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124 results for “hamster”
Raw data to accompany the manuscript 'Data for Engineering Lipid Metabolism of Chinese Hamster Ovary (CHO) Cells for Enhanced Recombinant Protein Production' published in the Journal Data in Brief
<p>This repository consists of the raw western blot, microscopy and mass spectrometry data to accompany the manuscript 'Data for Engineering Lipid Metabolism of Chinese Hamster Ovary (CHO) Cells for Enhanced Recombinant Protein Production' published in the Journal Data in Brief and associated with the article '<a href="https://www.ncbi.nlm.nih.gov/pubmed/31805379">Engineering of Chinese hamster ovary cell lipid metabolism results in an expanded ER and enhanced recombinant biotherapeutic protein production</a>' published in the journal Metabolic Engineering (see DOI: 10.1016/j.ymben.2019.11.007). </p> <p>The western blot raw file is associated with Figure 1a and 1b of the Data in Brief manuscript.</p> <p>The confocal microscopy raw image files (x3) are associated with Figure 1c of the Data in Brief manuscript.</p> <p>The mass spectrometry files are the raw data that refers to the samples presented in Figure 5 of the Data in Brief manuscript. Files are labelled as in the Data in Brief and Metabolic Engineering manuscripts. The file name structures is as follows;</p> <p>CHO-Controlpoolai</p> <p>Where 'a' represents replicate 'a' of three biological replicates and 'i' refers to mass spectrometry technical analysis 1 of 3 technical analyses of each replicate (thus for each cell pool or line there are three biological replicates that are each analysed in triplicate such that there are 9 raw mass spectrometry files for each cell pool or line).</p> <p>All the mass spectrometry files are found in the compressed (zip) file named mass_spectrometry_raw_files_archive.zip</p>
Data from: "Alteration of the gut microbiota's composition and metabolic output correlates with COVID-19-like severity in obese NASH hamsters"
<p>This dataset contains all data collected and used for the publication : "Alteration of the gut microbiota’s composition and metabolic output correlates with COVID-19-like severity in obese NASH hamsters". Besides the Readme, it contains 11 files.</p> <p><br> Excel files with classification (i.e. genes according to their fold induction or repression) are provided. Data include different conditions with varying number of samples per group. Data are structured according to employed methods and then stratify the data obtained within the individual work packages.</p>
Predictive nano-QSAR modeling of the cytotoxicity using epithelial cells obtained from Chinese hamster ovary (CHO-K1 cell line) for hybrid TiO2-based nanomaterials
<p>Results obtained from developed model indicated that the cytotoxicity of hybrid TiO2-based nanomaterials is related to additive electronegativity (χmix) of studied nanomaterials that are indirectly related to the electron generation and ROS formation. ROS production is the most common toxicity cause as discussed in the literature in the case of nanoparticles. The high efficiency of surface modified TiO2-based semiconductors can be attributed to the involvement of TiO2 band gap (Eg) excitation and absence of noble metals at the TiO2 surface. It can be expected that noble metals (i.e. Pd/Pt) may trap holes (h+), at the same time photo-generated electrons can be then transferred from the valence band to the conduction band of TiO2 and to its surface where redox processes were initiated. Thus, observed reduction of the electron–hole pair recombination influences the reactive oxygen species (ROS) formation and the photocatalytic redox process initiation.</p> <p>Since the electronegativity was positively correlated with the cytotoxicity it can be expected that some ions are released from the TiO2 surface easier than others.</p>
Annex 2: Supplementary Tables to 'Understanding Chinese hamster translation at sub-codon resolution'
<p>Annex 2 to PhD thesis titled 'Understanding Chinese hamster ovary cell translation at sub-codon resolution'. </p>
Figure 2 in Rediscovered after half a century: a new record of the grey dwarf hamster, Cricetulus migratorius (Mammalia: Cricetidae), in Romania
Figure 2. Mandible of Cricetulus migratorius in comparison to other hamster species present in Romania (from top to bottom: A. Cricetulus migratorius, Botoşani, 10 Mar 2007; B. Mesocricetus newtoni, Măcin, 21 Oct 2013; C. Cricetus cricetus, Jigodin Bai, 02 May 2010, all from owl pellets).
Metaproteomics reveals age-specific alterations of gut microbiome in hamsters with SARS-CoV-2 infection
<p><span>The gut microbiome's pivotal role in health and disease is well-established. SARS-CoV-2 infection often causes gastrointestinal symptoms and is associated with changes of the microbiome in both human and animal studies. While hamsters serve as important animal models for coronavirus research, there exists a notable void in functional characterization of their microbiomes with metaproteomics. In this study, we present a workflow for analyzing the hamster gut microbiome, including a metagenomics-derived hamster gut microbial protein database and a data-independent acquisition metaproteomics method. Using this workflow, we identified 32419 protein groups from the fecal microbiomes of young and old hamsters infected with SARS-CoV-2 . We showed age-specific changes in the expressions of microbiome functions and host proteins associated with microbiomes, providing further functional insight into the dysbiosis and aberrant cross-talks between the microbiome and host in SARS-CoV-2 infection. Altogether this study established and demonstrated the capability of metaproteomics for the study of hamster microbiomes.<span> </span></span></p>
Sex-specific endocrine regulation of seasonal aggression in Siberian hamsters
<p>Coordinating physiological and behavioural processes across the annual cycle is essential in enabling individuals to maximize fitness. While the mechanisms underlying seasonal reproduction and its associated behaviours are well-characterized, fewer studies have examined the hormonal basis of non-reproductive social behaviours (e.g., aggression) on a seasonal timescale. Our previous work suggests that the pineal hormone melatonin facilitates a 'seasonal switch' in neuroendocrine regulation of aggression in male and female Siberian hamsters (Phodopus sungorus), specifically by acting on the adrenal glands to increase the production of the androgen dehydroepiandrosterone (DHEA) during the short-day (SD) photoperiods of the non-breeding season. Here, we provide evidence that the activity of 3β-hydroxysteroid dehydrogenase/∆5-∆4 isomerase (3β-HSD), a key enzyme within the steroidogenic pathway that mediates DHEA synthesis and metabolism, varies in a sex-specific and melatonin-dependent manner. Although both male and female hamsters displayed increased aggression in response to SDs and SD-like melatonin, only males showed an increase in adrenal 3β-HSD activity. Conversely, SD and melatonin-treated females exhibited reductions in both adrenal and neural 3β-HSD activity. Collectively, these results suggest a potential role for 3β-HSD in modulating non-breeding aggression and, more broadly, demonstrate how distinct neuroendocrine mechanisms may underlie the same behavioural phenotype in males and females.</p>
Diet-induced obesity and NASH impair disease recovery in SARS-CoV-2-infected golden hamsters
<p>Obese patients with nonalcoholic steatohepatitis (NASH) are prone to severe forms of COVID-19. There is an urgent need for new treatments that lower the severity of COVID-19 in this vulnerable population. To better replicate the human context, we set up a diet-induced model of obesity associated with dyslipidemia and NASH in the golden hamster (known to be a relevant preclinical model of COVID-19). A 20-week, free-choice diet induces obesity, dyslipidemia and NASH (liver inflammation and fibrosis) in golden hamsters. Obese NASH hamsters have higher blood and pulmonary levels of inflammatory cytokines. In the early stages of a SARS-CoV-2 infection, the lung viral load and inflammation levels were similar in lean hamsters and obese NASH hamsters. However, obese NASH hamsters showed worse recovery (i.e. less resolution of lung inflammation 10 days post-infection (dpi), and lower body weight recovery on dpi 25). Obese NASH hamsters also exhibited higher levels of pulmonary fibrosis on dpi 25. Unlike lean animals, obese NASH hamsters infected with SARS-CoV-2 presented long-lasting dyslipidemia and systemic inflammation. Relative to lean controls, obese NASH hamsters had lower serum levels of angiotensin-converting enzyme 2 activity and higher serum levels of angiotensin II - a component known to favor inflammation and fibrosis. Even though the SARS-CoV-2 infection resulted in early weight loss and incomplete body weight recovery, obese NASH hamsters showed sustained liver steatosis, inflammation, hepatocyte ballooning, and marked liver fibrosis on dpi 25.<strong> </strong>We conclude that diet-induced obesity and NASH impair disease recovery in SARS-CoV-2-infected hamsters. This model might be of value in characterizing the pathophysiologic mechanisms of COVID-19 and in evaluating the efficacy of treatments for the severe forms of COVID-19 observed in obese patients with NASH.</p>
golden_hamster:v24.8.1
<p>kraken2 DB built for Golden hamster (Mesocricetus Auratus) with masking option. Built in Aug 2024 based on latest assembly at that time:</p> <p> </p> <p>https://ftp.ncbi.nlm.nih.gov/genomes/refseq/vertebrate_mammalian/Mesocricetus_auratus/latest_assembly_versions/GCF_017639785.1_BCM_Maur_2.0/GCF_017639785.1_BCM_Maur_2.0_genomic.fna.gz</p>
Sex-specific endocrine regulation of seasonal aggression in Siberian hamsters
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Year-round evaluation of the conservation potential of seed-rich field margins under agri-environmental schemes for farmland birds, European hares, and common hamsters
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Gut microbiota sequences of the long-tailed dwarf hamster (Cricetulus longicaudatus) using 16S rDNA
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Cadmium exposure induces changes in intestinal microbial structure and metabolic function in long-tailed hamsters (Cricetulus longicaudatus)
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Seasonal patterns of melatonin alter aggressive phenotypes of female Siberian hamsters
<p>Many animal species exhibit year-round aggression, a behaviour that allows individuals to compete for limited resources in their environment (e.g., food and mates).<br> Interestingly, this high degree of territoriality persists during the non-breeding season, despite low levels of circulating gonadal steroids (i.e., testosterone [T] and oestradiol<br> [E<sub>2</sub>]). Our previous work suggests that the pineal hormone melatonin mediates a 'seasonal switch' from gonadal to adrenal regulation of aggression in Siberian hamsters<br> (<em>Phodopus sungorus</em>); solitary, seasonally breeding mammals that display increased aggression during the short, 'winter-like' days (SDs) of the non-breeding season. To test<br> the hypothesis that melatonin elevates non-breeding aggression by increasing circulating and neural steroid metabolism, we housed female hamsters in long days (LDs)<br> or SDs, administered them timed or mis-timed melatonin injections (mimic or do not mimic a SD-like signal, respectively), and measured aggression, circulating hormone<br> profiles, and aromatase (ARO) immunoreactivity in brain regions associated with aggressive or reproductive behaviours (paraventricular hypothalamic nucleus [PVN],<br> periaqueductal gray [PAG], and ventral tegmental area [VTA]). Females that were responsive to SD photoperiods (SD-R) and LD females given timed melatonin injections<br> (Mel-T) exhibited gonadal regression and reduced circulating E<sub>2</sub>, but increased aggression and circulating dehydroepiandrosterone (DHEA). Furthermore, aggressive challenges differentially altered circulating hormone profiles across seasonal phenotypes; reproductively inactive females (ie, SD-R and Mel-T females) reduced circulating<br> DHEA and T, but increased E<sub>2</sub> after an aggressive interaction, whereas reproductively active females (i.e., LD females, SD non-responder females, and LD females given mis-timed melatonin injections) solely increased circulating E<sub>2</sub>. Although no differences in neural ARO abundance were observed, LD and SD-R females showed distinct associations between ARO cell density and aggressive behaviour in the PVN, PAG, and VTA. Taken together, these results suggest that melatonin increases non-breeding<br> aggression by elevating circulating steroid metabolism after an aggressive encounter and by regulating behaviourally relevant neural circuits in a region-specific manner.</p>
Characterization and mutagenesis of Chinese hamster ovary cells endogenous retroviruses to inactivate viral particle release
<p>The Chinese hamster ovary (CHO) cells used to produce biopharmaceutical proteins are known to contain type‐C endogenous retrovirus (ERV) sequences in their genome and to release retroviral‐like particles. Although evidence for their infectivity is missing, this has raised safety concerns. As the genomic origin of these particles remained unclear, we characterized type‐C ERV elements at the genome, transcriptome, and viral particle RNA levels. We identified 173 type‐C ERV sequences clustering into three functionally conserved groups. Transcripts from one type‐C ERV group were full‐ length, with intact open reading frames, and cognate viral genome RNA was loaded into retroviral‐like particles, suggesting that this ERV group may produce functional viruses. CRISPR‐Cas9 genome editing was used to disrupt the gag gene of the expressed type‐C ERV group. Comparison of CRISPR‐derived mutations at the DNA and RNA level led to the identification of a single ERV as the main source of the release of RNA‐loaded viral particles. Clones bearing a Gag loss‐of‐function mutation in this ERV showed a reduction of RNA‐containing viral particle release down to detection limits, without compromising cell growth or therapeutic protein production. Overall, our study provides a strategy to mitigate potential viral particle contaminations resulting from ERVs during biopharmaceutical manufacturing.</p>
Data from: Aggressive behaviours track transitions in seasonal phenotypes of female Siberian hamsters
Seasonally breeding animals exhibit profound physiological and behavioural responses to changes in ambient day length (photoperiod), including changes in reproductive function and territorial aggression. Species where aggression persists when gonads are regressed and circulating levels of gonadal hormones are low, such as Siberian hamsters (Phodopus sungorus) and song sparrows (Melospiza melodia), challenge the well-established framework that gonadal hormones are important mediators of aggression. A solution to this apparent paradox is that a season-specific increase in sensitivity to hormones in brain areas associated with aggression offsets low levels of gonadal hormones during periods of reproductive quiescence. To test this hypothesis, we manipulated photoperiod to induce natural fluctuations in seasonal phenotype across multiple stages of the annual reproductive cycle in female Siberian hamsters that display increased aggression during short-day reproductive quiescence, suggesting that behaviour persists independent of gonadal steroids. Females were housed in long "summer" days or short "winter" days for 10, 24 or 30 weeks to capture gonadal regression, transition back to a reproductively functional state and full gonadal recrudescence, respectively. Long-day animals maintained reproductive functionality and displayed low aggression across all time points. By week 10, short-day reproductively responsive females underwent gonadal regression and displayed increased aggression; non-responsive animals showed no such changes. At week 24, animals were in a transitional period and displayed an intermediate phenotype with respect to reproduction and aggression. By week 30, short-day females were fully recrudesced and returned to long-day-like levels of aggression. Consistent with our hypothesis, gonadally regressed females displayed decreases in 17β-oestradiol (oestradiol) levels, but site-specific increases in the abundance of brain oestrogen receptor-alpha (ERα) in regions associated with aggression, but not reproduction. Increased site-specific ERα may function as a compensatory mechanism to allow increased responsiveness to oestradiol in regulating aggression in lieu of high circulating concentrations of hormones. Collectively, these results broaden our understanding of how breeding phenology maps onto social behaviour and the mechanisms that have evolved to coordinate behaviours that occur in non-breeding contexts.
Characterisation of large transgene integrations in Chinese hamster ovary cells using a bioengineered mammalian transposase
<p>Supporting information for "Characterisation of large transgene integrations in Chinese hamster ovary cells using a bioengineered mammalian transposase"</p>
On following pages: 4. Golden Hamster (Mesocricetus auratus); 5. Ciscaucasian Hamster (Mesocricetus raddei); 6 Hamster (Cricetulus migratorius); 9. Long-tailed Dwarf Hamster (Cricetulus longicaudatus); 10. Striped Dwarf Hamster (Cricetulus alticola); 13. Tibetan Dwarf Hamster (Cricetulus kamensis); 14. Gansu Hamster (Cansumys canus); 15. Eversmann's Hamster (Allocricetulus eversmanni); 18. Common Hamster (Cricetus cricetus); 19. Long-clawed Mole (Ondatra zibethicus); 22. Western Heather Vole (Phenacomys intermedius); 23. Eastern Heather Vole (Phenacomys Tree Vole (Arborimus pomo); 27. Northern Bog Lemming (Synaptomys borealis); 28. Southern Bog Lemming (Synaptomys 31. Norway Brown Lemming (Lemmus lemmus); 32. Siberian Brown Lemming (Lemmus sibiricus); 33. Nearctic Brown. Brandt's Hamster (Mesocricetus brandti); 7. Romanian Hamster (Mesocricetus newton); 8. Gray Dwarf (Cricetulus barabensis); 11. Sokolov's Dwarf Hamster (Cricetulus sokolovi); 12. Ladakh Dwarf Hamster Greater Long-tailed Hamster (Tscherskia triton); 16. Mongolian Hamster (Allocricetulus curtatus); 17. Vole (Prometheomys schaposchnikowi); 20. Round-tailed Muskrat (Neofiber allen); 21. Common Muskrat ungava); 24. White-footed Vole (Arborimus albipes); 25. Red Tree Vole (Arborimus longicaudus); 26. Sonoma cooperi); 29. Wood Lemming (Myopus schisticolor); 30. Amur Brown Lemming (Lemmus amurensis); Lemming (Lemmus trimucronatus). in Cricetidae
On following pages: 4. Golden Hamster (Mesocricetus auratus); 5. Ciscaucasian Hamster (Mesocricetus raddei); 6 Hamster (Cricetulus migratorius); 9. Long-tailed Dwarf Hamster (Cricetulus longicaudatus); 10. Striped Dwarf Hamster (Cricetulus alticola); 13. Tibetan Dwarf Hamster (Cricetulus kamensis); 14. Gansu Hamster (Cansumys canus); 15. Eversmann's Hamster (Allocricetulus eversmanni); 18. Common Hamster (Cricetus cricetus); 19. Long-clawed Mole (Ondatra zibethicus); 22. Western Heather Vole (Phenacomys intermedius); 23. Eastern Heather Vole (Phenacomys Tree Vole (Arborimus pomo); 27. Northern Bog Lemming (Synaptomys borealis); 28. Southern Bog Lemming (Synaptomys 31. Norway Brown Lemming (Lemmus lemmus); 32. Siberian Brown Lemming (Lemmus sibiricus); 33. Nearctic Brown. Brandt's Hamster (Mesocricetus brandti); 7. Romanian Hamster (Mesocricetus newton); 8. Gray Dwarf (Cricetulus barabensis); 11. Sokolov's Dwarf Hamster (Cricetulus sokolovi); 12. Ladakh Dwarf Hamster Greater Long-tailed Hamster (Tscherskia triton); 16. Mongolian Hamster (Allocricetulus curtatus); 17. Vole (Prometheomys schaposchnikowi); 20. Round-tailed Muskrat (Neofiber allen); 21. Common Muskrat ungava); 24. White-footed Vole (Arborimus albipes); 25. Red Tree Vole (Arborimus longicaudus); 26. Sonoma cooperi); 29. Wood Lemming (Myopus schisticolor); 30. Amur Brown Lemming (Lemmus amurensis); Lemming (Lemmus trimucronatus).
Hamster Vaping Manuscript Supplement
<p>Manuscript supplemental results </p>
Removal of senescent cells reduces the viral load and attenuates pulmonary and systemic inflammation in SARS-CoV-2-infected, aged hamsters
<p>Older age is one of the strongest risk factors for coronavirus disease 2019 (COVID-19) morbidity and mortality. In an older adult, the chronic accumulation of senescent cells can interfere with the immune system and accentuate inflammation. Here, we sought to determine whether age-associated cellular senescence contributes to the severity of COVID-19 by studying the well-established golden hamster model of severe acute respiratory syndrome coronavirus (SARS-CoV-2)-driven lung disease. We found that aged hamsters (22 months of age) accumulate senescent cells in the lungs and that the senolytic drug ABT-263 (a Bcl-2-family inhibitor) depletes these cells at baseline and during a SARS-CoV-2 infection (when the senescent cell count is typically elevated). Relative to young hamsters (2 months of age), aged hamsters had a greater viral load during the acute phase of infection and displayed higher levels of fibrosis and worse body weight recovery during the post-acute phase. Interestingly, early treatment with ABT-263 was associated with a significantly lower pulmonary viral load and an amelioration of COVID-19-like lung disease in aged (but not young) animals. ABT-263 treatment of aged animals was also associated with lower pulmonary and systemic levels of senescence-associated secretory phenotype factors. We conclude that the removal of senescent cells via treatment with a senolytic reduces the pathologic severity of SARS-CoV-2 infection in aged hamsters. As several senolytics have recently moved into early-stage clinical trials, our present findings have clear clinical relevance.</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.