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4 results for “giant unilamellar vesicles”
Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis - Imaging Flow Citometry Data
<p>Imaging flow citometry (IFC) datasets analysed in "Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis" (under revision).</p> <p>The folders contain acquisitions of giant unilamellar vesicles (GUVs) for lipid exchange and content exchange controls, with file naming convention DATE_SAMPLE_REPLICATE.rif, content exchange is indicated by CE samples in the 20230228_CE.zip folder, lipid exchange by LE samples in the 20221222_LE.zip folder. 24 samples per set are included, triplicates of isolated P1 (DOPE Af488 0.6% in LE; Dex-Af488 40 uM for CE), P2 (DOPE Cy50.6% in LE; Dex-Af647 10 uM for CE), NC (P1 + P2 1:1), PC (DOPE Af488 0.3% + DOPE Cy5 0.3 in LE; Dex-Af488 20 uM + Dex-Af647 5 uM for CE), and M samples numbered 1 to 4, prepared by mixing P1, P2 and PC in different ratios (M1= 1:1:1; M2= 1:1:0.5; M3= 1:1:0.1; M4= 1:1:0.05).</p> <p>Only .rif files are provided, they have to be elaborated via compensation and application of an analysis template using the Amnis IDEAS software. Compensation matrices for lipid exchange (20230217_LEcom.ctm) and content exchange (20230217_CEcomp.ctm) are included, as well as the analysis template (Lipid_exchange_analysis_6.2.ast). Gating in the latter may have to be adjusted to analyse LE and CE experiments.</p> <p>10000 objects in the GUV population or 50000 objects in total were acquired in each file. The files were elaborated in batch mode, outputting the statistic reports (Statistics report CE.txt for CE; Statistics report LE.txt for LE) that were elaborated using an R scirpt (included, IFC_analysis.R) </p>
Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis - Microscopy Data
<p>Microscopy dataset of multipoint-multichannel images of giant unilamellar vesicles (GUVs) suspensions analysed in "Quantification of Giant Unilamellar Vesicle Fusion Products by High-Throughput Image Analysis" (under revision).</p> <p>Three folders concerning different sections of the work are included. "preliminary analysis.zip" contians the raw files and analysis scripts for recall computation and imaging setup optimization as described in the paper. Timelapse data was excluded due to file size restrictions (available upon request at the corresponding authors of the work). "IFC comparison.zip" contains raw files and analysis scripts used to optimize colocalization computation in lipid exchange and content exchange experiments. "GUV fusion analysis" contains raw files and analysis scripts for the quantification of lipid and content exchange upon sodium chloride-induced aggregation.</p> <p>Further details on the analysis are provided in the paper. The R scripts require files saved upon analysis of the raw files by the ImageJ macro "CE_analysis_CPU.ijm" included here. The R environment of the complete analysis are included in each folder to provide easier access to the elaborated data.</p>
CELLOIDS: pH-responsive DOPC Giant Unilamellar Vesicles with and without Linoleic Acid
<p>As part of the CELLOIDS project, one of our main research focuses is exploring the feasibility of making the microrobot's membrane responsive to a pH gradient. Specifically, we investigated the incorporation of linoleic acid into Giant unilamellar vesicles composed of DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine).</p> <p>The provided dataset compiles the diameters of liposomes prepared with DOPC (3.18 mM), DOPC:LA (linoleic acid) at a molar ratio of 100:1, and DOPC:LA at a molar ratio of 50:1. These liposomes were placed in buffers at both pH 6 and pH 7, simulating conditions similar to the tumor microenvironment. Our objective was to assess the stability of these liposomes under these conditions.</p> <p>In detail, we carefully prepared liposomes with varying molar ratios of DOPC and linoleic acid to determine the optimal amount of linoleic acid that induces membrane deformation while maintaining the structural integrity of the liposomes in acidic solutions. Subsequently, we subjected the liposomes to testing at the specified concentrations and compared their behavior under two different pH values. Finally, we observed the samples using a phase-contrast microscope immediately after the addition of the solutions and after a one-hour incubation period.</p>
Dimensions, stability and deformability of DOPC-cholesterol Giant Unilamellar Vesicles formed by droplet transfer – Extended Data
<p>This dataset contains the Underlying Data to the paper “ Dimensions, stability and deformability of DOPC-cholesterol Giant Unilamellar Vesicles formed by droplet transfer”.</p> <ul> <li> “deformation_size” folder containing scatter plots of σ with respect to GUVs rest radii <ul> <li>sd_deform_scatter_H1</li> <li>sd_deform_scatter_H2</li> <li>sd_deform_scatter_H3</li> </ul> </li> <li>“magnetic_device_support” folder containing the .stl files for 3D-printing the magnets-support of the magnetic device <ul> <li>magnetic_device_support_part1</li> <li>magnetic_device_support_part2</li> </ul> </li> <li>“size_distribution_magnetic” folder containing size distribution histograms comparing 100:0 DOPC:cholesterol and 60:40 DOPC:cholesterol samples, under the application of magnetic fields <ul> <li>sd_magnetic_size_dist_allfields</li> <li>sd_magnetic_size_dist_H1</li> <li>sd_magnetic_size_dist_H2</li> <li>sd_magnetic_size_dist_H3</li> </ul> </li> <li>“size_distribution_T0vsON” folder containing size distribution histograms comparing pristine samples (t<sub>0</sub>) and samples after overnight storage (ON), for different DOPC:cholesterol ratios <ul> <li>sd_size_dist_60_40</li> <li>sd_size_dist_71_29</li> <li>sd_size_dist_85_15</li> <li>sd_size_dist_100_0</li> </ul> </li> </ul>
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