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42 results for “immunohistochemistry”
Immunohistochemistry of Multimodal profiling of lung granulomas in macaques reveals cellular correlates of tuberculosis control
<p>(A) Architecture of macaque TB lung granuloma, where lymphocytes and macrophages are present in distinct regions. Immunohistochemistry and confocal microscopy were performed on a granuloma from an animal at 11 weeks post-Mtb infection to visualize localization of CD11c+ macrophages (cyan), CD3+ T cells (yellow), and CD20+ B cells (magenta)</p> <p>(B) Detection of mast cells in a 10-week NHP granuloma using immunohistochemistry, staining for tryptase (green) and c-kit (CD117)(red).</p> <p>(C) Detection of mast cells in a human lung granuloma. Hematoxylin and eosin stain and immunohistochemistry with multinucleated giant cells (stars, (top left) and c-kit (CD117) staining (indicated by arrows, top and bottom right).</p>
Deep learning chronic wasting disease (CWD) immunohistochemistry (IHC) image dataset
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Expression of CDCA2 in hepatocellular carcinoma by immunohistochemistry and patients' information
<p>Cohort of hepatocellular carcinoma patients enrolled in the study entitled "Cell division cycle associated 2 (CDCA2) upregulation promotes the progression of hepatocellular carcinoma in a p53-dependant mannar". CDCA2 expression in paraffin-embedded liver cancer tissue sections was evaluated by immunohistochemistry. Patients' clinicopathological information was collected.</p>
Immunohistochemistry of wild type and hjv-/- iron manipulated mouse livers and spleens treated with LPS or Hepcidin
<p><span>The iron hormone hepcidin is transcriptionally activated by iron or inflammation via distinct, partially overlapping pathways. We addressed how iron affects inflammatory hepcidin levels and the ensuing hypoferremic response. Dietary iron overload did not mitigate hepcidin induction in LPS-treated wt mice but prevented effective inflammatory hypoferremia. Likewise, LPS modestly decreased serum iron in hepcidin-deficient Hjv-/- mice, model of hemochromatosis. Synthetic hepcidin triggered hypoferremia in control but not iron-loaded wt animals. Furthermore, it dramatically decreased hepatic and splenic ferroportin in Hjv-/- mice on standard or iron-deficient diet, but only triggered hypoferremia in the latter. Mechanistically, iron antagonized hepcidin responsiveness by inactivating IRPs in the liver and spleen, to stimulate ferroportin mRNA translation. Prolonged LPS treatment eliminating ferroportin mRNA permitted hepcidin-mediated hypoferremia in iron-loaded mice. Thus, de novo ferroportin synthesis is critical determinant of serum iron and finetunes hepcidin-dependent functional outcomes. Our data uncover a crosstalk between hepcidin and IRE/IRP systems that controls tissue ferroportin expression and determines serum iron levels. Moreover, they suggest that hepcidin supplementation therapy is more efficient combined with iron depletion.</span></p>
Immunohistochemistry of wild type and hjv-/- iron manipulated mouse livers and spleens treated with LPS or Hepcidin
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Differences in local immune cell landscape between Q fever and atherosclerotic abdominal aortic aneurysms identified by multiplex immunohistochemistry
<p><strong>Background:</strong> Chronic Q fever is a zoonosis caused by the bacterium <em>Coxiella burnetii</em> which can manifest as infection of an abdominal aortic aneurysm (AAA). Antibiotic therapy often fails, resulting in severe morbidity and high mortality. Whereas previous studies have focused on inflammatory processes in blood, the aim of this study was to investigate local inflammation in aortic tissue.</p> <p><strong>Methods:</strong> Multiplex immunohistochemistry was used to investigate local inflammation in Q fever AAAs compared to atherosclerotic AAAs in aorta tissue specimen. Two six-plex panels were used to study both the innate and adaptive immune system.</p> <p><strong>Results: </strong>Q fever AAAs and atherosclerotic AAAs contained similar numbers of CD68<sup>+</sup> macrophages and CD3<sup>+</sup> T cells. However, in Q fever AAAs the number of CD68<sup>+</sup>CD206<sup>+</sup> M2 macrophages was increased, while expression of GM-CSF was decreased compared to atherosclerotic AAAs. Furthermore, Q fever AAAs showed an increase in both the number of CD8<sup>+</sup> cytotoxic T cells and CD3<sup>+</sup>CD8<sup>-</sup>FoxP3<sup>+</sup> regulatory T cells. Lastly, Q fever AAAs did not contain any well-defined granulomas.</p> <p><strong>Conclusions:</strong> These findings demonstrate that despite the presence of pro-inflammatory effector cells, persistent local infection with <em>C. burnetii</em> is associated with an immune suppressed micro environment.</p> <p><strong>Funding:</strong> This work was supported by SCAN consortium: European Research Area - CardioVascualar Diseases (ERA-CVD) grant [JTC2017-044] and TTW-NWO open technology grant [STW-14716].</p>
Immunohistochemistry validation by comparison of two antibodies
<p><strong><span>Supplementary figure 1. Immunohistochemistry validation by comparison of two antibodies. </span></strong><span>The panels show immunostaining results obtained by two independent TFF1 antibodies. Using MSVA-482M, a distinct cytoplasmic staining was seen in surface epithelial cells (but not of glands) in the stomach (A), subsets of goblet cells in the duodenum (B) and the colon (C), a subset of mucinous cells in the submandibulary gland (D), a subset of luminal epithelial cells and intraluminal mucus in the breast (E), a small subset of urothelial cells (mostly umbrella cells) in the renal pelvis (F), a large subset of urothelial cells in an inflamed urinary bladder (G), and in epithelial cells of the gallbladder (H). Using clone EPR3972, a comparable staining was seen in the stomach (a), the duodenum (b), the colon (c), the submandibulary gland (d), the breast (e), the renal pelvis (f), the bladder (g), and the gallbladder (h). The images A-H and a-h are from consecutive tissue sections.</span></p>
Ancillary immunohistochemistry testing for loss of p16 in melanoma
<p>This is the data set and R code used in a systematic review and meta-analysis on the accuracy of p16 loss by immunohistochemistry for melanoma diagnosis obtained from 26 studies involving 979 melanomas and 974 nevi.</p>
Fig. 6 in Hormonal Regulation of Testicular Development in the Finless Porpoise Neophocaena asiaeorientalis sunameri: Preliminary Evidence from Testicular Histology and Immunohistochemistry
Fig. 6. Immuno-expression of AR (A:3.5yr.) and ERβ (B: 0.1yr., C: 3.5yr. and D: 8yr.) in testes. Scale bar = 50 µm.
Figure 2 shows a in Hormonal Regulation of Testicular Development in the Finless Porpoise Neophocaena asiaeorientalis sunameri: Preliminary Evidence from Testicular Histology and Immunohistochemistry
Figure 2 shows a longitudinal slice of a tooth consisting of translucent and opaque zones under the transmitted light. The tooth is clearly divided into two parts by a highlighted neonatal line. The part inside of the neonatal line is dentine, and the outside is cementum. Ages were counted by GLGs in dentine, and basic data are shown in table 1. The oldest individual was 13 years old, while the youngest ones died about a month after birth.
Fig. 3 in Hormonal Regulation of Testicular Development in the Finless Porpoise Neophocaena asiaeorientalis sunameri: Preliminary Evidence from Testicular Histology and Immunohistochemistry
Fig. 3. Histological sections of testes from birth to adulthood. LC: Leydig cells; SC: Sertoli cells; PG: Primordial germ cells; SG: Spermatogonia; PS: Primary spermatocytes; SS: Secondary spermatocytes; SPZ: Spermatozoa. (A) 0.1yr. (B) 2.5yr. (C) 3yr. (D) 3.5yr. (E) 4.5yr. (F) 6yr. (G) 8yr. (H) 13yr. Scale bar = 50 µm.
Fig. 2 in Hormonal Regulation of Testicular Development in the Finless Porpoise Neophocaena asiaeorientalis sunameri: Preliminary Evidence from Testicular Histology and Immunohistochemistry
Fig. 2. Growth layer groups (GLGs) in the thin section of a tooth. One GLG consists of an opaque layer and a translucent layer. The arrow represents the neonatal line. Scale bar = 200 µm.
Fig. 5 in Hormonal Regulation of Testicular Development in the Finless Porpoise Neophocaena asiaeorientalis sunameri: Preliminary Evidence from Testicular Histology and Immunohistochemistry
Fig. 5. Immunoexpression of testosterone (A: 0.1yr, B: 3.5yr and C: 8yr) and estradiol (D: 0.1yr, E: 3.5yr and F: 8yr) in testes from birth to adulthood. Upper insert on panel A: negative control. Black arrows represent spermatozoa. Scale bar = 50 µm. © 2018 Academia Sinica, Taiwan
Fig. 4 in Hormonal Regulation of Testicular Development in the Finless Porpoise Neophocaena asiaeorientalis sunameri: Preliminary Evidence from Testicular Histology and Immunohistochemistry
Fig. 4. Scatter plots of TM against age as well as body length (A, B); STD against age as well as body length (C, D); TTA against age as well as body length (E, F). © 2018 Academia Sinica, Taiwan
Assessing Tumor-Infiltrating Lymphocytes in Breast Cancer: A Proposal for Combining Immunohistochemistry and Gene Expression Analysis to Refine Scoring
<p>Whole tissue scans of histochemistry (H&E) and immunohistochemistry (CD3, CD4, CD8 andCD45) images that have been used to calculate TIL scores.</p> <p>All stainings are numbers for each patient..</p>
A WT1-positive pleural neoplasm. Is it always a mesothelioma? Diagnostic pitfall of WT1 immunohistochemistry in pleural neoplasm
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Early Prediction of Oral Cancer by S100A7 Immunohistochemistry Signature-based Assessment
ClinicalTrials.gov study NCT04622462. IPD Sharing: UNDECIDED. Countries: 1. Publications: 6.
Comparative Effects of Metformin and Insulin on Stereological Studies and Immunohistochemistry of Placenta
ClinicalTrials.gov study NCT04907708. IPD Sharing: NO. Countries: 1. Publications: 1.
Cell Block Immunohistochemistry in Effusion Cytology
ClinicalTrials.gov study NCT04279327. IPD Sharing: NO. Countries: 1. Publications: 1.
Immunohistochemistry method for measuring autophagy flux using MAP1LC3/LC3 and SQSTM1 as core markers
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.