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278 results for “cytometry”
Real-time deformability cytometry reference data
<p>This dataset consists of four exemplary real-time fluorescence and deformability cytometry measurements. The HDF5-files can be opened with dclab [1] or Shape-Out [2].</p> <p><strong>calibration_beads.rtdc</strong><br> The calibartion beads (8 Peaks, PolyAN) consist of eight bead populations with different mixtures of fluorophores.</p> <p><br> <strong>CD34_HSPC.rtdc</strong><br> Hematopoietic stem and progenitor cells (HSPCs) were obtained using apheresis. The cells were tagged with a fluorescently labeled antibody that binds to the CD34 transmembrane protein. CD34-positive HSPCs are gated with `fl3_max > 90`. Set `area_ratio < 1.05` to remove aggregates. Data were used in [3].</p> <p><br> <strong>leukocytes.rtdc</strong><br> The leukocyte population (white blood cells) of this blood sample can be visualized by setting `aspect < 2` and `area_ratio < 1.05`. For more information, see e.g. [4].</p> <p><br> <strong>reticulocytes.rtdc</strong><br> Blood contains mostly red blood cells (RBCs) and about 1% reticulocytes (which develop into mature RBCs). Reticulocytes contain ribosomal RNA which was stained with Syto13 for this measurement. Set `area_ratio < 1.05` to remove aggregates. Data were used in [3].</p> <p><br> [1] <a href="https://github.com/ZellMechanik-Dresden/dclab">https://github.com/ZellMechanik-Dresden/dclab</a></p> <p>[2] <a href="https://github.com/ZellMechanik-Dresden/ShapeOut">https://github.com/ZellMechanik-Dresden/ShapeOut</a></p> <p>[3] Rosendahl et al., "Real-time fluorescence and deformability cytometry". Nature Methods, 15(5):355–358, 2018. doi:<a href="https://dx.doi.org/10.1038/nmeth.4639">10.1038/nmeth.4639</a>.</p> <p>[4] Toepfner et al., "Detection of human disease conditions by single-cell morpho-rheological phenotyping of whole blood". eLife, 7:e29213, 2017. doi:<a href="https://dx.doi.org/10.1101/145078">10.1101/145078</a>.</p> <p><br> SHA256 sums:<br> 08c2ef13eed903ef0f9e451727ab8484df09b5d3b39227dab726e0164dcbe244 calibration_beads.rtdc<br> 663b44a9db88d85996500045489e37a317cf115719223a531d617f8e3d450e79 CD34_HSPC.rtdc<br> 68bd538b42ffb990f1db52d5f3b21f37c9aff31208ab284f3910fd6872c40fdb leukocytes.rtdc<br> 5c323ea75bf7eeb2a28d922730772d50270dd872d6957e60d6062663f3628fb3 reticulocytes.rtdc</p>
Abundance, biovolume, and biomass of Synechococcus and eukaryote pico- and nano- plankton from continuous underway flow cytometry during NES-LTER Transect cruises, ongoing since 2018
These data represent the abundance, biovolume, and biomass of prokaryotic and eukaryotic picoplankton and nanoplankton sampled continuously underway during Northeast U.S. Shelf Long-Term Ecological Research (NES-LTER) Transect cruises, ongoing since 2018. Samples were obtained with an Attune NxT Flow Cytometer sampling at approximately 2-min intervals from the underway science seawater. Cells were identified and enumerated from the flow cytometry data files based on their scattering, phycoerythrin (575 nm) and chlorophyll (680 nm) fluorescence signals.
Abundance, biovolume, and biomass of Synechococcus, eukaryote pico- and nano- phytoplankton, and heterotrophic bacteria from flow cytometry for water column bottle samples on NES-LTER Transect cruises, ongoing since 2018
These data represent the abundance, biovolume, and biomass of prokaryotic phytoplankton, eukaryotic pico- and nano- phytoplankton, and heterotrophic bacteria from discrete flow cytometry samples collected during the Northeast U.S. Shelf Long-Term Ecological Research (NES-LTER) Transect cruises, ongoing since 2018. Samples were collected and preserved from the water column at multiple depths using Niskin bottles on a CTD rosette system along the NES-LTER transect, and analyzed post cruise. Cells were identified and enumerated from the flow cytometry data files based on their scattering, SYBR (525 nm), phycoerythrin (575 nm) and chlorophyll (680 nm) fluorescence signals. Gating was completed manually in the Attune NXT software interface.
Example imaging mass cytometry raw data
<p>If you are working with these files, please cite them as follows:<br><br>Windhager, J., Zanotelli, V.R.T., Schulz, D. et al. An end-to-end workflow for multiplexed image processing and analysis. Nat Protoc (2023). <a href="https://doi.org/10.1038/s41596-023-00881-0">https://doi.org/10.1038/s41596-023-00881-0</a></p><p>This imaging mass cytometry (IMC) dataset serves as an example to demonstrate raw data processing and downstream analysis tools. The data was generated as part of the <strong>I</strong>ntegrated i<strong>MMU</strong>noprofiling of large adaptive <strong>CAN</strong>cer patient cohorts (IMMUcan) project (<a href="https://immucan.eu">immucan.eu</a>) using the Hyperion imaging system (<a href="https://www.fluidigm.com/products-services/instruments/hyperion">www.fluidigm.com/products-services/instruments/hyperion</a>). To get an overview on the technology and available analysis strategies, please visit <a href="https://bodenmillergroup.github.io/IMCWorkflow/">bodenmillergroup.github.io/IMCWorkflow</a>. The individual data files are described below:</p><ul><li><strong>Patient1.zip, Patient2.zip, Patient3.zip, Patient4.zip</strong>: raw data files of 4 patient samples. Each .zip archive contains a folder in which one .mcd file (IMC raw data) and multiple .txt files (one per acquisition) can be found.</li><li><strong>compensation.zip</strong>: This .zip archive holds a folder which contains one .mcd file and multiple .txt files. Multiple spots of a "spillover slide" were acquired and each .txt file is named based on the spotted metal. This data is used for channel spillover correction. For more information, please refer to the original publication: <a href="https://doi.org/10.1016/j.cels.2018.02.010">Compensation of Signal Spillover in Suspension and Imaging Mass Cytometry</a></li><li><strong>panel.csv</strong>: This file contains metadata for each antibody/channel used in the experiment. The <i>full</i> column indicates which channel should be analysed. The <i>ilastik</i> column specifies which channels were used for ilastik pixel classification and the <i>deepcell</i> column indicates the channels used for deepcell segmentation.</li><li><strong>sample_metadata.csv</strong>: This file links each patient to their cancer type (SCCHN - head and neck cancer; BCC - breast cancer; NSCLC - lung cancer; CRC - colorectal cancer).</li></ul>
Combined unsupervised and semi-automated supervised analysis of flow cytometry data reveals cellular fingerprint associated with newly diagnosed pediatric type 1 diabetes
<p>Type 1 diabetes is a chronic autoimmune disease resulting in an immune-mediated loss of pancreatic β-cells; however, an unbiased and reproducible profiling of type 1 diabetes-specific circulating immunome at disease onset has yet to be explored. In this study, fresh whole blood was collected from a pediatric cohort of 107 patients with new-onset type 1 diabetes, 85 relatives of patients with type 1 diabetes with 0-1 islet autoantibodies, 58 patients with celiac disease or autoimmune thyroiditis and 76 healthy controls. Up to 6 mL of blood was collected from each subject into a VACUETTE® TUBE 6 ml ACD-B (Greiner). Fresh whole blood underwent red blood cell lysis, was washed and stained with specific monoclonal antibodies. Fresh whole blood samples were stained with five panels of antibodies labelled as T cells, T&NK cells, B cells, Tregs and DCs/monos encompassing main subsets of T cells, NK cells, B cells, Tregs, DCs and monocytes detected using 26 surface markers and the intracellular marker forkhead box P3 (FoxP3); for the Treg panel, intracellular staining was performed after fixation and permeabilization. Cells were acquired on a BD FACSCanto-II flow cytometer equipped with FACSDiva software (Becton Dickinson, Franklin Lakes, NJ). </p>
GeoWaVe Cytometry Benchmark Data
<p>Contained within this folder are six benchmark datasets (Levine13, Levine32, Samusik, Sepsis, and PD) used for the evaluation of the GeoWaVe ensemble clustering algorithm, part of the cytocluster (https://github.com/burtonrj/CytoCluster) package.</p> <p>The data are compensated, arc-sine transformed, and debris and dead cells removed. See manuscript for details: https://doi.org/10.1101/2022.06.30.496829</p> <p>Each dataset is available as a CSV file and includes two additional columns: UMAP1 and UMAP2. The UMAP columns contain embeddings generated using UMAP (2 components and n_neighbours=30) and were used for visualisation purposes. The column 'population' contains the original population labels generated using manual gating.</p>
Flow cytometry of mesenteric lymph nodes, small and large intestinal lamina propria, and spinal cord cells from fibre-rich and fiber-free diet-fed gnotobiotic mice at baseline and after experimental autoimmune encephalomyelitis (EAE) induction
<p>We perform profiling of different immune cell populations in the small (SILP) and large intestine lamina propria (CLP), mesenteric lymph nodes (MLN) and spinal cords (SC). We are specifically interested to evaluate the impact of dietary fiber deprivation followed by mucus erosion on the immune cell profiles of T helper cells (Th cells, T cell population) of gnotobiotic mice fed a fiber-rich (FR) or fiber-free (FF) diet. This dataset aims to assess the impact of microbiome and diet on disease course in a mouse model of multiple sclerosis (experimental autoimmune encephalomyelitis, EAE) via T cell populations. Mice are either germ-free or colonized by intragastric gavage with a defined variation of a 14-member synthetic human gut microbiome (doi: 10.1016/j.cell.2016.10.043 and 10.1016/j.xpro.2021.100607): SM01 (Akkermansia muciniphila monocolonisation), SM03 (Bacteroides caccae, Bacteroides thetaiotaomicron, Barnesiella intestinihominis), SM04 (B. caccae, B. thetaiotaomicron, B. intestinihominis, A. muciniphila), SM12 (full community except mucin-specialists B. intestinihominis and A. muciniphila), SM13 (full community except mucin specialist A. muciniphila), or SM14 (full community: Roseburia intestinalis, Faecalibacterium prausnitzii, Marvinbryantia formatexigens, Collinsella aerofaciens, Desulfovibrio piger, B. caccae, B. thetaiotaomicron, Bacteroides ovatus, Bacteroides uniformis, B. intestinihominis, Eubacterium rectale, Clostridium symbiosum, Escherichia coli, and A. muciniphila). At age 5 to 8 weeks, mice were colonized with SM combinations while fed an FR diet. Mice were either maintained on an FR diet or switched to an FF diet at 5 days after initial colonization, until the end of experiment. Baseline samples were collected 20 days following the diet switch. Otherwise, EAE induction was performed 15 days after the diet switch and samples were collected 30 days after the induction.</p>
Ultrasensitive detection of cancer-associated nucleic acids and mutations by primer exchange reaction-based signal amplification and flow cytometry
<p>This dataset contains the raw data that were used for the publication entitled, "Ultrasensitive detection of cancer-associated nucleic acids and mutations by primer exchange reaction-based signal amplificaiton and flow cytometry" published in Biosensors and Bioelectronics on 5 October 2024.</p>
Dataset and Code for Manuscript "Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification"
<p>Dataset of measurements for cell cycle analysis with description:</p> <ul> <li>ReadMe file with explanations on the data set and analysis</li> <li>exemplary Matlab script file for analysis</li> <li>binary data files conatining the pulse shapes in all channels</li> <li>FCS data files containing common flow cytometry parameters in each channel</li> </ul> <p>Data on unsorted HEK cells, HEK cells sorted for cell cycle phases, and unsorted Jurkat cell are included.</p>
Picophytoplankton and bacteria abundances analyzed with flow cytometry (FCM) from CCE-CalCOFI Augmented cruises in the California Current System, 2004 - 2023 (ongoing).
Picophytoplankton populations and non-pigmented prokaryotes are sampled within the California Current Ecosystem (CCE) for abundances from 3 to 8 depths at CalCOFI stations. Seawater is collected from Niskin bottles and cells are fixed in the field aboard the survey cruises (since 2004, ongoing) with paraformaldehyde, and stained with a DNA-specific dye back in the laboratory. The cells are enumerated by an Altra flow cytometer (with a syringe pump for volumetric sample delivery) simultaniously with argon ion lasers, to distinguish three major populations of photoautotrophs (Prochlorococcus, Synechococcus, and pico-eukaryotes) and the assemblage of heterotrophic prokaryotes collectively referred to as H-Bact.
Picophytoplankton and bacteria total carbon estimates from cell counts analyzed with flow cytometry (FCM) from CCE-CalCOFI Augmented cruises in the California Current System, 2004 - 2023(ongoing).
Picophytoplankton populations and non-pigmented prokaryotes are sampled within the California Current Ecosystem (CCE) for abundances from 3 to 8 depths at CalCOFI stations. Seawater is collected from Niskin bottles and cells are fixed in the field aboard the survey cruises (since 2004, ongoing) with paraformaldehyde, and stained with a DNA-specific dye back in the laboratory. The cells are enumerated by an Altra flow cytometer (with a syringe pump for volumetric sample delivery) simultaniously with argon ion lasers, to distinguish three major populations of photoautotrophs (Prochlorococcus, Synechococcus, and pico-eukaryotes) and the assemblage of heterotrophic prokaryotes collectively referred to as H-Bact. FCM abundance estimates for each are converted to carbon biomass equivalents using mixed-layer estimates.
Discrete flow cytometry of underway samples from the Gradients 2019 cruise using a BD Influx Cell Sorter
<p>The dataset consists of BD Influx-based analysis of phytoplankton populations from discrete flow cytometry data collected underway during the Gradients 2019 (Gradients 3/KM1906) oceanographic research cruise in the Northeast Pacific Ocean. The analysis includes cell abundance, forward light scatter, and pigment fluorescence of individual cells, including picoeukaryotes and the cyanobacteria Prochlorococcus and Synechococcus. Time is in UTC format, latitude and longitude are in decimal degrees, and depth is in meters. Further information can be found here: https://github.com/fribalet/FCSplankton</p>
Discrete flow cytometry from the Gradients 2019 cruise using a BD Influx Cell Sorter
<p>The dataset consists of BD Influx-based analysis of phytoplankton populations and heterotrophic bacteria from discrete flow cytometry data collected during the Gradients 2019 (Gradients 3/KM1906) oceanographic research cruise in the Northeast Pacific Ocean. The analysis includes cell abundance, forward light scatter, and pigment fluorescence of individual cells, including bacteria, picoeukaryotes, and the cyanobacteria Prochlorococcus and Synechococcus. Time is in UTC format, latitude and longitude are in decimal degrees, and depth is in meters. Further information can be found here: https://github.com/fribalet/FCSplankton</p>
Flow cytometry data from human iPSC-derived macrophages
<p>Human induced pluripotent cells (iPSCs) were obtained from the HipSci project (http://www.hipsci.org) and differentiated into macrophages using an established protocol (van Wilgenburg, 2013). The genotype_id column of the flow_sample_metadata.txt file contains the canonical HipSci iPSC line name from which the macrophages were differentiated.</p> <p><strong>Data acquisition</strong></p> <p>We used flow cytometry to measure the cell surface expression of three canonical macrophage markers: CD14, CD16 (FCGR3A/FCGR3B) and CD206 (MRC1). Macrophages were cultured in 10 cm tissue-culture treated plates and detached from the plates by incubation in 6 mg/ml lidocaine-PBS solution (Sigma L5647) for 30 minutes followed by gentle scraping. From each cell line we harvested between 300,000-500,000 cells. Detached cells were washed in media, centrifuged at 1200 rpm for 5 minutes and resuspended in flow cytometry buffer (2% BSA, 0.001% EDTA in D-PBS) and split into two wells of a 96-well plate. Nonspecific antibody binding sites were blocked by incubating cells with Human TruStain FcX (Biolegend) for 45 minutes and washing with flow cytometry buffer. Half of the cells were stained for 1 hour with the PE-isotype control (BD 555749) antibody. The other half of the cells were co-stained for 1 hour with following three antibodies: CD14-Pacific Blue (BD 558121), CD16-PE (BD 555407), CD206-APC (BD 550889). After staining, the cells were washed three times. Resuspended cells were filtered through cell-strainer cap tubes (BD 352235) and measured on the BD LSRFortessa Cell Analyzer.</p>
OMAP-8: Multiplexed Antibody-Based Imaging of Placenta with Imaging Mass Cytometry (IMC), v1.0
<p>OMAP-8 was designed for Imaging Mass Cytometry (IMC) (<a href="https://pubmed.ncbi.nlm.nih.gov/24584193/">https://pubmed.ncbi.nlm.nih.gov/24584193/</a>) of formalin-fixed paraffin-embedded (FFPE) human term-placenta samples. The tissue slides were prepared with a two-step antigen retrieval process (pH 6 and pH 9, as described <a href="https://dx.doi.org/10.17504/protocols.io.bpwumpew">https://dx.doi.org/10.17504/protocols.io.bpwumpew</a>). OMAP antibodies validated by immunohistochemistry and IMC were conjugated to polymers containing metal isotopes. Conjugated antibodies were used to stain processed human term-placenta tissue simultaneously. Regions of the processed tissue were then acquired on the imaging mass cytometer (Hyperion; Standard BioTools) by laser ablation and visualized. The panel contains 26 antibodies conjugated to unique metal isotopes and iridium marks the DNA. This OMAP provides a spatial context for key placenta cell types in the <a href="https://doi.org/10.48539/HBM446.WGLG.755">ASCT+B v.1.0 table</a>. Single-cell RNA sequencing data were used to guide marker selection for multiplexed tissue imaging. For example, ASCL2, HLA-G, PD-L1, CD68 and LYVE1 allow functionally specialized cell types to be visualized and quantified in the placenta. Note that one of our core antibodies is to LYVE1 but, unlike in other tissues where it is used to mark lymphatic vasculature, here we use it to mark the macrophage of the placenta (Hofbauer cells) – there should be no lymphatics in the placenta.</p>
Single cell analysis by Quantitative image-based cytometry (QIBC)
<p>Quantitative image-based cytometry (QIBC): Employing automated multichannel wild-field microscopy using the Olympus ScanR screening system. This system includes an inverted motorized Olympus IX83 microscope, a motorized stage, IR-laser hardware autofocus, a fast emission filter wheel with single band emission filters. </p> <p>Images were analyzed and processed using ScanR analysis software and TIBCOSpotfire software was used to plot total nuclear pixel intensities and mean (total pixel intensities divided by nuclear area) nuclear intensities.</p>
Discrete Flow Cytometry of Underway Samples from TN427 Using a BD Influx Cell Sorter
<p>The dataset consists of BD Influx-based analysis of phytoplankton populations from discrete flow cytometry data collected underway during the University of Washington School of Oceanography 2024 undergraduate senior thesis research cruise (TN427) from American Samoa. The data consists of cell abundance, cell size (equivalent spherical diameter), carbon quota, and carbon biomass for picophytoplankton populations, namely the cyanobacteria Prochlorococcus and Synechococcus, and small eukaryotic phytoplankton (<5 μm ESD). Time is in UTC format, latitude and longitude are in decimal degrees, and depth is in meters. Further information can be found here: [https://github.com/fribalet/FCSplankton](https://github.com/fribalet/FCSplankton)</p>
SeaFlow data v1: High-resolution abundance, size and biomass of small phytoplankton measured by flow-cytometry
<p>SeaFlow is an underway flow cytometer designed to continuously monitor the optical properties of the smallest phytoplankton from a ship's flow-through seawater system. It collects high-resolution data, generating the equivalent of 1 sample every 3 minutes or every 1 km (for a ship moving at 10 knots).</p> <p>The dataset provides measurements of cell abundance, cell size (equivalent spherical diameter) and carbon biomass for small phytoplankton populations: the cyanobacteria Prochlorococcus, Synechococcus, Crocosphaera, and small eukaryotic phytoplankton (<5 μm ESD). Data processing followed the methods outlined in <a href="https://doi.org/10.1038/s41597-019-0292-2">Ribalet et al. (2019)</a>. For more information, visit the <a href="https://seaflow.netlify.app/">SeaFlow website</a>.</p> <p><strong>New in version 1.6 </strong>The updated dataset includes flow cytometric measurements from 89 cruises, spanning nearly 14,000 hours of observations across 130,000 km of the surface oceans.</p>
Imaging Mass Cytometry Dataset of exhausted and non-exhausted breast cancer microenvironments
<p>A cohort of human breast tumor samples were annotated as having an "exhausted" or "non-exhausted" immune environment based on CyTOF characterization of T cell phenotypes (see Wagner et al. 2019). 12 samples (6 exhausted, 6 non-exhausted) were then selected for further analysis by Imaging Mass Cytometry (IMC) with the goal to compare the two immune environment types and to comprehensively characterize exhaustion-associated spatial features of the tumor microenvironment. For IMC, two consecutive FFPE sections of each sample were stained with two different antibody panels (Protein Panel and RNAscope Panel), and 4-10 regions of interest (ROIs, 1mm x 1mm) were measured on each section. ROIs on consecutive sections were registered manually to be as spatially close as possible.</p>
Tumor-Immune Microenvironment Revealed by Imaging Mass Cytometry in a Metastatic Sarcomatoid Urothelial Carcinoma with a Prolonged Response to Pembrolizumab - IMC data
<blockquote> <p>Sarcomatoid urothelial carcinoma (SUC) is a rare subtype of urothelial carcinoma (UC), that typically presents at an advanced stage compared to more common variants of UC. Locally advanced and metastatic UC have a poor long-term survival following progression on first-line platinum-based chemotherapy. Antibodies directed against the programmed cell death 1 protein (PD-1) or its ligand (PD-L1) are now approved to be used in these scenarios. The need for reliable biomarkers for treatment stratification is still under research. Here we present a novel case report of the first Image Mass Cytometry (IMC) analysis done in SUC to investigate the immune cell repertoire and PD-L1 expression in a patient who presented with metastatic SUC and experienced a prolonged response to the anti-PD1 immune checkpoint inhibitor pembrolizumab after progression on first line chemotherapy. This case report provides an important platform for translating these findings to a larger cohort of UC and UC variants.</p> </blockquote> <p>We make available TIFF files containing imaging mass cytometry data for 4 regions of interest of a sample of metastatic sarcomatoid urothelial carcinoma. The order of the axis in the image stacks is "CYX". The CSV files indicate the identity of the channels.</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.