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A droplet digital polymerase chain reaction assay to detect rare helminth parasites infecting natural host populations (Vancouver Island 2023, University of Wisconsin Madison Laboratory colony 2024)
Helminth infections represent a significant challenge to human, livestock, and wildlife health, yet they remain relatively under-studied, especially in terms of their ecological impacts. Better understanding of how these parasites spread in wildlife populations could improve our ability to predict and manage disease transmission across various species. Traditional detection methods, such as visually identifying parasites in environmental samples or infected hosts, often fall short, especially during the early stages of infection when parasite loads are minimal. In this study, we introduce a highly sensitive and precise droplet digital PCR (ddPCR) assay that quantifies helminth DNA in aquatic habitats, focusing on the 18S rRNA gene as a marker. These data utilize the model host-parasite system between the tapeworm Schistocephalus solidus, and its cyclopoid copepod host, Acanthocyclops robustus. The molecular assays are built around creating an infection standard in the lab, where copepods were singly infected with a single tapeworm parasite. We extracted DNA from 100 infected adults and used this as a standard to translate gene copy numbers from the ddPCR reactions to actual animal values. After creating a known lab standard, we then use the generated probes and primers to detect (and quantify!) infection burdens in field samples, which include both water filter samples (eDNA) and zooplankton tows from several lakes around Vancouver Island, B.C. The data presented here include well-specific data from ddPCR runs (amplitude of individual level oil droplets in the reaction) as well as each ddPCR analysis in its entirety. In order to prove the specificity of probes and probe-primers, we include here ddPCR runs of closely related helminth species, Schistocephalus cotti and Schistocephalus pungitii. We also consider the binding to another genera of copepod, the calanoid Eurytomora. All of the data wrangling, analysis, and data visualization are included as .Rmd files in th
Occurrence Record Dataset from "Annotated checklist of the bees of Bonaire, with a focus on host plants"
<p>This is the occurrence dataset created for the publication "Annotated checklist of the bees of Bonaire, with a focus on host plants" (<a href="https://natuurtijdschriften.nl/pub/1026875" target="_blank" rel="noopener">https://natuurtijdschriften.nl/pub/1026875</a>).</p> <p>Observation and specimen data were assembled for this dataset, with the majority of records obtained during the Bonaire Estafette Expeditie (BEE). All citizen science records from Observation.org and iNaturalist.org up to December 2023 have been critically reviewed.<br>A project was created (<a href="https://www.inaturalist.org/projects/flower-visitors-and-pollinators-of-the-caribbean" target="_blank" rel="noopener">Flower visitors and pollinators of the Caribbean</a>) to improve standardized data collecting of plant-pollinator interactions and on <a href="https://observation.org/">observation.org</a> the standardized fields for interactions were used.<br>Records from passive trapping methods are not included. All bees were either observed or collected by hand or insect net. The majority of specimens will be accessible in the collection of Naturalis Biodiversity Center (RMNH), Leiden (the Netherlands). A synoptic collection is retained at the University of Tartu Zoological Collections in Tartu, Estonia (TUZ).</p> <p>The occurrence dataset (Version 1.4 and later) is:</p> <ul> <li>conform Darwin Core (DwC): <a href="https://dwc.tdwg.org/terms/">https://dwc.tdwg.org/terms</a></li> <li>in the data format CSV (tab delimited values) and UTF-8 encoded</li> </ul> <p> </p> <p><strong>DwC terms (Column labels) used in the dataset with their description:</strong></p> <table> <tbody> <tr> <td><strong>Column label</strong></td> <td><strong>Column description</strong></td> </tr> <tr> <td>occurrenceID</td> <td>Unique identifier or URI (GUID) for each record, mainly unique URLs generated by the web-based data holder.</td> </tr> <tr> <td>catalogNumber</td> <td>Unique code derived from URI in occurrenceID. Each specimen bears a label with this identifier and multimedia are tagged with this identifier.</td> </tr> <tr> <td>recordNumber</td> <td>Sample field ID used to manage data of preserved specimen occurrence records.</td> </tr> <tr> <td>otherCatalogNumbers</td> <td>Other unique identifiers used on specimen labels, but not derived from an URI.</td> </tr> <tr> <td>scientificName</td> <td>The scientific name of the lowest taxonomic rank to which the individual(s) was identified.</td> </tr> <tr> <td>scientificNameAuthorship</td> <td>The author name and year of publication in accordance with ICZN rules.</td> </tr> <tr> <td>verbatimIdentification</td> <td>The original identification, including qualifiers if needed.</td> </tr> <tr> <td>individualCount</td> <td>The number of individuals present at the time of the occurrence.</td> </tr> <tr> <td>sex</td> <td>The sex of the individual(s). The values female, male or unknown are used, if a mixed group is observed multiple values are listed.</td> </tr> <tr> <td>lifeStage</td> <td>The life stage of the individual(s).</td> </tr> <tr> <td>basisOfRecord</td> <td>The specific nature of the data record at the time of the identification (e.g. PreservedSpecimen).</td> </tr> <tr> <td>identifiedBy</td> <td>The name of the person who made the identification in the field or based on collected evidence (e.g. specimen or photo).</td> </tr> <tr> <td>identificationQualifier</td> <td>In case the identification could be given only to a species group 'cf.' is recorded.</td> </tr> <tr> <td>dateIdentified</td> <td>The year when the identification was made.</td> </tr> <tr> <td>previousIdentifications</td> <td>The scientific name originally given to the observed or collected individual(s).</td> </tr> <tr> <td>order</td> <td>The name of the order (e.g. Hymenoptera).</td> </tr> <tr> <td>family</td> <td>The name of the family (e.g. Apidae).</td> </tr> <tr> <td>genus</td> <td>The name of the genus (e.g. Apis).</td> </tr> <tr> <td>subgenus</td> <td>The name of the subgenus (e.g. Apis).</td> </tr> <tr> <td>specificEpithet</td> <td>The name of the species, epithet as given in dwc:scientificName.</td> </tr> <tr> <td>taxonRank</td> <td>The taxonomic rank of the most specific name in dwc:scientificName.</td> </tr> <tr> <td>eventDate</td> <td>The date-time when the event was observed and recorded. The event date uses the ISO 8601-1:2019 standard, with the following formatting being used: format YYYY-MM-DD, or YYYY if only the year is known. If time of capture is known, then format is YYYY-MM-DDTHH:MM, with HH:MM the local time.</td> </tr> <tr> <td>year</td> <td>The year in which the event was observed and recorded.</td> </tr> <tr> <td>month</td> <td>The month in which the event was observed and recorded.</td> </tr> <tr> <td>day</td> <td>The day in which the event was observed and recorded.</td> </tr> <tr> <td>eventTime</td> <td>The time or interval during which the event occurred.</td> </tr> <tr> <td>samplingProtocol</td> <td>The name or description of the collecting or recording method used.</td> </tr> <tr> <td>behavior</td> <td>A description of the behavior shown by the individual(s) recorded in this occurrence.</td> </tr> <tr> <td>decimalLatitude</td> <td>The geographic latitude in decimal degrees recorded by a GPS device (WGS84) when observing and recording the occurrence.</td> </tr> <tr> <td>decimalLongitude</td> <td>The geographic longitude in decimal degrees recorded by a GPS device (WGS84) when observing and recording the occurrence.</td> </tr> <tr> <td>geodeticDatum</td> <td>The ellipsoid, geodetic datum, or spatial reference system (SRS) upon which the geographic coordinates given in dwc:decimalLatitude and dwc:decimalLongitude is based.</td> </tr> <tr> <td>verbatimLocality</td> <td>The original textual description of the place.</td> </tr> <tr> <td>island</td> <td>The name of the island.</td> </tr> <tr> <td>countryCode</td> <td>The standard ISO 3166-1 alpha-2 country code for the country.</td> </tr> <tr> <td>coordinateUncertaintyInMeters</td> <td> <p>The horizontal distance (in meters) from the given dwc:decimalLatitude and dwc:decimalLongitude describing the smallest circle containing the actual location, usually the EPE (Estimated Position Error) from the GPS device. The EPE is here measured as the horizontal position error in meters.</p> </td> </tr> <tr> <td>recordedBy</td> <td>A person, group, or organization observing and recording the occurrence.</td> </tr> <tr> <td>associatedTaxa</td> <td>The type of association and the scientific name of the host taxon is recorded that is associated/has relationship with the taxon in dwc:scientificName. The association/relationship is recorded using the format as in the following example: "floral host":"Lantana sp."</td> </tr> <tr> <td>occurrenceRemarks</td> <td>Comments or notes about the dwc:Occurrence.</td> </tr> <tr> <td>associatedSequences</td> <td>A list (concatenated and separated) of identifiers (publication, global unique identifier, URI) of genetic sequence information.</td> </tr> <tr> <td>typeStatus</td> <td>A list (concatenated and separated) of nomenclatural types (type status, typified scientific name, publication) applied to the subject.</td> </tr> <tr> <td>collectionCode</td> <td>The name, acronym, coden, or initialism identifying the collection or data set from which the record was derived.</td> </tr> <tr> <td>identificationRemarks</td> <td>Comments or notes about the identification.</td> </tr> <tr> <td>identificationReferences</td> <td>A reference or list of references (publication, global unique identifier, URI) used for the identification.</td> </tr> <tr> <td>nameAccordingTo</td> <td>A reference to the checklist or publication that was followed to record the name in dwc:scientificName.</td> </tr> <tr> <td>samplingEffort</td> <td>The amount of effort, expressed in minutes or hours, to obtain and record the occurrences.</td> </tr> <tr> <td>occurrenceStatus</td> <td>A statement about the presence or absence of a taxon during the time of an event.</td> </tr> <tr> <td>disposition</td> <td>The current state of a specimen with respect to a collection.</td> </tr> <tr> <td>language</td> <td>The language of the record using ISO 639-1 codes, e.g. en</td> </tr> </tbody> </table>
Effects of factorial nitrogen, phosphorus, and potassium with micronutrient addition and Host Community on Fungal Endophyte Diversity at Cedar Creek Ecosystem Reserve, Minnesota, USA, 2014
The microbes contained within free-living organisms can alter host growth, reproduction, and interactions with the environment. In turn, processes occurring at larger scales determine the local biotic and abiotic environment of each host that may affect the diversity and composition of the microbiome community. Here, we examine variation in the diversity and composition of the foliar fungal microbiome in the grass host, Andropogon gerardii, across a factorial nitrogen, phosphorus, and potassium addition experiment in Minnesota, USA. We found limited evidence of direct effects of nutrients on endophyte diversity. Instead, the effects of nutrients on endophyte diversity appeared to be mediated by accumulation of plant litter and plant diversity loss. Specifically, nitrogen addition is associated with a 40% decrease in plant diversity and an 11% decrease in endophyte richness. Although nitrogen, phosphorus, and potassium addition increased aboveground live biomass and decreased relative Andropogon cover, endophyte diversity did not covary with live plant biomass or Andropogon cover. Our results suggest that fungal endophyte diversity within this focal host is determined in part by the diversity of the surrounding plant community and its potential impact on immigrant propagules and dispersal dynamics. Our results suggest that elemental nutrients reduce endophyte diversity indirectly via impacts on the local plant community, not direct response to nutrient addition.
Diffraction images used to solve the structures published in the article "Structure of human endo-α-1,2-mannosidase (MANEA), an antiviral host-glycosylation target"
<p>Raw diffraction images used for generating the structures published in the article "Structure of human endo-α-1,2-mannosidase (MANEA), an antiviral host-glycosylation target" (available <a href="https://doi.org/10.1073/pnas.2013620117">here</a>). Full single-crystal datasets, including images that were not used in the final analyses, are published. The software used for the processing of each dataset is listed in their respective PDB entries. Datasets 6ZJ1 and 6ZJ5 were cut anisotropically using STARANISO, other datasets were processed isotropically.</p> <p> </p> <p>If you find this useful, please contact me at <a href="mailto:lukasz.sobala@hirszfeld.pl">lukasz.sobala@hirszfeld.pl</a>, I am just interested in how these data are used!</p>
Data from: Spatial and host-related variation in prevalence and population density of wheat curl mite (Aceria tosichella) cryptic genotypes in agricultural landscapes
<p><strong>Filename: coord.csv</strong></p> <p>Names of the sampling locations and their geographic coordinates.</p> <ol> <li>Name - sampling locality identifier</li> <li>Lat - latitude</li> <li>Long - longitude</li> </ol> <p> </p> <p><strong>Filename: lineages.csv</strong></p> <ol> <li>id.sample - sample identifier</li> <li>host - host species (Arrela=<em>Arrhenantherum elatius</em>, Avesat=<em>Avena sativa</em>, Broine=<em>Bromus inermis</em>, Elyres=<em>Elymus repens</em>, Horvul=<em>Hordeum vulgaris</em>, Seccer=<em>Secale cereale</em>, Triaes=<em>Triticum aestivum</em>, Tririm=<em>Triticale rimpaui</em></li> <li>x, y - geodetic coordinates</li> <li>stems - no. of stems in a sample</li> <li>leaves - no. of leaves in a sample</li> <li>MT.01 to MT.27 - no. of mites belonging to each genetic lineage</li> </ol>
The Pathogen-Host Interactions Database, version 4.18
<p>PHI-base is an online database (available at <a href="http://www.phi-base.org">phi-base.org</a>) that catalogues experimentally verified pathogenicity, virulence and effector genes from fungal, oomycete and bacterial pathogens, which infect animal, plant, fungal and insect hosts. PHI-base is a valuable resource in the discovery of genes in medically and agronomically important pathogens, which may be potential targets for chemical intervention.</p> <p>Each entry in PHI-base is curated by domain experts and is supported by strong experimental evidence (for example, gene disruption and gene complementation experiments), as well as literature references in which the original experiments are described. Each gene in PHI-base is presented with its nucleotide sequence and deduced amino acid sequence (available in a FASTA file), as well as a detailed description of the predicted protein's function during the host infection process. To facilitate data interoperability, we have annotated genes using ontologies, controlled vocabularies, and links to external sources (including UniProt, Gene Ontology, Enzyme Commission, NCBI Taxonomy, EMBL, PubMed and FRAC).</p> <p>This PHI-base dataset is a Frictionless Data Package that contains an export of the PHI-base database in CSV format (comma-separated values), plus a FASTA file with sequences for each gene in the database. This version of the dataset, version 4.18, contains 5,828 publications, covering 23,497 pathogen–host interactions and 10,614 pathogen genes across 335 pathogen species and 265 host species.</p>
(Fastq Files) Amplicon sequencing of ama1 and mdr1 to track within-host P. falciparum diversity in Kilifi, KENYA
<p>These data were generated from amplicon sequencing of <em>Plasmodium falciparum</em> <em>ama1 </em>and<em> </em><em>mdr1</em> genes in samples collected from Kilifi, at the coast of Kenya.</p> <p>The two papers that reference these data will soon be included here:</p> <ol> <li> The Journal of Infectious Diseases - https://doi.org/10.1093/infdis/jiac144</li> <li>Wellcome Open Research - https://wellcomeopenresearch.org/articles/7-95</li> </ol> <p>Two objectives were explored:</p> <ol> <li>To determine temporal changes in the genetic diversity of malaria parasites in asymptomatic and febrile infections.</li> <li>To track within-host parasite diversity, throughout treatment in a clinical drug trial.</li> </ol>
Explosive networking: the role of adaptive host radiations and ecological opportunity in a species-rich host-parasite assembly
<p>Dataset for Cruz-Laufer et al. (2021) Explosive networking: the role of adaptive host radiations and ecological opportunity in a species-rich host-parasite assembly.</p> <p><strong>Abstract: </strong>Many species-rich ecological communities emerge from adaptive radiation events. The effects of this explosive speciation on community assembly remain poorly understood. Here, we explore the well-documented radiations of African cichlid fishes and their interactions with the flatworm gill parasites <em>Cichlidogyrus </em>spp., including 10529 reported infections and 477 different host-parasite combinations collected through a survey of peer-reviewed literature. We assess how evolutionary, ecological, and morphological parameters determine host-parasite meta-communities affected by adaptive radiation events through network metrics, host repertoire measures, and network link prediction. The hosts’ evolutionary history mostly determined host repertoires of the parasites. Ecological and evolutionary parameters determined host-parasite interactions. Generally, ecological opportunity and fitting have shaped cichlid-<em>Cichlidogyrus</em> meta-communities suggesting an invasive potential for hosts used in aquaculture. Meta-communities affected by adaptive radiations are increasingly specialised with higher environmental stability. These trends should be verified across other systems to infer generalities in the evolution of species-rich host-parasite networks.</p>
Responsive Environmental Assessment Commercially Hosted (REACH)
<p>This is the historical REACH data release. These are the “v3” files produced at The Aerospace Corporation, spanning March 2017 until December 2019. It is accompanied by a README (PDF), which includes a brief mission description, describes the features of the collection of dosimeters, discusses data availability throughout the dataset, describes data quality flags, and concludes with a data dictionary.</p>
Accompanying data for the PhD thesis 'Nanomaterial safety for microbially-colonized hosts'
<p><strong>These files include all data presented in chapter 6 of the dissertation:</strong></p> <p><strong>"Nanomaterial safety for microbially-colonized hosts: microbiota-mediated physisorption interactions and particle-specific toxicity" by Bregje Brinkmann (2022).</strong></p> <p>The data presented in chapters 2-5 have previously been published elsewhere:</p> <ul> <li>Chapter 2: <em>Zenodo</em> (DOI: <a href="https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2F10.5281%2Fzenodo.6800734&data=05%7C01%7Cb.w.brinkmann%40cml.leidenuniv.nl%7Cb5837fcc5a1b4cb5e88008da7615fe87%7Cca2a7f76dbd74ec091086b3d524fb7c8%7C0%7C0%7C637952134073527979%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=z4LB7Ziyiy%2BLXe0SllX67AJ%2F9zhfARBXp8QNzZsVg%2B4%3D&reserved=0">10.5281/zenodo.6800734</a>).</li> <li>Chapter 3: <em>Mendeley</em> <em>Data</em> (DOI: <a href="https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2F10.17632%2F2d4hcr5cb5.1&data=05%7C01%7Cb.w.brinkmann%40cml.leidenuniv.nl%7Cb5837fcc5a1b4cb5e88008da7615fe87%7Cca2a7f76dbd74ec091086b3d524fb7c8%7C0%7C0%7C637952134073527979%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=x7LFz2OgiJ20%2BD18QlwR9qIwGH%2BCbju4BKqkUaqIoXs%3D&reserved=0">10.17632/2d4hcr5cb5.1</a>)</li> <li>Chapter 4: <em>Figshare</em> (DOI: <a href="https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2F10.6084%2Fm9.figshare.c.4923261&data=05%7C01%7Cb.w.brinkmann%40cml.leidenuniv.nl%7Cb5837fcc5a1b4cb5e88008da7615fe87%7Cca2a7f76dbd74ec091086b3d524fb7c8%7C0%7C0%7C637952134073527979%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=n3wegOuHzKihCO%2FKbZhClnYHPyxWI0cALApBgLkUHnQ%3D&reserved=0">10.6084/m9.figshare.c.4923261</a>)</li> <li>Chapter 5: <em>Mendeley Data </em>(DOI: <a href="https://eur03.safelinks.protection.outlook.com/?url=https%3A%2F%2Fdoi.org%2F10.17632%2F4nfg69v8hy.1&data=05%7C01%7Cb.w.brinkmann%40cml.leidenuniv.nl%7Cb5837fcc5a1b4cb5e88008da7615fe87%7Cca2a7f76dbd74ec091086b3d524fb7c8%7C0%7C0%7C637952134073527979%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=0A0ktYDmgfxwc7U%2BgzHzQUj8Q5wixi%2BUzoSzMctlbso%3D&reserved=0">10.17632/4nfg69v8hy.1</a>)</li> </ul> <p><br> <strong>1. Data presented in Figure 6.1:</strong> Survival_CFU_(...)<br> Tab-delimited file with zebrafish larvae survival, and the number of colony-forming units (CFUs) associated with zebrafish larvae, following exposure to silver nanoparticles (nAg) from 3-5 days-post fertilization (dpf):</p> <ul> <li><em>Concentration</em>: Nominal exposure concentration (mg nAg·L<sup>-1</sup>).</li> <li><em>Date</em>: The date at which the mortality was scored (Format: DD/MM/YYYY). </li> <li><em>Family</em>: A code referring to the aquarium of wildtype zebrafish (ABxTL) that were crossed to obtain the larvae for the experiment. </li> <li><em>Survival</em>: Percentage of larvae that had survived the treatment.</li> <li><em>CFU</em>: Number of colony-forming units that was isolated per larva</li> </ul> <p>The methodology for toxicity tests and the procedures to determine CFU counts, have been published in <em>Nanotoxicology</em>:</p> <p>Brinkmann BW, Koch BEV, Spaink HP, Peijnenburg WJGM, Vijver MG. 2020. Colonizing microbiota protect zebrafish larvae against silver nanoparticle toxicity. Nanotoxicology. 14: 725-739. DOI: <a href="http://doi.org/10.1080/17435390.2020.1755469">10.1080/17435390.2020.1755469</a></p> <p> </p> <p><strong>2. Data presented in Figure 6.2:</strong> ABs_DoseResponses_(...)<br> Tab-delimited file with zebrafish larvae mortality following a pretreatment of 0, 6 or 72 hours with an antibiotic and antifungal cocktail, and subsequent exposure to nAg from 3-5 dpf:</p> <ul> <li><em>Concentration</em>: Nominal exposure concentration (mg nAg·L<sup>-1</sup>). </li> <li><em>Mortality</em>: Percentage of larvae that had died.</li> <li><em>Date</em>: The date at which the mortality was scored (Format: DD/MM/YYYY).</li> <li><em>Family</em>: A code referring to the aquarium of wildtype zebrafish (ABxTL) that were crossed to obtain the larvae for the experiment. </li> <li><em>ABs</em>: Duration of the antibiotic/ antifungal pretreatment, either 0, 6 or 72 hours.</li> </ul> <p> </p> <p><strong>3. Data presented in Figure 6.3:</strong> il1beta_eGFP_(...)<br> Three folders comprising fluorescence microscopy images (TIFF format) of il1beta:eGFP reporter zebrafish larvae at 5 dpf:</p> <ul> <li><em>(...)_replicates1_20200226</em>: images for the first experimental replicate.</li> <li><em>(...)_replicates2_20200304</em>: images for the second experimental replicate.</li> <li><em>(...)_replicates3_20200318</em>: images for the third experimental replicate.</li> </ul> <p>For each of the replicates, the following images were acquired:</p> <ul> <li><em>nZnO_GFP</em>: GFP signal for larvae exposed to nZnO.</li> <li><em>Znion_GFP</em>: GFP signal for larvae exposed to zinc ions.</li> <li><em>nZnO_trans</em>: transmitted light images for larvae exposed to nZnO. </li> <li><em>Znion_trans</em>: transmitted light images for larvae exposed to zinc ions.</li> </ul> <p>Additionally, the following images have previously been deposited to <em>Mendeley Data </em>(DOI: <a href="http://doi.org/10.1016/j.ecoenv.2022.113522">10.17632/4nfg69v8hy.1</a>):</p> <ul> <li><em>nAg_GFP</em>: GFP signal for larvae exposed to nAg.</li> <li><em>nAg_trans</em>: transmitted light images for larvae exposed to nAg.</li> <li><em>Agion_GFP</em>: GFP signal for larvae exposed to silver ions.</li> <li><em>Agion_trans</em>: transmitted light images for larvae exposed to silver ions.</li> <li><em>control_GFP</em>: GFP signal for control larvae that had not been exposed to silver ions or nAg</li> <li><em>control_trans</em>: transmitted light images for control larvae that had not been exposed to silver ions or nAg.</li> </ul> <p>All image processing steps have been published in <em>Ecotoxicology and Environmental Safety</em>:</p> <p>Brinkmann BW, Koch BEV, Peijnenburg WJGM, Vijver MG. 2022. Microbiota-dependent TLR2 signaling reduces silver nanoparticle toxicity to zebrafish larvae. Ecotox Environ Saf. 237: 113522. DOI: <a href="http://doi.org/10.1016/j.ecoenv.2022.113522">10.1016/j.ecoenv.2022.113522</a></p> <p> </p> <p><strong>Abbreviations:</strong></p> <ul> <li><em>ABs</em>: antibiotics</li> <li><em>CFU</em>: colony-forming units</li> <li><em>dpf</em>: days post-fertilization</li> <li><em>il1beta</em>: interleukin-1beta</li> <li><em>nAg</em>: silver nanoparticles (NM-300 K)</li> <li><em>nZnO</em>: zinc oxide nanoparticles (NM-110)</li> </ul>
Manipulating a host-native microbial strain compensates for low microbial diversity by increasing weight gain in a wild bird population
<h1>Manipulating a host-native microbial strain compensates for low microbial diversity by increasing weight gain in a wild bird population</h1> <h1> </h1> <p>These files contain data on bacteria present in the guts of wild great tit (Parus major) obtained from faecal samples and sequenced using Illumina MiSeq. These data resulted from an experiment which provided supplementary mealworms at the nest during the breeding season at number of woodland sites in Cork, Ireland. Approximately half of these nests were given mealworms covered in a freeze dried bacterial powder containing the bacteria Lactobacillus kimchicus, which had been isolated from great tit faeces from the previous season. This treatment aimed to disrupt the gut microbiota of the treatment birds in order to provide evidence for the gut microbiotas role in birds health and fitness. Included here are the 3 elements necessary to create a 'phyloseq object' containing the sample metadata, ASV (Amplicon Sequence Variant) count table and a taxonomy table. The metadata file includes the alpha diversity scores for each individual. The data include all negative control samples taken during sample collection and library preparation, which were removed before the main analyses. All analyses, except for the beta-diversity analyses, were conducted in R. All R code is available on GitHub (https://github.com/shan-e-s\). Raw Sequence data are available in the European Nucleotide Archive under access number PRJEB74941, and ERS18960426-ERS18960697.</p> <h2> </h2> <h2>## Description of the data and file structure </h2> <p>Taxonomy, ASV and metadata files required to create a phyloseq object in R. metadata.csv file contains data on individual birds (i.e. individual samples). The metadata includes descriptions of the bird itself and it's environment, namely:</p> <ul> <li>Rownames: unique sample ID for each sample, corresponds with asvTable.csv. </li> <li>Nest: unique identifier for the nest box associated with the bird being sampled. </li> <li>Sample.ID: unique identifier for the faecal sample or control sample.</li> <li>Bird.ID: Identity of the bird the sample came from, note some individuals sampled twice so some bird.ID's may reoccur in metadata with different Sample.ID.</li> <li>Date: Date the sample was taken dd/mm/yyyy.</li> <li>Day: Date the sample was taken, in days since 1st March.</li> <li>Ring.Mark: British Trust for Ornithology (BTO) metal ring ID where applicable. Birds only ringed at D15 so some young birds do not have IDRings.</li> <li>Site: ID of woodland site that bird was sampled at.</li> <li>Chick.LetterID: ID letter differentiates between different birds from the same nest. Either 'A'-'F' for nestlings, 'Fe' for females or 'M' for males.</li> <li>Age.code: BTO age code.</li> <li>Age.category: Age category that bird is in. D8 = 8 days post hatching, D15 = 15 days post hatching, adult = 1+ years post hatching.</li> <li>Sex: Bird's sex, only determined for adult birds. Fe = Female, M = Male.</li> <li>Wing_mm: Wing length in mm.</li> <li>Tarsus_mm: minimum tarsus length of bird in mm.</li> <li>Weight_g: bird's weight in grams.</li> <li>Faecal.Sample: bird's age at sampling.</li> <li>newRing: whether bird was fitted with a new BTO ring. Only relevant to adults.</li> <li>Treatment: the experimental treatment group that the bird was in. Either 'Treatment' when nest given L. kimchicus treated mealworms or 'Control' when nest given plain mealworms.</li> <li>Notes: field notes.</li> <li>Main.sample: indicates whether this sample was the main sample to be used for analysis, an alternative sample taken as a backup.</li> <li>Plate: the ID of the PCR plate which the sample was amplified on.</li> <li>Azenta_noPeriod: sample ID given to sequencing facility without special characters. Corresponds to fastq files and ASV table counts.</li> <li>Qubit_prePool: samples qubit score before pooling.</li> <li>Date_extracted: date the sample was extracted on dd/mm/yyyy.</li> <li>SampleType: whehther the sample was a 'main' sample intended for downstream analysis, a 'control' sample for detecting contamination during library preparation, a 'duplicate' for detecting PCR issues, a 'label_error' where sample was suspected of being mislabelled at some point, a 'repeat' sample intended to detect errors or issues, a 'contam' sample which was suspected of being contaminated, a 'common' sample used across different PCR plates to detect issues. Extraction_notes: notes regarding the DNA extraction of the sample. </li> <li>LibPrep_notes: notes regarding the library preparation of the sample.</li> <li>Ring.Mark.lab: the ring or sample ID written on the sample tube, recorded to help detect mislabelling.</li> <li>Post_lab_notes: notes regarding issues found post sequencing.</li> <li>NumberOfReads: number of sequence reads associated with the sample. </li> <li>DistanceToEdge: distance between nest and woodland edge in metres. </li> <li>BroodSize.D8: number of nestlings in the nest at day-8 post hatching. </li> <li>BroodSize.D15: number of nestlings in the nest at day-15 post hatching.</li> <li>firstEggLayDate: Date the first egg in the clutch was laid, in days since 1st March.</li> <li>lastEggLayDate: Date the last egg in the clutch was laid, in days since 1st March.</li> <li>Observed: number of unique ASV's (or taxa) detected in the sample.</li> <li>Chao1: Chao1 diversity of the sample.</li> <li>Shannon: Shannon diversity of the sample.</li> </ul> <p>The file 'taxonomy.csv' contains the taxonomic breakdown of each bacterial Amplicon Sequence Variant (ASV) found in the dataset from Phylum to Species. Obtained by using the Naive Bayes Classifier against the Silva (v138) taxonomic database.</p> <p>The file 'asvTable.csv' contains counts of each amplicon sequence variant's occurrence for each individual sample. Samples are rows and taxa are columns.</p> <p> </p> <h2>Sharing/Access information </h2> <p>All R code is available on GitHub (https://github.com/shan-e-s\). Raw Sequence data are available in the European Nucleotide Archive under access number PRJEB74941, and ERS18960426-ERS18960697.</p>
Metabarcoding reveals a high diversity of woody host-associated Phytophthora spp. in soils at public gardens and amenity woodlands in Britain
<p>This is the demultiplexed Illumina MiSeq raw sequencing data from two 96-well plates from the following recent publication, shared with permission of the corresponding author, Sarah Green:</p> <p>Riddell <em>et al.</em> (2019). Metabarcoding reveals a high diversity of woody host-associated <em>Phytophthora</em> spp. in soils at public gardens and amenity woodlands in Britain. https://doi.org/10.7717/peerj.6931<br> <br> It consists of 244 gzipped compressed plain text FASTQ format sequence files, grouped into 122 pairs by the widely used R1 and R2 suffix. The files have been renamed to use the anonymised site numbers (1 to 14) as in the paper, see also supplementary table one for site metadata. Additionally there are two negative controls, and positive control DNA mixtures of 10 and 15 species as described in the paper.<br> </p>
Dietary fibers boost gut microbiome-produced B vitamin pool and alter host immune landscape
<p>This dataset contains fcs files of lymphocytes from the colonic lamina propria, lungs, and spleens of specific-pathogen-free (SPF), gnotobiotic (14-member synthetic microbiota, 14SM) or germ-free (GF) mice fed five distinct rodent diets (Standard chow 1, SC1; Standard chow 2, SC2; Fiber-supplemented diet, FS; Inulin-supplemented diet, IN; or Fiber-free diet, FF), analysed by mass cytometry. Three million cells per organ per animal were transferred into 15 mL conical tubes. For live/dead staining, cells were incubated with 5 μM cisplatin for 5 minutes. Cells were washed, and cell surface staining mix was added containing pre-conjugated antibodies for 30 minutes at room temperature. Samples were washed twice with FACS buffer, then fixed using the FoxP3 Fix/Perm kit (eBiosciences) for 45 minutes at 4°C, followed by permeabilization wash. Samples were then incubated with the intracellular staining mix for 30 minutes at room temperature. Cells were washed with FACS buffer twice, and pellets were resuspended in Cell-ID™ Intercalator-Ir (Fluidigm) in MaxPar fixation solution (Fluidigm, catalogue no. 201192B) and refrigerated overnight, or for up to five days. Prior to acquisition, samples were washed twice with 1X PBS, and then washed twice with deionized water. Cell pellets were further resuspended in deionized water at 0.5 × 10^6 cells/mL and topped up with 10% calibration beads (EQ Four Element Calibration Beads, Fluidigm). All samples were acquired on the Helios Mass Cytometer (Fluidigm). Effector immune populations and activated T cells in the gut accumulate in a microbiota-dependent manner. Shifts in the microbiome according to dietary fiber source and content result in altered concentrations of B vitamins available to the host, which is tied to distinct alterations in innate and adaptive immune populations. </p>
Composite X-EUV + optical model spectrum of the planet-hosting star HIP 67522 (HD 120411)
<p>Composite spectrum of HIP 67522 obtained by joining a Phoenix photospheric spectrum with the X-EUV spectrum synthesized from the reconstructed plasma Emission Measure Distribution (EMD) vs. temperature in chromosphere, transition region, and corona. The FITS file contains 3 extensions with the spectrum, the EMD, and the plasma chemical abundances, derived from the analysis of X-ray and FUV high-resolution spectra, obtained with simultaneous observations with XMM-Newton and HST.</p> <p>In the attached figure, the upper panel shows the specific flux at Earth, while the bottom panel is the photon flux at a distance of 1 AU. In green the Phoenix spectrum resampled to a wavelength resolution of 1 Angstrom, down to 1700 A; the XUV spectrum in the range 1-1700 A instead has a resolution of 0.01 A. The green and blue segments in the upper panel, at about 200 nm, mark the Phoenix model flux and the observed flux integrated over the OM UVM2 band.</p>
Graphic Illustration of Kelly Speer's Keynote Talk: Hosts, parasites, and microbiomes: A system for studying natural complexity in a changing world
<p><a href="https://lib.ku.edu/people/courtney-foat" target="_blank" rel="noopener">Courtney Foat</a>, Advisor for Strategic Initiatives & Organizational Engagement at the University of Kansas, graphically recorded and synthesized the Keynote Talk by Kelly Speer at the Digital Data 2024 Conference in Lawrence, Kansas in May of 2024. We include this resource, with permission, because of its relevance to our NSF-supported Workshop: Digital Collections Data and Tracking Disease.</p>
Graphic Illustration of Kendra Phelp's Talk: A harmonized taxonomic resource is critical for accurately interpreting host-pathogen interactions
<p><a href="https://lib.ku.edu/people/courtney-foat" target="_blank" rel="noopener">Courtney Foat</a>, Advisor for Strategic Initiatives & Organizational Engagement at the University of Kansas, graphically recorded this invited talk by Kendra Phelps at an NSF-supported Workshop: Digital Collections Data and Tracking Disease.</p>
Datasets and R source code of manuscript "Parasites make hosts more profitable but less available to predators"
<p>Data about experimentations of DIV-1 (virus) infection on Daphnia magna.</p> <p>Linked article: Parasites make hosts more profitable but less available to predators</p>
CSM08 Small mammal host-parasite sampling data for 16 linear trapping transects located in 8 LTER burn treatment watersheds at Konza Prairie
Data set contains summaries (summer) of the number of individuals of each species of small mammal captured (relative abundance) on each transect. Each record contains date, treatment, transect, trap station, species, specimen number, recapture status, specimen disposition, external body measurements (where applicable), reproductive information, and miscellaneous associated comments. These sampling records are based on nightly captures during one 4-night trapping period in summer (June through August) for each of 16 permanent transects established on eight fire treatments (two transects per treatment). These treatments include two seasonal burn watersheds (SpB, SuB), two reversal burn watersheds (R1A, R20A), one annual burn watershed (1D), two 4-year burn watersheds (4B, 4F, and one 20-year burn watershed (20B). None of these treatments implement bison grazing.
Virgina Coast Reserve LTER Externally-hosted Models and Data
This dataset contains a listing of models and data associated with Virginia Coast Reserve Long-Term Ecological Research that are not otherwise available in the VCR/LTER data archive. It includes links to models and data that have been made available by researchers that are not in the VCR/LTER archive because they are deposited in a specialized system specifically tailored to a specific type (e.g. models) or in another archive due to requirements and constraints imposed by funders and journals. It takes the form of a spreadsheet including for each item the type (i.e., model or data), Internet link, name, brief description and optionally a link to a publication that used the data.
Exploring Data Evaluation Strategies for Enhanced Identification of Host Cell Proteins in Drug Products of Therapeutic Antibodies and Fc-Fusion Proteins
<p>This data repository contains all previously unpublished raw data files for the manuscript “Exploring Data Evaluation Strategies for Enhanced Identification of Host Cell Proteins in Drug Products of Therapeutic Antibodies and Fc-Fusion Proteins” by Wolfgang Esser-Skala, Marius Segl, Therese Wohlschlager, Veronika Reisinger, Johann Holzmann, and Christian G. Huber. See <em>readme.md</em> for further information.</p>
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.