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170 results for “community metabarcoding”
COI metabarcoding data from arthropod pollinator communities in burned and unburned sites of California
<p>Novel wildfire regimes are rapidly changing global ecosystems and pose significant challenges for biodiversity conservation and ecosystem management. In this study, we used DNA metabarcoding to assess the response of arthropod pollinator communities to large-scale wildfires across diverse habitat types in California. We sampled six reserves within the University of California Natural Reserve System (UCNRS), each of which was partially burned in the 2020 Lightning Complex wildfires in California. Using yellow pan traps to target pollinators, we collected arthropods from burned and unburned sites across multiple habitat types including oak woodland, redwood, scrub, chamise, grassland, forest, and serpentine habitats. We found no significant difference in alpha diversity values between burned and unburned sites; instead, seasonal variations played a significant role in arthropod community dynamics, with the emergence of plant species in Spring promoting increased pollinator richness at all sites. When comparing all sites, we found that burn status was not a significant grouping factor. Instead, compositional differences were largely explained by geographic differences, with distinct communities within each reserve. Within a geographic area, the response of arthropods to fire was dependent on habitat type. While communities in grasslands and oak woodlands exhibited recovery following burn, scrublands experienced substantial changes in community composition. Our study highlights the importance of examining community responses to wildfires across broad spatial scales and diverse habitat types. By understanding the nuanced dynamics of arthropod communities in response to fire disturbances, we can develop effective conservation strategies that promote resilience and maintain biodiversity in the face of increasing wildfire frequency and severity driven by climate change.</p>
Semi‐quantitative metabarcoding reveals how climate shapes arthropod community assembly along elevation gradients on Hawaii Island
<p>Spatial variation in climatic conditions along elevation gradients provides an important backdrop by which communities assemble and diversify. Lowland habitats tend to be connected through time, whereas highlands can be continuously or periodically isolated, conditions that have been hypothesized to promote high levels of species endemism. This tendency is expected to be accentuated among taxa that show niche conservatism within a given climatic envelope. While species distribution modeling approaches have allowed extensive exploration of niche conservatism among target taxa, a broad understanding of the phenomenon requires sampling of entire communities. Species-rich groups such as arthropods are ideal case studies for understanding ecological and biodiversity dynamics along elevational gradients given their important functional role in many ecosystems, but community-level studies have been limited due to their tremendous diversity. Here, we develop a novel semi-quantitative metabarcoding approach that combines specimen counts and size-sorting to characterize arthropod community-level diversity patterns along two elevational gradients across two volcanoes on the island of Hawai`i. We find that arthropod communities between the two transects become increasingly distinct compositionally at higher elevations. Resistance surface approaches suggest that climatic differences between sampling localities are an important driver in shaping beta-diversity patterns, though the relative importance of climate varies across taxonomic groups. Nevertheless, the climatic niche position of OTUs between transects was highly correlated, suggesting that climatic filters shape the colonization between adjacent volcanoes. Taken together, our results highlight climatic niche conservatism as an important factor shaping ecological assembly along elevational gradients and suggest topographic complexity as an important driver of diversification.</p>
Data from: Metabarcoding of soil environmental DNA replicates plant community variation but not specificity
<blockquote> <p>While metabarcoding of plant DNA from their environment is an exciting method that can supplement inventorying of live plant species, the accuracy and specificity has yet to be fully assessed over complex continuous landscapes. In this work, we evaluate plant community profiles produced via metabarcoding of soil by comparing them to a morphological survey. We assessed plant communities by metabarcoding of soil DNA in 130 sites along ecological gradients (nutrients, succession, moisture) in Denmark using chloroplast <i>trn</i>L region (10-143 bp) primer set and compared the resulting communities to communities produced with a longer nuclear ITS2 region (~216 bp) and a morphological survey. We found that the community variation observed within the morphological survey was well represented by molecular surveys, with significant correlation with both community composition and richness using both primer sets. While the majority of the ITS2 sequences could be assigned to species (over 80%), we had less success with the <i>trn</i>L sequences (70%), which was only possible after restricting the reference database to local species. We conclude that the community profiles produced by metabarcoding can be highly effective in performing large-scale macroecological studies. However, the discovery rates and taxonomic assignments produced via metabarcoding remained inferior to morphological surveys, but manual curation of databases improves the <i>specificity</i> of assignments made by the <i>trn</i>L primers, and improves the <i>accuracy</i> of the assignments made with the ITS2 primers. Finally, we suggest that a greater percentage of named diversity would be recovered by increasing soil sampling with the use of additional universal primer sets.</p> </blockquote>
BeeDNA: microfluidic environmental DNA metabarcoding as a tool for connecting plant and pollinator communities
<p><strong>Data repository accompanying the paper 'BeeDNA: microfluidic environmental DNA metabarcoding as a tool for connecting plant and pollinator communities' by Harper et al. (2021).</strong></p> <p><br> <strong>1_Raw_Data.zip</strong><br> This zipped folder contains the raw sequence data (sorted by primer set and demultiplexed) for both sequencing runs (2019-10-24 and 2019-11-11). To decompress each file, run: </p> <pre><code>tar -xvf filename.bz2</code></pre> <p>This will create a folder for each primer set containing the raw reads for each sample/control.</p> <p><br> <strong>2_Anacapa_Bioinformatic_Processing.zip</strong></p> <p>This zipped folder contains all files needed to perform bioinformatic processing with Anacapa. Please process sequence data belonging to each primer set individually (i.e. do not process sequence data belonging to different primer sets together).</p> <p><br> <strong>3_metaBEAT_Bioinformatic_Processing.zip </strong></p> <p>This zipped folder contains the scripts and files needed to perform bioinformatic processing with metaBEAT. Before running the scripts, move the raw reads for each sample belonging to each primer set into the dedicated folder within metaBEAT_Bioinformatic_Processing, e.g. all .fastq files in Raw_Data > BF1_BR1 should be moved to metaBEAT_Bioinformatic_Processing > BF1-BR1 > raw_reads.</p> <p>To run metaBEAT, you will have to install Docker on your computer. Docker is compatible with all major operating systems, but see the Docker documentation for details. On Ubuntu, installing Docker should be as easy as:</p> <pre><code>sudo apt-get install docker.io</code></pre> <p>Once Docker is installed, you can enter the environment by typing:</p> <pre><code>sudo docker run -i -t --net=host --name metaBEAT -v $(pwd):/home/working chrishah/metabeat /bin/bash</code></pre> <p>This will download the metaBEAT image (if not yet present on your computer) and enter the 'container', i.e. the self contained environment (NB: sudo may be necessary in some cases). With the above command, the container's directory /home/working will be mounted to your current working directory (as instructed by $(pwd)). In other words, anything you do in the container's /home/working directory will be synced with your current working directory on your local machine.</p> <p>Please process sequence data belonging to each primer set individually (i.e. do not process sequence data belonging to different primer sets together). An example of expected outputs can be seen in the Jupyter Notebook for the BF1/BR1 primer set from the 2019-11-11 sequencing run.</p> <p><br> <strong>4_Illinois_Invert_Reference_Database.zip</strong></p> <p>This zipped folder contains all files that were used to generate the custom COI and 16S reference databases for invertebrates that occur in Illinois, U.S. You will need to have metaBEAT installed (see above) before you try to run any Jupyter Notebooks (.ipynb files).</p> <p><br> <strong>5_ecoPCR.zip</strong></p> <p>This zipped folder contains all files used to perform ecoPCR for each primer set evaluated for microfluidic eDNA metabarcoding. You will need to <a href="https://git.metabarcoding.org/obitools/ecopcr/wikis/home">install ecoPCR</a> before running any shell scripts.</p> <p><br> <strong>6_Tidied_Data.zip</strong></p> <p>This zipped folder contains the taxonomically assigned data for both sequencing runs produced by metaBEAT and Anacapa. These were copied over from the folders 2_Anacapa_Bioinformatic_Processing and 3_metaBEAT_Bioinformatic_Processing and rearranged into a more logical order. These files are used as the input for data analysis using R.</p> <p><br> <strong>7_Data_Analysis.zip</strong></p> <p>This zipped folder contains all scripts and metadata required to summarise and statistically analyse data in R.</p> <p> </p> <p><strong>Please contact Dr Lynsey Harper (lynsey.harper2@gmail.com) or Dr Mark Davis (davis63@illinois.edu) if you encounter any issues!</strong></p>
Data from: Metabarcoding of trap nests reveals differential impact of urbanization on cavity-nesting bee and wasp communities
<p><span>Urbanization is affecting arthropod communities worldwide, for example by changing the availability of food resources. However, the strength and direction of a community's response are species-specific and depend on the species' trophic level. Here, we investigated interacting species at different trophic levels in nests of cavity-nesting bees and wasps along two urbanization gradients in four German cities using trap nests. We analyzed bee and wasp diversity and their trophic interaction partners by metabarcoding the DNA of bee pollen and preyed arthropods found in wasp nests. We found that the pollen richness increased with increasing distance from city centers and at sites characterized by a high percentage of impervious and developed surfaces, while the richness of pollinators was unaffected by urbanization. In contrast, species richness of wasps, but not their arthropod prey, was highest at sites with low levels of urbanization. However, the community structure of wasp prey changed with urbanization at both local and regional scales. Throughout the study area, the community of wasps consisted of specialists, while bee species were generalists. Our results suggest that Hymenoptera and their food resources are negatively affected by increasing urbanization. However, to understand the distribution patterns of both, wasps and bees in urban settings other factors besides food availability should be considered.</span></p>
Data from: Using environmental DNA metabarcoding to monitor fish communities in small rivers and large brooks: Insights on the spatial scale of information
<p><span>Monitoring fish communities is central to the evaluation of ecological health of rivers. Not only presence/absence of species is important to assess, but also the species composition of local fish assemblages is a crucial parameter. Lotic fish communities are traditionally monitored via electrofishing, characterized by a known limited efficiency and high survey costs. The use of environmental DNA-based analyses could serve as a non-destructive alternative, but this approach requires further insights in practical sampling schemes incorporating transport and dilution of the eDNA fragments; as well as optimization of molecular detection in terms of predictive power and quality assurance. By introducing fifteen species known to occur in Belgian waters via a controlled cage experiment, we aim to extend the knowledge on streamreach of eDNA in small rivers and large brooks, as laid out in the European Water Framework Directive's water typology. Introducing fish communities in two transects of a species poor river characterized by contrasting river discharge rates, we found strong and significant correlations between the eDNA relative abundances and the relative biomass per species in the cage community. Despite a decreasing correlation over distance, the underlying community composition remained stable over a distance of 300 m up to 1 km downstream of the cages, depending on the river discharge rate. Such decrease in similarity between relative source biomass and the corresponding eDNA-based community profile with increasing distance downstream from the source, can partly be attributed to variation in species-specific eDNA persistence. Our findings offer novel insights on eDNA behaviour and characterization of riverine fish communities. We conclude that water sampled from a relatively small river offers an adequate snapshot of the total fish community composition occurring within an upstream perimeter ranging between 300 and 1000 meters. The potential application for other river systems is discussed in this study. </span></p>
Data from: Reduced sampling intensity through key sampling site selection for optimal characterization of riverine fish communities by eDNA metabarcoding
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Long- and short-read metabarcoding technologies reveal similar spatio-temporal structures in fungal communities
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Data from: Metabarcoding of trap nests reveals differential impact of urbanization on cavity-nesting bee and wasp communities
Open the record for dataset details and reuse information.
COI metabarcoding data from arthropod pollinator communities in burned and unburned sites of California
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Data from: Comparative analysis of environmental DNA metabarcoding and spectro-fluorescence for phytoplankton community assessments
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Data from: Using environmental DNA metabarcoding to monitor fish communities in small rivers and large brooks: Insights on the spatial scale of information
Open the record for dataset details and reuse information.
Semi‐quantitative metabarcoding reveals how climate shapes arthropod community assembly along elevation gradients on Hawaii Island
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Data from: Metabarcoding of soil environmental DNA replicates plant community variation but not specificity
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A novel metabarcoding strategy for studying nematode communities
<p>Nematodes are most abundant soil metazoa. How, the ecology of this group of organism remains poorly elucidated, partly due to the lack of reliable and validated sequencing strategies. In this study, we tested four primer sets for amplicon sequencing: JB3/JB5, SSU_04F/SSU_22R, Nemf/18Sr2b from earlier studies; and MMSF/MMSR, a newly developed primer set from this study. In order to test these primer sets, we used 22 samples of individual nematode species, 20 mock communities, 20 soil samples, 20 mock communities in soil, and 4 root/rhizosphere soil samples. We successfully amplified the target regions (COI gene; V1-V2, V4-V8 region of 18S rRNA gene) from these 86 DNA samples and sequenced the amplicons on an Illumina MiSeq sequencing platform. We found that the MMSF/MMSR and Nemf/18Sr2b were very efficient in detecting soil nematode compared to JB and SSU primer sets based on annotation of sequence reads at genus and in some cases at species level. Therefore, these primer sets could be used to study nematode communities in agricultural environments.</p>
Data from: Using full-length metabarcoding and DNA barcoding to infer community assembly for speciose taxonomic groups: a case study
<p>How insect communities are assembled in nature remains largely unknown. In particular, whether habitat filtering or competition serves as the main mechanism in forming insect communities is rarely subject to an in-depth investigation. One bottleneck lies in the difficulty of species identification when dealing with a large number of diverse insects. However, High-Throughput Sequencing (HTS) technology coupled with classic DNA barcoding offers a great opportunity to infer community assembly for this speciose group. In this study, using 13,909 full-length barcodes obtained by Sanger sequencing or the SOAPBarcode metabarcoding method, we showed that competition was the main assembly mechanism for the moth communities studied in temperate forests of China. The two sequencing methods showed highly consistent results with regards to both diversity composition and community assembly mechanism. Significant phylogenetic signals and structure suggested that the focal moth communities were the result of the non-neutral assembly process, which was further confirmed by results of neutral assembly test that accounted for immigration and speciation rates. In conclusion, HTS coupled with a well-curated DNA barcode library can facilitate community assembly inferences, especially for speciose taxonomic groups.</p>
Data from: Biodiversity assessment of tropical shelf eukaryotic communities via pelagic eDNA metabarcoding
<p>Our understanding of marine communities and their functions in an ecosystem relies on the ability to detect and monitor species distributions and abundances. Currently, the use of environmental DNA (eDNA) metabarcoding is increasingly being applied for the rapid assessment and monitoring of aquatic species. Most eDNA metabarcoding studies have either focused on the simultaneous identification of a few specific taxa/groups or have been limited in geographical scope. Here we employed eDNA metabarcoding to compare beta diversity patterns of complex pelagic marine communities in tropical coastal shelf habitats spanning the whole Caribbean Sea. We screened 68 water samples using a universal eukaryotic COI barcode region and detected highly diverse communities, which varied significantly among locations, and proved good descriptors of habitat type and environmental conditions. Less than 15% of eukaryotic taxa were assigned to metazoans, most DNA sequences belonged to a variety of planktonic 'protists', with over 50% of taxa unassigned at the phylum level, suggesting that the sampled communities host an astonishing amount of micro-eukaryotic diversity yet undescribed or absent from COI reference databases. Although such a predominance of micro-eukaryotes severely reduces the efficiency of universal COI markers to investigate vertebrate and other metazoans from aqueous eDNA, the study contributes to the advancement of rapid biomonitoring methods, and brings us closer to a full inventory of extant marine biodiversity.</p>
Community metabarcoding reveals the relative role of environmental filtering and spatial processes in metacommunity dynamics of soil microarthropods across a mosaic of montane forests
<p><span><span><span><span><span><span><span><span><span><span><span>Disentangling the relative role of environmental filtering and spatial processes in driving metacommunity structure across mountainous regions remains challenging, as the way we quantify spatial connectivity in topographically and environmentally heterogeneous landscapes can influence our perception of which process predominates. More empirical datasets are required to account for taxon- and context-dependency but relevant research in understudied areas is often compromised by the taxonomic impediment<span><span>. </span></span>We here employed haplotype-level community DNA metabarcoding, enabled by stringent filtering of Amplicon Sequence Variants (ASVs), to characterize metacommunity structure of soil microarthropod assemblages across a mosaic of five forest habitats on the Troodos mountain range in Cyprus. We found similar β diversity patterns at ASV and species (OTU, Operational Taxonomic Unit) levels, which pointed to a primary role of habitat filtering resulting in the existence of largely distinct metacommunities linked to different forest types. Within-habitat turnover was correlated to topoclimatic heterogeneity, again emphasizing the role of environmental filtering. However, when integrating landscape matrix information for the highly fragmented <i>Quercus alnifolia</i> habitat, we also detected a major role of spatial isolation determined by patch connectivity, indicating that stochastic and niche-based processes synergistically govern community assembly. Alpha diversity patterns varied between ASV and OTU levels, with OTU richness decreasing with elevation and ASV richness following a longitudinal gradient, potentially reflecting a decline of genetic diversity eastwards due to historical pressures. Our study demonstrates the utility of haplotype-level community metabarcoding for characterising metacommunity structure of complex assemblages and improving our understanding of biodiversity dynamics across mountainous landscapes worldwide.</span></span></span></span></span></span></span></span></span></span></span></p>
Metabarcoding of canopy arthropods reveals negative impacts of forestry insecticides on community structure across multiple taxa
<p>1. Insecticides used to combat outbreaks of forest defoliators can adversely affect non-target arthropods. Forest use insecticides typically suppress Lepidoptera larvae which are the keystone of the canopy community of deciduous oak forests. The abrupt removal of this dominant component of the food web could have far-reaching implications for forest ecosystems, yet it is rarely investigated in practice owing to several methodological shortcomings. The taxonomic impediment and the biased nature of arthropod sampling techniques particularly impede the assessment of insecticide impacts on diverse communities.</p> <p>2. To tackle this issue, we propose an experimental approach combining pyrethrum knockdown sampling and species determination via DNA metabarcoding, using community subsampling to derive estimates of species abundances. We applied this protocol to investigate the short-term effects of the insecticides diflubenzuron (DFB) or <i>Bacillus thuringiensis</i> var. <i>kurstaki</i> (BTK) on canopy-dwelling arthropod communities in German oak woodlands.</p> <p>3. Our approach allowed us to include most of the detected diversity and integrate species abundances in our analyses. By classifying arthropod species into assemblages based on their expected sensitivity rather than coarse taxonomic groupings, we could unveil substantial effects of DFB across multiple taxa five weeks after application.</p> <p>4. Although strong effects on single species appear related to direct toxicity, substantial impacts of DFB on parasitoids and xylophagous beetles suggest that anti-defoliator treatments can have previously unsuspected indirect effects on some components of forest arthropod communities. The impacts of BTK on community structure were consistent with but much weaker than that of DFB.</p> <p>5. <i>Synthesis and applications</i>. Comparing diversity patterns in the arthropod communities of sprayed and unsprayed oak canopies, our results show that selective insecticides can alter species diversity in presumably non-sensitive taxa. Even though the ecological significance of these impacts has yet to be assessed in an operational setting, their existence calls for increased regulatory scrutiny on indirect effects. As community approaches become more attainable with the rapid development of DNA metabarcoding, we suggest the inclusion of community level endpoints as regulatory requirements for the approval of forest use insecticides.</p>
Do pseudogenes pose a problem for metabarcoding marine animal communities?
<p>Because DNA metabarcoding typically employs sequence diversity among mitochondrial amplicons to estimate species composition, nuclear mitochondrial pseudogenes (NUMTs) can inflate diversity. This study quantifies the incidence and attributes of NUMTs derived from the 658 bp barcode region of cytochrome c oxidase I (COI) in 156 marine animal genomes. NUMTs were examined to ascertain if they could be recognized by their possession of indels or stop codons. In total, 309 NUMTs 150 bp were detected, with an average of 1.98 per species (range = 0–33) and a mean length of 391 bp 200 bp. Among this total, 75 (23.4%) lacked indels or stop codons. NUMTs appear to pose the greatest interpretational risk when short (< 313 bp) amplicons are used, such as in eDNA studies, dietary analyses, or processed fish identification. Employing the standard amplicon length (313 bp) for marine metabarcoding, NUMTs could potentially inflate the OTU count by 21% above the true species count while also raising intraspecific variation at COI by 15%. However, when both amplicon length and position are considered, inflation in OTU counts and in barcode variation were just 9% and 10%, respectively, suggesting NUMTs will not seriously distort biodiversity assessments. There was a weak positive correlation between genome size and NUMT count but no variation among phyla or trophic groups. Until bioinformatic advances improve NUMT detection, the best defense involves targeting long amplicons and developing reference databases that include both mitochondrial sequences and their NUMT derivatives. </p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.