Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
19
datasets available to search
ShareScore release 0.9.0
Dataset results
19 results for “genetic manipulation”
Data from: Aging and memory are altered by genetically manipulating lactate dehydrogenase in the neurons or glia of flies
<p>The astrocyte-neuron lactate shuttle hypothesis posits that glial-generated lactate is transported to neurons to fuel metabolic processes required for long-term memory. Although studies in vertebrates have revealed that lactate shuttling is important for cognitive function, it is uncertain if this form of metabolic coupling is conserved in invertebrates or is influenced by age. Lactate dehydrogenase (Ldh) is a rate-limiting enzyme that interconverts lactate and pyruvate. Here we genetically manipulated expression of <em>Drosophila</em> <em>melanogaster</em> lactate dehydrogenase (dLdh) in neurons or glia to assess the impact of altered lactate metabolism on invertebrate aging and long-term courtship memory at different ages. We also assessed survival, negative geotaxis, brain-neutral lipids (the core component of lipid droplets), and whole head metabolites. Both upregulation and downregulation of dLdh in neurons resulted in decreased survival and memory impairment with age. Glial downregulation of dLdh expression caused age-related memory impairment without altering survival, while upregulated glial dLdh expression lowered survival without disrupting memory. Both neuronal and glial dLdh upregulation increased neutral lipid accumulation. We provide evidence that altered lactate metabolism with age affects the tricarboxylic acid (TCA) cycle, 2-hydroxyglutarate (2HG), and neutral lipid accumulation. Collectively, our findings indicate that the direct alteration of lactate metabolism in either glia or neurons affects memory and survival but only in an age-dependent manner. </p>
Data from: Aging and memory are altered by genetically manipulating lactate dehydrogenase in the neurons or glia of flies
Open the record for dataset details and reuse information.
Data from: Parasite-manipulated host dispersal: evidence from population genetics and mark-recapture experiment
Open the record for dataset details and reuse information.
Data from: Parental genetic similarity and offspring performance in blue tits in relation to brood size manipulation
Open the record for dataset details and reuse information.
Data from: Social interactions predict genetic diversification: an experimental manipulation in shorebirds
Open the record for dataset details and reuse information.
Data from: Enhanced flight performance by genetic manipulation of wing shape in Drosophila
Insect wing shapes are remarkably diverse and the combination of shape and kinematics determines both aerial capabilities and power requirements. However, the contribution of any specific morphological feature to performance is not known. Using targeted RNA interference to modify wing shape far beyond the natural variation found within the population of a single species, we show a direct effect on flight performance that can be explained by physical modelling of the novel wing geometry. Our data show that altering the expression of a single gene can significantly enhance aerial agility and that the Drosophila wing shape is not, therefore, optimized for certain flight performance characteristics that are known to be important. Our technique points in a new direction for experiments on the evolution of performance specialities in animals.
Data from: Mind the gap: genetic manipulation of basicranial growth within synchondroses modulates calvarial and facial shape in mice through epigenetic interactions
Phenotypic integration patterns in the mammalian skull have long been a focus of intense interest as a result of their suspected influence on the trajectory of hominid evolution. Here we test the hypothesis that perturbation of cartilage growth, which directly affects only the chondrocranium during development, will produce coordinated shape changes in the adult calvarium and face regardless of mechanism. Using two murine models of cartilage undergrowth that target two very different mechanisms, we show that strong reduction in cartilage growth produces a short, wide, and more flexed cranial base. This in turn produces a short, wide face in both models. Cranial base and face are already correlated early in ontogeny, and the relationship between these modules gains structure through postnatal growth and development. These results provide further evidence that there exist physical interactions between developing parts of the phenotype that produce variation at a distance from the actual locus upon which a particular selective pressure is acting. Phenotypic changes observed over the course of evolution may not all require adaptationist explanations; rather, it is likely that a substantial portion of observed phenotypic variation over the history of a clade is not directly adaptive but rather a secondary consequence of some local response to selection.
Data from: Mind the gap: genetic manipulation of basicranial growth within synchondroses modulates calvarial and facial shape in mice through epigenetic interactions
Open the record for dataset details and reuse information.
Data from: Enhanced flight performance by genetic manipulation of wing shape in Drosophila
Open the record for dataset details and reuse information.
Multiplexed single cell mRNA sequencing of monolayer- and organoid-system differentiated cells derived from human iPSC with and without NKX2-5 genetic manipulations
GEO Series GSE163619. Homo sapiens. 3 samples. Type: Expression profiling by high throughput sequencing.
Expansion, in vivo-ex vivo cycling and genetic manipulation of primary human hepatocytes
GEO Series GSE130219. Homo sapiens. 7 samples. Type: Expression profiling by high throughput sequencing.
An inducible genetic tool for tracking and manipulating specific microglial states in development and disease
GEO Series GSE264553. Mus musculus. 2634 samples. Type: Expression profiling by high throughput sequencing.
Whole genome CRISPR screening identifies genetic manipulations to reduce immune rejection of stem cell-derived islets
GEO Series GSE200104. Homo sapiens. 23 samples. Type: Expression profiling by high throughput sequencing; Other.
Mobile-CRISPRi as a tool for genetic manipulation in the intracellular pathogen Piscirickettsia salmonis
GEO Series GSE309378. Piscirickettsia salmonis. 6 samples. Type: Expression profiling by high throughput sequencing.
Experimental manipulation of selfish genetic elements links genes to microbial community function
<p>Microbial communities underpin earth’s biological and biogeochemical processes, but their complexity hampers understanding. Here we draw upon predictions from the theory of selfish genetic elements (SGEs), combined with approaches from experimental evolution, comparative metagenomics and biochemistry, and show how naturally occurring SGEs can be used to manipulate genes that underpin community function. Communities comprising hundreds of bacterial genera established from garden compost were propagated with bi-weekly transfer for one year in nitrogen-limited minimal medium with cellulose (paper) as sole carbon source. At each transfer, SGEs from one set of independent communities were collected, pooled and redistributed among this same set of communities (horizontal treatment). A control was included in which SGEs never moved beyond the community in which they were originally present (vertical treatment). SGEs along with genes of ecological relevance were rapidly amplified across horizontal communities and their dynamics tracked. Enrichment of genes implicated in nitrogen metabolism, and particularly ammonification, led to biochemical assays that showed a significant functional difference between communities subject to horizontal versus vertical treatments. This simple experimental strategy offers a powerful new approach for unravelling dynamical processes underpinning microbial community function.</p>
Genetic Manipulation of Putrescine Biosynthesis Reprograms the Cellular Transcriptome and the Metabolome
GEO Series GSE79420. Populus euphratica; Populus tremula x Populus alba; Populus trichocarpa x Populus deltoides; Populus maximowiczii x Populus nigra. 24 samples. Type: Expression profiling by array.
Expression data from the bladders of carcinogen-treated mice with genetic Hh pathway manipulation
GEO Series GSE60654. Mus musculus. 6 samples. Type: Expression profiling by array.
Global gene expression in response to genetic and growth factor manipulation of TWIST1 expression and function
GEO Series GSE76158. Homo sapiens. 4 samples. Type: Expression profiling by array.
Expression data from Drosophila melanogaster E2f/Rb genetic pathway manipulation
GEO Series GSE7655. Drosophila melanogaster. 18 samples. Type: Expression profiling by array.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.