Find research datasets worth reusing

Fig. 7 in An unusually elongate endogeic centipede from Sardinia (Chilopoda: Geophilidae)

Fig. 7. Variation in the elongation of legs and the number of legs in a sample of species of Geophilidae, including Endogeophilus ichnusae gen. et sp. nov. The length/width ratio of a leg tarsus was measured at about 20% of the longitudinal series of legs. Full names of species in 'Material and methods'.

Fig. 2 in An unusually elongate endogeic centipede from Sardinia (Chilopoda: Geophilidae)

Fig. 2. Endogeophilus ichnusae gen. et sp. nov., holotype, ♀, forcipular segment, left half. A. Dorsal view. B. Ventral view. Scale bar = 100 µm.

Fig. 1 in An unusually elongate endogeic centipede from Sardinia (Chilopoda: Geophilidae)

Fig. 1. Endogeophilus ichnusae gen. et sp. nov. A–F. Holotype, ♀. A. Anterior part of the body, dorsal view, setae omitted. B. Head, without antennae, and forcipular segment, dorsal view. C. Leg-bearing segment 21, dorsal view. D. Leg-bearing segment 21, ventral view. E. Head, without antennae, ventral view. F. Ultimate leg-bearing segment and postpedal segments, ventral view, setae omitted. G. Paratype B, ³, ultimate leg-bearing segment and postpedal segments, ventral view, setae omitted. Line drawings based on photos taken at the microscope. Scale bars = 200 µm.

Fig. 6 in An unusually elongate endogeic centipede from Sardinia (Chilopoda: Geophilidae)

Fig. 6. Variation in the elongation of a single leg-bearing segment in a sample of species of Geophilidae, including Endogeophilus ichnusae gen. et sp. nov. The length/width ratio was measured on a metasternite at about 20% of the longitudinal series of leg-bearing segments, and the area of the metasternite (length × width, both in µm, log-transformed) has been taken as a proxy for body size. Full names of species in 'Material and methods'.

Fig. 8 in An unusually elongate endogeic centipede from Sardinia (Chilopoda: Geophilidae)

Fig. 8. Variation in the length of setae in a sample of species of Geophilidae, including Endogeophilus ichnusae gen. et sp. nov. The longest seta on the cephalic plate was measured, and the maximum width of the cephalic plate has been taken as a proxy for body size. Full names of species in 'Material and methods'.

opencc-by-4.0Mar 2009View details →

Fig. 15. Elongate metacarpals 4 and 5 in Long-Legged Pursuit Carnivorans (Amphicyonidae, Daphoeninae) From The Early Miocene Of North America

Fig. 15. Elongate metacarpals 4 and 5 of (A) Borocyon neomexicanus (F:AM 68242) from Standing Rock Quarry and (B) B. robustum (F:AM 68254) from Blick Quarry, Sandoval Co., New Mexico, demonstrating that the Borocyon lineage persisted in the southwestern United States from latest Arikareean into the early Hemingfordian. The only Hemingfordian record of Borocyon in the Southwest are these two metacarpals from Blick Quarry.

opencc-by-4.0Oct 2018View details →

FIGURES 11–14. Euglossine taxa not exhibiting elongate, necklike prothoracic segment. 11 in On Egg Eclosion and Larval Development in Euglossine Bees

FIGURES 11–14. Euglossine taxa not exhibiting elongate, necklike prothoracic segment. 11. Eulaema (Apeulaema) nigrita modified from Zucchi et al. (1969a: fig. 8) and 12, 13. Eufriesea surinamensis from Rozen (2016: figs. 6, 7) (both of which were identified as predefecating). 14. Exaerete smaragdina, identified as postdefecating in Garófalo and Rozen (2001: fig. 28), presumably was in an early stage that had not yet started to develop pupal tissue internally.

opencc-by-4.0Nov 2023View details →

Figs 1–2 in Scaphobaeocera setosa sp. nov., an unusual Scaphidiinae (Coleoptera: Staphylinidae) with elongate tarsal setae from China

Figs 1–2. Scaphobaeocera setosa Löbl, sp. nov. 1 – body in lateral view; 2 – protibia and protarsus. Scale bars: 0.5 mm (Fig. 1); 0.25 mm (Fig. 2).

opencc-by-4.0Nov 2023View details →

Figs 3–5 in Scaphobaeocera setosa sp. nov., an unusual Scaphidiinae (Coleoptera: Staphylinidae) with elongate tarsal setae from China

Figs 3–5. Scaphobaeocera setosa Löbl, sp. nov. 3 – aedeagus in dorsal view; 4 – same in lateral view; 5 – paramere in ventral view. Scale bars: 0.1 mm (Figs 3–4); 0.05 mm (Fig. 5).

opencc-by-4.0Dec 2023View details →

Text-fig. 3. Pinaceae, Taxaceae. a: Pinus needle fascicle with 3 needles, UAPC-ALTA S 25088A. b: Pinus needle fascicle with at least 4 needles, UAPC-ALTA S 59496. c: Articulate Pinus seed (section Diploxylon) showing seed body partly detached from wing, BBM-PAL-P000007. d: Winged pinaceous seed with elongate, flattened wing and narrow seed body, BBM-PAL-P000048. e: Another winged pinaceous seed with very narrow seed body, BBM-PAL-P000008. f: Amentotaxus leaf, UAPC-ALTA S S25086A. g: Higher magnification counterpart of (f) showing abaxial (lower) leaf surface with two parallel stomatal bands and tapered leaf tip S 25086B. h: Higher magnification of specimen in (f) showing adaxial (upper) leaf surface with detail of single midvein. Scale bars: a–e, g, h = 1 cm, f = 2 cm. in The Early Eocene Flora Of Horsefly, British Columbia, Canada And Its Phytogeographic Significance

Text-fig. 3. Pinaceae, Taxaceae. a: Pinus needle fascicle with 3 needles, UAPC-ALTA S 25088A. b: Pinus needle fascicle with at least 4 needles, UAPC-ALTA S 59496. c: Articulate Pinus seed (section Diploxylon) showing seed body partly detached from wing, BBM-PAL-P000007. d: Winged pinaceous seed with elongate, flattened wing and narrow seed body, BBM-PAL-P000048. e: Another winged pinaceous seed with very narrow seed body, BBM-PAL-P000008. f: Amentotaxus leaf, UAPC-ALTA S S25086A. g: Higher magnification counterpart of (f) showing abaxial (lower) leaf surface with two parallel stomatal bands and tapered leaf tip S 25086B. h: Higher magnification of specimen in (f) showing adaxial (upper) leaf surface with detail of single midvein. Scale bars: a–e, g, h = 1 cm, f = 2 cm.

opencc-by-4.0Dec 2023View details →

Text-fig. 4. Monocots. a, b: Large monocot leaf part and counterpart, UAPC-ALTA S 17955A, B. a: Wide leaf showing entire margin at left. b: Counterpart showing dark wide midrib, and and secondaries parallel to one another, arising at low acute angle. c–e: Monocot leaf with parallel venation. c: Overview of elongate monocot leaf with parallel veins horizontal and linear to oval structures and smaller leaf fragment of same type lacking them (at lower right), UAPC-ALTA S 59491. d: Higher magnification of the smaller fragment with weak cross veins. e: Higher magnification of larger specimen with linear to oval structures between parallel veins. f, g: Monocot leaf with parallel venation. Fig. (f) shows higher magnification and (g) shows overview, BBM-PAL-P000009. Scale bars: a, b = 5 cm, c = 4 cm, d–f = 1 cm, g = 2 cm. in The Early Eocene Flora Of Horsefly, British Columbia, Canada And Its Phytogeographic Significance

Text-fig. 4. Monocots. a, b: Large monocot leaf part and counterpart, UAPC-ALTA S 17955A, B. a: Wide leaf showing entire margin at left. b: Counterpart showing dark wide midrib, and and secondaries parallel to one another, arising at low acute angle. c–e: Monocot leaf with parallel venation. c: Overview of elongate monocot leaf with parallel veins horizontal and linear to oval structures and smaller leaf fragment of same type lacking them (at lower right), UAPC-ALTA S 59491. d: Higher magnification of the smaller fragment with weak cross veins. e: Higher magnification of larger specimen with linear to oval structures between parallel veins. f, g: Monocot leaf with parallel venation. Fig. (f) shows higher magnification and (g) shows overview, BBM-PAL-P000009. Scale bars: a, b = 5 cm, c = 4 cm, d–f = 1 cm, g = 2 cm.

Ditrect STORM imaging and image reconstruction of the transcription elongation marker P-S2 and nuclear PI(3,4)P2 indirectly immunolabeled with AF647 (red) and AF555 (green) in THZ1 treated cells.

U-2 OS cells were grown in DMEM with 10% FBS at 37°C and 5% CO2. Cells were plated one day before staining in ~50% confluence on the high-precision 12 mm round coverslips treated with Hellmanex, sonicated, washed, dried and sterilized. Cells were treated for 3h with 1 µM THZ1 (MedChem HY80013) added to the culture media. U2OS cells were washed twice with PBS (pH 7.4) and fixed for 30 min in 2% PFA in PBS, washed 3-times for 5 min with PBS, then permeabilized in 0.1% Triton X-100 in PBS for 20 min, washed 3-times for 5 min by PBS and blocked in filtered 5% BSA in PBS for 30 min. Cells were incubated for 45 min with rabbit polyclonal IgG anti-RNAPII CTD P-S2 (Abcam ab5095) 3 µg/mL and mouse monoclonal IgG2 anti-PI(3,4)P2 (Z-P034; Echelon Biosci. Inc., USA) 5 µg/mL  in 5% BSA in PBS, washed 3-times for 5 min in PBS and incubated for 30 min with donkey anti-mouse IgG AF555 (Invitrogen A31570) 10 µg/mL and goat anti-rabbit IgG AF647 (Invitrogen A21245) 10 µg/mL diluted in 5% BSA in PBS. Then the cells were washed 3-times for 5 min in PBS, post-fixed for 15 min in 2% PFA in PBS and washed 3-times for 5 min in PBS. All procedures were performed at RT and the cells were stored in PBS in the fridge overnight prior imaging. Coverslips with cells were mounted in the Chamlide chamber (Live Cell Instrument, Korea) and covered with imaging buffer (PBS pH 7.4, 50 mM MEA). Single-molecule localizations (SMLs) data were acquired by Zeiss Elyra PS.1 equipped with HR Diode 642-150 and HR DPSS 561-200 lasers, Alpha Plan-Apochromat 100x/1.46 oil DIC M27 Elyra objective and Andor EM CCD iXon DU 897 camera and Zeiss ZEN Black 2.1 SP3 software (Zeiss). AF647 and AF555 photo-switching was achieved by HiLo illumination and TIRF HP FOV with 100% power of 642nm or 561nm laser, and the signal was acquired via MBS 642 + EF LP 655 and MBS 561 + EF BP 570-620 / LP 750 filters, respectively. Exposure time was 40 ms and EM gain was 300 for both channels. SMLs were calculated in 2D by Zeiss ZEN Black 2.1 SP3 software using x,y 2D Gauss fit with point spread function (PSF) half width 177.9 nm, peak mask size 9 pixels and peak intensity to noise 6 and accounted for overlap in 2D with max cluster size 10. SMLs were rendered in ZEN software with 10 nm/px resolution and 1x PSF expansion factor. The data were model-based drift corrected in ZEN. Two channels were aligned using tetraspec beads fiducial markers for affine calibration. Drift-corrected and aligned localization coordinates were exported as text files. Text files were converted into csv files and imported using self-written macro (Hoboth et al., 2021a) into the ImageJ2 (Rueden et al., 2017) plug-in ThunderSTORM, visualized by normalized Gaussian method (Ovesny et al., 2014).

Ditrect STORM imaging and image reconstruction of the transcription elongation marker P-S2 and nuclear PI(3,4)P2 indirectly immunolabeled with AF647 (red) and AF555 (green) in control cells.

U-2 OS cells were grown in DMEM with 10% FBS at 37°C and 5% CO2. Cells were plated one day before staining in ~50% confluence on the high-precision 12 mm round coverslips treated with Hellmanex, sonicated, washed, dried and sterilized. Cells were control treated with 1:000 DMSO in the culture media. U2OS cells were washed twice with PBS (pH 7.4) and fixed for 30 min in 2% PFA in PBS, washed 3-times for 5 min with PBS, then permeabilized in 0.1% Triton X-100 in PBS for 20 min, washed 3-times for 5 min by PBS and blocked in filtered 5% BSA in PBS for 30 min. Cells were incubated for 45 min with rabbit polyclonal IgG anti-RNAPII CTD P-S2 (Abcam ab5095) 3 µg/mL and mouse monoclonal IgG2 anti-PI(3,4)P2 (Z-P034; Echelon Biosci. Inc., USA) 5 µg/mL  in 5% BSA in PBS, washed 3-times for 5 min in PBS and incubated for 30 min with donkey anti-mouse IgG AF555 (Invitrogen A31570) 10 µg/mL and goat anti-rabbit IgG AF647 (Invitrogen A21245) 10 µg/mL diluted in 5% BSA in PBS. Then the cells were washed 3-times for 5 min in PBS, post-fixed for 15 min in 2% PFA in PBS and washed 3-times for 5 min in PBS. All procedures were performed at RT and the cells were stored in PBS in the fridge overnight prior imaging. Coverslips with cells were mounted in the Chamlide chamber (Live Cell Instrument, Korea) and covered with imaging buffer (PBS pH 7.4, 50 mM MEA). Single-molecule localizations (SMLs) data were acquired by Zeiss Elyra PS.1 equipped with HR Diode 642-150 and HR DPSS 561-200 lasers, Alpha Plan-Apochromat 100x/1.46 oil DIC M27 Elyra objective and Andor EM CCD iXon DU 897 camera and Zeiss ZEN Black 2.1 SP3 software (Zeiss). AF647 and AF555 photo-switching was achieved by HiLo illumination and TIRF HP FOV with 100% power of 642nm or 561nm laser, and the signal was acquired via MBS 642 + EF LP 655 and MBS 561 + EF BP 570-620 / LP 750 filters, respectively. Exposure time was 40 ms and EM gain was 300 for both channels. SMLs were calculated in 2D by Zeiss ZEN Black 2.1 SP3 software using x,y 2D Gauss fit with point spread function (PSF) half width 177.9 nm, peak mask size 9 pixels and peak intensity to noise 6 and accounted for overlap in 2D with max cluster size 10. SMLs were rendered in ZEN software with 10 nm/px resolution and 1x PSF expansion factor. The data were model-based drift corrected in ZEN. Two channels were aligned using tetraspec beads fiducial markers for affine calibration. Drift-corrected and aligned localization coordinates were exported as text files. Text files were converted into csv files and imported using self-written macro (Hoboth et al., 2021a) into the ImageJ2 (Rueden et al., 2017) plug-in ThunderSTORM, visualized by normalized Gaussian method (Ovesny et al., 2014).

Ditrect STORM imaging and image reconstruction of the transcription elongation marker P-S2 and nuclear PI(4,5)P2 indirectly immunolabeled with AF647 (red) and AF555 (green) in control cells.

U-2 OS cells were grown in DMEM with 10% FBS at 37°C and 5% CO2. Cells were plated one day before staining in ~50% confluence on the high-precision 12 mm round coverslips treated with Hellmanex, sonicated, washed, dried and sterilized. Cells were control treated with 1:000 DMSO in the culture media. U2OS cells were washed twice with PBS (pH 7.4) and fixed for 30 min in 2% PFA in PBS, washed 3-times for 5 min with PBS, then permeabilized in 0.1% Triton X-100 in PBS for 20 min, washed 3-times for 5 min by PBS and blocked in filtered 5% BSA in PBS for 30 min. Cells were incubated for 45 min with rabbit polyclonal IgG anti-RNAPII CTD P-S2 (Abcam ab5095) 3 µg/mL and mouse ascites IgM anti-PI(4,5)P2 2C11 (Z-A045; Echelon Biosci. Inc., USA) 5 µg/mL  in 5% BSA in PBS, washed 3-times for 5 min in PBS and incubated for 30 min with goat anti-mouse IgM (µ-chain) AF555 (Jackson ImmunoRes. A24126) 10 µg/mL; goat anti-rabbit IgG AF647 (Invitrogen A21245) 10 µg/mL diluted in 5% BSA in PBS. Then the cells were washed 3-times for 5 min in PBS, post-fixed for 15 min in 2% PFA in PBS and washed 3-times for 5 min in PBS. All procedures were performed at RT and the cells were stored in PBS in the fridge overnight prior imaging. Coverslips with cells were mounted in the Chamlide chamber (Live Cell Instrument, Korea) and covered with imaging buffer (PBS pH 7.4, 50 mM MEA). Single-molecule localizations (SMLs) data were acquired by Zeiss Elyra PS.1 equipped with HR Diode 642-150 and HR DPSS 561-200 lasers, Alpha Plan-Apochromat 100x/1.46 oil DIC M27 Elyra objective and Andor EM CCD iXon DU 897 camera and Zeiss ZEN Black 2.1 SP3 software (Zeiss). AF647 and AF555 photo-switching was achieved by HiLo illumination and TIRF HP FOV with 100% power of 642nm or 561nm laser, and the signal was acquired via MBS 642 + EF LP 655 and MBS 561 + EF BP 570-620 / LP 750 filters, respectively. Exposure time was 40 ms and EM gain was 300 for both channels. SMLs were calculated in 2D by Zeiss ZEN Black 2.1 SP3 software using x,y 2D Gauss fit with point spread function (PSF) half width 177.9 nm, peak mask size 9 pixels and peak intensity to noise 6 and accounted for overlap in 2D with max cluster size 10. SMLs were rendered in ZEN software with 10 nm/px resolution and 1x PSF expansion factor. The data were model-based drift corrected in ZEN. Two channels were aligned using tetraspec beads fiducial markers for affine calibration. Drift-corrected and aligned localization coordinates were exported as text files. Text files were converted into csv files and imported using self-written macro (Hoboth et al., 2021a) into the ImageJ2 (Rueden et al., 2017) plug-in ThunderSTORM, visualized by normalized Gaussian method (Ovesny et al., 2014).

Ditrect STORM imaging and image reconstruction of the transcription elongation marker P-S2 and nuclear PI(4,5)P2 indirectly immunolabeled with AF647 (red) and AF555 (green) in DRB treated cells.

U-2 OS cells were grown in DMEM with 10% FBS at 37°C and 5% CO2. Cells were plated one day before staining in ~50% confluence on the high-precision 12 mm round coverslips treated with Hellmanex, sonicated, washed, dried and sterilized. Cells were treated for 2h with 100 µM DRB (Sigma D1916) added to the cell culture media. U2OS cells were washed twice with PBS (pH 7.4) and fixed for 30 min in 2% PFA in PBS, washed 3-times for 5 min with PBS, then permeabilized in 0.1% Triton X-100 in PBS for 20 min, washed 3-times for 5 min by PBS and blocked in filtered 5% BSA in PBS for 30 min. Cells were incubated for 45 min with rabbit polyclonal IgG anti-RNAPII CTD P-S2 (Abcam ab5095) 3 µg/mL and mouse ascites IgM anti-PI(4,5)P2 2C11 (Z-A045; Echelon Biosci. Inc., USA) 5 µg/mL  in 5% BSA in PBS, washed 3-times for 5 min in PBS and incubated for 30 min with goat anti-mouse IgM (µ-chain) AF555 (Jackson ImmunoRes. A24126) 10 µg/mL; goat anti-rabbit IgG AF647 (Invitrogen A21245) 10 µg/mL diluted in 5% BSA in PBS. Then the cells were washed 3-times for 5 min in PBS, post-fixed for 15 min in 2% PFA in PBS and washed 3-times for 5 min in PBS. All procedures were performed at RT and the cells were stored in PBS in the fridge overnight prior imaging. Coverslips with cells were mounted in the Chamlide chamber (Live Cell Instrument, Korea) and covered with imaging buffer (PBS pH 7.4, 50 mM MEA). Single-molecule localizations (SMLs) data were acquired by Zeiss Elyra PS.1 equipped with HR Diode 642-150 and HR DPSS 561-200 lasers, Alpha Plan-Apochromat 100x/1.46 oil DIC M27 Elyra objective and Andor EM CCD iXon DU 897 camera and Zeiss ZEN Black 2.1 SP3 software (Zeiss). AF647 and AF555 photo-switching was achieved by HiLo illumination and TIRF HP FOV with 100% power of 642nm or 561nm laser, and the signal was acquired via MBS 642 + EF LP 655 and MBS 561 + EF BP 570-620 / LP 750 filters, respectively. Exposure time was 40 ms and EM gain was 300 for both channels. SMLs were calculated in 2D by Zeiss ZEN Black 2.1 SP3 software using x,y 2D Gauss fit with point spread function (PSF) half width 177.9 nm, peak mask size 9 pixels and peak intensity to noise 6 and accounted for overlap in 2D with max cluster size 10. SMLs were rendered in ZEN software with 10 nm/px resolution and 1x PSF expansion factor. The data were model-based drift corrected in ZEN. Two channels were aligned using tetraspec beads fiducial markers for affine calibration. Drift-corrected and aligned localization coordinates were exported as text files. Text files were converted into csv files and imported using self-written macro (Hoboth et al., 2021a) into the ImageJ2 (Rueden et al., 2017) plug-in ThunderSTORM, visualized by normalized Gaussian method (Ovesny et al., 2014).