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17 results for “cryoprotectant”
FIGURE 5 in Seminal characteristics and sensitivity of Astyanax lacustris (Characiformes: Characidae) sperm to cryoprotective solutions based on dimethylsufoxide and methylglicol
FIGURE 5 | Compressed cell nucleus found in T4 of cryopreservation process of Astyanax lacustris semen.
FIGURE 1 in Seminal characteristics and sensitivity of Astyanax lacustris (Characiformes: Characidae) sperm to cryoprotective solutions based on dimethylsufoxide and methylglicol
FIGURE 1 | Fluorescence photomicrograph of Astyanax lacustris (100X magnification) of nuclei with different types of damage assessed by the Comet Assay: damage 0 - no apparent damage; damage 1 - little damage; damage 2 - average damage; damage 3 - major damage.
Data from: Freeze-tolerant frogs accumulate cryoprotectants using photoperiod: A potential ecological trap
Open the record for dataset details and reuse information.
Fig 1 in Toxicity of cryoprotectants on Prochilodus lineatus (Valenciennes, 1837) (curimba) embryos in an experimental incubator (Characiformes: Prochilodontidae)
Fig 1. Representative image of the experimental incubators.
Reviving diversity: cryoprotectants and culturing methods enhance recovery of mammalian gut microbes from field samples
<p>Welcome!</p> <p>Here you will find the codes used in the analysis we ran for our manuscript titled "Reviving diversity: cryoprotectants and culturing methods enhance recovery of mammalian gut microbes from field samples". We would be happy to help in way we can, so please do not hesitate to reach out if you have questions or suggestions.</p> <p>In summary, we developed this study in response to challenges encountered in our own field research. Recent advancements in culturomics and microbiology are enabling us to further explore the realm of microbiome science. We aspire for this study to serve as a foundational step toward identifying preservation methods that can help protect the microbial communities of wild animals.</p> <p>Our study aims are:</p> <ol> <li>The primary objective of this study was to evaluate the effectiveness of various preservation solutions in maintaining microbial integrity of gut samples during collection and for a short duration, simulating transportation conditions, prior to long-term storage at -80°C. We cultivated the samples in three distinct culture media to maximize the recovery of microbial diversity.</li> <li>The second objective was to compare the inventory of the preserved and cultured microbial community to that of the original uncultured samples, aiming to determine whether the preservation solutions retained unique taxa absent in the frozen original samples.</li> </ol> <p>Notes to keep in mind:</p> <ol> <li>We chose preservation solutions that have already been described for microbial culturing.</li> <li>We chose culture media that have already been described for microbial culturing, especially gut microbiome samples.</li> </ol> <p><em>As you go through the Rmd document included here, please make sure all documents have been properly downloaded. Additionally, please make sure to change the names of the alpha diversity metric files in the Rmd to that of the files here as they were modified for proper uploading methods here. </em></p>
Data from: Direct evidence that cryoprotectant mixtures facilitate individual component permeation into living plant cells
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Cryoprotection of Chemotherapy-induced Oral Mucositis After Autologous Stem Cell Transplantation, a Randomized Study
ClinicalTrials.gov study NCT03203733. IPD Sharing: NO. Countries: 2. Publications: 9.
Effectiveness of Probiotics Stabilized With Cryoprotection Technology in Patients With Irritable Bowel Syndrome
ClinicalTrials.gov study NCT04206410. IPD Sharing: NO. Countries: 1. Publications: 1.
FIGURE 4 in Seminal characteristics and sensitivity of Astyanax lacustris (Characiformes: Characidae) sperm to cryoprotective solutions based on dimethylsufoxide and methylglicol
FIGURE 4 | Index of DNA damage generated by the cryopreservation process of Astyanax lacustris semen. Control - fresh semen, T1 - 10% egg yolk + 5% glucose + Me2SO10%, T2 - 10% egg yolk + 5% glucose + Me 2 SO15%, T3 - 10% egg yolk + 5% glucose + MTG010%, T5 - BTS5% + Me2 SO10%, T6 - BTS5% + Me2 SO15%, T7 - 5% Glucose + Me2 SO10% and T8 - 5% Glucose + Me2SO15%. Different letters indicate significant differences (p <0.05) by the Kruskal-Wallis test.
FIGURE 2 in Seminal characteristics and sensitivity of Astyanax lacustris (Characiformes: Characidae) sperm to cryoprotective solutions based on dimethylsufoxide and methylglicol
FIGURE 2 | Comparative analysis of sperm motility of Astyanax lacustris. Total and progressive motility (A), curvilinear velocity (B), straight line velocity (C) and average path velocity (D). Different letters indicate significant differences (p <0.05) by the Scott-Knott test: Upper case relationship between control and treatments; tiny relationship between treatments.
FIGURE 3 in Seminal characteristics and sensitivity of Astyanax lacustris (Characiformes: Characidae) sperm to cryoprotective solutions based on dimethylsufoxide and methylglicol
FIGURE 3 | Linearity (A), Rectilinearity (B), Oscillation (C), Head lateral displacement amplitude (D) and beat cross frequency (E). Different letters indicate significant differences (p <0.05) by the Scott-Knott test: Upper case relationship between control and treatments; tiny relationship between treatments.
Evaluation of Cryoprotection Induced Nail Toxicity Docetaxel Low Cumulative Dose
ClinicalTrials.gov study NCT02283983. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Comparative transcriptome analysis reveals molecular damage associated with cryopreservation in Crassostrea angulata D-larvae rather than to cryoprotectant exposure
GEO Series GSE246924. Magallana angulata. 9 samples. Type: Expression profiling by high throughput sequencing.
Insights on cryoprotectant toxicity from gene expression profiling of endothelial cells exposed to ethylene glycol
GEO Series GSE67511. Homo sapiens. 18 samples. Type: Expression profiling by array.
A Randomized, Comparative, Double-blind Trial of Pentaisomaltose and Dimethyl Sulphoxide for Cryoprotection of Hematopoietic Stem Cells in Subjects With Multiple Myeloma or Malignant Lymphoma With a N
ClinicalTrials.gov study NCT05545202. IPD Sharing: Not stated. Countries: 0. Publications: 0.
Human gut microbiota preservation for functional studies: in-vitro comparison of common cryoprotective agents
GEO Series GSE77327. uncultured bacterium; human gut metagenome. 15 samples. Type: Other.
Effect of vitrification temperature and cryoprotectant concentrations on the mRNA transcriptome of bovine immature oocytes
GEO Series GSE133550. Bos taurus. 15 samples. Type: Expression profiling by high throughput sequencing.
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