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8 results for “high-speed microscopy”
Dataset for the manuscript: Pixel-wise programmability enables dynamic high-SNR cameras for high-speed microscopy
<p>These are the data files used to generate the figures in the paper: Pixel-wise programmability enables dynamic high-SNR cameras for high-speed microscopy. DOI: 10.1101/2023.06.27.546748</p>
Data for: High-Speed 3D Imaging of Multiphase Systems: Applying SCAPE Microscopy to Analogue Experiments in Volcanology and Earth Sciences
<p>Microscale processes in three-phase suspensions (mixtures of gas, liquids, and solids) can affect the macroscale behavior of the whole suspension. To visualize these small-scale processes at high speed and in 3D, we use a recently developed imaging system: Swept Confocally-Aligned Planar Excitation (SCAPE) microscopy. This dataset contains 3D videos taken with SCAPE microscopy of experiments where different phases interact with each other. Each zipped folder contains raw data and processed data for a single experiment. "Case 1" experiments show CO2 bubbles growing on PMMA (acrylic) particles in sparkling water. The "Case 2" experiment shows water droplets suspended in canola oil and flowing through a porous medium made of packed PMMA particles. "Case 3" experiments show growth of injected air bubbles in particle suspensions (either glass beads in immersion oil, or PMMA particles in a refractive index matched liquid).</p> <p>All scaling parameters are provided in Table 1. "info.txt" files contain metadata for the processed hyperstacks.</p> <p>The experiments provided here are discussed in the following publication:<br> Oppenheimer, J.*, Patel, K.*, Lindoo, A., Hillman, E. M. C., and Lev, E.: High-Speed 3D Imaging of Multiphase Systems: Applying SCAPE Microscopy to Analogue Experiments in Volcanology and Earth Sciences. <em>Geochemistry, Geophysics, Geosystems.</em> (In press, 12/2020)</p> <p><br> </p>
High-speed video microscopy analysis of cilia before and after airway cell culture
<p>High-speed video microscopy analysis (HSVA) is a diagnostic tool used within the UK Primary Ciliary Dyskinesia (PCD) Service to access airway ciliary function on nasal brushing biopsies. The Southampton PCD group is based at the University of Southampton, Faculty of Medicine and the University Hospital Southampton NHS Foundation Trust and is led by Professor Jane Lucas. We also use air-liquid interface (ALI) culture to differentiate airway epithelial cells to regrow healthy cilia to repeat standard PCD tests (including HSVA, immunofluoresence labelling of cilia proteins, transmission electron microscopy and functional genomics) and provide PCD research samples, which also allow us to develop new diagnostic approaches. ALI-culture can restore normal ciliary movement when secondary damage (due to infection or poor cell health) temporarily causes of abnormal cilia movement or a lack of cilia. ALI-culture can also re-confirm when ciliary defects and abnormal ciliary function are permanent and cause by inherited PCD (a ciliopathy). </p>
High-speed TIRF and 2D super-resolution structured illumination microscopy with large field of view based on fiber optic components
<p>Super-resolved structured illumination microscopy (SR-SIM) is among the most flexible, fast, and least perturbing fluorescence microscopy techniques capable of surpassing the optical diffraction limit. Current custom-built instruments are easily able to deliver two-fold resolution enhancement at video-rate frame rates, but the cost of the instruments is still relatively high, and the physical size of the instruments based on the implementation of their optics is still rather large. Here, we present our latest results towards realizing a new generation of compact, cost-efficient, and high-speed SR-SIM instruments. Tight integration of the fiber-based structured illumination microscope capable of multi-color 2D- and TIRF-SIM imaging, allows us to demonstrate SR-SIM with a field of view of up to 150 × 150 μm<sup>2</sup> and imaging rates of up to 44 Hz while maintaining highest spatiotemporal resolution of less than 100 nm. We discuss the overall integration of optics, electronics, and software that allowed us to achieve this, and then present the fiberSIM imaging capabilities by visualizing the intracellular structure of rat liver sinusoidal endothelial cells, in particular by resolving the structure of their trans-cellular nanopores called fenestrations.</p>
Data of "High-speed cryo-microscopy proves that ice-nucleating proteins of Pseudomonas syringae trigger freezing at hydrophobic interfaces"
<p>Raw data of a study titled <strong><em>"High-speed cryo-microscopy proves that ice-nucleating proteins of Pseudomonas syringae trigger freezing at hydrophobic interfaces"</em></strong>.</p> <p>The <strong>onset_locations.zip</strong> folder contains all analyzed images which are screenshots from the cryo-microscopic videos. Raw screenshots and evaluated images are included in two sub-folders per samples. The sample description is the name of the folders.</p> <p>The <strong>ice_propogation_velocity.zip</strong> folder contains all images that were used for the evaluation of the propagation velocity of ice. Every sample folder contains the original spot detection image, one image at a later time point, the subtracted image, and one image with the measured distance of the ice front indicated as white scale bar.</p> <p>The <strong>Results_(ice_propagation_velocity).xlsx</strong> contains the results from the velocity calculations, the <strong>Results_(surface tension).xlsx</strong> contains the evaluated surface tension values and the <strong>Results_(temperatures and locations).xlsx</strong> file contains all evaluated freezing locations (polar coordinates) and temperatures of all analyzed samples.</p>
Hyperspectral Multipoint High-Speed Confocal Microscopy Supporting Information Movies
<p><strong>S1 Movie. Neuronal growth in a zebrafish embryo</strong>. Sensory neurons (green) are labeled with GFP and motor neurons (magenta) are labelled with mCherry. The video was captured with filter configuration 2 (see section "System Dichroics and Emission Filters") frames were taken every minute.</p> <p> </p><p><strong>S2 Movie. Video of mitosis in a live <em>Xenopus laevis</em> embryo.</strong> The cellular membranes (magenta) is labelled with mTagBFP::CAAX; chromatin (blue) is labelled with miRFP670::H2B; and mitotic spindles (green) are labelled with mCherry:α-tubulin. One frame of 75 ms exposure time was captured every 5 seconds. The video was captured with filter configuration 2 (see section "System Dichroics and Emission Filters").</p> <p></p>
High-Speed Atomic Force Microscopy Highlights New Molecular Mechanism of Daptomycin Action
<p>Data underlying the figures in the publication “High-speed atomic force microscopy highlights new molecular mechanism of daptomycin action”, published in <em>Nat Commun, </em><strong>2020</strong>, 11, 6312. <a href="https://doi.org/10.1038/s41467-020-19710-z">https://doi.org/10.1038/s41467-020-19710-z</a></p> <p>Table of contents:</p> <p><strong>1.</strong> <strong>Movie 1</strong>; HS-AFM movie of the first minutes after exposure to sub-MIC Dap on a POPG supported membrane. Guides to the eye highlights those oligomers identifiable. Movie parameters: frame rate 33 ms; full image of 90 nm x 65 nm and 256x180 pixels; colour depth 8bit (256 values); full colour scale 4 nm.</p> <p><strong>2. </strong><strong>Movie 2</strong>; HS-AFM movie after tens of minutes after exposure to sub-MIC Dap that shows diffusing dimples on a POPG supported membrane which interact by swinging trajectories. Movie parameters: frame rate 83 ms; full image of 150nm x 150nm and 256x256 pixels; colour depth 8bit (256 values); full colour scale 4 nm.</p> <p><strong>3. </strong><strong>Movie 3</strong>; HS-AFM movie of the first minutes after exposure to over-MIC of a POPG supported membrane. A flow of material is visualized thanks to the motion of the ripples, it starts at the lm3m cubic phase (left) and ends at a tubulation (right). Movie parameters: frame rate 456 ms; full image of 400nm x 400nm and 300x300 pixels; colour depth 8bit (256 values); full colour scale 16 nm.</p> <p><strong>4. </strong><strong>Movie 4</strong>; HS-AFM movie of the cyclic accumulation of material in the pores created on TOCL/POPG supported membranes under the exposure of the outer leaflet to supplementary quantities of Dap added to the imaging solution. The process seems to eject material out of the membrane; see the material that appears next to the pore at 1.30s. Movie parameters: frame rate 260 ms; zoom of a full image of 140nm x 100nm and 256x180 pixels; colour 29 depth 8bit (256 values); full colour scale 3 nm.</p>
Pilot Study of Imaging Human Skin With High-Speed Spectrally Encoded Confocal Microscopy
ClinicalTrials.gov study NCT04566302. IPD Sharing: NO. Countries: 1. Publications: 0.
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