Find research datasets worth reusing

opencc-by-4.0Apr 2023View details →

Enhanced Biosafety of the Sleeping Beauty Transposon System by Using mRNA as Source of Transposase to Efficiently and Stably Transfect Retinal Pigment Epithelial Cells

Raw data of the publication "Enhanced Biosafety of the Sleeping Beauty Transposon System by Using mRNA as Source of Transposase to Efficiently and Stably Transfect Retinal Pigment Epithelial Cells". Abstract:  Neovascular age-related macular degeneration (nvAMD) is characterized by choroidal neovascularization (CNV), which leads to retinal pigment epithelial (RPE) cell and photoreceptor degeneration and blindness if untreated. Since blood vessel growth is mediated by endothelial cell growth factors, including vascular endothelial growth factor (VEGF), treatment consists of repeated, often monthly, intravitreal injections of anti-angiogenic biopharmaceuticals. Frequent injections are costly and present logistic difficulties; therefore, our laboratories are developing a cell-based gene therapy based on autologous RPE cells transfected ex vivo with the pigment epithelium derived factor (PEDF), which is the most potent natural antagonist of VEGF. Gene delivery and long-term expression of the transgene are enabled by the use of the non-viral Sleeping Beauty (SB100X) transposon system that is introduced into the cells by electroporation. The transposase may have a cytotoxic effect and a low risk of remobilization of the transposon if supplied in the form of DNA. Here, we investigated the use of the SB100X transposase delivered as mRNA and showed that ARPE-19 cells as well as primary human RPE cells were successfully transfected with the Venus or the PEDF gene, followed by stable transgene expression. In human RPE cells, secretion of recombinant PEDF could be detected in cell culture up to one year. Non-viral ex vivo transfection using SB100X-mRNA in combination with electroporation increases the biosafety of our gene therapeutic approach to treat nvAMD while ensuring high transfection efficiency and long-term transgene expression in RPE cells.

opencc-by-4.0Nov 2024View details →

Inhibition of epithelial cell YAP-TEAD/LOX signaling attenuates pulmonary fibrosis "

<table> <tbody> <tr> <td> Idiopathic pulmonary fibrosis (IPF) is a progressive and lethal disease characterized by excessive extracellular matrix (ECM) deposition. Current IPF therapies slow disease progression but do not stop or reverse it. The (myo)fibroblasts are thought to be the main cellular contributors to excessive ECM production in IPF. Here we report that fibrotic AT2 cells regulate production and crosslinking of ECM via the co-transcriptional activator YAP. YAP leads to increase expression of Lysyloxidase (LOX) and subsequent LOX mediated crosslinking by fibrotic AT2 cells. Pharmacological YAP inhibition reverses fibrotic AT2 cell reprogramming and LOX expression in experimental lung fibrosis in vivo and in human fibrotic tissue ex vivo. We thus identify YAP-TEAD/LOX inhibition in AT2 cells as a promising potential new therapy for IPF patients.  In </td> </tr> </tbody> </table>

opencc-by-4.0Jan 2024View details →

Datasets: Natural killer cells associate with malignant epithelial cells in the pancreatic ductal adenocarcinoma tumor microenvironment

The following are necessary data files for the manuscript "Natural killer cells associate with malignant epithelial cells in the pancreatic ductal adenocarcinoma tumor microenvironment": <ul> <li>.zip files for TMA_1, TMA_2, TMA_3, and TMA_4 are .mcd files acquired from imaging mass cytometry (IMC) for each slide of the pancreas TMA slide series</li> <li>pancreas_TMA_sample_info.xlxs includes info on all samples of the TMA slide series that were imaged by IMC</li> <li>custom_gates_0.zip includes histoCAT-derived single cell data files from all IMC samples in the pancreas TMA to be used for single cell analyses in R</li> <li>PDAC_IMC.RDS is a Seurat object of the IMC-derived PDAC single cell data to use for single cell and spatial analyses</li> <li>PDAC_sce is a SingleCellExperiment object of IMC-derived PDAC single cell data to use for spatial analyses</li> <li>mat.RDS is a distance matrix of PDAC cell types to use in R to generate network graph (Figure 2)</li> </ul>

opencc-by-4.0Sep 2021View details →

GTEx analysis for the paper entitled: The histone variant H2A.J is enriched in luminal epithelial gland cells

H2A.J is a poorly studied mammalian-specific variant of histone H2A. We used immunohistochemistry to study its localization in various human and mouse tissues. H2A.J showed cell-type specific expression with a striking enrichment in luminal epithelial cells of multiple glands including those of breast, prostate, pancreas, thyroid, stomach, and salivary glands. H2A.J was also highly expressed in many carcinoma cell lines and in particular, those derived from luminal breast and prostate cancer. H2A.J thus appears to be a novel marker for luminal epithelial cancers. Knocking-out the H2AFJ gene in T47D luminal breast cancer cells reduced the expression of several estrogen-responsive genes which may explain its putative tumorigenic role in luminal-B breast cancer.

opencc-by-4.0Nov 2024View details →

Time-lapse 3D confocal microscopy videos of mitochondrial dynamics in human alveolar epithelial cells (A549-DsRed) infected with Mycobacterium marinum (Mmar) strains

<div>The dataset consists of time-lapse, 3D confocal images of mitochondrial dynamics in human alveolar epithelial cells (A549-DsRed) infected with Mycobacterium marinum (Mmar) strains. Images were captured at 60X magnification in an environmental chamber at 35°C for live-cell imaging. Host cell mitochondria were labeled with red fluorescent protein (RFP) and infected with both wildtype (wt) and ESAT-6 operon knockout mutant labeled with green fluorescent protein (GFP) at MOI of 100 for 24 hours at 35°C. Infected cells were identified and analyzed to explore the effect of pathogenic mycobacteria on mitochondrial morphology over time.</div> <div> </div> <div>More details available in this preprint: <a href="https://doi.org/10.48550/arXiv.2411.06035">https://doi.org/10.48550/arXiv.2411.06035</a></div>

opencc-by-4.0Sep 2021View details →

Mammary single-cell RNA-seq analysis and prostate cancer survival as a function of H2AFJ expression for the paper entitled: The histone variant H2A.J is enriched in luminal epithelial cells

opencc-by-4.0May 2023View details →

The First Transcriptomic Atlas of the Adult Lacrimal Gland Reveals Epithelial Complexity and Identifies Novel Progenitor Cells in Mice

This project contains the R objects and code to reproduce the analyses and figures presented in the research article: 'The First Transcriptomic Atlas of the Adult Lacrimal Gland Reveals Epithelial Complexity and Identifies Novel Progenitor Cells in Mice.' Cells 2023, 12, 1435. https://doi.org/10.3390/cells12101435 Raw data (FASTQ files and CellRanger output files used for the preprocessing of individual datasets) can be found on Gene Expression Omnibus database (www.ncbi.nlm.nih.gov/geo/) under accession # GSE232146.

opencc-by-4.0Aug 2023View details →

Predictive nano-QSAR modeling of the cytotoxicity using epithelial cells obtained from Chinese hamster ovary (CHO-K1 cell line) for hybrid TiO2-based nanomaterials

Results obtained from developed model indicated that the cytotoxicity of hybrid TiO2-based nanomaterials is related to additive electronegativity (χmix) of studied nanomaterials that are indirectly related to the electron generation and ROS formation. ROS production is the most common toxicity cause as discussed in the literature in the case of nanoparticles. The high efficiency of surface modified TiO2-based semiconductors can be attributed to the involvement of TiO2 band gap (Eg) excitation and absence of noble metals at the TiO2 surface. It can be expected that noble metals (i.e. Pd/Pt) may trap holes (h+), at the same time photo-generated electrons can be then transferred from the valence band to the conduction band of TiO2 and to its surface where redox processes were initiated. Thus, observed reduction of the electron–hole pair recombination influences the reactive oxygen species (ROS) formation and the photocatalytic redox process initiation. Since the electronegativity was positively correlated with the cytotoxicity it can be expected that some ions are released from the TiO2 surface easier than others.

opencc-by-4.0Nov 2020View details →

Dataset - A lung-on-chip model reveals an essential role for alveolar epithelial cells in controlling bacterial growth during early M. tuberculosis infection

Description of the sub-folders Name, type of data, corresponding Figure in the manuscript 3D view of the LoC model - .tiff image stack, Figure 1. Bacterial Growth Rate Data  - .tiff image stacks, .csv files and MATLAB code to extract the fluorescence intensity over time, Figure 2, Figure 2 - figure supplement 2, Figure 2 - figure supplement 4, Figure 3, Figure 3 - figure supplement 2, Figure 4. AT Characterization - .tiff image stacks and MATLAB code to extract the number and volume of lamellar bodies from the stack of confocal images, Figure 1, Figure 1 - figure supplement 1, Figue 1 - figure supplement 2. AT Infection in LoC model - .tiff image stacks, Figure 2 - figure supplement 1. AT Infection in vivo - .tiff image stacks, Figure 1 - figure supplement 3. Simulations of in vivo infections - .dat files of growth rates in macrophages for the WT and ESX-1 deficient populations and MATLAB code to simulate an infection from this data, Figure 4.

opencc-by-4.0Dec 2021View details →

SBF-SEM datasets related to the manuscript "Trans-cellular tunnels induced by the fungal pathogen Candida albicans facilitate invasion through successive epithelial cells without host damage" by Lachat et al, 2022.

11 serial block face- scanning electron microscopy (SBF-SEM) datasets described in the manuscript "Trans-cellular tunnels induced by the fungal pathogen Candida albicans facilitate invasion through successive epithelial cells without host damage" by Lachat et al, 2022. Resolution: 10 nm x,y, 100 nm Z. Datasets description and quantification can be found in the Supplementary information.

opencc-by-4.0Aug 2022View details →

F-actin Imaging of Mechanically Compressed Pseudostratified Human Bronchial Epithelial Cells

Overview This dataset includes immunofluorescence microscopy of pseudostratified airway epithelial cells stained for F-actin. Each field of view consists of 3 images visualizing the apical cell boundaries, basal cell boundaries, and basal cell stress fibers. Cell Culture and Treatment Primary human bronchial epithelial cells (from a single donor) were grown on transwells in air-liquid interface culture for 14 days to model a well-differentiated, pseudostratified airway epithelium. Cells were then exposed to mechanical compression (30 cmH2O for 3 hours) mimicking asthmatic bronchoconstriction. Cells were fixed (4% PFA for 30 minutes) at 24, 48, or 72 hours after mechanical compression. Two transwells were collected per condition and timepoint. Immunofluorescence Imaging Fixed transwells were stained for F-actin (Alexa fluor 488-Phalloidin, ThermoFisher Scientific, diluted 1:40, 30 minutes). Transwell membranes were cut from the plastic support and mounted on glass slides. Slides were imaged using a Zeiss Axio Observer Z1 with an apotome module controlled using Zen Blue 2.0 software. Five random fields of view were imaged from each transwell membrane in a z-stack from substrate to apical cell surface. To visualize various planes through the pseudostratified epithelial layer (apical cell boundaries, basal cell boundaries, and basal cell stress fibers), maximum intensity projections were generated from regions of interest through the z-stack. Dataset Each image file contains <ul> <li>Timepoint: 24, 48, or 72 hours after treatment</li> <li>Condition & Well: control (C) or mechanical compression (P) followed by a number indicating the well (1 or 2)</li> <li>Unique Z-stack ID: 3-digit number</li> <li>Miscellaneous note: MIP or MIP_ROI</li> <li>Region of Interest: apical cell boundaries (ACB), basal cell boundaries (BCB), or basal stress fibers (SF)</li> </ul> For example, these 3 maximum intensity projection images came from the same z-stack/field of view. They are from the 72 hour timepoint, mechanical compression, well #2, z-stack #147: <ul> <li>72hr_P2_147_MIP_ACB.tif: apical cell boundaries</li> <li>72hr_P2_147_MIP_ROI_BCB.tif: basal cell boundaries</li> <li>72hr_P2_147_MIP_ROI_SF.tif: basal stress fiber</li> </ul>

opencc-by-4.0Aug 2022View details →

Phase Contrast Time-Lapse and F-actin Imaging of Mechanically Compressed or Irradiated Pseudostratified Human Bronchial Epithelial Cells

Overview This dataset includes phase contrast time-lapse imaging of in vitro pseudostratified airway epithelial cells to visualize their collective cellular migration after exposure to mechanical compression (mimicking bronchoconstriction) or irradiation. Additionally, the cells were fixed and stained for F-actin to visualize the apical cell boundaries, basal cell boundaries, and basal cell stress fibers. Cell Culture and Treatment Primary human bronchial epithelial cells (from a single donor) were grown on transwells in air-liquid interface (ALI) culture for 14 days to model a well-differentiated, pseudostratified airway epithelium. Cells were then exposed to either mechanical compression (30 cmH2O for 3 hours) mimicking asthmatic bronchoconstriction or irradiation (1Gy of ionizing radiation using a RS 2000 Biological Research Irradiator (RadSource) on ALI days 7, 10, and 14). Phase Contrast Time-Lapse Imaging At 24 or 72 hours after final treatment, cells were imaged to visualize collective cellular migration. For each independent experimental replicate (2 transwells per treatment per timepoint), six fields of view per well were imaged every 6 minutes over 1.5 hours. The imaging chamber was supplied with 37°C, 5% CO2, humidified air on a Zeiss Axio Observer Z1 to collect phase contrast images. The image resolution is 0.586 µm/pixel. Immunofluorescence Imaging Cells were fixed (4% PFA for 30 minutes) at 24 or 72 hours after final treatment (and after phase contrast time-lapse imaging). Fixed transwells were stained for F-actin (Alexa fluor 488-Phalloidin, ThermoFisher Scientific, diluted 1:40, 30 minutes). Transwell membranes were cut from the plastic support and mounted on glass slides. Slides were imaged using a Zeiss Axio Observer Z1 with an apotome module controlled using Zen Blue 2.0 software. Five random fields of view were imaged from each transwell membrane in a z-stack from substrate to apical cell surface. To visualize various planes through the pseudostratified epithelial layer (apical cell boundaries, basal cell boundaries, and basal cell stress fibers), maximum intensity projections were generated from regions of interest through the z-stack. The image resolution is 0.293 µm/pixel. Dataset Phase contrast time-lapse movies are provided as *.avi files. Immunofluorescence images are provided as *.tif files. For an individual transwell, the imaging dataset includes: <ul> <li>6 phase contrast time-lapse movies</li> <li>5 immunofluorescence images of apical cell boundaries</li> <li>5 immunofluorescence images of basal cell boundaries</li> <li>5 immunofluorescence images of basal cell stress fibers</li> </ul> Phase contrast time-lapse filenames contain <ul> <li>Donor: U13</li> <li>Timepoint: 24 or 72 hours</li> <li>Treatment & Well: control (C), mechanical compression (P), or irradiation (R); well 1 or 2</li> <li>Field of View: (1) – (6)</li> </ul> Immunofluorescence image filenames contain: <ul> <li>Donor: U13</li> <li>Timepoint: 24 or 72 hours</li> <li>Treatment & Well: control (C), mechanical compression (P), or irradiation (R); well 1 or 2</li> <li>Field of View: 1-5</li> <li>Region of Interest: apical cell boundaries (ACB), basal cell boundaries (BCB), or basal stress fibers (SF)</li> </ul> Phase contrast time-lapse and immunofluorescence from the same transwell will all start with the same “Donor_Timepoint_Treatment/Well...” (i.e. U13_24_C1…). Note that the images from phase contrast and immunofluorescence are not necessarily from matched locations within the transwell and are at different spatial scales. Immunofluorescence images from the same z-stack field of view will start with the same “Donor_Timepoint_Treatment/Well_FieldofView…” (i.e. U13_24_C1_1…).

opencc-by-4.0Aug 2022View details →

Text-fig. 6. Transmitted light microphotographs of permineralized wood from Govone. a, b: cf. Cupressinoxylon sp., radial section, MGPT-PU141105, a – nodular end of ray parenchyma (arrow), b – thick and pitted horizontal walls of ray parenchyma (arrow). c–f: Pinaceae gen. et sp. indet., MGPT-PU141107, c – abnormal discoloration due to ecological disruptions (radial section), d – rays up to 10 cells high, uniseriate, partly biseriate (black arrow), intercellular spaces observed (white arrows) (tangential section), e – large, thick-walled axial resin canal with more than 9 epithelial cells observed, axial resin canal diameter>60 Μm (transverse section), f – spiral thickenings due to compression (white arrow) (radial section). in Remains Of A Subtropical Humid Forest In A Messinian Evaporitebearing Succession At Govone, Northwestern Italy - Preliminary Results

Text-fig. 6. Transmitted light microphotographs of permineralized wood from Govone. a, b: cf. Cupressinoxylon sp., radial section, MGPT-PU141105, a – nodular end of ray parenchyma (arrow), b – thick and pitted horizontal walls of ray parenchyma (arrow). c–f: Pinaceae gen. et sp. indet., MGPT-PU141107, c – abnormal discoloration due to ecological disruptions (radial section), d – rays up to 10 cells high, uniseriate, partly biseriate (black arrow), intercellular spaces observed (white arrows) (tangential section), e – large, thick-walled axial resin canal with more than 9 epithelial cells observed, axial resin canal diameter>60 Μm (transverse section), f – spiral thickenings due to compression (white arrow) (radial section).

opencc-by-4.0Aug 2021View details →

Raw data for the article: Human Amnion Epithelial Cells Impair T Cell Proliferation: The Role of HLA-G and HLA-E Molecules

The immunoprivilege status characteristic of human amnion epithelial cells (hAECs) has been recently highlighted in the context of xenogenic transplantation. However, the mechanism(s) involved in such regulatory functions have been so far only partially been clarified. Here, we have analyzed the expression of HLA-Ib molecules in isolated hAEC obtained from full term placentae. Moreover, we asked whether these molecules are involved in the immunoregulatory functions of hAEC. Human amnion-derived cells expressed surface HLA-G and HLA-F at high levels, whereas the commonly expressed HLA-E molecule has been measured at a very low level or null on freshly isolated cells. HLA-Ib molecules can be expressed as membrane-bound and soluble forms, and in all hAEC batches analyzed we measured high levels of sHLA-G and sHLA-E when hAEC were maintained in culture, and such a release was time-dependent. Moreover, HLA-G was present in extracellular vesicles (EVs) released by hAEC. hAEC suppressed T cell proliferation in vitro at different hAEC:T cell ratios, as previously reported. Moreover, inhibition of T cell proliferation was partially reverted by pretreating hAEC with anti-HLA-G, anti-HLA-E and anti-β2 microglobulin, thus suggesting that HLA-G and -E molecules are involved in hAEC-mediated suppression of T cell proliferation. Finally, either large-size EV (lsEV) or small-size EV (ssEV) derived from hAEC significantly modulated T-cell proliferation. In conclusion, we have here characterized one of the mechanism(s) underlying immunomodulatory functions of hAEC, related to the expression and release of HLA-Ib molecules.

opencc-by-4.0Apr 2024View details →

Modelling Cyclic Stretch of Patient-Derived Alveolar Epithelial Cells from Organoids using a New Alveoli-on-Chip Platform

The data originates from lung tumor samples obtained at the University Hospital Bern. Patient-derived alveolar epithelial type 2 cells (AEC2) were isolated and cultured into organoids. Grown organoids where dissociated and seeded onto a novel alveoli-on-chip system submerged in medium. After 24 h of either no mechanical stimulus (control) or cyclic breathing stretch (stretch), RNA was isolated and sequenced. The RNA-seq data underwent quality control, alignment to the reference genome, read counting, DGE and GSEA. All analyses were run in R version 4.2.1.

dryad40/100

Force propagation between epithelial cell doublets

Cell-generated forces play a major role in coordinating the large-scale behavior of cell assemblies, in particular during development, wound healing and cancer. Mechanical signals propagate faster than biochemical signals but can have similar effects, especially in epithelial tissues with strong cell-cell adhesion. However, a quantitative description of the transmission chain from force generation in a sender cell, force propagation across cell-cell boundaries, and the concomitant response of receiver cells is missing. For a quantitative analysis of this important situation, here we propose a minimal model system of two epithelial cells on an H-pattern ("cell doublet"). After optogenetically activating RhoA, a major regulator of cell contractility, in the sender cell, we measure the mechanical response of the receiver cell by traction force and monolayer stress microscopies. In general, we find that the receiver cells show an active response so that the cell doublet forms a coherent unit. However, force propagation and response of the receiver cell also strongly depend on the mechano-structural polarization in the cell assembly, which is controlled by cell-matrix adhesion to the adhesive micropattern. We find that the response of the receiver cell is stronger when the mechano-structural polarization axis is oriented perpendicular to the direction of force propagation, reminiscent of the Poisson effect in passive materials. We finally show that the same effects are at work in small tissues. Our work demonstrates that cellular organization and active mechanical response of a tissue is key to maintaining signal strength and leads to the emergence of elasticity, which means that signals are not dissipated like in a viscous system but can propagate over large distances.

opencc-zeroNov 2022View details →

opencc-by-4.0Apr 2022View details →

Substrate stiffness reduces particle uptake by epithelial cells and macrophages in a size-dependent manner through mechanoregulation

Cells continuously exert forces on their environment and respond to changes in mechanical forces by altering their behavior. Many pathologies such as cancer and fibrosis are hallmarked by dysregulation in the extracellular matrix, driving aberrant behavior through mechanotransduction pathways. We demonstrate that substrate stiffness can be used to regulate cellular endocytosis of particles in a size-dependent fashion. Culture of A549 epithelial cells and J774A.1 macrophages on polystyrene/glass (stiff) and polydimethylsiloxane (soft) substrates indicated that particle uptake is increased up to six times for A549 and two times for macrophages when cells are grown in softer environments. Furthermore, we altered surface characteristics through the attachment of submicron-sized particles as a method to locally engineer substrate stiffness and topography. To investigate the biomechanical changes which occurred within adherent epithelial cells, i.e. characterization of A549 cell spreading and focal adhesion maturation. Consequently, decreasing substrate rigidity and particle-based topography lead to a reduction of focal adhesion size. Moreover, expression levels of Yes-associated protein were found to correlate with the degree of particle endocytosis. A thorough appreciation of the mechanical cues may lead to improved solutions to optimize nanomedicine approaches for treatment of cancer and other diseases with abnormal mechanosignaling.

opencc-by-4.0Dec 2022View details →

Text-fig. 2. Light micrographs of Pinus spp. cuticles prepared with the modified, gentle bleaching procedure. a: Cuticle 1, Pinus sp. 1. Nearly the entire width of the leaf has been preserved. Five parallel rows of stomata are visible. b: Cuticle 1, close-up of (a). Two guard cells are visible around each stoma. c: Cuticle 1, close-up of eight stomata. Two guard cells and eight subsidiary cells are visible around each stoma. d: Cuticle 2, Pinus sp. 2. Some folding of the cuticle occurred during preparation, but many parallel rows of stomata on both sides of a thin, central midvein are evident. e: Cuticle 2, close-up of (d). Pairs of guard cells surround each stoma. f: Cuticle 2, close-up of (e). Subsidiary and epithelial cells can be observed around the stomata. in A Modified, Step-By-Step Procedure For The Gentle Bleaching Of Delicate Fossil Leaf Cuticles

Text-fig. 2. Light micrographs of Pinus spp. cuticles prepared with the modified, gentle bleaching procedure. a: Cuticle 1, Pinus sp. 1. Nearly the entire width of the leaf has been preserved. Five parallel rows of stomata are visible. b: Cuticle 1, close-up of (a). Two guard cells are visible around each stoma. c: Cuticle 1, close-up of eight stomata. Two guard cells and eight subsidiary cells are visible around each stoma. d: Cuticle 2, Pinus sp. 2. Some folding of the cuticle occurred during preparation, but many parallel rows of stomata on both sides of a thin, central midvein are evident. e: Cuticle 2, close-up of (d). Pairs of guard cells surround each stoma. f: Cuticle 2, close-up of (e). Subsidiary and epithelial cells can be observed around the stomata.