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272 results for “endothelium”
Dataset related to article "Molecular Studies and ex vivo Complement assay on Endothelium Highlight the Genetic Complexity of Atypical Hemolytic Uremic Syndrome: The Case of a Pedigree With a Null CD46 Variant".
<p><em>The files contain raw data related to the article "Molecular Studies and ex vivo Complement assay on Endothelium Highlight the Genetic Complexity of Atypical Hemolytic Uremic Syndrome: The Case of a Pedigree With a Null CD46 Variant", available from <a href="https://www.frontiersin.org/articles/10.3389/fmed.2020.579418/full">https://www.frontiersin.org/articles/10.3389/fmed.2020.579418/ful</a>l.</em></p> <p>File <strong>"Genetic and clinical data"</strong>:</p> <ul> <li>In the sheet "485 aHUS patients" are reported data obtained from the screening of 485 unrelated patients with aHUS including rare variants (RVs) in complement disease-associated genes (<em>CFH, CD46, CFI, C3, CFB </em>and <em>THBD</em>), the presence of <em>CFH-CFHR</em> genomic rearrangements and/or anti-FH antibodies.</li> <li>In the sheet "Pedigrees with c.286+2T>G" are listed all pedigrees carrying the c.286+2T>G variant, the diseases status of all subjects and the age of disease onset of patients. In bold are indicated pedigrees (n=7) used to study the penetrance of aHUS in c.286+2T>G carriers.</li> <li>In the sheet "Haplotypes" are reported genotypes used to evaluate the association between the presence of <em>CFH-H3</em> and <em>CD46<sub>GGAAC</sub></em> risk haplotypes and aHUS. Results of this analysis are reported in Table 3 of the published paper.</li> <li>In the sheet "Raw data Fig.2" are reported data of "platelet count" and "serum creatinine" of the proband used to elaborate Figure 2.</li> </ul> <p>In the file <strong>"C3 and C5b-9 deposition"</strong> is reported the quantification of serum-induced C3 and C5b-9 deposition on human microvascular endothelial cell line (HMEC-1). The fluorescent staining was evaluated with Image J and expressed as pixel<sup>2 </sup>per field analyzed. The fields with the lowest and highest values were excluded from calculation. These values were used to elaborate data included in Table 2 and in Figure 5.</p> <p>In the file <strong>"CD46 protein expression"</strong> are reported data of CD46 expression on peripheral blood mononuclear cells (PBMCs) isolated from the proband, his relatives and healthy volunteers. Data of specific expression of CD46 (evaluated for SCR1 or for SCR4 as reported in the materials and methods section) are indicated as median fluorescence intensity (MFI) percentage compared with the control.</p> <p>In the ppt file <strong>"cDNA amplification and sequencing results"</strong> is reported:</p> <ul> <li>the agarose gel image of the amplified cDNA from the control (ctr), the proband (IV-8) and his healthy father (III-7).</li> <li>Electropherograms obtained from the cDNA sequencing of the control (ctr), the proband (IV-8) and his healthy father (III-7).</li> </ul> <p>Additional data will be made available by the authors, without undue reservation, to any qualified researcher. </p>
Tuning apicobasal polarity and junctional recycling in the hemogenic endothelium orchestrates the morphodynamic complexity of emerging pre-hematopoietic stem cells —Source data 4 relative to Figure 7 – ArhGEF11 CRISPR interference
<p><span>Raw image files (TIFF format), corresponding 2D-cartographies (_2Dmap.tiff files) and metadata files for 2D-cartographies (.xml files, readable with the opensource software Icy), relative to <strong>Figure 7B </strong>and<strong> Figure 7 - Figure Supplement 6</strong> (see <strong>Materials and Methods — Morphological and morphometric analysis of aortic and hemogenic cells</strong>).</span></p> <p><span>The source data comprises for each 48 - 55 hpf <em>Tg(kdrl:eGFP-JAM3b; kdrl:nls-mKate2)</em> zebrafish embryo 3 z-stack and 2D cartographies (segments 1 to 3) encompassing the whole length of the aorta, for control condition (n = 2 individuals) and morpholino splicing interference condition (n = 2 individuals). For z-stacks of both control and morphant conditions, two fluorescence channels were acquired, corresponding to the nuclear mKate2 expressed in endothelial cells and the eGFP-JAMs signal localized at the intercellular junctions of endothelial cells. Z-stack were acquired using a confocal spinning disk microscope. Voxel size: x: 0.1635, y: 0.1635, z:0.3 µm. 2D-cartographies were obtained using the Icy plugin “TubeSkinner”, and the semi-manual segmentation of all aortic cells can be uploaded from the corresponding metadata file on the 2D-cartographies using the load ROI function of Icy.</span></p>
Tuning apicobasal polarity and junctional recycling in the hemogenic endothelium orchestrates the morphodynamic complexity of emerging pre-hematopoietic stem cells —Source data 3 relative to Figure 7 – ArhGEF11 morpholino splicing interference
<p><span>Raw image files (TIFF format), corresponding 2D-cartographies (_2Dmap.tiff files) and metadata files for 2D-cartographies (.xml files, readable with the opensource software Icy), relative to <strong>Figure 7A </strong>and<strong> Figure 7 - Figure Supplement 5</strong> (see <strong>Materials and Methods — Morphological and morphometric analysis of aortic and hemogenic cells</strong>).</span></p> <p><span>The source data comprises for each 48 - 55 hpf <em>Tg(kdrl:eGFP-JAM2a; kdrl:nls-mKate2)</em> zebrafish embryo 3 z-stack and 2D cartographies (segments 1 to 3) encompassing the whole length of the aorta, for control condition (n = 2 individuals) and morpholino splicing interference condition (n = 3 individuals). For z-stacks of both control and morphant conditions, two fluorescence channels were acquired, corresponding to the nuclear mKate2 expressed in endothelial cells and the eGFP-JAMs signal localized at the intercellular junctions of endothelial cells. Z-stack were acquired using a confocal spinning disk microscope. Voxel size: x: 0.1635, y: 0.1635, z:0.3 µm. 2D-cartographies were obtained using the Icy plugin “TubeSkinner”, and the semi-manual segmentation of all aortic cells can be uploaded from the corresponding metadata file on the 2D-cartographies using the load ROI function of Icy.</span></p>
Tuning apicobasal polarity and junctional recycling in the hemogenic endothelium orchestrates the morphodynamic complexity of emerging pre-hematopoietic stem cells —Source data 1 relative to Figure 3
<p><span>Raw image files (TIFF format) relative <strong>to Figure 3</strong> (see <strong>Materials and Methods — Dt-runx1 phenotype analysis – cell count</strong>).</span></p> <p><span>The source data comprises for each 52 - 55 hpf zebrafish embryo 3 z-stack (segments 1 to 3) encompassing the whole length of the aorta, for control condition (<em>Tg(Kdrl:Gal4;UAS:RFP), </em>n = 3 individuals) and mutant condition (<em>Tg(kdrl:Gal4;UAS:RFP;4xNR:dt-runx1-eGFP), </em>n = 7 individuals). For control condition, one fluorescence channel was acquired, corresponding to the cytoplasmic RFP expressed in endothelial cells. For mutant condition, two fluorescence channels were acquired, corresponding first to the cytoplasmic RFP expressed in endothelial cells using the same reporter as for the control condition, and second the cleaved cytoplasmic GFP reporting the expression of our dt-runx1 mutant construct in endothelial cells. Z-stack were acquired using a confocal spinning disk microscope. Voxel size: x: 0.1635, y: 0.1635, z:0.3 µm.</span></p>
Tuning apicobasal polarity and junctional recycling in the hemogenic endothelium orchestrates the morphodynamic complexity of emerging pre-hematopoietic stem cells —Source data 2 relative to Figure 4
<p><span>Raw image files (TIFF format) and segmented 3D images (.ims, Imaris proprietary files) relative to <strong>Figure 4</strong> and <strong>Figure 4 Figure Supplement 3</strong> (see <strong>Materials and Methods — RNAscope image analysis – Pard3</strong>).</span></p> <p><span>The source data comprises for each 52 - 55 hpf zebrafish embryos 2 z-stack (segments 1 to 2) encompassing the whole length of the aorta, for control condition (<em>Tg(Kdrl:eGFP), </em>n = 7 individuals) and mutant condition (<em>Tg(kdrl:Gal4; 4xNR:dt-runx1-eGFP), </em>n = 12 individuals). For both control and mutant conditions, two fluorescence channels are displayed, corresponding to the cytoplasmic GFP expressed in endothelial cells (in green) and the RNAscope signal (OPAL-570, in magenta). Z-stack were acquired using a confocal spinning disk microscope. Voxel size: x: 0.1635, y: 0.1635, z:0.4 µm. The .ims files contain the 3D rendering of the z-stacks as well as the segmentations of Pard3ba mRNA RNAscope spots (in magenta), in the aorta (Spots 1 Selection) or outside (Spots 1), as well as the segmentation of endothelial cells (green) (Cells 1) and hemogenic endothelial cells (Cells 1 Cell export).</span></p>
Text-fig. 17. Scanning electron microscope (SEM) and synchrotron radiation X-ray tomographic microscopy (SRXTM) images of a fruit of Canrightia elongata sp. nov. (a–g) and isolated Canrightia-like seeds (h–j); Torres Vedras locality, Portugal. a–c) Holotype; fruit in lateral view showing four fused tepals at the base (c, upper arrowheads) and prominent cavities in the fruit wall formed by scattered oil bodies and possible subtending bract (c, lower arrowhead); d) Transverse section (SRXTM orthoslice xy1510) through the fruit showing three locules, one with the remains of the endothelium (top left, 1), the other two (2, 3) with remains of presumed endosperm tissue; note that the locule to the right (3) is crushed; e, f) Radial longitudinal (e; SRXTM orthoslice xz1212) in The Early Cretaceous Mesofossil Flora Of Torres Vedras (Ne Of Forte Da Forca), Portugal: A Palaeofloristic Analysis Of An Early Angiosperm Community
Text-fig. 17. Scanning electron microscope (SEM) and synchrotron radiation X-ray tomographic microscopy (SRXTM) images of a fruit of Canrightia elongata sp. nov. (a–g) and isolated Canrightia-like seeds (h–j); Torres Vedras locality, Portugal. a–c) Holotype; fruit in lateral view showing four fused tepals at the base (c, upper arrowheads) and prominent cavities in the fruit wall formed by scattered oil bodies and possible subtending bract (c, lower arrowhead); d) Transverse section (SRXTM orthoslice xy1510) through the fruit showing three locules, one with the remains of the endothelium (top left, 1), the other two (2, 3) with remains of presumed endosperm tissue; note that the locule to the right (3) is crushed; e, f) Radial longitudinal (e; SRXTM orthoslice xz1212)
Text-fig. 3. Synchrotron radiation X-ray tomographic microscopy (SRXTM) images of fruits of Canrightia foveolata sp. nov.; Catefica locality, Portugal. a) Volume rendering of fruit showing prominent rim around the middle of the fruit with reduced tepals (arrowheads) and partly abraded fruit wall exposing the pitted endotesta surface of one of two seeds (arrow); note two of the vascular bundles (vb) extending from the base of the fruit to the tepals; b) Voltex of fruit showing prominent rim around the fruit (arrowhead) and dense precipitation of crystals in the endothelium cells of one of the two seeds in the fruit; c) Longitudinal section of fruit (orthoslice yz0520) showing the inferred hypanthium rim (arrow head) and two seeds, one with a dense precipitation of crystals; note the prominent endothelium cells (asterisks) of the inner integument and the well-developed fruit wall above the seeds; d) Transverse section through basal part of fruit and seeds close to the micropyle (orthoslice xy0312) showing partly abraded fruit wall with five vascular bundles (vb) and details of the seed coat with endotesta (oi-end) surrounding the tegmen consisting of an outer epidermis (ii-o), middle layer (ii-m) and a distinct inner epidermis (endothelium) consisting of radially elongated cells (asterisk); e) Transverse section (orthoslice xy1680) through apical part of the fruit close to chalaza showing the tips of two seeds; note the endotesta (oi-end) surrounded by thick-walled cells of the exotesta (oi-o); f) Transverse section (orthoslice xy1485) through fruit in the region of the hypanthium rim showing sections through the two seeds close to the chalazal region; note endotesta (oi-end) surrounded by larger cells of exotesta (oi-o) and fruit wall (fr). Specimen, Catefica 49-S174249 (holotype, a–f). Scale bars = 300 Μm (a–c, e, f), 100 Μm (d). in The Early Cretaceous Mesofossil Flora Of Catefica, Portugal: Angiosperms
Text-fig. 3. Synchrotron radiation X-ray tomographic microscopy (SRXTM) images of fruits of Canrightia foveolata sp. nov.; Catefica locality, Portugal. a) Volume rendering of fruit showing prominent rim around the middle of the fruit with reduced tepals (arrowheads) and partly abraded fruit wall exposing the pitted endotesta surface of one of two seeds (arrow); note two of the vascular bundles (vb) extending from the base of the fruit to the tepals; b) Voltex of fruit showing prominent rim around the fruit (arrowhead) and dense precipitation of crystals in the endothelium cells of one of the two seeds in the fruit; c) Longitudinal section of fruit (orthoslice yz0520) showing the inferred hypanthium rim (arrow head) and two seeds, one with a dense precipitation of crystals; note the prominent endothelium cells (asterisks) of the inner integument and the well-developed fruit wall above the seeds; d) Transverse section through basal part of fruit and seeds close to the micropyle (orthoslice xy0312) showing partly abraded fruit wall with five vascular bundles (vb) and details of the seed coat with endotesta (oi-end) surrounding the tegmen consisting of an outer epidermis (ii-o), middle layer (ii-m) and a distinct inner epidermis (endothelium) consisting of radially elongated cells (asterisk); e) Transverse section (orthoslice xy1680) through apical part of the fruit close to chalaza showing the tips of two seeds; note the endotesta (oi-end) surrounded by thick-walled cells of the exotesta (oi-o); f) Transverse section (orthoslice xy1485) through fruit in the region of the hypanthium rim showing sections through the two seeds close to the chalazal region; note endotesta (oi-end) surrounded by larger cells of exotesta (oi-o) and fruit wall (fr). Specimen, Catefica 49-S174249 (holotype, a–f). Scale bars = 300 Μm (a–c, e, f), 100 Μm (d).
Text-fig. 2. Scanning electron microscope (SEM, a, c–f) and synchrotron radiation X-ray tomographic microscopy (SRXTM, b, g) images of the flower of Mugideiriflora portugallica (a, b) and fruits, seeds and pollen of Canrightia resinifera (c–g); Catefica locality, Portugal. a) Oblique, apical view of flower showing multiparted organization with numerous laminar tepals, stamens that are rhomboidal and flattened in transverse section and carpels that are borne on the short conical apex of the receptacle; b) Transverse section (orthoslice xy0800) through basal part of flower showing the elongate bases of the laminar tepals and the flattened rhomboidal bases of the stamens; c) Fruit in lateral view showing irregular surface resulting from the abundant resin bodies in the fruit and hypanthium wall, scars from stamens on the rim of the hypanthium (arrowheads) and the lobed apical stigmatic region (st); d) Broken fruit with one or two seeds missing but showing three pendant, orthotropous seeds with pointed micropylar regions (mi) and a finely pitted crystalliferous endotesta; note the remains of the apical vascular bundles (vb); e) Single seed isolated from a fruit showing two distinct bundles (vb) still attached apically to the chalazal region of the seed, the pointed micropyle (mi) and the finely pitted surface of the crystalliferous endotesta; f) Monocolpate pollen from stigmatic region of fruit showing the long colpus and coarse reticulum; g) Transverse section (orthoslice xy0705) through a fruit showing four seeds all with radially elongated endothelium cells formed from the inner epidermis of the tegmen (asterisks). Specimens, Catefica 150-S174254 (a, b), Catefica 49-S170377 (c), Catefica 49-S170372 (d), Catefica 50-S170401 (e), Catefica 50-S170404 (f), Catefica 50-S174906 (g). Scale bars = 300 Μm (a–e, g), 6 Μm (f). in The Early Cretaceous Mesofossil Flora Of Catefica, Portugal: Angiosperms
Text-fig. 2. Scanning electron microscope (SEM, a, c–f) and synchrotron radiation X-ray tomographic microscopy (SRXTM, b, g) images of the flower of Mugideiriflora portugallica (a, b) and fruits, seeds and pollen of Canrightia resinifera (c–g); Catefica locality, Portugal. a) Oblique, apical view of flower showing multiparted organization with numerous laminar tepals, stamens that are rhomboidal and flattened in transverse section and carpels that are borne on the short conical apex of the receptacle; b) Transverse section (orthoslice xy0800) through basal part of flower showing the elongate bases of the laminar tepals and the flattened rhomboidal bases of the stamens; c) Fruit in lateral view showing irregular surface resulting from the abundant resin bodies in the fruit and hypanthium wall, scars from stamens on the rim of the hypanthium (arrowheads) and the lobed apical stigmatic region (st); d) Broken fruit with one or two seeds missing but showing three pendant, orthotropous seeds with pointed micropylar regions (mi) and a finely pitted crystalliferous endotesta; note the remains of the apical vascular bundles (vb); e) Single seed isolated from a fruit showing two distinct bundles (vb) still attached apically to the chalazal region of the seed, the pointed micropyle (mi) and the finely pitted surface of the crystalliferous endotesta; f) Monocolpate pollen from stigmatic region of fruit showing the long colpus and coarse reticulum; g) Transverse section (orthoslice xy0705) through a fruit showing four seeds all with radially elongated endothelium cells formed from the inner epidermis of the tegmen (asterisks). Specimens, Catefica 150-S174254 (a, b), Catefica 49-S170377 (c), Catefica 49-S170372 (d), Catefica 50-S170401 (e), Catefica 50-S170404 (f), Catefica 50-S174906 (g). Scale bars = 300 Μm (a–e, g), 6 Μm (f).
April 7, 2024 (v1) Image Open Tuning apicobasal polarity and junctional recycling in the hemogenic endothelium orchestrates the morphodynamic complexity of emerging pre-hematopoietic stem cells —Source data 5 relative to Figure 7 - Figure Supplement 4
<p>Source data file relative to <strong><span>Figure 7 – figure supplement 4 Panel A</span></strong></p> <p><span>Raw image of agarose gel showing the 2 alternative mRNAs encoding for ArhGEF11 in control animals (left track, control) and after injection of the MO at the one cell stage (right track, +MO at 2 and 5ng). The source data includes the raw files (native format .scn and open source format .tiff) as well as a pdf file showing both the full scale image and the cropped image selected for the figure.<br></span></p>
Effect of Dietary Sodium Intake on Vascular Endothelium
ClinicalTrials.gov study NCT01550315. IPD Sharing: Not stated. Countries: 1. Publications: 26.
Uric Acid and the Endothelium in CKD
ClinicalTrials.gov study NCT01228903. IPD Sharing: YES. Countries: 1. Publications: 1.
Evaluation of JAK-STAT inhibition in JAK2V617F myeloproliferative leukocytes and endothelium
Open the record for dataset details and reuse information.
Safety of Trypan Blue on Endothelium of Diabetic Retinopathy Patients
ClinicalTrials.gov study NCT03755752. IPD Sharing: NO. Countries: 1. Publications: 1.
Qualitative and Quantitative Endothelium Changes After Cataract Surgery: Ultrasound Phacoemulsification vs Nanolaser Technique
ClinicalTrials.gov study NCT05886283. IPD Sharing: UNDECIDED. Countries: 1. Publications: 1.
Effects of COVID-19 on Endothelium in HIV-Positive Patients in Sub-Saharan Africa
ClinicalTrials.gov study NCT04709302. IPD Sharing: UNDECIDED. Countries: 2. Publications: 1.
Blood Endothelium Biomarkers to Predict Major Adverse Cardiovascular Events After Percutaneous Coronary Intervention
ClinicalTrials.gov study NCT00725868. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Vildagliptin and Endothelium-dependent Vasodilatation
ClinicalTrials.gov study NCT01000688. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Anticancer Treatment of Breast Cancer Related to Cardiotoxicity and Dysfunctional Endothelium
ClinicalTrials.gov study NCT02652975. IPD Sharing: NO. Countries: 1. Publications: 1.
Endothelium Dependent and Non-dependent Vasodilatation in Human Gingiva
ClinicalTrials.gov study NCT04918563. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Effect of Apple Polyphenols on Vascular Oxidative Stress and Endothelium Function Study (APP trial_2016)
ClinicalTrials.gov study NCT03053986. IPD Sharing: UNDECIDED. Countries: 1. Publications: 1.
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