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213 results for “in vitro cultures”
Plant regeneration in leaf culture of Centaurium erythraea Rafn. Part 3: de novo transcriptome assembly and validation of housekeeping genes for studies of in vitro morphogenesis
<p>Six centaury transcriptomes (embryogenic calli, globular somatic embryos, cotyledonary somatic embryos, adventitious buds, leaves and roots of <em>in vitro</em> grown plants) were sequenced and <em>de novo</em> assembled using <a href="https://github.com/trinityrnaseq/trinityrnaseq/wiki">Trinity</a> .</p> <p><a href="https://zenodo.org/api/files/a0546879-e382-4cf9-8185-f188d1a0c5f0/CE_Assembly.tar.gz">CE_Assembly.tar.gz</a> - Centaury referent transcriptome comprises of 160.839 Trinity transcripts grouped in 105.726 Trinity genes.</p> <p><a href="https://zenodo.org/api/files/a0546879-e382-4cf9-8185-f188d1a0c5f0/CE_Assembly_fpkm.tar.gz">CE_Assembly_fpkm.tar.gz</a> - fpkm normalized read counts of the assembled transcripts in the six sequenced centaury tissues.</p> <p><a href="https://zenodo.org/api/files/a0546879-e382-4cf9-8185-f188d1a0c5f0/nt.db_CE_assembly.tar.gz">nt.db_CE_assembly.tar.gz</a> - annotation of assembled transcripts by mapping them against NCBI nucleotide (NT) database using BLASTn . The obtained results were filtered with E-value E ≤ 10<sup>-3</sup>.</p> <p><a href="https://zenodo.org/api/files/a0546879-e382-4cf9-8185-f188d1a0c5f0/swissprot.db_CE_assembly.tar.gz">swissprot.db_CE_assembly.tar.gz</a> - annotation of assembled transcripts by mapping them against NCBI nucleotide (<a href="https://zenodo.org/api/files/a0546879-e382-4cf9-8185-f188d1a0c5f0/swissprot.db_CE_assembly.tar.gz">s</a>wissprot) database using BLASTx . The obtained results were filtered with E-value E ≤ 10<sup>-3</sup>.</p> <p><a href="https://zenodo.org/api/files/a0546879-e382-4cf9-8185-f188d1a0c5f0/pfam30.db_CE_assembly.tar.gz">pfam30.db_CE_assembly.tar.gz</a> - annotation of assembled transcripts by mapping them against Pfam30 domain database using hmmer3. The obtained results were filtered with independent E-value E ≤ 10<sup>-3</sup>.</p>
Figure 2 in Enhancing survival of Demodex folliculorum (Acari: Demodecidae) under in vitro condition: Effect of temperature and culture media
Figure 2. Microphotograph (40×) of Demodex folliculorum mites in the hair follicle of six individuals with a clinical diagnosis of blepharitis. Note the different life stages of the mites (a = adult; l = larva; n = nymph; o = ovum).
Figure 1 in Enhancing survival of Demodex folliculorum (Acari: Demodecidae) under in vitro condition: Effect of temperature and culture media
Figure 1. Photograph of the eyelids of a patient with cylindrical dandruff attached to the eyelid margin and around the eyelash base.
Figure 4 in Dactylorhiza Fuchsii (Druce) Soó As A Model Object In In Vitro Culture Study For Development Of Terrestrial Orchids
Figure 4. Root of D. fuchsii growing in the Figure 5. Root of D. fuchsii one year after exposition in NBG: arrow indicates elaborate transplanting ex vitro: arrow indicates some coiled structures known as pelotons of peloton-like structure as they do within the orchid mycorrhizal fungi in the root cortex (section was cells (section was stained with Trypan Blue; bar stained with Trypan Blue; bar = 50 mm) = 50 mm).
Figure 1 in Dactylorhiza Fuchsii (Druce) Soó As A Model Object In In Vitro Culture Study For Development Of Terrestrial Orchids
Figure 1. Development of Dactylorhiza fuchsii (Druce) Soó in vitro conditions: A – beginning of morphogenesis after germination with development of protocorms, characteristic for orchids; B – initialization of rooting; C – sterile plantlets after exposition at 2 °C in the dark, simulating the natural dormancy period; D – plantlet before transplanting ex vitro (ro – root; tu – tuber).
Fig. S1. Toad skin swab cultures. Culture plates from A in Comparison of in vitro methods to inhibit growth of a virulent strain of Batrachochytrium dendrobatidis (Longcore, Pessier, and Nichols 1999)
Fig. S1. Toad skin swab cultures. Culture plates from A. boreas skin swabs after three days incubation at 25 °C.
Fig. 3 in Peculiarities Of Embryonic And Post-Embryonic Development Of Oesophagostomum Dentatum (Nematoda, Strongylidae) Larvae Cultured In Vitro
Fig. 3. Formation of Оesophagostomum dentatum L3 in vitro: a (×100); b (×400).
Fig. 2 in Peculiarities Of Embryonic And Post-Embryonic Development Of Oesophagostomum Dentatum (Nematoda, Strongylidae) Larvae Cultured In Vitro
Fig. 2. Post-embryonic development of Оeso-
Fig. 1 in Peculiarities Of Embryonic And Post-Embryonic Development Of Oesophagostomum Dentatum (Nematoda, Strongylidae) Larvae Cultured In Vitro
Fig. 1. Embryonic development of Оesophagostomum dentatum in vitro: а — fragmentation of
Remotely controlled 3D-engineered scaffolds for biomimetic in vitro investigations on brain cell co-cultures
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Image 1 in Associated callus culture technique for in vitro growth of rust fungi
Image 1. Malformations induced
Raw data for the article "In vitro culture of leukemic cells in collagen scaffolds and carboxymethylcellulose-polyethylene glycol gel"
<p>Uncropped original micrographs and raw numerical data of figures and tables from the article submitted to PeerJ. </p>
Fig. 2 in Comparative transcriptome analysis infers bulb derived in vitro cultures as a promising source for sipeimine biosynthesis in Fritillaria cirrhosa D. Don (Liliaceae, syn. Fritillaria roylei Hook.) - High value Himalayan medicinal herb
Fig. 2. (A–E) Differential gene expression analysis in comparative F. roylei transcriptome: (A) Heat-map showing differential gene expression in the bulb (PKW) vs callus (PK2); bulb (PKW) vs in vitro regenerated plantlets (PK1) and callus (PK2) vs in vitro regenerated plantlets (PK1); (B) Venn diagram represents the differential gene expression in PKW vs PK1; PKW vs PK2; PK2 vs PK1, (C–E) Volcano plots represents the differential gene expression in PKW vs PK1; PKW vs PK2; PK2 vs PK1 as colour description image, where p-value & log2 fold-change in red colour represents genes with log2 fold-change cut off 2 and p-value <=0.05; p-value in blue colour represents genes with no cut off on log2 fold-change and p-value <=0.05. Whereas, log2 fold-change in green colour represents genes with fold-change cut off 2 but no p-value cut off and non-significant (NS) in grey colour represents genes with no filter on log2 fold-change and p-value, respectively. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Fig. 1 in Comparative transcriptome analysis infers bulb derived in vitro cultures as a promising source for sipeimine biosynthesis in Fritillaria cirrhosa D. Don (Liliaceae, syn. Fritillaria roylei Hook.) - High value Himalayan medicinal herb
Fig. 1. (A–F) Functional annotations and unigenes classification of comparative F. roylei transcriptome: (A) Unigenes annotation with top 15 different plant species; (B) Top 5 pathway representation as per Kyoto Encyclopedia of Genes and Genomes; (C) Gene Ontology classification under the cellular component, molecular function, and biological process categories; (D) COG (Cluster of Orthologous Groups of proteins) classification into nine different categories; (E) Unigenes classification into major transcription factor families; (F) Gene family and sub-family classification using TAIR database.
Fig. 4 in Comparative transcriptome analysis infers bulb derived in vitro cultures as a promising source for sipeimine biosynthesis in Fritillaria cirrhosa D. Don (Liliaceae, syn. Fritillaria roylei Hook.) - High value Himalayan medicinal herb
Fig. 4. Comparative expression pattern validation for the sipeimine biosynthetic pathway genes as obtained from RNA-Seq data and qRT-PCR.
Fig. 3 in Comparative transcriptome analysis infers bulb derived in vitro cultures as a promising source for sipeimine biosynthesis in Fritillaria cirrhosa D. Don (Liliaceae, syn. Fritillaria roylei Hook.) - High value Himalayan medicinal herb
Fig. 3. Proposed sipeimine biosynthetic pathways in F. roylei. Heat-map showing gene expression in the bulb, callus, and regenerated plantlets. The genes were mapped using TPM (transcripts per kilobase million) values and colour-coded by increasing relative expression. The broken dotted arrow represents putative terminal biosynthesis steps. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)
Exosome-Enriched Culture Media to Enhance In Vitro Maturation and Embryo Development in Poor Ovarian Responders Undergoing IVF
ClinicalTrials.gov study NCT07085312. IPD Sharing: NO. Countries: 1. Publications: 2.
Optimizing the Temperature for Embryo Culture in In Vitro Fertilization
ClinicalTrials.gov study NCT01506089. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Adding Cytokines to In Vitro Human Culture Media to Improve Embryogenesis and Implantation
ClinicalTrials.gov study NCT02420886. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Comparative molecular analysis of cancer behavior cultured in vitro, in vivo, and ex vivo
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.