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8 results for “cross-contamination”
Single-cell ATAC-seq control of cross-contaminations (experiment 2)
<p>On the Fluidigm C1 platform for single-cell analysis, the cells are captured in 96 chambers arranged serially, and then washed before further processing. Thus, debris present from the loading medium or released by captured cells upstream are a possible source of contamination. We generated a control datasets using the single-cell ATAC-seq protocol available from Fluidigm's ScriptHub. We cultivated human Hep G2 and mouse Hepa 1-6 (both are liver cancer cell lines), stained them with green and red calceins (respectively), and loaded them at equal concentration in a Fluidigm medium flow cell (old design), before running the C1 single-cell ATAC-seq program. To evaluate damage and carry-over of debris from FACS-sorting, two IFCs were run in two C1 machines in parallel. In the first (flowcell ID 1772-123-155), the cells not washed and in the second, they were washed (ID 1772-123-158).</p> <p>The data deposited here is a sequencing run (Illumina MiSeq) of these ATAC-seq libraries. The metadata indicating the contents of each well is being uploaded separately and this record will be updated once the DOIs are available.</p>
Single-cell ATAC-seq control of cross-contaminations (experiment 1)
<p>On the Fluidigm C1 platform for single-cell analysis, the cells are captured in 96 chambers arranged serially, and then washed before further processing. Thus, debris present from the loading medium or released by captured cells upstream are a possible source of contamination. We generated a control datasets using the single-cell ATAC-seq protocol available from Fluidigm's ScriptHub. We cultivated human Hep G2 and mouse Hepa 1-6 (both are liver cancer cell lines), stained them with green and red calceins (respectively), and loaded them at equal concentration in a Fluidigm medium flow cell (old design), before running the C1 single-cell ATAC-seq program.</p> <p>The data deposited here is a sequencing run (Illumina MiSeq) of these ATAC-seq libraries. The metadata indicating the contents of each well is being uploaded separately and this record will be updated once the DOIs are available.</p>
Figure 1 in A fossil protein chimera; difficulties in discriminating dinosaur peptide sequences from modern cross-contamination
Figure 1. Tandem mass spectrum from high-resolution (HCD) fragmentation analysis of the peptide sequence (GPPGESGAVGPAGPIGSR) matched from our analysis of ostrich bone collagen that is homologous to the peptide proposed as unique to T. rex and B. canadensis.
Figure 2 in A fossil protein chimera; difficulties in discriminating dinosaur peptide sequences from modern cross-contamination
Figure 2. Tandem mass spectrum of the peptide sequence claimed as being endogenous to both dinosaurs with sequence (GLPGESGAVGPAGPPGSR) downloaded from the B. canadensis analysis by Schweitzer et al. [14].
Quantifying and reducing cross-contamination in single- and multiplex hybridization capture of ancient DNA
<p>The use of hybridization capture has enabled a massive upscaling in sample sizes for ancient DNA studies, allowing the analysis of hundreds of skeletal remains (Mathieson et al., 2015; Narasimhan et al., 2019) or sediments (Vernot et al., 2021; Wang et al., 2021; Zavala et al., 2021) in single studies. Yet demands in throughput continue to grow, and hybridization capture has become a limiting step in sample preparation due to the large consumption of reagents, consumables and time. Here we explore the possibility of improving the economics of sample preparation via multiplex capture, i.e. the hybridization capture of pools of double-indexed ancient DNA libraries. We demonstrate that this strategy is feasible for small genomic targets, such as mitochondrial DNA, if the annealing temperature is increased and PCR cycles are limited in post-capture amplification to avoid index swapping by jumping PCR, which manifests as cross-contamination in resulting sequence data. We also show that the re-amplification of double-indexed libraries to PCR plateau before or after hybridization capture can sporadically lead to small, but detectable cross-contamination even if libraries are amplified in separate reactions. We provide protocols for both manual capture and automated capture in 384-well format that are compatible with single- and multiplex capture and effectively suppress cross-contamination and artefact formation. Last, we provide a simple computational method for quantifying cross-contamination due to index swapping in double-indexed libraries, which we recommend using for routine quality checks in studies that are sensitive to cross-contamination. </p>
Cross-contamination effect on turbulence spectra from Doppler beam swinging wind lidar (data and code)
<p>The archive contains supplemental material for the article "Cross-contamination effect on turbulence spectra from Doppler beam swinging wind lidar" by Kelberlau and Mann:</p> <p>- Windcube RAW and 10-min averaged data</p> <p>- Ultrasonic anemometer data from the meteorological mast in Høvsøre</p> <p>- Monin-Obukhov length data</p> <p>- windsimu input files, a windsimu executable for unix systems to create turbulence boxes and a windsimu manual</p> <p>- Matlab scripts for data processing and visualization</p>
Quantifying and reducing cross-contamination in single- and multiplex hybridization capture of ancient DNA
Open the record for dataset details and reuse information.
Pilot Safety Study to Determine the Ability of the Protector Cap Jet Injector to Prevent Cross-Contamination
ClinicalTrials.gov study NCT00219453. IPD Sharing: Not stated. Countries: 0. Publications: 0.
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