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16 results for “hybrid male sterility”
Data from: Genetic dissection of hybrid male sterility across stages of spermatogenesis
Hybrid sterility is a common form of reproductive isolation between nascent species. Although hybrid sterility is routinely documented and genetically dissected in speciation studies, its developmental basis is rarely examined, especially in generations beyond the F1. To identify phenotypic and genetic determinants of hybrid male sterility from a developmental perspective, we characterized testis histology in 312 F2 hybrids generated by intercrossing inbred strains of Mus musculus domesticus and M. m. musculus, two subspecies of house mice. Hybrids display a range of histologic abnormalities that indicate defective spermatogenesis. Among these abnormalities, we quantified decreased testis size, reductions in spermatocyte and spermatid number, increased apoptosis of meiosis I spermatocytes, and more multinucleated syncytia. Collectively, our phenotypic data point to defects in meiosis I as a primary barrier to reproduction. We identified seven quantitative trait loci (QTL) controlling five histologic traits. A region of chromosome 17 that contains Prdm9, a gene known to confer F1 hybrid male sterility, affects multinucleated syncytia and round spermatids, potentially extending the phenotypic outcomes of this incompatibility. The X chromosome also plays a key role, with loci affecting multinucleated syncytia, apoptosis of round spermatids, and round spermatid numbers. We detected an epistatic interaction between QTL on chromosomes 17 and X for multinucleated syncytia. Our results refine the developmental basis of a key reproductive barrier in a classic model system for speciation genetics.
Six genome assemblies of Drosophila species for: Identification and genetic analysis of a pervasive "needle-eye" sperm phenotype in Drosophila sterile hybrid males
<p>Interspecies hybrid sterility has been extensively studied, especially in the genus <em>Drosophila</em>. Hybrid sterility is more often found in the heterogametic (XX or ZW) sex, a trend called Haldane's rule. Although this phenomenon is pervasive, identification of a common genetic mechanism remains elusive, with modest support found for a range of potential theories. Here, we identify a single precise morphological phenotype, which we call "needle-eye sperm," that is associated with hybrid sterility in three separate species pairs that span the <em>Drosophila</em> genus. The nature of the phenotype indicates a common point of meiotic failure in sterile hybrid males. We used ten generations of backcross selection paired with whole-genome pooled sequencing to genetically map the regions underlying the needle-eye sperm phenotype. Surprisingly, the sterility phenotype was present in ~50% of males even after ten generations of backcrossing, yet the genetic map showed multiple regions associated with sterility, indicating multiple regions may have the capacity to be sufficient to induce sterility in the F1. Due to the common phenotype among sterile male hybrids and the strong effect of individual loci, further exploration of the genes uncovered here may identify a universal mechanism for the evolution of hybrid sterility. </p>
Six genome assemblies of Drosophila species for: Identification and genetic analysis of a pervasive “needle-eye” sperm phenotype in Drosophila sterile hybrid males
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Data from: Genetic dissection of hybrid male sterility across stages of spermatogenesis
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Data from: Reproductive isolation caused by azoospermia in sterile male hybrids of Drosophila
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Data from: Genetic basis to hybrid inviability is more complex than hybrid male sterility in Caenorhabditis nematodes
Hybrid male sterility often evolves before female sterility or inviability of hybrids, implying that the accumulation of divergence between separated lineages should lead hybrid male sterility to have a more polygenic basis. However, experimental evidence is mixed. Here, we use the nematodes Caenorhabditis remanei and C. latens to characterize the underlying genetic basis of asymmetric hybrid male sterility and hybrid inviability. We demonstrate that hybrid male sterility is consistent with a simple genetic basis, involving a single X-autosome incompatibility. We also show that hybrid inviability involves more genomic compartments, involving diverse nuclear-nuclear incompatibilities, a mito-nuclear incompatibility, and maternal effects. These findings demonstrate that male sensitivity to genetic perturbation may be genetically simple compared to hybrid inviability in Caenorhabditis and motivates tests of generality for the genetic architecture of hybrid incompatibility across the breadth of phylogeny.
Data from: Genetics of hybrid male sterility among strains and species in the Drosophila pseudoobscura species group
Taxa in the early stages of speciation may bear intraspecific allelic variation at loci conferring barrier traits in hybrids such as hybrid sterility. Additionally, hybridization may spread alleles that confer barrier traits to other taxa. Historically, few studies examine within- and between-species variation at loci conferring reproductive isolation. Here, we test for allelic variation within Drosophila persimilis and within the Bogota subspecies of D. pseudoobscura at regions previously shown to contribute to hybrid male sterility. We also test whether D. persimilis and the USA subspecies of D. pseudoobscura share an allele conferring hybrid sterility in a D. pseudoobscura bogotana genetic background. All loci conferred similar hybrid sterility effects across all strains studied, though we detected some statistically significant quantitative effect variation among D. persimilis alleles of some hybrid incompatibility QTLs. We also detected allelism between D. persimilis and D. pseudoobscura USA at a 2nd chromosome hybrid sterility QTL. We hypothesize that either the QTL is ancestral in D. persimilis and D. pseudoobscura USA and lost in D. pseudoobscura bogotana, or gene flow transferred the QTL from D. persimilis to D. pseudoobscura USA. We discuss our findings in the context of population features that may contribute to variation in hybrid incompatibilities.
Data from: Genetic basis to hybrid inviability is more complex than hybrid male sterility in Caenorhabditis nematodes
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Data from: Genetics of hybrid male sterility among strains and species in the Drosophila pseudoobscura species group
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Data from: Misregulation of spermatogenesis genes in Drosophila hybrids is lineage-specific and driven by the combined effects of sterility and fast male regulatory divergence.
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Comparative transcriptome analysis of Brassica napus L. male sterility induced by the Chemical Hybridization Agent Monosulfuron Ester Sodium
GEO Series GSE53468. Brassica napus. 24 samples. Type: Expression profiling by array.
Data from: Interspecific Y chromosome variation is sufficient to rescue hybrid male sterility and is influenced by the grandparental origin of the chromosomes
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Fine scale genetic mapping of a Hybrid Male Sterility factor between Drosophila simulans and D. mauritiana
GEO Series GSE25339. Drosophila melanogaster. 10 samples. Type: Expression profiling by array.
The underlying pathway involved in inter-subspecific hybrid male sterility in rice
GEO Series GSE110483. Oryza sativa. 9 samples. Type: Expression profiling by high throughput sequencing.
Analysis of sex chromosomes effect on hybrid male sterility
GEO Series GSE4677. Drosophila melanogaster; Drosophila sechellia; Drosophila simulans; Drosophila sechellia x Drosophila simulans. 12 samples. Type: Expression profiling by array.
RNase ZS1-mediated UbL40 mRNA decay controls thermo-sensitive genic male sterility in hybrid rice
GEO Series GSE42467. Oryza sativa Japonica Group. 2 samples. Type: Expression profiling by high throughput sequencing.
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