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16 results for “freeze-thaw cycle”
Trace metal, ion, and nutrient concentrations in aeolian samples subjected to experimental freeze-thaw cycles, collected from Taylor Valley, McMurdo Dry Valleys, Antarctica (2013-2016)
This data package contains measurements of trace metal, ion, and nutrient concentrations in aeolian samples collected from several locations throughout Taylor Valley in the McMurdo Dry Valleys of Antarctica during the 2013-2014, 2014-2015, and 2015-2016 austral summers. Samples were collected by the McMurdo Dry Valleys Long Term Ecological Research Program (MCM LTER) using Big Spring Number Eight (BSNE) isokinetic wind samplers located at Explorer’s Cove, Lake Fryxell at F6, East Lake Bonney, and Taylor Glacier. Samples were then subjected to experimental freeze-thaw cycles in a controlled laboratory setting to simulate supraglacial weathering processes and then analyzed to understand how freeze-thaw cycles affect nutrient, ion, and trace metal concentrations over time.
Data from: Decoupled responses of soil bacteria and their invertebrate consumer to warming, but not freeze-thaw cycles, in the Antarctic Dry Valleys
Altered temperature profiles resulting in increased warming and freeze–thaw cycle (FTC) frequency pose great ecological challenges to organisms in alpine and polar ecosystems. We performed a laboratory microcosm experiment to investigate how temperature variability affects soil bacterial cell numbers, and abundance and traits of soil microfauna (the microbivorous nematode Scottnema lindsayae) from McMurdo Dry Valleys, Antarctica. FTCs and constant freezing shifted nematode body size distribution towards large individuals, driven by higher mortality among smaller individuals. FTCs reduced both bacterial and nematode abundance, but bacterial cell numbers also declined under warming, demonstrating decoupled consumer–prey responses. We predict that higher occurrence of FTCs in cold ecosystems will select for large body size within soil microinvertebrates and overall reduce their abundance. In contrast, warm temperatures without FTCs could lead to divergent responses in soil bacteria and their microinvertebrate consumers, potentially affecting energy and nutrient transfer rates in soil food webs of cold ecosystems.
Impact of Freeze-thaw Cycles on Coatings Applied to the Surface of Alkali-activated Materials
<p><span>This paper focuses on the possibility of applying selected types of coatings (synthetic and epoxy) on the surface of alkali-activated materials based on finely ground granulated blast furnace slag with an admixture of cement by-pass dust and silica fly ash. Admixtures represent 30% of the binder component (15% fly ash, 15% cement by-pass dust). The mixture is activated with anhydrous disodium metasilicate. The samples were categorized into two series samples stored in water and samples wrapped in foil. Following this, the surface of these two series was modified by two methods, namely brushing with a steel brush and roughening with a diamond wheel. The properties related to the adhesion of the coating to the surface were mainly investigated before and after 100 freeze-thaw cycles. The cross-cut method and pull-off test for adhesion determined the adhesion. The cross-cut test found that the synthetic coating was less susceptible to surface modification than the epoxy coating. The samples were exposed to 100 freeze-thaw cycles. Then, the cross-cut method was applied to the coated roughened surface and the results of this test were classified into category 3. During the determination of the adhesion of the coatings by the pull-off test on the roughened surface for both methods of sample storage, the character of breakage for the synthetic coating was of the cohesive type, thus it was the tensile strength of the materials, while for the epoxy coating, it was the adhesive breakage, thus it was the adhesion between the surface and the coating.</span></p>
Dataset for the Figure 2 in manuscript "Numerical study of coupled water and vapour flow, heat transfer, and solute transport in variably-saturated deformable soil during freeze-thaw cycles"
<p>This dataset includes the gathered experimental measurements of a freezing test by Wu (2017) for the model's verification shown in Figure 2 of the manuscript entitled 'Numerical study of coupled water and vapour flow, heat transfer, and solute transport in variably-saturated deformable soil during freeze-thaw cycles'' by Huang, X., and Rudolph, D.L.</p>
Data from: Decoupled responses of soil bacteria and their invertebrate consumer to warming, but not freeze-thaw cycles, in the Antarctic Dry Valleys
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Repeated freeze-thaw cycles increase extractable, but not total, carbon and nitrogen in a Maine coniferous soil
This dataset contains processed data for the publication Patel et al. 2021. "Repeated freeze-thaw cycles increase extractable, but not total, carbon and nitrogen in a Maine coniferous soil". Geoderma. https://doi.org/10.1016/j.geoderma.2021.115353. Northeastern North America has been experiencing warmer winters with reduced snow accumulation, with more frequent winter freeze-thaw cycles. We conducted a laboratory experiment to investigate how increased frequency of freeze-thaw cycles (FTC) would alter soil C and N availability. Organic (O) and mineral (B) horizon soils were collected from a coniferous forest in Maine, processed to exclude roots, and then frozen in the laboratory (-10 °C) with one (FTC-1), two (FTC-2), or six (FTC-6) thaw periods (+5 °C). Soils were analyzed for extractable ammonium (NH4-N), water extractable organic carbon (WEOC), carbon dioxide flux (respiration), and total C and N. Extractable NH4-N increased following FTC (all levels), for both horizons. While WEOC concentrations did not change for FTC vs. control, the WEOC in O horizons had a lower SUVA254 in FTC soils compared to control, indicating a stronger microbial influence (i.e., microbial cell lysis) in these soils after FTC. Respiration in O horizon soils decreased post-incubation and did not differ between FTC and Control soils. In the B horizon, however, FTC soils showed greater respiration than Control soils, suggesting that the newly available nutrients may have stimulated microbial activity. In contrast to these results, total C and N remained unaltered by FTC, presumably because the FTC disturbances represented mostly a translocation of C and N from one pool into another, and losses due to respiration were too small to significantly influence the large TC and TN pools. The effect of FTC on NH4-N did not change with FTC frequency, suggesting that a single FTC is sufficient to alter both C and N availability and/or quality, and that additional FTC may not have a significant further
Thermal adaptations to extreme freeze-thaw cycles in the high tropical Andes
<p>Temperature plays a key role in the biology of ectotherms, including anurans, which are found at higher elevations in the tropics than anywhere in the temperate zone. High-elevation tropical environments are characterized by extreme daily thermal fluctuation including high daily maxima and nightly freezing. Our study investigated the contrasting operative temperatures of the anurans <i>Telmatobius marmoratus </i>and<i> Pleurodema marmoratum</i> in different environmental contexts at the same elevation and biome above 5200 meters. <i>Telmatobius marmoratus</i> avoids extremes of daily temperature fluctuation by utilizing thermally buffered aquatic habitat at all life stages, with minimal operative temperature variation (range: 4.6–8.0°C).<i> Pleurodema marmoratum</i>, in contrast, experienced operative temperatures from -3.5 to 44°C and has one of the widest thermal breadths reported for any tropical frog, from >32°C (critical thermal maximum) to surviving freezing periods of 1 hr and 6 hr down to -3.0°C. Our findings expand experimental evidence of frost tolerance in amphibians to the widespread Neotropical family Leptodactylidae, the first such evidence of frost tolerance in a tropical amphibian. Our study identifies three strategies (wide thermal tolerance breadth, use of buffered microhabitats, and behavioral thermoregulation), which allow these tropical frogs to withstand the current wide daily thermal fluctuation above 5000 masl and which may help them adapt to future climatic changes.</p>
Quantitatively Monitoring of Seasonal Frozen Ground Freeze-thaw Cycle Using Ambient Seismic Noise Data
<p>This is the electronic supplemental data for the publication entitled </p> <p>"<strong>Quantitatively Monitoring of Seasonal Frozen Ground Freeze-thaw Cycle Using Ambient Seismic Noise Data</strong>"</p> <p>submitted to <strong>Seismological Research Letters (SRL)</strong>. </p> <p>The names of the compressed files represent the experiment number and station number. For example, "1_2" indicates data collected from the second station during the first experiment. Each compressed file contains seismic raw data in the ".SAC" format. The filenames include the UTC end time of data collection. For instance, "453003616.00000001.2021.10.20.06.40.22.000.z.sac" indicates that data collection ended at 06:40:22 on October 20, 2021. Each complete .sac file contains 4 days of data with a sampling interval of 0.002 seconds.</p>
A Randomized Controlled Study of Prolonging the Time of Progesterone Supplementation to Improve the Pregnancy Outcome of Single Day 6 Blastocyst Transfer of Freeze-thaw Cycle
ClinicalTrials.gov study NCT04938011. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Thermal adaptations to extreme freeze-thaw cycles in the high tropical Andes
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The effect of freeze-thaw cycles on gene expression levels in lymphoblastoid cell lines
GEO Series GSE58942. Homo sapiens. 187 samples. Type: Expression profiling by array.
Data from: Indices of immune function used by ecologists are mostly unaffected by repeated freeze-thaw cycles and methodological deviations
Background: Over the past couple of decades, measuring immunological parameters has become widespread in studies of ecology and evolution. A combination of different immunological indices is useful for quantifying different parts of the immune system and comprehensively assessing immune function. Running multiple immune assays usually requires samples to be repeatedly thawed and re-frozen. There is some evidence that repeated freezing and thawing can affect assay results, but this has never been comprehensively studied in some common ecological immunology assays. We tested the effect of multiple (1, 2, 3, 4, 5, 10) freeze-thaw cycles on the results of four commonly used immunological assays: haemolysis-haemagglutination titres, haptoglobin concentration, bacterial killing capacity and total immunoglobulins (IgY). We tested five different bird species from four different bird orders (Passeriformes, Columbiformes, Charadriiformes and Galliformes), and we included both captive and free-living individuals. In addition, we tested for haptoglobin concentrations and the haemolysis-haemagglutination assay if re-analysing samples 1 year apart led to different results. For the haemolysis-haemagglutination assay we also tested two different sources of rabbit blood, and we compared untreated microtitre plates with plates that were "blocked" to prevent nonspecific interactions between the plate and assay reagents. Results: Repeated freezing and thawing of plasma had no effect on lysis titres, haptoglobin concentrations, bacterial killing capacity, or total immunoglobulin levels. Agglutination titres were unaffected by up to five cycles but were lower after ten freeze-thaw cycles. For the haemolysis-haemagglutination assay and haptoglobin concentrations, re-analysing samples 1 year apart yielded highly correlated data. For the haemolysis haemagglutination assay,thesourceofrabbit blood did not influence the results, and the untreated vs. blocked plates differed slightly overall, but at the individual level assay results were highly correlated. Using different rabbit blood sources or different types of microtitre plates yielded highly correlated data.Conclusions: Our data suggest that repeated freeze-thaw cycles do not impair assay results to the point of influencing ecological or evolutionary conclusions. Plasma samples can be safely stored in one tube and thawed repeatedly for different assays. Nevertheless, we recommend consistent treatment of samples in terms of freeze-thaw cycles or other laboratory treatments to minimize the potential for introducing a systematic bias.
Effect of Low Molecular Weight Heparin in Freeze-thaw Embryo Transfer Cycles in Women With Recurrent Implantation Failure
ClinicalTrials.gov study NCT06991530. IPD Sharing: NO. Countries: 1. Publications: 0.
Data from: Indices of immune function used by ecologists are mostly unaffected by repeated freeze-thaw cycles and methodological deviations
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Tumor analysis: Freeze-thawing cycle of triple negative breast cancer cells changes tumor CD24/CD44 profile and percentage of tumor-infiltrating immune cells
GEO Series GSE114359. Homo sapiens. 6 samples. Type: Expression profiling by array.
Multiple freeze-thaw cycles lead to a loss of consistency in poly(A)-enriched RNA sequencing
GEO Series GSE150097. Homo sapiens. 108 samples. Type: Expression profiling by high throughput sequencing; Other.
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