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12 results for “dsRNA viruses”
Can immune gene silencing via dsRNA feeding promote pathogenic viruses to control the globally invasive Argentine ant?
<p><span>Pest control methods that can target pest species with limited environmental impacts are a conservation and economic priority. Species-specific pest control using RNA interference is a challenging but promising avenue in developing the next generation of pest management. We investigate the feasibility of manipulating a biological invader's immune system using double-stranded RNA (dsRNA) in order to increase susceptibility to naturally occurring pathogens. We used the invasive Argentine ant as a model, targeting the immunity-associated genes <em>Spaetzle</em> and <em>Dicer-1</em> with dsRNA. We show that feeding of <em>Spaetzle</em> dsRNA can result in partial target gene silencing for up to 28 days in the laboratory and five days in the field. <em>Dicer-1</em> dsRNA only resulted in partial gene knockdown after two days in the laboratory. Double-stranded RNA treatments were associated with significant gene expression disruptions across immune pathways in the laboratory and to a lower extent in the field. We observed occasional changes in viral loads in dsRNA-treated groups. However, immune pathways disruption did not result in consistent increase in microbial infections, nor did they alter ant abundance in the field. Our study explores the feasibility of lowering a pest's immunity as a control tool. We demonstate that it is possible to alter immune gene expression of pest species and pathogen loads, though in our system the affected pathogens did not appear to influence pest abundance. We provide advice on future directions for dsRNA-mediated immune disruption in pest species, including potential avenues to improve dsRNA delivery as well as the importance of the biology of the pest system and its pathogens.</span></p>
Can immune gene silencing via dsRNA feeding promote pathogenic viruses to control the globally invasive Argentine ant?
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Principles of RNA recruitment to viral ribonucleoprotein condensates in a segmented dsRNA virus
<p><strong>Rotaviruses transcribe eleven distinct protein-coding RNAs that must be stoichiometrically co-packaged prior to their replication to make an infectious virion. During infection, </strong><strong>rotavirus transcripts accumulate in cytoplasmic ribonucleoprotein (RNP) condensates, termed viroplasms. </strong><strong>Understanding the mechanisms of viroplasm assembly and RNA enrichment within is crucial to gaining greater insight into their function and stoichiometric assortment of individual transcripts.</strong> <strong>We analysed the subcellular distribution of individual RV transcripts and viroplasm transcriptome by combining multiplexed DNA-barcoded single-molecule RNA FISH of infected cells. Using DNA-PAINT microscopy, we provide evidence of the early onset of viral transcript oligomerisation that occurs prior to the formation of viroplasms. We demonstrate that viral sequences lacking the conserved terminal regions fail to undergo enrichment in rotavirus RNP condensates. We show that individual viral transcripts exhibit variable propensities to partition into viroplasms, irrespective of their absolute numbers in cells, suggesting a selective RNA enrichment mechanism distinct from other known cellular RNP granules. </strong><strong>We suggest that rotavirus replication factories represent unique RNP condensates enriched in eleven types of cognate transcripts that may facilitate the assembly of a multi-segmented RNA genome.</strong></p>
Infection by dsRNA viruses is associated with enhanced sporulation efficiency in Saccharoymces cerevisiae
<p>Upon starvation, diploid cells of the facultative sexual yeast <em>Saccharoymces cerevisiae</em> undergo sporulation, forming four metabolically quiescent and robust haploid spores encased in a degradable ascus. All endosymbionts, whether they provide net benefits or costs, utilise host resources; in yeast, this should induce an earlier onset of sporulation. Here, we tested whether the presence of endosymbiotic dsRNA viruses (M satellite and L-A helper) correspond with higher sporulation rate of their host, <em>S. cerevisiae</em>. We find that <em>S. cerevisiae</em> hosting both the M and L-A viruses (so-called "killer yeasts") have significantly higher sporulation efficiency than those without. We also found that the removal of the M virus did not reduce sporulation frequency, possibly because the L-A virus still utilises host resources with and without the M virus. Our findings indicate that either virulent resource use by endosymbionts induces sporulation, or that viruses are spread more frequently to sporulating strains. Further exploration is required to distinguish cause from effect.</p>
Infection by dsRNA viruses is associated with enhanced sporulation efficiency in Saccharoymces cerevisiae
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Source data for Incarbone et al (2021) - "Immunocapture of dsRNA-bound proteins provides insight into tobacco rattle virus replication complexes and reveals Arabidopsis DRB2 to be a wide-spectrum antiviral effector"
<p>Source data for Incarbone et al (2021) - "Immunocapture of dsRNA-bound proteins provides insight into tobacco rattle virus replication complexes and reveals Arabidopsis DRB2 to be a wide-spectrum antiviral effector"</p> <p>Includes full scans of blots mounted in figures and additional microscopy acquisitions, including brightfield channel</p>
Nanopore Sequencing of Double-Stranded RNA (dsRNA) for Plant Virus and Viroid Detection
<p>Thi file contain results of 24 grapevines leaf samples analyzed using dsRNA-MiSeq (Illumina Miseq) and dsRNAcD sequencing (ONT nanopore), that were used in the following article ''<strong>Nanopore Sequencing of Double-Stranded RNA (dsRNA) for Plant Virus and Viroid Detection'' </strong> submitted in Frontiers in Microbiology </p>
Next Generation Sequencing Facilitates Quantitative Analysis of yeast cells lacking either M-2 dsRNA or both L-A-lus and M-2 dsRNA viruses
GEO Series GSE100290. Saccharomyces cerevisiae. 9 samples. Type: Expression profiling by high throughput sequencing.
Comparative analysis of RNA interference and pattern-triggered immunity induced by dsRNA in response to virus infection
GEO Series GSE253964. Nicotiana benthamiana. 12 samples. Type: Expression profiling by high throughput sequencing.
Additional dataset for 'Principles of RNA recruitment to viral ribonucleoprotein condensates in a segmented dsRNA virus'
<p>Additional dataset - RNA FISH data set for Figure 6b</p>
Comparative Analysis of Genes Induced by Respiratory Syncytial Virus and DsRNA in Human Epithelial Cells
GEO Series GSE12144. Homo sapiens. 4 samples. Type: Expression profiling by array.
Mosquito hematophage-mediated GABAergic activation facilitates acquisition of mosquito-borne viruses (dsRNA knocked-down)
GEO Series GSE91032. Aedes aegypti. 3 samples. Type: Expression profiling by high throughput sequencing.
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International Brain Laboratory public data
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OpenNeuro
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