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14 results for “cytoplasmic male sterility”
Growth and fitness measures of cytoplasmic male sterility (CMS) of Physa acuta
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The genetic basis of cytoplasmic male sterility and fertility restoration in wheat
<p><span><span><span><span><span><span><span><span><span><span><span>Hybrid wheat varieties give higher yields than conventional lines but are difficult to produce due to a lack of effective control of male fertility in breeding lines. One promising system involves the <i>Rf1</i> and <i>Rf3</i> genes that restore fertility of wheat plants carrying <i>Triticum timopheevii</i>-type cytoplasmic male sterility (T-CMS). By genetic mapping and comparative sequence analyses we identified <i>Rf1</i> and <i>Rf3</i> candidates that could restore normal pollen production in transgenic wheat plants carrying T-CMS. We show that Rf1 and Rf3 bind to the mitochondrial <i>orf279</i> transcript and induce cleavage, preventing expression of the CMS trait. The identification of restorer genes in wheat is an important step towards the development of hybrid wheat varieties based on a CMS-<i>Rf</i> system. The characterisation of their mode of action brings new insights into the molecular basis of CMS and fertility restoration in plants.</span></span></span></span></span></span></span></span></span></span></span><br> <br> This dataset includes transcript count and coverage data from 2 RNA-seq experiments looking at gene expression in various male-sterile or male-fertile wheat lines examined in the course of this research.</p>
Cytoplasmic male sterility and abortive seed traits generated through mitochondrial genome editing coupled with allotopic expression of atp1 in tobacco
<p>Genome editing tools have recently been developed that are capable of generating deletions and/or rearrangements in plant mitochondrial genomes. We utilized custom-designed meganuclease constructs targeting the essential mitochondrial gene <em>atp1</em> in tobacco plants carrying a version of atp1 that had been repurposed to function as a nuclear gene. Leaf extracts enriched in mitochondria from three independent genome editing events were evaluated by PacBio sequencing and analysis. Contigs that display sequence homology to the reference mitochondrial genome BA000042 are deposited here. Some of the contigs correspond to the tobacco mitochondrial genome as expected, while others corresponded to sequences from the nucleus where the ancient transfer of portions of the mitochondrial genome became incorporated into the nuclear genome. Still other contigs correspond to the chloroplast genome, or pieces of the chloroplast genome anciently transferred to the nucleus, as small fragments of the chloroplast genome are interspersed in plant mitochondrial genomes. Analysis of contigs of genuine mitochondrial origin demonstrated that the double-strand breaks imposed by the genome editing enzymes resulted in the deletion of <em>atp1</em> and neighboring sequences, along with rearrangements in overall genome structure.</p>
The genetic basis of cytoplasmic male sterility and fertility restoration in wheat
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Cytoplasmic male sterility and abortive seed traits generated through mitochondrial genome editing coupled with allotopic expression of atp1 in tobacco
Open the record for dataset details and reuse information.
Data from: Genome-wide association study of outcrossing in cytoplasmic male sterile lines of rice
Stigma exsertion and panicle enclosure of male sterile lines are two key determinants of outcrossing in hybrid rice seed production. Based on 43,394 single nucleotide polymorphism markers, 217 cytoplasmic male sterile lines were assigned into two subpopulations and a mixed-group where the LD decay distance varied from 975 to 2,690 kb. Genome-wide association studies (GWAS) were performed for stigma exsertion rate (SE), panicle enclosure rate (PE) and seed-setting rate (SSR). A total of 154 significant association signals (P < 0.001) were identified. They were situated in 27 quantitative trait loci (QTLs), including 11 for SE, 6 for PE, and 10 for SSR. It was shown that six of the ten QTLs for SSR were tightly linked to QTLs for SE or/and PE with the expected allelic direction. These QTL clusters could be targeted to improve the outcrossing of female parents in hybrid rice breeding. Our study also indicates that GWAS-base QTL mapping can complement and enhance previous QTL information for understanding the genetic relationship between outcrossing and its related traits.
Data from: Genome-wide association study of outcrossing in cytoplasmic male sterile lines of rice
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Data from: Transcript levels of orf288 are associated with the hau cytoplasmic male sterility system and altered nuclear gene expression in Brassica juncea
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Identification of differentially expressed genes related to cytoplasmic male sterility in radish (Raphanus sativus L.) by comparative transcriptome analysis
GEO Series GSE65142. Raphanus sativus. 8 samples. Type: Expression profiling by high throughput sequencing.
Transcriptome Sequencing and De Novo Analysis of a Cytoplasmic Male Sterile Line and Its Near-Isogenic Restorer Line in Chili Pepper (Capsicum annuum L.)
GEO Series GSE45431. Capsicum annuum. 2 samples. Type: Expression profiling by high throughput sequencing.
Global gene expression profiles among five independent cytoplasmic male sterile lines.
GEO Series GSE18057. Oryza sativa. 36 samples. Type: Expression profiling by array.
High-throughput sequencing and degradome analysis reveal differential expression of miRNAs and their targets between cytoplasmic male-sterile cybrid pummelo and its fertile type (Citrus grandis)
GEO Series GSE76668. Citrus maxima. 13 samples. Type: Non-coding RNA profiling by high throughput sequencing; Other.
miRNA profiling in cytoplasmic male sterile (CMS) and its maintainer fertile (MF) lines of Brassica juncea by deep sequencing
GEO Series GSE43244. Brassica juncea. 4 samples. Type: Non-coding RNA profiling by high throughput sequencing; Other.
Mitochondrial Genome Comparison and Functional Analysis of atp8 Underlying Cytoplasmic Male Sterility in Medicago sativa L.
GEO Series GSE317300. Medicago sativa. 9 samples. Type: Expression profiling by high throughput sequencing.
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