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317 results for “embryogenesis”
Figs 3-7 in Body pigmentation during embryogenesis first found in stoneflies: a case of Megaperlodes niger Yokoyama, Isobe & Yamamoto, 1990 (Insecta: Plecoptera, Perlodidae)
Figs 3-7 – Embryonic development and the first instar nymph of Megaperlodes niger. 3–6, Embryonic development, Stage 10 (3), Early Stage 11 (4), Later Stage 11 (5), Stage 12 (6), lateral view, anterior at the top; 7, First instar nymph, habitus, dorsal view, anterior third of the left side of antenna artificially lacking. Abbreviations: Ab2, second abdominal segment; An, antenna; Ce, cercus; CE, compound eye; Co, collar; ET, egg tooth; F, fat body; H, head; Hg, hindgut; Mg, midgut; MxP, maxillary palp; SDO, secondary dorsal organ; Th1–3, first, second, and third thoracic segments. Scale bars: 100 µm (Figs 3–6); 500 µm (Fig. 7).
Fig. 9. a in Structural analysis of the Pimelodus maculatus (Lacépède, 1803) embryogenesis (Siluriformes: Pimelodidae)
Fig. 9. a - head detail, showing the otic vesicle, oral cavity, cephalic vesicle, closed mouth and eye. b - myogenesis process;
Fig. 2 in Structural analysis of the Pimelodus maculatus (Lacépède, 1803) embryogenesis (Siluriformes: Pimelodidae)
Fig. 2. Pimelodus maculatus embryos in segmentation stage: a - neurula; b - embryo with about 11 somites, optic vesicle,
Fig. 1 in Structural analysis of the Pimelodus maculatus (Lacépède, 1803) embryogenesis (Siluriformes: Pimelodidae)
Fig. 1. Embryonic development stages zygote, cleavage and gastrula in the Pimelodus maculatus. a - post-fertilization
Fig. 3 in Structural analysis of the Pimelodus maculatus (Lacépède, 1803) embryogenesis (Siluriformes: Pimelodidae)
Fig. 3. Pimelodus maculatus embryos in organogenesis (late segmentation phase) and hatching stage. a – more than 30
Fig. 6 in Structural analysis of the Pimelodus maculatus (Lacépède, 1803) embryogenesis (Siluriformes: Pimelodidae)
Fig. 6. Histologicals sections of Pimelodus maculatus embryos. a- detail of the nucleus of the syncyctial layer, at morula
Fig. 7. a in Structural analysis of the Pimelodus maculatus (Lacépède, 1803) embryogenesis (Siluriformes: Pimelodidae)
Fig. 7. a - embryo section at gastrula stage (epiboly of 75%), staining: HE; b - embryo section at gastrula stage (epiboly of
Cell-type-specific mRNA transcription and degradation kinetics in zebrafish embryogenesis from metabolically labeled scRNAseq
<p><span>During embryonic development, pluripotent cells assume specialized identities by adopting particular gene expression profiles. However, systematically dissecting the relative contributions of mRNA transcription and degradation to shaping those profiles remains challenging, especially within embryos with diverse cellular identities.<span> Here, we </span>combine<span> </span>single-cell RNA-Seq and metabolic labeling to capture temporal cellular transcriptomes of zebrafish embryos where newly-transcribed (zygotic) and pre-existing (maternal) mRNA can be distinguished. We then introduce kinetic models to quantify mRNA transcription and degradation rates within individual cell types during their specification. These models reveal highly varied regulatory rates across thousands of genes, coordinated transcription and destruction rates for many transcripts, and link differences in degradation to specific sequence elements. They also identify cell-type-specific differences in degradation, namely selective retention of maternal transcripts within primordial germ cells and enveloping layer cells, two of the earliest specified cell-types. Our study provides a quantitative approach to study mRNA regulation during</span> a dynamic spatio-temporal response<span>.</span></p> <p> </p> <p>This repository contains the raw microscopy data that is analyzed in Figures 6F-I and Supplementary Figure S4 B-D.</p>
Figs 1-2 in Body pigmentation during embryogenesis first found in stoneflies: a case of Megaperlodes niger Yokoyama, Isobe & Yamamoto, 1990 (Insecta: Plecoptera, Perlodidae)
Figs 1-2 – Female adults of Megaperlodes niger. 1, Habitus, dorsal view; 2, Habitus, ventral view.
Increasingly efficient chromatin binding of cohesin and CTCF supports chromatin architecture formation during zebrafish embryogenesis
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Physically asymmetric division of the C. elegans zygote ensures invariably successful embryogenesis
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Data from: The dynamic transmission of positional information in stau-mutants during Drosophila embryogenesis.
It has been suggested that Staufen (Stau) is key in controlling the variability of the posterior boundary of the Hb anterior domain (xHb). However, its underlying mechanism is elusive. Here, we quantified the dynamic 3D expression of segmentation genes in Drosophila embryos. With improved control of measurement errors, we show xHb of stau- mutants reproducibly moves posteriorly by 10% of the embryo length (EL) to the wild type (WT) position in the nuclear cycle (nc) 14, and its variability at short time windows is comparable as that of the WT. Moreover, for stau- mutants, the upstream Bicoid (Bcd) gradients show equivalent relative intensity noise to that of the WT in nc12-nc14, and the downstream Even-skipped (Eve) and cephalic furrow (CF) show the same positional errors as the WT. Our results indicate that threshold-dependent activation and self-organized filtering are not mutually exclusive but could both be implemented in early Drosophila embryogenesis.
Oxygen consumption of juvenile brown trout, Salmo trutta, under varying thermal conditions during embryogenesis
<p>Climate change is predicted to increase the future thermal conditions in northern latitudes with the potential effect of altering the metabolic scope and potential fitness of aquatic ectotherms. We experimentally tested the effect of elevated egg incubation temperature on the metabolic scope in juvenile brown trout (<i>Salmo trutta</i>). Brown trout cohorts from anadromous and resident crosses were raised from egg through exogenous feeding of juveniles in either natural river temperatures (cold) or elevated (+ 3 °C, warm) temperatures. In respirometry studies, we measured oxygen consumption rates of juvenile trout from both incubation temperatures and all possible breeding crosses after they were feeding exogenously and at an ambient temperature of 13 °C. These measures were taken over a period where the trout were resting, allowing for the determination of standard metabolic rate (SMR), and followed by a chase to exhaustion allowing for the measure of the maximum metabolic rate (MMR). The aerobic scope (AS) of these juveniles from four anadromous-resident crosses and from both incubation temperatures could then be calculated as AS = MMR – SMR. This dataset represents a key to all respiration trials including: fish embryonic incubation temperature, parental cross, fish total length (mm), calculated mass (g) per fish, ventilation rate (opercular beats/min), and water temperature per respiration trial. For each fish tested, the dissolved oxygen levels in the respirometry chamber were recorded continuously through periods of static respiration and recharge flow in 15 sec intervals for the duration of the trial testing periods (~ 5-7 hrs). These data were evaluated for SMR and MMR using a respiration program (respR) in the R statistical program.</p>
Temperature regime during embryogenesis alters subsequent behavioural phenotypes of juvenile brown trout
<p><span>Climate warming imposes a serious threat, especially to freshwater ecosystems in temperate and (sub)polar regions, which are often dominated by cold-adapted ectotherms. Although relatively intense warming during winter is common across the climatic regions, comparably little focus has been put on the organismal impacts of winter warming. Embryonic development, which is exceptionally susceptible to ambient temperature, occurs during winter in various freshwater ectotherms. Yet, our knowledge of the effects of increased temperature during embryogenesis on later life stages is limited. Using brown trout (<em>Salmo trutta</em>), we examined how a 1.5°C temperature increase from fertilisation to hatching affects various traits at the onset of the free-swimming stage (i.e., a</span><span> compa</span><span>rison </span><span>between 3.5°C or 5.0°C treatments). Although all hatchlings were kept at the same temperature (7.0</span><span>°C)</span><span> from hatching to the onset of the free-swimming stage for about two months, the temperature increase during embryogenesis substantially reduced key ecological behaviours, i.e., activity and exploration levels, at the onset of the free-swimming stage despite only marginal temperature effects on morphological and physiological traits at this stage. Given the importance of behavioural traits in early growth and survival, our study suggests a likely pathway through which subtle changes in mean winter temperature affect early fitness.</span></p>
Fig. 4 in Direct somatic embryogenesis of drought resistance pistachio (Pistacia vera L.) and expression analysis of somatic embryogenesis-related genes
Fig. 4. Somatic embryos induction of P. vera L. Sarakhs variety from immature zygotic embryos under dark condition after 60 days. Somatic embryos exhibited by arrows (a) Somatic embryos in globular stage, (b) Somatic embryos in heart stage, (c) Cotyledonary somatic embryos. Bar 2 mm.
Fig. 6 in Direct somatic embryogenesis of drought resistance pistachio (Pistacia vera L.) and expression analysis of somatic embryogenesis-related genes
Fig. 6. Secondary somatic embryos produced from primay somatic embryos of P. vera L. Sarakhs variety. Bar 2 mm.
Fig. 8 in Direct somatic embryogenesis of drought resistance pistachio (Pistacia vera L.) and expression analysis of somatic embryogenesis-related genes
Fig. 8. Relative expression of selected embryogenesis-related genes on embryonic and non-embryonic samples of two genotypes (a: Sarakhs variety and b: Ghazvini cultivar) of P.vera L in three technical replication.ACT was used as a reference gene for data normalization.Mean value and standard deviation (SD) were presented for three biological replicates.The significance differences (p <.01) and non-significant differences showed with two stars and "ns", respectively.
Data from: Effects of radiation from contaminated soil and moss in Fukushima on embryogenesis and egg hatching of the aphid Prociphilus oriens
Radiation-contaminated soils are widespread around the Fukushima Daiichi Nuclear Power Plant, and such soils raise concerns over its harmful effect on soil-dwelling organisms. We evaluated the effects of contaminated soil and moss sampled in Fukushima on the embryogenesis and hatching of aphid eggs, along with the measurement of the egg exposure dose. Cs-137 concentration in soil and moss from Fukushima ranged from 2200 to 3300 Bq/g and from 64 to 105 Bq/g, respectively. Eggs of the eriosomatine aphid Prociphilus oriens that were collected from a non-contaminated area were directly placed on the soil and moss for 4 or 3 months during diapause and then incubated until hatching. The total exposure dose to the eggs was estimated as ca. 100–200 mGy in the 4-month soil experiment and 4–10 mGy in the 4-month moss experiment. There was no significant difference in egg hatchability between the contaminated soil treatment and the control. No morphological abnormalities were detected in the first instars that hatched from the contaminated soil treatment. However, we found weak effects of radiation on egg hatching; eggs placed on the contaminated moss hatched earlier than did the control eggs. On the contaminated soil, the effects of radiation on egg hatching were not obvious because of uncontrolled environmental differences among containers. The effects of radiation on egg hatching were detected only in containers where high hatchability was recorded. Through the experiments, we concluded that the aphid eggs responded to ultra-low-dose radiation by advancing embryogenesis.
Figure 6. Stage 3, about 48–50 h in Comparative investigation of the late embryogenesis of Leptodora kindtii (Focke, 1844) (Crustacea: Branchiopoda), with notes on types of embryonic development and larvae in Cladocera
Figure 6. Stage 3, about 48–50 h development: (A) general view of embryo, dorsal side; (B) rudiments of carapace and second antenna; (C) rudiments of postabdominal claws; (D) general view, ventral side; (E) head of embryo; (F) maxillae I and mandibles. Scale bars: (A, D) 50 mm; (B, C, E, F) 25 mm.
Figure 5. Stage 2, about 45–47 h in Comparative investigation of the late embryogenesis of Leptodora kindtii (Focke, 1844) (Crustacea: Branchiopoda), with notes on types of embryonic development and larvae in Cladocera
Figure 5. Stage 2, about 45–47 h development: (A) general view of embryo, dorsal side; (B) posterior end of embryo; (C) rudiments of thoracic limbs; (D) rudiments of mandibles and maxillae I; (E) ovary. Scale bars: (A) 100 mm; (B–E) 50 mm.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.