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opencc-by-4.0Nov 2020View details →

Elongation of very long chain fatty acids protein 7 (ELOVL7); A Target Enabling Package

The long-chain fatty acid elongases (ELOVL) catalyse the first rate-limiting step in the two carbon elongation of the acyl chains of fatty acids (FAs) greater than 12 carbons in length. Defects in these ELOVL elongases cause severe genetic diseases, such as Stargardt disease-3 and several ataxias, and knockout studies suggest roles in insulin resistance and hepatic steatosis. This TEP provides the first structural information for this family of enzymes which, coupled with mutagenesis and biophysical studies, demonstrates how substrates and products bind within the active site.

opencc-by-4.0Nov 2021View details →

Bioactive fatty-acid derivated ovalicin from Pseudallescheria boydii

Original data for the mMolecular network with t-SNE representation of crude extracts of P. boydii. SNB-CN71, -CN73, -CN81 and -CN85

opencc-by-4.0Dec 2021View details →

Dataset of plasma non-esterified fatty acid concentrations response of a suckling cow exposed to a feed restriction

The detaset describes the response of a suckler cow in terms of plasma non-esterified fatty-acids (NEFA) concentrations, that was exposed to a feed restriction that consisted in the reduction of net energy requirements by 50%. The dataset has two columns, one for time (t) in days (d) and another column for plasma NEFA concentrations (g·L-1). The feed restriction started at t = 1 d and lasted untill t = 4 d. Negative values for t represent the pre-challenge period.

opencc-by-4.0Sep 2022View details →

Data Set for_Integrating torrefaction of pulp industry sludge with anaerobic digestion to produce biomethane and volatile fatty acids: An example of industrial symbiosis for circular bioeconomy

Industrial symbiosis, which allows the sharing of resources between different industries, could help to improve the overall feasibility of bio-based chemicals production. In that regard, this study focused on integrating the torrefaction of pulp industry sludge with anaerobic digestion. More specifically, anaerobic digestion (AD) of pulp sludge-derived torrefaction condensate (TC) was studied to evaluate the biomethane and volatile fatty acid (VFA) potential. The torrefaction condensate produced at 275 and 300 °C was used in AD. The volatile solid content (VS) was 6.69 and 9.01% for the condensate produced at 275 and 300 °C, respectively. The organic fraction of TC mainly contained acetic acid, 2-furanmethanol, and syringol. The methane yield was in the range of 481–772 mL/g VS for the mesophilic and 401–746 mL/g VS for the thermophilic process, respectively. The VFA yield was in the range of 1.1 to 3.4 g/g VS for mesophilic and from 1.5 to 4.7 g/g VS in thermophilic conditions, when methanogenesis was inhibited. Finally, pulp sludge TC is a feasible feedstock to produce platform chemicals like VFA. However, at higher substrate loading, signs of process inhibition were observed because of the relatively increasing concentration of microbial inhibitors

opencc-by-sa-4.0Jan 2018View details →

Characterization of the polyspecific transferase of murine type I fatty acid synthase (FAS) and implications for polyketide synthase (PKS) engineering

Characterization of the polyspecific transferase of murine type I fatty acid synthase (FAS) and implications for polyketide synthase (PKS) engineering <a href="https://dx.doi.org/10.1021/acschembio.7b00718">https://dx.doi.org/10.1021/acschembio.7b00718</a> Abstract Fatty acid synthases (FASs) and polyketide synthases (PKSs) condense acyl compounds to fatty acids and polyketides, respectively. Both, FASs and PKSs, harbor acyltransferases (ATs), which select substrates for condensation by β-ketoacyl synthases (KSs). Here, we present the structural and functional characterization of the polyspecific malonyl/acetyltransferase (MAT) of murine FAS. We assign kinetic constants for the transacylation of the native substrates, acetyl- and malonyl-CoA, and demonstrate the promiscuity of FAS to accept structurally and chemically diverse CoA-esters. X-ray structural data of the KS-MAT didomain in a malonyl-loaded state suggests a MAT-specific role of an active site arginine in transacylation. Owing to its enzymatic properties and its accessibility as a separate domain, MAT of murine FAS may serve as versatile tool for engineering PKSs to provide custom-tailored access to new polyketides that can be applied in antibiotic and antineoplastic therapy. Raw dataset for protein databank accession code (PDB) 5my0 <a href="http://dx.doi.org/10.2210/pdb5my0/pdb">http://dx.doi.org/10.2210/pdb5my0/pdb</a>

opencc-by-4.0Sep 2019View details →

Homology modelling, molecular docking and molecular dynamics simulations of wild type and mutant human CYP2J2 with three polyunsaturated fatty acids

This is the "parent" repository for the Data Note : "Molecular dynamics simulations of the interaction of wild type and mutant human CYP2J2 with polyunsaturated fatty acids" by Abelak, Bishop-Bailey and Nobeli. It contains a document (Abelak_etal_Methods.pdf) describing the methods used to produce the data here and the data in all repositories supplementing it. It also contains a shell script (create_sim4_repeats.sh) that is typical of those used to set up the molecular dynamics simulations in the repositories supplementing this one. Finally, it contains the results of the homology modelling and docking simulations that formed the starting points for the molecular dynamics simulations in this study. Description of files in this dataset: C2J2_min3_mod_noH.pdb : Homology model of the wild type CYP2J2 built from an alignment of templates with PDB ids: 1SUO, 2P85, 3EBS and 1Z10. docking_wild_type_C2J2.zip : Nine docked poses of arachidonic acid docked to the homology model of the wild type CYP2J2. Details of how this data was produced is available in the Abelak_etal_Methods.docx document.

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Fatty acid data among habitats and between seasons in Everglades National Park, Florida, USA, 2018-2019

Fatty acid profiles to represent the aquatic food web of the freshwater habitats from wet and dry seasons of the Everglades, Florida, USA. This dataset provides fatty acid profiles for a range of food-web members from basal resources (vascular plants, benthic flocculent matter, periphyton), aquatic invertebrates, amphibians, and fishes (including non-native and invasive species). Each sample is a composite of six individuals on average. Samples were collected from multiple habitats across sites in Shark River Slough and Taylor Slough in the wet (2018) and dry (2019) seasons. In some cases, in order to have enough tissue to make a composite sample, the composite was made at a higher level then at a habitat within a site. For example, some composites are from the same slough-season-habitat and combined from multiple sites. The goal was to capture as much of the aquatic food web in the wet and dry seasons as possible to build fatty-acid derived food-web snap shots, not necessarily to quantify variation at various levels of different factors (e.g., habitat and site). However, common species are relatively well replicated among factor levels. Data collection is complete.

openCC (other)Dec 2025View details →

Data_Figure 2_Impact of 17β‑HSD12, the 3‑ketoacyl‑CoA reductase of long‑chain fatty acid synthesis, on breast cancer cell proliferation and migration

Data of figure 2 from Impact of 17β‑HSD12, the 3‑ketoacyl‑CoA reductase of long‑chain fatty acid synthesis, on breast cancer cell proliferation and migration Dataset (doi: 10.1007/s00018-019-03227-w) contains the original figure as TIF-format (10.1007_s00018-019-03227-w_CMLS_Fig2). Corresponding raw data obtained from a) Migration potential as four files in CSV format (31003A-179400_ date_examiner_17BHSD12_16_1_1-4. b) mRNA content analyzed by RT-PCR provided as ten files in CSV format (31003A-179400_date_examiner_17BHSD12_1_1-2_1-6) and proliferation investigation on xCELLigence provided as six files in CSV format (31003A-179400_date_examiner_17BHSD12_9_2_1-6). All further experiment related information and subsequent data analysis provided as four meta-data-files (31003A-179400_ date_examiner_17BHSD12_16/1/9_dataset_M_1) as TXT format.

Data_Figure 6_Impact of 17β‑HSD12, the 3‑ketoacyl‑CoA reductase of long‑chain fatty acid synthesis, on breast cancer cell proliferation and migration

Data of figure 6 from Impact of 17β‑HSD12, the 3‑ketoacyl‑CoA reductase of long‑chain fatty acid synthesis, on breast cancer cell proliferation and migration Dataset (doi: 10.1007/s00018-019-03227-w) contains the original figure as TIF-format (10.1194_jlr.M092908_Fig. 6). Corresponding raw data obtained from a1/2) cellomics HTC array scan analysis provided as six files in CSV format (31003A-179400_Date_examiner_17BHSD12_8_6_1-6), b1/2) oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) provided as 10 files in CSV format (31003A-179400_20190521_MT_17BHSD12_10_3-4_1-5); c 1/2 ), cellomics HTC array scan analysis provided as 12 files in CSV format (31003A-179400_Date_examiner_17BHSD12_8_7-8_1-8). d) Western blot and densitometry provided as eight files in CSV format (31003A-179400_Date_examiner_17BHSD12_2_3-4_1-5). All further experiment related information protocols and subsequent data analysis provided as meta-data-files (31003A-179400_date_examiner_17BHSD12_8/10/2_dataset_M_1) as TXT format and (31003A-179400_date_examiner_17BHSD12_2_dataset_M_2-3) as PNG format.

Data_Figure 7_Impact of 17β‑HSD12, the 3‑ketoacyl‑CoA reductase of long‑chain fatty acid synthesis, on breast cancer cell proliferation and migration

Data of figure 7 from Impact of 17β‑HSD12, the 3‑ketoacyl‑CoA reductase of long‑chain fatty acid synthesis, on breast cancer cell proliferation and migration Dataset (doi: 10.1007/s00018-019-03227-w) contains the original figure as TIF-format (10.1194_jlr.M092908_Fig. 7). Corresponding raw data obtained from a1/2) Western blot and densitometry provided as eight files in CSV format (31003A-179400_date_examiner_17BHSD12_2_5-6_1-6); b) mRNA content analyzed by RT-PCR provided as four files in CSV format (31003A-179400_date_examiner_17BHSD12_1_6_1-4); c 1/2) cellomics HTC array scan analysis provided as 11 files in CSV format (31003A-179400_Date_examiner_17BHSD12_8_9-10_1-6); d1/2); Western blot and densitometry provided as eight files in CSV format (31003A-179400_date_examiner_17BHSD12_2_7-8_1-6). All further experiment related information protocols and subsequent data analysis provided as meta-data-files (31003A-179400_date_examiner_17BHSD12_2/1/8_dataset_M_1) as TXT format and (31003A-179400_date_examiner_17BHSD12_2_dataset_M_2-3) as PNG format.

Data_Figure 5_Impact of 17β‑HSD12, the 3‑ketoacyl‑CoA reductase of long‑chain fatty acid synthesis, on breast cancer cell proliferation and migration

Data of figure 5 from Impact of 17β‑HSD12, the 3‑ketoacyl‑CoA reductase of long‑chain fatty acid synthesis, on breast cancer cell proliferation and migration Dataset (doi: 10.1007/s00018-019-03227-w) contains the original figure as TIF-format (10.1194_jlr.M092908_Fig. 5). Corresponding raw data obtained from oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) provided as 10 files in CSV format (31003A-179400_20190521_MT_17BHSD12_10_1-2_1-5). All further experiment related information and subsequent data analysis provided as two meta-data-file: (31003A-179400_20190521_MT_17BHSD12_10_1-2_1) as TXT format.

Data_Figure 4_Impact of 17β‑HSD12, the 3‑ketoacyl‑CoA reductase of long‑chain fatty acid synthesis, on breast cancer cell proliferation and migration

Data of figure 4 from Impact of 17β‑HSD12, the 3‑ketoacyl‑CoA reductase of long‑chain fatty acid synthesis, on breast cancer cell proliferation and migration Dataset (doi: 10.1007/s00018-019-03227-w) contains the original figure as TIF-format (10.1194_jlr.M092908_Fig. 4). Corresponding raw data obtained from: a1/2) Migration potential as five files in CSV format (31003A-179400_date_examiner_17BHSD12_16_3_1-5); b 1/2) Migration potential as four files in CSV format (31003A-179400_date_examiner_17BHSD12_16_4_1-4); c1/2/3) mRNA content analyzed by RT-PCR provided as four files in CSV format (31003A-179400_date_examiner_17BHSD12_1_4_1-4); cellomics HTC array scan analysis provided as three files in CSV format (31003A-179400_Date_examiner_17BHSD12_8_3-4_1-4); d) Migration potential as five files in CSV format (31003A-179400_ date_examiner_17BHSD12_16_5_1-5); e) mRNA content analyzed by RT-PCR provided as six files in CSV format (31003A-179400_date_examiner_17BHSD12_1_5_1-6); f) Migration potential as four files in CSV format (31003A-179400_date_examiner_17BHSD12_16_6_1-4), cellomics HTC array scan analysis provided as three files in CSV format (31003A-179400_Date_examiner_17BHSD12_8_5_1-5); g) ELISA measurement provided as four files in CSV format (31003A-179400_date_examiner_17BHSD12_20_1_1-4). All further experiment related information protocols and subsequent data analysis provided as 10 meta-data-files (31003A-179400_date_examiner_17BHSD12_8/16/1/20_dataset_M_1) as TXT format.

Data_supplemental figure 2_Impact of 17β‑HSD12, the 3‑ketoacyl‑CoA reductase of long‑chain fatty acid synthesis, on breast cancer cell proliferation and migration

Data of supplemental figure 2 from Impact of 17β‑HSD12, the 3‑ketoacyl‑CoA reductase of long‑chain fatty acid synthesis, on breast cancer cell proliferation and migration Dataset (doi: 10.1007/s00018-019-03227-w) contains the original figure as TIF format (10.1194_jlr.M092908_Fig. S2). Corresponding raw data obtained from cellomics HTC array scan analysis provided as seven files in CSV format (31003A-179400_Date_examiner_17BHSD12_8_11-12_1-4) All further experiment related information protocols as meta-data-files (31003A-179400_date_examiner_17BHSD12_8_11-12_M_1) as TXT format.

opencc-zeroAug 2020View details →

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Data from: Dynamics of diet-egg transfer of fatty acids in the teleost fish, red drum (Sciaenops ocellatus)

Eggs of marine organisms are increasingly being recognized as important components of marine food webs. The degree to which egg fatty acid profiles reflect maternal diet fatty acid profiles, and therefore the value of fatty acids in eggs as trophic biomarkers, depends on the species' reproductive strategy and the extent of modification of ingested fatty acids. We measured the dynamics of transfer of recently ingested fatty acids to spawned eggs in a batch-spawning teleost, red drum (Sciaenops ocellatus). Results of 21 dietshift experiments, from which the fatty acid profiles of the diets and eggs were compared, showed that 15 of 27 fatty acids measured (one saturated, two monounsaturated and 12 polyunsaturated fatty acids) in eggs were correlated with their levels in the recent diet, and the rate of incorporation into eggs was proportional to the magnitude of the diet shift. Large shifts in diet might occur naturally during spawningmigrations or when prey communities vary over time. Results of this study indicate that fatty acids in red drum eggs can be useful for studying adult diet and exploring trophic linkages in marine systems. This article is part of the theme issue 'The next horizons for lipids as 'trophic biomarkers': evidence and significance of consumer modification of dietary fatty acids'.

opencc-by-4.0Jun 2020View details →

Computational modelling of metal soap formation in historical oil paintings: the influence of fatty acid concentration and nucleus geometry on the induced chemo-mechanical damage.

Metal soap formation is one of the most wide-spread degradation mechanisms observed in historical oil paintings, affecting works of art from museum collections worldwide. Metal soaps develop from a chemical reaction between metal ions present in the pigments and saturated fatty acids, which are released by the oil binder. The presence of large metal soap crystals inside paint layers or at the paint surface can be detrimental for the visual appearance of artworks. Moreover, metal soaps can possibly trigger mechanical damage, ultimately resulting in flaking of the paint. This paper departs from a recently proposed computational model to predict chemo-mechanical degradation in historical oil paintings, as presented in Eumelen et al. (J Mech Phys Solids 132:103683, 2019). The model describes metal soap formation and growth, which are phenomena that are driven by the diffusion of saturated fatty acids and proceed by a nucleation process from a crystalline nucleus of small size. This results into a chemically-induced strain in the paint, which may promote crack nucleation and propagation. The proposed model is here used to investigate the effects of saturated fatty acid concentration and initial nucleus geometry on the amount of chemo-mechanical damage generated. Numerical simulations show that both factors have a marginal influence on the growth rate of the metal soap crystal, but play a significant role on the extent of fracture induced in the paint.

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The more the better: Fatty acids are predictive markers of honey bee, Apis mellifera, worker longevity

Fatty acids (FA), stemming from nutrition, form triglycerides that are key components for insect energy reserves. In managed Apis mellifera colonies, supplementary feeding is common practice, yet micronutrients and microbiota (i.e. B-vitamins and probiotics) are often neglected. Given that B-vitamins are obligate cofactors for FA metabolism, and probiotics likely play key roles as well (i.e. Lactobacillus spp. synthesize B-vitamins), understanding how they contribute to FA acquisition remains unknown. Indeed, FAs are established predictors to A. mellifera longevity, and as such, are a logical point of interest in long-lived "winter" bees, where A. mellifera colony losses typically occur. Here, in a hoarding cage trial, freshly emerged adult winter workers were exposed to antibiotics (ABX) to decouple innate benefits associated to native gut microbiota, or left unexposed to ABX (N=72 cages, N=2088 experimental workers). Subsequently, all workers were fed different diets containing either probiotics, B-vitamins, with replicate treatments given ad libitum access to pollen (mimicking real-hive scenarios) or left blank (control). At the end of the trial, a subsample (n=356) had their total FA contents analyzed using Gas Chromatography coupled to Flame Ionization Detector (GC-FID). Irrespective of dietary treatment, every worker contained all 11 identified FAs, aligning our results with a priori evidence and highlighting their underlying key roles for bee physiology and health. We show for the first time that B-vitamins alone did not improve the overall abundance of individual FAs (g), yet significant differences were associated with presence/absence of bacteria and/or access to pollen, reconfirming likely ties of microbiota aiding in nutrient breakdown of complex polysaccharides found in pollen. Finally, of clear importance, there was a positive significant correlation between total lipid content and worker longevity (+2.4 median day lifespan increase / mg of FA), thereby confirming the relevance of FAs for honey bee worker longevity.

opencc-zeroMar 2024View details →

Figure 5 in Variations in trophic niches of generalist predators with plant community composition as indicated by stable isotopes and fatty acids

Figure 5. Principal components analysis of the relative abundance (mol %, logit- transformed) of individual NLFAs of Trochosa ruricola using body size (small, large), flooding index (FI), plant species richness (SR), plant functional group richness (FG), presence of grasses (Gr), legumes (Leg), small herbs (SH) and tall herbs (TH) as supplementary variables.

Figure 4 in Variations in trophic niches of generalist predators with plant community composition as indicated by stable isotopes and fatty acids

Figure 4. Variations in δ15N signatures of Trochosa ruricola as affected by flooding index (P = 0.04, R2 = 0.12) and body size (small, large; P <0.01).