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202
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Dataset results
202 results for “contractility”
Myosin turnover controls actomyosin contractile instability
<p>Simulation and experimental data related to the preprint "Myosin turnover controls actomyosin contractile instability" (https://www.biorxiv.org/content/10.1101/2021.03.18.436017). </p>
Mechanistic Drivers of Acute PAPE Responsiveness: Muscle Architecture, Contractile Kinetics, and Excitability in a Randomized Controlled Trial
ClinicalTrials.gov study NCT06982937. IPD Sharing: NO. Countries: 1. Publications: 1.
Data rom: Stiffness anisotropy coordinates supracellular contractility driving long-range myotube-ECM alignment
<p>The ability of cells to organize into tissues with proper structure and function requires the effective coordination of proliferation, migration, polarization, and differentiation across length scales. Skeletal muscle is innately anisotropic; however, few biomaterials can emulate mechanical anisotropy to determine its influence on tissue patterning without introducing confounding topography. Here, we demonstrate that substrate stiffness anisotropy coordinates contractility-driven collective cellular dynamics resulting in C2C12 myotube alignment over millimeter-scale distances. When cultured on mechanically anisotropic liquid crystalline polymer networks (LCNs) lacking topography, C2C12 myoblasts collectively polarize in the stiffest direction. Cellular coordination is amplified through reciprocal cell-ECM dynamics that emerge during fusion, driving global myotube-ECM ordering. Conversely, myotube alignment was restricted to small local domains with no directional preference on mechanically isotropic LCNs of the same chemical formulation. These findings provide valuable insights for designing biomaterials that mimic anisotropic microenvironments and underscore the significance of stiffness anisotropy in orchestrating tissue morphogenesis.</p>
Colonic contractility recordings following spinal cord injury in mice with dietary fiber interventions
<p>Here, we performed colonic contractility measurements in mice following spinal cord injury, with or without a dietary fiber intervention at 2 weeks post-injury. Provided here are is a .zip file containing the raw ABF measurement files necessary for analysis of the frequency and amplitude of contractions. <span>Breifly, data were acquired using AxoClamp 900A (Axon Instruments). Each tissue segment was recorded for at least 30min in 5min gap-free files. 15min of stable recordings were selected from the middle of each recording session for analysis. Using Clampfit software (Molecular Devices, RRID:SCR_011323), data files were filtered at 300Hz (Bessel 8-pole) and reduced by a factor of 100. Files were concatenated and the baseline was adjusted based on overall slope. Amplitude was calculated by subtracting the minimum value of the whole trace from the value of the peak being assessed. </span></p>
Piezo2 in bladder-innervating sensory neurons contributes to regulation of contractile activity in cystitis
<p>Overactivity of the urinary bladder constitutes one of the main symptoms in various lower urinary tract disorders and it significantly deteriorates quality of life. The crucial role of mechanotransduction in bladder-innervating unmyelinated C-type primary sensory nerve fibres in initiating the voiding reflex in the overactive bladder has been well established. However, the molecular identity of the mechanotransducer(s) in bladder-innervating C-type sensory neurons is still elusive. The mechanosensitive ion channel Piezo2 in primary sensory neurons has been implicated in various physiological and pathological functions including proprioception, the detection of low and high threshold mechanical stimuli in the skin, and the development of mechanical hypersensitivity in peripheral pathologies. Here, we investigated whether or not Piezo2 expressed in primary sensory neurons could contribute to bladder overactivity in cystitis. Characterisation of Piezo2 expression revealed that about 1/5 of primary sensory neurons express this ion channel and at least half of the Piezo2-expressing cells express various markers for unmyelinated C-type sensory neurons. Further, we found that Piezo2 is expressed predominantly in lamina I and II of the spinal cord, where C-type sensory neurons terminate. We disclosed that Piezo2 is expressed in sensory neuron terminals innervating peripheral tissues including the bladder wall and noxious but not innocuous pressure in the urinary bladder activates Piezo2-expressing primary sensory neurons. Finally, we show that instillation of GsMTx that blocks Piezo2 significantly reduces overactivity in the inflamed but not in the naive urinary bladder. Together these findings indicate that Piezo2 in bladder-innervating unmyelinated C-type neurons contributes to the detection of noxious pressure and regulation of contractile activity in cystitis.</p>
Mitochondrial MICOS complex genes, implicated in hypoplastic left heart syndrome, maintain cardiac contractility and actomyosin integrity
<p>Hypoplastic left heart syndrome (HLHS) is a severe congenital heart disease (CHD) with a likely oligogenic etiology, but our understanding of the genetic complexities and pathogenic mechanisms leading to HLHS is limited. We therefore performed whole genome sequencing (WGS) on a large cohort of HLHS patients and their families to identify candidate genes that were then tested in <em>Drosophila</em> heart model for functional and structural requirements. Bioinformatic analysis of WGS data from an index family comprised of a HLHS proband born to consanguineous parents and postulated to have a homozygous recessive disease etiology, prioritized 9 candidate genes with rare, predicted damaging homozygous variants. Of the candidate HLHS gene homologs tested, cardiac-specific knockdown (KD) of mitochondrial MICOS complex subunit dCHCHD3/6 resulted in drastically compromised heart contractility, diminished levels of sarcomeric actin and myosin, reduced cardiac ATP levels, and mitochondrial fission-fusion defects. Interestingly, these heart defects were similar to those inflicted by cardiac KD of ATP synthase subunits of the electron transport chain (ETC), consistent with the MICOS complex's role in maintaining cristae morphology and ETC complex assembly. Analysis of 183 genomes of HLHS patient-parent trios revealed five additional HLHS probands with rare, predicted damaging variants in CHCHD3 or CHCHD6. Hypothesizing an oligogenic basis for HLHS, we tested 60 additional prioritized candidate genes in these cases for genetic interactions with CHCHD3/6 in sensitized fly hearts. Moderate KD of CHCHD3/6 in combination with Cdk12 (activator of RNA polymerase II), RNF149 (E3 ubiquitin ligase), or SPTBN1 (scaffolding protein) caused synergistic heart defects, suggesting the potential involvement of a diverse set of pathways in HLHS. Further elucidation of novel candidate genes and genetic interactions of potentially-disease-contributing pathways is expected to lead to a better understanding of HLHS and other CHDs.</p>
Arrythmia Burden in Cardiac Contractility Modulation (CCM)
ClinicalTrials.gov study NCT05704426. IPD Sharing: NO. Countries: 1. Publications: 7.
Evaluation of Gallbladder Contractility Using Both CCK and Milk Consecutively
ClinicalTrials.gov study NCT02748525. IPD Sharing: NO. Countries: 1. Publications: 6.
COSMIC-HF - Chronic Oral Study of Myosin Activation to Increase Contractility in Heart Failure
ClinicalTrials.gov study NCT01786512. IPD Sharing: Not stated. Countries: 13. Publications: 4.
Data rom: Stiffness anisotropy coordinates supracellular contractility driving long-range myotube-ECM alignment
Open the record for dataset details and reuse information.
Mitochondrial MICOS complex genes, implicated in hypoplastic left heart syndrome, maintain cardiac contractility and actomyosin integrity
Open the record for dataset details and reuse information.
Tetanus-driven biohybrid multi-joint robots powered by muscle rings with enhanced contractile force
Open the record for dataset details and reuse information.
Right ventricular contractility and load in HIV associated pulmonary hypertension
<p class="Body"><span><span><span><span><span><span><span><span><span><span><span>Background: People living with human immunodeficiency virus (PLWH) are at risk of developing pulmonary hypertension (PH) and right ventricular (RV) dysfunction, but understanding of the relationship of RV function to afterload (RV-PA coupling) is limited. We evaluated the clinical and hemodynamic characteristics of human immunodeficiency virus (HIV)-associated PH.</span></span></span></span></span></span></span></span></span></span></span></p> <p class="Body"><span><span><span><span><span><span><span><span><span><span><span>Methods: We performed a retrospective review of patients with a diagnosis of HIV undergoing right heart catheterization (RHC) from 2000-2016 in a tertiary care center. Inclusion criteria were diagnosis of HIV, age ≥ 18 years and availability of RHC data. PH was classified as either pulmonary arterial hypertension (PAH; mean pulmonary arterial pressure [mPAP] ≥ 25mmHg with pulmonary artery wedge pressure [PAWP] ≤ 15mmHg) or pulmonary venous hypertension (PVH; mPAP ≥ 25mmHg with PAWP > 15). We collected demographics, CD4 cell count, HIV viral load, RHC and echocardiographic data. The single beat method was used to calculate RV-PA coupling from RHC.</span></span></span></span></span></span></span></span></span></span></span></p> <p class="Body"><span><span><span><span><span><span><span><span><span><span><span>Results: Sixty-two PLWH with a clinical likelihood for PH underwent RHC. Thirty-two (52%) met PH criteria (15 with PAH, 17 with PVH). Average time from diagnosis of HIV to diagnosis of PH was 11 years. Eleven of 15 individuals with PAH were on antiretroviral therapy (ART) while all 17 patients with PVH were on ART. Compared to PLWH without PH, those with PH had an increased likelihood of having a detectable HIV viral load and lower CD4 cell counts. PLWH with PAH or PVH had increased RV afterload with normal RV contractility, and preserved RV-PA coupling. </span></span></span></span></span></span></span></span></span></span></span></p> <p class="Body"><span class="None"><span><span><span><span><span><span><span><span><span><span>Conclusion: PLWH with PH (PAH or PVH) were more likely to have a detectable HIV viral load and lower CD4 count at the time of RHC. PLWH with PAH or PVH had increased RV afterload, normal RV contractility, with preserved RV-PA coupling suggestive of an early onset, mild, and compensated form of PH. These results should be confirmed in larger studies.</span></span></span></span></span></span></span></span></span></span></span></p>
Printability, durability, contractility and vascular network formation in 3D bioprinted cardiac endothelial cells using alginate-gelatin hydrogels
<p>TBC</p>
Microscopy imaging of the contractility assay for 2 donors in the different conditions and timepoints (9)
<p>Microscopy imaging of the contractility assay for donor 1, 18h, hpl</p>
Microscopy imaging of the contractility assay for 2 donors in the different conditions and timepoints (3)
<p>Microscopy imaging of the contractility assay for donor 1, 1h inib</p>
Microscopy imaging of the contractility assay for 2 donors in the different conditions and timepoints (7)
<p>Microscopy imaging of the contractility assay for donor 1, 18h, dmem</p>
Microscopy imaging of the contractility assay for 2 donors in the different conditions and timepoints (4)
<p>Microscopy imaging of the contractility assay for donor 1, 2h, DMEM</p>
Microscopy imaging of the contractility assay for 2 donors in the different conditions and timepoints (2)
<p>Microscopy imaging of the contractility assay for donor 1, 1h hpl</p>
Microscopy imaging of the contractility assay for 2 donors in the different conditions and timepoints (5)
<p>Microscopy imaging of the contractility assay for donor 1, 2h hpl</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.