be found in the file</p> <p><strong>201706_Legenda_data_groundbeetles_species_composition_Amsterdamse_and_Purmerendse_bos.txt</strong></p>
Supplementary videos for "A second fossil species of the enigmatic rove beetle genus Charhyphus in Eocene Baltic amber, with implications on the morphology of the female genitalia (Coleoptera: Staphylinidae: Phloeocharinae)"
<p><strong>Original figures used in this study:</strong></p> <p>The holotype of <em>Charhyphus serratus </em>sp. nov. and four extant <em>Charhyphus </em>species.</p> <p> </p> <p><strong>Supplementary Videos 1–3:</strong></p> <p><strong>Supplementary Videos 1</strong> <em>Charhyphus serratus </em>sp. nov., 001 DUBC, holotype, habitus, movie of X-ray micro-CT volume renderings.</p> <p><strong>Supplementary Videos 2</strong> <em>Charhyphus serratus </em>sp. nov., 001 DUBC, holotype, habitus, movie of X-ray micro-CT volume renderings using different parameters from Supplementary Videos 1.</p> <p><strong>Supplementary Videos 3</strong> <em>Charhyphus serratus </em>sp. nov., 001 DUBC, holotype, female genitalia, movie of X-ray micro-CT volume renderings.</p>
Coleoptera morphospecies abundance in pitfall traps in sub project 7 in KiLi project
<p>Carabidae identified to species, and other Coleoptera identified to morphospecies by Peter Schüle, Tenebrionidae identified by Dr. Wolfgang Schawaller.</p> <p>Assemblages of ground-dwelling beetles were sampled with pitfall traps8. Ten pitfall traps were evenly spaced along two 50 m transects, with a distance of 10 m between individual traps and 20 m between transects. Pitfall traps were filled with 100-200 ml solution of equal parts of ethylenglycol and water with a drop of liquid soap to break the surface tension. The traps were placed on the sampling sites in June 2012 and collected after seven days. As the number of individuals collected in ten traps was very high and all individuals could not be analyzed in time, for the present analysis, we processed only three traps from each study site. Ground-dwelling beetles were sorted to morphospecies level, and where possible, to species.</p> <div> <p>The KiLi project (2010-2018) is a German Science Foundation (DFG) funded research unit (DFG research unit FOR1246) that focuses on biodiversity and ecosystem processes along altitudinal and disturbance gradients on Mt. Kilimanjaro (Tanzania, Africa), capitalizing on its world-wide unique range of climatic and vegetation zones. The research unit comprises 2 central projects and 7 subprojects from various disciplines. On a total of 60 study sites in both natural and human-disturbed ecosystems biodiversity (e.g. plants, soil arthropods, ants, bees, frogs, lizards, bats, birds), related ecosystem processes (decomposition, seed dispersal, pollination, herbivory, predation), and biogeochemical processes and properties of ecosystems (climate, soil properties and nutrient status, regulation of water and carbon fluxes, trace gas emissions, primary productivity, functional diversity) are analyzed.</p> </div>
Coleoptera Carabidae body size in pitfall traps in sub project 7 in KiLi project
<p>There are still some unsolved qustions regarding plot level information, but means per Carabid species are available. Abacetus spec., Afrotarus kilimanus, Neosipelus sp. 1 and sp 2., Tyronia lateralis and Tyronia spec. nov. - measurements are available, but without information for the plot.</p> <p>Assemblages of ground-dwelling beetles were sampled with pitfall traps8. Ten pitfall traps were evenly spaced along two 50 m transects, with a distance of 10 m between individual traps and 20 m between transects. Pitfall traps were filled with 100-200 ml solution of equal parts of ethylenglycol and water with a drop of liquid soap to break the surface tension. The traps were placed on the sampling sites in June 2012 and collected after seven days. As the number of individuals collected in ten traps was very high and all individuals could not be analyzed in time, for the present analysis, we processed only three traps from each study site. Ground-dwelling beetles were sorted to morphospecies level, and where possible, to species.</p> <p>The KiLi project (2010-2018) is a German Science Foundation (DFG) funded research unit (DFG research unit FOR1246) that focuses on biodiversity and ecosystem processes along altitudinal and disturbance gradients on Mt. Kilimanjaro (Tanzania, Africa), capitalizing on its world-wide unique range of climatic and vegetation zones. The research unit comprises 2 central projects and 7 subprojects from various disciplines. On a total of 60 study sites in both natural and human-disturbed ecosystems biodiversity (e.g. plants, soil arthropods, ants, bees, frogs, lizards, bats, birds), related ecosystem processes (decomposition, seed dispersal, pollination, herbivory, predation), and biogeochemical processes and properties of ecosystems (climate, soil properties and nutrient status, regulation of water and carbon fluxes, trace gas emissions, primary productivity, functional diversity) are analyzed.</p>
Staines & Staines 2021: The Geadephaga (Coleoptera: Carabidae and Rhysodidae) of SERC (repackaging of occurrences published by the NEON Biorepository Data Portal)
Linked records of Carabidae and Rhysodidae specimens from the SERC site, collected between 2015-2018, included in the following publication: Staines, C.L. & Staines, S. L. The Geadephaga (Coleoptera: Carabidae and Rhysodidae) of the Smithsonian Environmental Research Center, Maryland. 2021. Banisteria 55: 75-100. Original abstract: "An inventory of the Geadephaga (Coleoptera) at the Smithsonian Environmental Research Center, Anne Arundel County, Maryland is being conducted. Pitfall traps were placed and monitored from 2015 to 2018. From 2017 to 2020 directed collecting efforts were made to document the Geadephaga of the facility. A total of 111 Geadephaga species was collected: Carabidae - 110, Rhysodidae - 1." Research article available for download: https://virginianaturalhistorysociety.com/banisteria/pdf-files/ban55/Staines_SERC_Geadephaga.pdf
Figures 9-15 in A new species of Malthonea Thomson, 1864 (Coleoptera, Cerambycidae, Lamiinae) from Ecuador
Figures 9-15. (9) Malthoneaminima Martins & Galileo, 1995, holotype male, dorsalview (scanningof the photographsubmitted topublication – formerpersonal collectionof Ubirajara R. Martins). (10) Prymnoptetyxglaucina Thomson, 1868, holotype, dorsalview. (11) syntypesof Malthonearuficornis Belon, 1903, dorsalview (by Jesus Santiago Moure); (12) Blabia aurescens Breuning, 1966, holotype, dorsalview (by Eugenio Nearns; alsoavailable at http://cerambycids.com/longicornid/ default.asp?a=home); (13‑15) holotypeof Ptericoptusguttatus Kirsch, 1889 (by Olaf Jaeger): (13) dorsalhabitus; (14) lateralhabitus; (15) labels.
Supplementary materials (Allometry and fighting behaviour of a dimorphic stag beetle Cyclommatus miniszechi (Coleoptera: Lucanidae))
<p><strong>Supplementary Materials:</strong></p> <p><strong>Table S1.</strong> The morphological measurements of males of <em>Cyclommatus mniszechi</em> used for allometry analyses.</p> <p><strong>Table S2. </strong>The behavioural sequence data used for sequential analyses of size-matched contests in major males of <em>Cyclommatus mniszechi</em>.</p> <p><strong>Table S3. </strong>The behavioural sequence data used for sequential analyses of size-matched contests in minor males of <em>Cyclommatus mniszechi</em>.</p> <p><strong>Video S1.</strong> The behavioural sequence of males of <em>Cyclommatus mniszechi</em> in fighting contests under the laboratory setups. One of the opponents walked to the other and touched it (00:07), and then both of them displayed ‘defensive posture’ (00:08) after ‘touch’. Once both individuals approached each other, they accelerated antennation and raised their mandibles and prothoracic parts. The contest then progressed into ‘body raising’ (00:14). They then performed ‘attack’ and ‘push’ to each other several times and then escalated to ‘tussle’ (00:20) and interlocked their mandibles until one of the contestants was clamped (‘clamp1’) in the air by the other for a second and flipped (00:50). The winner dropped the loser and kept attacking and pushing the loser while the loser retreated and moved backwards (00:51).</p>
Fig. 2 in Taxonomic revision of the South American subgenus Canthon (Peltecanthon) Pereira, 1953 (Coleoptera: Scarabaeidae: Scarabaeinae: Deltochilini)
Fig. 2. Canthon (Peltecanthon) staigi (Pereira, 1953), ♂, Rio de Janeiro, Restinga da Marambaia (CEMT). A. Parameres lateral view. B. Parameres dorsal view. C. Internal sac ventral view. D. Internal sac dorsal view. Abbreviations: see Material and methods.
Fig. 7 in Taxonomic revision of the South American subgenus Canthon (Peltecanthon) Pereira, 1953 (Coleoptera: Scarabaeidae: Scarabaeinae: Deltochilini)
Fig. 7. Canthon (Peltecanthon) terciae sp. nov., paratype, ♂, Rio Grande do Norte, Baia Formosa, Mata Estrela (CEMT). A. Dorsal habitus. B. Ventral surface of meso- and metafemora. C. Transverse carina in the hypomeron. D. Carina in the margin between pygidium and propygidium. E. Male protibial spur. F. Paratype, ♀, Rio Grande do Norte, Baia Formosa, Mata Estrela (CEMT), protibial spur.
Fig. 1 in Taxonomic revision of the South American subgenus Canthon (Peltecanthon) Pereira, 1953 (Coleoptera: Scarabaeidae: Scarabaeinae: Deltochilini)
Fig. 1. Canthon (Peltecanthon) staigi (Pereira, 1953), ♂, São Paulo, Bertioga (CEMT). A. Dorsal habitus. B. Ventral surface of meso- and metafemora. C. Transverse carina in the hypomeron. D. Carina in the margin between pygidium and propygidium. E. Male protibial spur. F. Female protibial spur, São Paulo, Caraguatatuba, P.E. Serra do Mar (CEMT).
Fig. 7 in Eight new species of Ptenidiini and Discheramocephalini (Coleoptera: Ptiliidae) from Ecuador
Fig. 7. Discheramocephalus nigerrimus sp. nov. A. Habitus. B. Pronotum ×725. C. Side of head ×2220. D. Meso- and metaventra ×830. E. Mesoventrite keel and metaventrite projection between mesocoxae ×830.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.