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Data and documentation from: Microclimate explains little variation in year-round decomposition across an Arctic tundra landscape
<p>The zip file contains data and code to reproduce the analysis in the submitted manuscript entitled <i>Microclimate explains little variation in year-round decomposition across an Arctic tundra landscape</i>. Please see the manuscript for further details on background, methodology, results and discussion.</p>
Temporal variation in spider trophic interactions is explained by the influence of weather on prey communities, web building and prey choice
<p>Materials and Methods</p> <p><em>Fieldwork</em><em> and sample processing</em></p> <p>Field collection and sample processing has been described previously by Cuff, Tercel, et al., (2022), but is briefly described in Supplementary Information 1. In short, money spiders (Araneae: Linyphiidae) and wolf spiders (Araneae: Lycosidae) were collected from occupied webs and the ground in barley fields between April and September 2018. Linyphiids occupying webs (n = 78) were prioritised for collection, but ground-active linyphiid and lycosid spiders were also collected. For each linyphiid taken from a web, the height of the web from the ground (mm) and its approximate dimensions were recorded, the latter calculated as approximate web area (mm<sup>2</sup>). To obtain data on local prey density, ground and crop stems were suction sampled using a ‘G-vac’ for approximately 30 seconds at each 4 m<sup>2</sup> quadrat from which spiders were collected. Extraction, amplification and sequencing of DNA, and bioinformatic analysis is described by Cuff, Tercel, et al. (2022) and Drake et al. (2022), and is also detailed in Supplementary Information 2. Amplification was carried out using two complementary PCR primer pairs: one targeting invertebrates generally, and one intended to exclude amplification of spider DNA to reduce the prevalence of ‘host’ reads in the data output (Cuff et al. 2023). Amplicons were sequenced via Illumina MiSeq V3 with 2x300 bp paired-end reads. The resultant sequencing read counts were converted to presence-absence data of each detected prey taxon in each individual spider. Given the prevalence of sequencing reads associated with each spider analysed and the impossibility of disentangling these from detections of intraspecific predation (i.e., cannibalism), all such reads were removed (Cuff et al. 2023), although intrageneric and intrafamilial predation were still detected.</p> <p> </p> <p><em>Weather data</em></p> <p>Weather data were taken from publicly available reports from the Cardiff Airport weather station (6.6 km from the study site) via “Wunderground” (Wunderground, 2020), to represent local weather conditions. This does not necessarily reflect smaller-scale effects (e.g., microclimate-scale; Bell, 2014; Holtzer et al., 1988), but the timescale of detection for dietary metabarcoding reduces the value of that resolution given that spiders may forage across multiple microclimates. We collated data from 1<sup>st</sup> January 2018 to 17<sup>th</sup> September 2018 (the last field collection). Weather data were also separately extracted for the week preceding each of the two 2017 collection dates (3<sup>rd</sup> to 9<sup>th</sup> August and 29<sup>th</sup> August to 4<sup>th</sup> September 2017). Specifically, daily average temperatures (°C), daily average dew point (°C), maximum daily wind speed (km h<sup>-1</sup>), daily sea level pressure (hPa) and day length (min; sunrise to sunset) were recorded. Precipitation data were downloaded via the UK Met Office Hadley Centre Observation Data (UK Met Office, 2020) as regional precipitation (mm) for South West England & Wales. Weather data were converted to mean values for seven days preceding the collection of spider samples to correspond with the longevity of DNA in the guts of spiders (Greenstone et al., 2014).</p> <p> </p> <p><em>Statistical Analysis</em></p> <p>All analyses were conducted in R v4.0.3 (R Core Team, 2020). To assess how weather affects spider trophic interactions over time, we analysed dietary changes across weather gradients using multivariate models. To identify whether this was likely to be driven by changes in prey abundance, we assessed the corresponding changes in the prey communities and then used null models to ascertain whether spiders were responding to prey abundance changes through prey choice. Given the dependence of linyphiid spiders on webs for foraging, we also compared web height and area over weather gradients to assess whether this may be a component of adaptive foraging. To assess the inter-annual consistency of prey choices in response to weather conditions, we also assessed whether prey preference data could be used to improve the predictive power of prey choice models. For this, we generated null models for 2017 data with prey abundance weighted by prey preferences estimated with the 2018 data. This allowed us to assess the consistency of prey choice under similar conditions, but also provides insight as to whether this framework can be used to predict predator responses to diverse prey communities under dynamic conditions. We detail the specific stages of this analytical framework in the below sections.</p> <p> </p> <p><em>Sampling completeness and diversity assessment</em></p> <p>To assess the diversity represented by the dietary analysis and the invertebrate community sampling, and the completeness of those datasets, coverage-based rarefaction and extrapolation were carried out, and Hill diversity calculated (Chao et al., 2014; Roswell, Dushoff, & Winfree, 2021). This was performed using the ‘iNEXT’ package with species represented by frequency-of-occurrence across samples (Chao et al., 2014; Hsieh et al., 2016; Figures S4 & S6).</p> <p> </p> <p><em>Relationships between weather, spider trophic interactions and prey community composition</em></p> <p>Prey species that occurred in only one spider individual were removed before further analyses to prevent outliers skewing the results. Spider trophic interactions were related to temporal and weather variables in multivariate generalized linear models (MGLMs) with a binomial error family (Wang, Naumann, Wright, & Warton, 2012). Trophic interactions were related to temporal variables and their pairwise interactions (including spider genus to account for any confounding effect), weather variables and their pairwise interactions, and weather variables and their interactions with spider genus and time (to account for any confounding effects) in three separate MGLMs. These variables were separated into different models (Temporal model, Weather interaction model and Confounding effects model) to improve model fit and reduce singularity. Invertebrate communities from suction sampling were related to temporal and weather variables in identically structured MGLMs (excluding the spider genus variable) with a Poisson error family.</p> <p>All MGLMs were fitted using the ‘manyglm’ function in the ‘mvabund’ package (Wang et al., 2012). ‘Temporal model’ independent variables were calendar day (<em>day</em>), mean day length in minutes for the preceding week (<em>day length</em>), spider genus (for dietary models only, to ascertain any effect of spider taxonomic differences on dietary differences over time and day lengths) and all two-way interactions between these variables. ‘Weather interaction model’ independent variables were mean temperature, precipitation, dewpoint, wind speed and pressure for the preceding week, and pairwise interactions between weather variables. ‘Confounding effects model’ independent variables were day (to investigate the interaction between time and weather), spider genus (for dietary models only, to ascertain any effect of spider taxonomic differences on dietary differences over time and day lengths), mean temperature, precipitation, dewpoint, wind speed and pressure for the preceding week, and two-way interactions of each weather variable with day and genus.</p> <p>Trophic interaction and community differences were visualised by non-metric multidimensional scaling (NMDS) using the ‘metaMDS’ function in the ‘vegan’ package (Oksanen et al., 2016) in two dimensions and 999 simulations, with Jaccard distance for spider diets and Bray-Curtis distance for invertebrate communities. For the dietary NMDS, outliers (n = 21; samples containing rare taxa) obscured variation on one axis and were thus removed to facilitate separation of samples and achieve minimum stress. For visualization of the effect of continuous variables against the NMDS, surf plots were created with scaled coloured contours using the ‘ordisurf’ function in the ‘ggplot’ package (Wickham, 2016).</p> <p> </p> <p><em>Relationships between web characteristics and weather variables</em></p> <p>Web area and height were compared against weather and temporal variables using a multivariate linear model (MLM) with the ‘manylm’ command in ‘mvabund’ (Wang et al., 2012). Log-transformed web area and height comprised the multivariate dependent variable, and day, spider genus, temperature, precipitation, dewpoint, wind, pressure and two-way interactions between each of these and day and genus comprised the independent variables.</p> <p> </p> <p><em>Variation in spider prey choice across weather conditions</em></p> <p>To separately represent spiders from different weather conditions in prey choice analyses, sample dates for every spider were clustered based on the mean weather conditions (temperature, precipitation, dewpoint, wind and pressure) of the week before collection (7 days, to align approximately with spider gut DNA half-life; Greenstone et al., 2014). Alongside data from 2018 (n = 24 collection dates), two sampling periods from 2017 were included in the clustering to ascertain similarity of weather conditions for additional inter-annual prey choice analyses described below. The clustering process is described in Supplementary Information 3. Five clusters were generated: High Pressure (HPR), Hot (HOT), Wet Low Dewpoint (WLD), Dry Windy (DWI), Wet Moderate Dewpoint (WMD), and 2017 (2017 sampling periods).</p> <p>Prey preferences of spiders in each of the weather clusters was analysed using network-based null models in the ‘econullnetr’ package (Vaughan et al., 2018) with the ‘generate_null_net’ command. Consumer nodes in this case represented spiders belonging to each of the weather clusters. Econullnetr generates null models based on prey abundance, represented here by suction sample data, to predict how consumers will forage if based on the abundance of resources alone. These null models are then compared against the observed interactions of consumers (i.e., interactions of spiders within each weather cluster with their prey) to ascertain the extent to which resource choice deviated from random (i.e., density dependence). The trophic network was visualised with the associated prey choice effect sizes using ‘igraph’ (Csardi & Nepusz, 2006) with a circular layout, and as a bipartite network using ‘ggnetwork’ (Briatte, 2021; Wickham, 2016). The normalised degree of each weather cluster node was generated using the ‘bipartite’ package (Dormann, Gruber, & Fruend, 2008) and compared against the normalised degree of the same node in the null network to determine whether spiders were more or less generalist than expected by random. Prior to the prey choice analysis, an hemipteran prey identified no further than order level through dietary analysis was removed due to the inability to pair it to any present prey taxa with certainty.</p> <p> </p> <p><em>Validating and predicting relationships between years</em></p> <p>To test how generalisable the results are and the extent to which weather drives prey preferences, we used a measure of prey preference (observed/expected values; observed interaction frequencies divided by interaction frequencies expected by null models) from the above prey choice analysis to assess whether we could more accurately predict observed trophic interactions under similar weather conditions for data from a linked study at the same location in 2017. These additional data represent a subset of the spider taxa analysed above (<em>Tenuiphantes tenuis</em> and <em>Erigone</em> spp.) collected using the same methods by the same researchers and in the same locality (Cuff, Drake, et al., 2021).</p> <p>The similarity in weather conditions between the 2017 study period and each of the five 2018 weather clusters was determined via NMDS of the weather data in two dimensions with Euclidean distance. Centroid coordinates for each 2018 weather cluster and the 2017 data were extracted and pairwise distances calculated between weather clusters:</p> <p> </p> <p>In order, the most proximate weather clusters to the 2017 weather data were HPR (mean Euclidean distance = 8.845), HOT (9.290), WMD (13.626), DWI (13.817) and WLD (18.682; Figure S3).</p> <p>To facilitate comparison between the two years, observed/expected values from the 2018 prey choice models were extracted separately for each of the weather clusters and scaled between 0.1 and 1. For this, 0.1 was used as a minimum since 0 would result in interactions being excluded altogether in the null models, and one as a maximum given the limits of econullnetr but also because this is a multiplier applied to the prey abundances, so greater values would skew prey abundances beyond realistic proportions. Scaling was achieved by the following equation:</p> <p> </p> <p>Missing values (e.g., prey that were absent in certain weather conditions) were represented as 1 to prevent transformation of their abundances in the null models; this treats prey for which data were absent naively, but could increase perceived preferences for them. The scaled values were used to weight the abundance of prey available to the spiders in the 2017 data using the weighting option in econullnetr, whereby values less than 1 proportionally reduce the probability of that taxon being predated in the null models. This effectively redistributes the 2017 relative prey abundance data according to the preference effect sizes generated for each of the 2018 weather clusters. If prey preferences are similar between the 2017 spiders and those from the weather cluster being used to weight the model, the composition of simulated diets should more closely resemble observed diets and fewer significant deviations from the null model should be found.</p> <p>Null models were generated as above (<em>Variation in spider prey choice across weather conditions</em>) but based on the prey availability and trophic interactions from 2017 samples. Three types of model were run: i) a conventional model based on observed prey abundances; ii) a model with prey abundances set to be equal across all prey taxa; and iii) observed prey abundances weighted by prey preferences determined for each of the weather clusters in the 2018 prey choice analysis. A separate model was run for each 2018 weather cluster with abundances weighted by the corresponding scaled observed/expected values. The unweighted conventional model was compared against weighted models to ascertain whether the prey preference weightings from 2018 improved the predictive power of the null models. To compare effect sizes between the unweighted and each other null model for each resource taxon, mean standardised effect size (SES) values were calculated from the paired ‘pre-harvest’ and ‘post-harvest’ data from each model, and paired <em>t</em>-tests were carried out with these between the unweighted and each weighted model. The SES values were plotted for each model and joined between taxa to visualise these paired differences using ‘ggplot’ (Wickham, 2016). Null model-predicted trophic interactions were generated via a modified ‘econullnetr’ function (generate_null_net_indiv) which produces outputs at the individual level to generate simulated diets for individual spiders to compare dietary composition between null model predictions and observed data. These models were run with 2300 simulations to represent 50 simulations per individual spider in the 2017 dataset (n = 46). Null diets were associated with sample IDs by aggregating the 50 simulations per sample and retaining a mean incidence of prey (i.e., mean occurrence across all 50 simulations). A visualisation of the per-sample differences in null model and observed data was generated via NMDS. Mean centroid coordinates for the observed 2017 data and the predicted diets of each model were extracted and the Euclidean distance between the observed data centroid and that of each model was calculated (as above for weather conditions).</p> <p> </p> <p><strong>Supplementary Information 1: Field collection and sample processing</strong></p> <p>Money spiders (Araneae: Linyphiidae) and wolf spiders (Araneae: Lycosidae) were visually located along transects in two adjacent barley fields at Burdons Farm, Wenvoe in South Wales (51°26'24.8"N, 3°16'17.9"W) and collected from occupied webs and the ground, between April and September 2018 (five visits per week of which spiders from 24 collection dates were used). Transects were randomly distributed across the entire field. Along these transects, 64 separate 4 m<sup>2</sup> quadrats, at least 10 m apart, were searched and all observed linyphiids and lycosids were collected. Spiders were placed in 100 % ethanol using an aspirator, regularly changing meshing to limit potential cross-contamination. Linyphiids occupying webs were prioritised for collection, but ground-active linyphiid spiders were also collected. For each spider taken from a web, the height of the web from the ground (mm) and its approximate dimensions were recorded, the latter calculated as approximate web area (mm<sup>2</sup>). Spiders were taken to Cardiff University, transferred to fresh ethanol, adults identified to species-level and juveniles to genus, and stored at -80 °C in 100 % ethanol until DNA extraction.</p> <p>To obtain data on local prey density, ground and crop stems were suction sampled using a ‘G-vac’ for approximately 30 seconds at each 4 m<sup>2</sup> quadrat (n = 64) from which spiders were collected. The collected material was emptied into a bag, any organisms immediately killed with ethyl-acetate and material frozen for storage before sorting into 70 % ethanol in the lab. All invertebrates were identified to family level to match the resolution of the least resolved of the metabarcoding-derived trophic interaction data, and due to difficulties associated with identification to finer taxonomic resolution for many taxa. Exceptions included springtails of the superfamily Sminthuroidea (Sminthuridae and Bourletiellidae were often indistinguishable following suction sampling and preservation due to the fine features necessary to distinguish them) which were left at super-family, mites (many of which were immature or in poor condition) which were identified to order level, and wasps of the superfamily Ichneumonoidea which were identified no further due to obscurity of wing venation due to damage.</p> <p> </p> <p><strong>Supplementary Information 2: Molecular analysis and bioinformatics</strong></p> <p><em>Extraction and high-throughput sequencing of spider gut DNA</em></p> <p>Given their prevalence in field collections, dietary analysis was carried out for the linyphiid genera <em>Erigone</em>, <em>Tenuiphantes</em>, <em>Bathyphantes</em> and <em>Microlinyphia </em>(Araneae: Linyphiidae), and the Lycosidae genus <em>Pardosa</em>. Spiders were transferred to and washed in fresh 100 % ethanol to reduce external contaminants prior to identification via morphological key (Roberts, 1993). Abdomens were removed from spiders and again transferred to and washed in fresh 100 % ethanol. DNA was extracted from the abdomens via Qiagen TissueLyser II and DNeasy Blood & Tissue Kit (Qiagen) as per the manufacturer protocol, but with an extended lysis time of 12 hours to account for the complex and branched gut system in spider abdomens (Krehenwinkel et al., 2017).</p> <p>For amplification of DNA, two primer pairs were used. BerenF-LuthienR (Cuff et al., 2021) amplified a broad range of invertebrates including spiders, and TelperionF-LaureR (Cuff et al., 2022), amplified a range of invertebrates but fewer spiders. Primers were labelled with unique 10 bp molecular identifier tags (MID-tags) so that each individual had a unique pairing of forward and reverse tags for identification of each spider post-sequencing. PCR reactions of 25 µl contained 12.5 µl Qiagen PCR Multiplex kit, 0.2 µmol (2.5 µl of 2 µM) of each primer and 5 µl template DNA. Reactions were carried out in the same thermocycler, optimised via temperature gradient, with an initial 15 minutes at 95 °C, 35 cycles of 95 °C for 30 seconds, the primer-specific annealing temperature for 90 seconds and 72 °C for 90 seconds, respectively, followed by a final extension at 72 °C for 10 minutes. BerenF-LuthienR and TelperionF-LaureR used annealing temperatures of 52 °C and 42 °C, respectively.</p> <p>Within each PCR 96-well plate, 12 negative controls (extraction and PCR), 2 blank controls and 2 positive controls were included (i.e. 80 samples per plate), based on Taberlet <em>et al. </em>(2018). Positive controls were mixtures of invertebrate DNA comprised of non-native Asiatic species in four different proportions and blanks were empty wells within each plate to identify tag-jumping into unused MID-tag combinations. PCR negative controls were DNase-free water treated identically to DNA samples. A negative control was present for each MID-tag to identify any contamination of primers. All PCR products were visualised in a 2 % agarose gel with SYBRSafe (Thermo Fisher Scientific, Paisley, UK) and placed in categories based on their relative brightness. The concentration of these brightness categories was quantified via Qubit dsDNA High-sensitivity Assay Kits (Thermo Fisher Scientific, Waltham, MA, USA) with at least three representatives of each category per plate. The PCR products were then proportionally pooled according to these concentrations. Each pool was cleaned via SPRIselect beads (Beckman Coulter, Brea, USA), with a left-side size selection using a 1:1 ratio (retaining ~300-1000 bp fragments). The concentration of the pooled DNA was then determined via Qubit dsDNA High-sensitivity Assay Kits and pooled together into one library per primer pair. Library preparation for Illumina sequencing was carried out on the cleaned libraries via NEXTflex Rapid DNA-Seq Kit (Bioo Scientific, Austin, USA) and samples were sequenced on an Illumina MiSeq via a V3 chip with 300-bp paired-end reads (expected capacity ≤25,000,000 reads).</p> <p><em>Bioinformatic analysis</em></p> <p>Bioinformatic analysis followed Drake et al., (2022). The Illumina run generated 11,165,405 and 10,959,010 reads for BerenF-LuthienR and TelperionF-LaureR, respectively, which were quality-checked and paired via FastP (Chen et al., 2018) to retain only sequences of at least 200 bp with a quality threshold of 33, resulting in 10,561,874 and 9,355,112 paired reads. The paired reads were demultiplexed and assigned to their respective spider sample according to their MID-tags via the “trim.seqs” command in Mothur v1.39.5 (Schloss et al., 2009), leaving 7,854,610 and 7,437,929 reads with exact matches to the primer and MID-tags.</p> <p>Replicates were removed, and denoising and clustering to zero-radius operational taxonomic units (ZOTUs; clustered without % identity to avoid multiple species represented within a single operational taxonomic unit (OTU)) completed via Unoise3 in Usearch11 (Edgar, 2010). The resultant sequences were assigned a taxonomic identity from GenBank via BLASTn v2.7.1 (Camacho et al., 2009) using a 97 % identity threshold (Alberdi et al., 2017). The BLAST output was analysed in MEGAN v6.15.2 (Huson et al., 2016). Where the top BLAST hit, determined by lowest e-value, was resolved at a higher taxonomic level than species-level, the results were checked; where possibly erroneous entries were preventing species-level assignment (e.g., poorly resolved identifications on GenBank), finer resolution was assigned based on the next-closest match. Where ZOTUs were assigned the same taxon, these were aggregated.</p> <p>Data clean-up used the optimal minimum sequence copy thresholds identified by Drake et al. (2022). The maximum value for a ZOTU present in blank or negative controls was identified and subtracted from all read counts for that ZOTU to remove background contaminants. Simultaneously, known lab contaminants (e.g., German cockroach <em>Blattella germanica</em>), artefacts and errors of the sequencing process, unexpected reads in positive controls and positive control taxon reads in dietary samples were identified. These were calculated as a percentage of their respective sample’s read count and any read counts lower than the highest of these percentages for their respective sample were removed to eliminate additional instances of contamination. These thresholds were defined as 0.38 % and 0.39 % for BerenF-LuthienR and TelperionF-LaureR, respectively. The data from the two libraries (i.e., from each primer pair) were then aggregated together by sample and aggregated again by taxon. Non-target taxa (e.g., fungi) and instances in which predator DNA was amplified (i.e., ZOTUs with high read counts matching the individual’s morphological identity) were removed. All remaining read counts were converted to presence-absence.</p> <p> </p> <p><strong>Supplementary Information 3: Cluster analysis</strong></p> <p>Prior to clustering, weather variables were scaled by subtracting the mean and dividing by the standard deviation. A Euclidean distance matrix was calculated using the ‘dist’ function, and this scaled distance matrix was hierarchically clustered using the ‘hclust’ function. Optimal clustering solutions were determined by comparison of Dunn’s index between methods and <em>k</em> values; this was calculated using the ‘dunn’ function in the “clValid” package (Brock et al., 2008) for each cluster <em>k</em> value above five until the Dunn index decreased. The <em>k</em> value after which Dunn’s index decreased was deemed the optimal solution for each clustering method. Clustering methods based on ‘average’, ‘complete’, ‘single’, ‘median’, ‘centroid’ and ‘mcquitty’ linkages were compared, and the ‘complete’ method selected for subsequent analysis as it resulted in the smallest number of clusters (6; thus, the most efficient simplification of the data; Figure S1). Different clustering methods altered the composition of some clusters, but most sampling dates showed consistent clustering between methods.</p> <p>A heatmap dendrogram was produced using the ‘heatmap.2’ function in the ‘gplots’ package (Warnes et al., 2020), with cluster colours assigned with the ‘Accent’ palette of ‘RColorBrewer’ (Neuwirth, 2014) and relative weather value colour scaling generated using the ‘viridis’ package (Garnier 2018; Figure S2). Weather clusters were named according to unique characteristics relative to the other clusters. These names comprise: High Pressure (HPR; days 142, 253, 256, 250, 173), Hot (HOT; days 162, 204, 205, 208, 201, 187, 198, 197, 183, 184, 194, 190 and 191), Wet Low Dewpoint (WLD; day 121), Dry Windy (DWI; days 131, 169 and 170), Wet Moderate Dewpoint (WMD; days 149 and 152), and 2017 (pre- and post-harvest 2017 sampling periods).</p> <p> </p> <p> </p> <p><strong>References</strong></p> <p>Alberdi, A., Aizpurua, O., Gilbert, M. T. P., & Bohmann, K. (2017). Scrutinizing key steps for reliable metabarcoding of environmental samples. <em>Methods in Ecology and Evolution</em>, <em>9</em>(1), 1–14. https://doi.org/10.1111/2041-210X.12849</p> <p>Brock, G., Pihur, V., Datta, S., & Datta, S. (2008). clValid: an R package for cluster validation. <em>Journal of Statistical Software</em>, <em>25</em>(4), 1–22.</p> <p>Camacho, C., Coulouris, G., Avagyan, V., Ma, N., Papadopoulos, J., Bealer, K., & Madden, T. L. (2009). BLAST+: architecture and applications. <em>BMC Bioinformatics</em>, <em>10</em>, 1–9. https://doi.org/10.1186/1471-2105-10-421</p> <p>Chen, S., Zhou, Y., Chen, Y., & Gu, J. (2018). Fastp: An ultra-fast all-in-one FASTQ preprocessor. <em>Bioinformatics</em>, <em>34</em>(17), i884–i890. https://doi.org/10.1093/bioinformatics/bty560</p> <p>Cuff, J. P., Drake, L. E., Tercel, M. P. T. G., Stockdale, J. E., Orozco-terWengel, P., Bell, J. R., Vaughan, I. P., Müller, C. T., & Symondson, W. O. C. (2021). Money spider dietary choice in pre- and post-harvest cereal crops using metabarcoding. <em>Ecological Entomology</em>, <em>46</em>(2), 249–261.</p> <p>Cuff, J. P., Tercel, M. P. T. G., Drake, L. E., Vaughan, I. P., Bell, J. R., Orozco-terWengel, P., Müller, C. T., & Symondson, W. O. C. (2022). Density-independent prey choice, taxonomy, life history and web characteristics determine the diet and biocontrol potential of spiders (Linyphiidae and Lycosidae) in cereal crops. <em>Environmental DNA</em>, <em>4</em>(3), 549–564.</p> <p>Drake, L. E., Cuff, J. P., Young, R. E., Marchbank, A., Chadwick, E. A., & Symondson, W. O. C. (2022). An assessment of minimum sequence copy thresholds for identifying and reducing the prevalence of artefacts in dietary metabarcoding data. <em>Methods in Ecology and Evolution</em>, <em>13</em>(3), 694–710.</p> <p>Edgar, R. C. (2010). Search and clustering orders of magnitude faster than BLAST. <em>Bioinformatics</em>, <em>26</em>(19), 2460–2461. https://doi.org/10.1093/bioinformatics/btq461</p> <p>Garnier, S. (2018). <em>viridis: default color maps from ‘matplotlib’</em> (0.5.1). https://cran.r-project.org/package=viridis</p> <p>Huson, D. H., Beier, S., Flade, I., Górska, A., El-Hadidi, M., Mitra, S., Ruscheweyh, H. J., & Tappu, R. (2016). MEGAN Community Edition - interactive exploration and analysis of large-scale microbiome sequencing data. <em>PLoS Computational Biology</em>, <em>12</em>(6), 1–12. https://doi.org/10.1371/journal.pcbi.1004957</p> <p>Krehenwinkel, H., Kennedy, S., Pekár, S., & Gillespie, R. G. (2017). A cost-efficient and simple protocol to enrich prey DNA from extractions of predatory arthropods for large-scale gut content analysis by Illumina sequencing. <em>Methods in Ecology and Evolution</em>, <em>8</em>, 126–134. https://doi.org/10.1111/2041-210X.12647</p> <p>Neuwirth, E. (2014). <em>RColorBrewer: ColorBrewer palettes</em> (1.1-2). https://cran.r-project.org/package=RColorBrewer</p> <p>Roberts, M. J. (1993). <em>The Spiders of Great Britain and Ireland (Compact Edition)</em> (3rd ed.). Harley Books.</p> <p>Schloss, P. D., Westcott, S. L., Ryabin, T., Hall, J. R., Hartmann, M., Hollister, E. B., Lesniewski, R. A., Oakley, B. B., Parks, D. H., Robinson, C. J., Sahl, J. W., Stres, B., Thallinger, G. G., Van Horn, D. J., & Weber, C. F. (2009). Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. <em>Applied and Environmental Microbiology</em>, <em>75</em>(23), 7537–7541. https://doi.org/10.1128/AEM.01541-09</p> <p>Taberlet, P., Bonin, A., Zinger, L., & Coissac, E. (2018). <em>Environmental DNA</em>. Oxford University Press.</p> <p>Warnes, G. R., Bolker, B., Bonebakker, L., Gentleman, R., Huber, W., Liaw, A., Lumley, T., Maechler, M., Magnusson, A., Moeller, S., Schwartz, M., & Venables, B. (2020). <em>gplots: Various R programming tools for plotting data</em> (R package version 3.1.0). https://cran.r-project.org/package=gplots</p>
Data from: Biotic interactions help explain variation in elevational range limits of birds among Bornean mountains
Aim <p>Physiological tolerances and biotic interactions along habitat gradients are thought to influence species occurrence. Distributional differences caused by such forces are particularly noticeable on tropical mountains, where high species turnover along elevational gradients occurs over relatively short distances and elevational distributions of particular species can shift among mountains. Such shifts are interpreted as evidence of the importance of spatial variation in interspecific competition and habitat or climatic gradients. To assess the relative importance of competition and compression of habitat and climatic zones in setting range limits, we examined differences in elevational ranges of forest bird species among four Bornean mountains with distinct features.</p> Location <p>Bornean mountains Kinabalu, Mulu, Pueh and Topap Oso.</p> Taxon <p>Rain forest bird communities along elevational gradients.</p> Methods <p>We surveyed the elevational ranges of rain forest birds on four mountains in Borneo to test which environmental variables—habitat zone compression or presence of likely competitors—best predicted differences in elevational ranges of species among mountains. For this purpose, we used two complementary tests: a comparison of elevational range limits between pairs of mountains, and linear mixed models with naïve occupancy as the response variable.</p> Results <p>We found that lowland species occur higher in elevation on two small mountains compared to Mt. Mulu. This result is inconsistent with the expectation that distributions of habitats are elevationally compressed on small mountains, but is consistent with the hypothesis that a reduction in competition (likely diffuse) on short mountains, which largely lack montane specialist species, allows lowland species to occur higher in elevation. The relative influence of competition changes with elevation, and the correlation between lower range limits of montane species and the distribution of their competitors was weaker than in lowland species.</p> Main conclusions <p>These findings provide support for the importance of biotic interactions in setting elevational range limits of tropical bird species, although abiotic gradients explain the majority of distribution patterns. Thus, models predicting range shifts under climate change scenarios must include not only climatic variables, as is currently most common, but also information on potentially resulting changes in species interactions, especially for lowland species.</p>
Data and script for: A marker of biological age explains individual variation in the strength of the adult stress response
<p>Data and script for: A marker of biological age explains individual variation in the strength of the adult stress response.</p> <p>Comprises 2 CSV data files and one R script.</p>
Data from: Environmental variation associated with topography explains butterfly diversity along a tropical elevation gradient
<p>Few studies have evaluated the role of topography on the diversity patterns of biological communities along elevation gradients. We evaluated the influence of microclimate and vegetation structure associated with topographic variation on the richness and composition of species of different families of butterflies on a mountain located in a dry enclave (Chicamocha River Canyon) in the northern Andes, Colombia. We captured butterflies over four months at 18 elevations (300 to 1500 m a.s.l.) in two topographic positions (riverbed and hillslope) using an entomological net and traps baited with fermented fruit. In general, butterfly richness increased with elevation in both topographic positions. However, the richness-elevation relationship changed with butterfly family. The riverbed and hillslope sites host different assemblages of butterflies, and this pattern that was consistent for most families. In the riverbed, two sets of species are recognized along the elevation gradient (one below 700 m a.s.l. and the other above 1000 m a.s.l.), mainly owing to species replacement. On the hillslopes there was no clear pattern of grouping associated with elevation. Microclimate differences between the riverbed and hillslope sites along the elevation gradient were related to the vegetation structure and explained the variation in butterfly species composition. Our results highlight the role of topography not only by explaining the response of species richness and composition to environmental variation determined by elevation, but also as a factor that must be considered in the planning and management of biodiversity conservation in the mountains.</p>
Dataset: Environmental conditions and male quality traits simultaneously explain variation of multiple colour signals in male lizards
<p>Dataset and R code associated with the following publication:</p> <p>Badiane et al. (2022), Environmental conditions and male quality traits simultaneously explain variation of multiple colour signals in male lizards. Journal of Animal Ecology, in press</p> <p>This dataset includes the following files:</p> <p>- An excel file containing the reflectance spectra of all individuals from all the study populations</p> <p>- An excel file containing the variables collected at the individual and population levels</p> <p>- Two R scripts corresponding to the analyses performed in the publication</p>
FIGURE 1 in Testing spatial and environmental factors to explain body shape variation in the widespread Central American Blackbelt cichlid Vieja maculicauda (Teleostei: Cichlidae)
FIGURE 1 | Points representing geographic location for the lots of Vieja maculicauda used in the current study. Straight black lines represent the approximate location of geological block divisions. Purple shading represents a modified version of IUCN redlist data for the distribution of this species (Lyons, 2019).
FIGURE 4 in Testing spatial and environmental factors to explain body shape variation in the widespread Central American Blackbelt cichlid Vieja maculicauda (Teleostei: Cichlidae)
FIGURE 4 | Canonical variate analysis and shape changes along both axes. Shape change has been magnified by two for increased visualization.
FIGURE 3 in Testing spatial and environmental factors to explain body shape variation in the widespread Central American Blackbelt cichlid Vieja maculicauda (Teleostei: Cichlidae)
FIGURE 3 | Principal component analysis of size-corrected shape and deformation grids along each axis.
FIGURE 2 in Testing spatial and environmental factors to explain body shape variation in the widespread Central American Blackbelt cichlid Vieja maculicauda (Teleostei: Cichlidae)
FIGURE 2 | Landmarks (in blue) and semilandmarks (in red) as placed on each specimen. Landmark positions are described on Tab. S1.
Supplementary material to "Exogenous corticosterone and melanin-based coloration explain variation in juvenile dispersal behaviour in the barn owl (Tyto alba)"
<p><strong>Abstract</strong></p> <p>Natal dispersal affects many processes such as population dynamics. So far, most studies have examined the intrinsic and extrinsic factors that determine the distance between the place of birth and of first breeding. In contrast, few researchers followed the first steps of dispersal soon after fledging. To study this gap, we radio-tracked 95 barn owl nestlings (<em>Tyto alba</em>) to locate their diurnal roost sites from the fledging stage until December. This was used to test whether the age of nest departure, post-fledging movements and dispersal distance were related to melanin-based coloration, which is correlated to fitness-related traits, as well as to corticosterone, a hormone that mediates a number of life history trade-offs and the physiological and behavioural responses to stressful situations. We found that the artificial administration of corticosterone delayed the age when juveniles left their parental home-range in females but not in males. During the first few months after fledging, longer dispersal distances were reached by females compared to males, by individuals marked with larger black feather spots compared to individuals with smaller spots, by larger individuals and by those experimentally treated with corticosterone. We conclude that the onset and magnitude of dispersal is sensitive to the stress hormone corticosterone, melanin-based coloration and body size. </p>
Data for: The interactive effects of soil fertility and tree mycorrhizal association explain spatial variation of diversity-biomass relationships in a subtropical forest
<p><span>Observed biodiversity-ecosystem function (BEF) relationships are highly variable, particularly in natural forests. However, our understanding of the factors that generate these often contradictory patterns, especially the role of different mycorrhizal associations, is still limited. By relating tree species richness and aboveground biomass (AGB) in a fully-mapped 24-ha subtropical forest dynamics plot, we evaluated the impacts of soil fertility and tree mycorrhizal type in mediating BEF relationships at multiple spatial scales. Our results demonstrate a highly positive total richness effect on AGB for arbuscular mycorrhizal (AM) trees but a negative effect on AGB for ectomycorrhizal (EcM) trees, and their relationships were highly spatial scale dependent. However, the observed BEF relationships turned into positive at small spatial scales (i.e., 10 m and 20 m) after controlling for other confounding factors (i.e., topography, soil fertility, and AM proportion). In addition, we found significant interactions between soil fertility and species richness on AGB. Specifically, the positive effect of total species richness on AGB for major mycorrhizal types gradually weakened with increasing soil fertility, while the positive effect of EcM species richness on AM AGB gradually enhanced at small spatial scales, suggesting the observed diversity effects can be largely attributed to resource niche complementarity and the role of EcM fungi.</span></p> <p><span>Synthesis. We conclude that the variable BEF relationships among forest communities could be explained by spatial variation in abiotic environments and community mycorrhizal composition because different types of symbionts perform different nutrient uptake strategies and ability in protection from antagonists. Our findings provide novel insights into the understanding of the variation in the shape of BEF relationships in natural forests, which is critical for forest management, conservation, and restoration in a changing world.</span></p>
Data for: The interactive effects of soil fertility and tree mycorrhizal association explain spatial variation of diversity-biomass relationships in a subtropical forest
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Differential responses to weather and land-cover conditions explain spatial variation in winter abundance trends in a migratory bird of conservation concern
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Data from: Biotic interactions help explain variation in elevational range limits of birds among Bornean mountains
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Habitat availability is insufficient to explain regional variations in white stork breeding habitat preference
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Data from: Variation in activity rates may explain sex-specific dorsal color patterns in Habronattus jumping spiders
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Data from: Environmental variation associated with topography explains butterfly diversity along a tropical elevation gradient
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Explaining global variation in the latitudinal diversity gradient: Meta-analysis confirms known patterns and uncovers new one
This dataset is also available on the Dryad Digital Repository (link: https://doi.org/10.5061/dryad.rg5rd). The code is also available on GitHub (link: https://github.com/nlkinlock/LDGmeta-analysis). This dataset was created to explore patterns in biodiversity across latitude. The pattern of increasing biological diversity from high latitudes to the equator [latitudinal diversity gradient (LDG)] has been recognized for greater than 200 years. Empirical studies have documented this pattern across many different organisms and locations. In order to quantify the evidence for the global LDG and the associated spatial, taxonomic and environmental factors, a systematic review, followed by a meta-analysis of the resulting dataset, were carried out. This dataset contains a large number of individual LDGs that have been published in the 14 years since Hillebrand's ground‐breaking meta‐analysis of the LDG.
Datasets - Evolutionary history, not ecogeographic rules, explains size variation of tropical insects along elevational gradients
<p>One of the best-known biogeographic rules for ectotherms is the temperature-size rule, which asserts that ectotherms produce smaller adults at warmer temperatures. Although this is often true, it has become clear that there is no single process behind the pattern and many exceptions to the rule. To disentangle such complex temperature-size relationships, individual clades must be examined at ecological and evolutionary scales.</p> <p>We examined temperature-size relationships for 2106 individuals from 64 populations and 40 species of <i>Cephaloleia</i> rolled-leaf beetles (Chrysomelidae; Cassidinae)<b> </b>occurring along two tropical elevational gradientss: Barva and the Talamanca Cordillera in Costa Rica, Central America. We tested whether the temperature-size rule applied to interspecific elevational assemblages, intraspecific elevational populations, or different rearing temperatures for individual populations.</p> <p>At the interspecific scale, evolutionary history, rather than elevation, explains body size. At the intraspecific scale, only one of seven species followed the temperature-size rule across elevations. When larvae were reared at different temperatures, only one of five populations followed the temperature-size rule. Most populations grew to a fixed size regardless of temperature.</p> <p>Size in <i>Cephaloleia</i> beetles is constrained by their evolutionary history and responds to factors that rarely correlate with temperature. As temperature increases, ectotherms will not universally shrink, but determining if and why their size will change will require further investigation.</p> <p>Here we provide the following datasets used to determine effects of temperature on insect body size at population and community levels: <i>Supplement S1.</i> Body length for 2106 individuals representing 64 populations from 40 species of <i>Cephaloleia </i>rolled-leaf beetle along the Barva and Talamanca elevational gradients, Costa Rica<i>. Supplement S2.</i> Intraspecific differences in length for 798 individuals from seven <i>Cephaloleia </i>species present at multiple life zone<i>.</i><i> Supplement S3.</i> Effects of developmental temperatures on <i>Cephaloleia </i>adult size in 968 individuals from four species and five populations reared in the laboratory at temperatures between 10 and 35 °C.</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.