Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
84
datasets available to search
ShareScore release 0.9.0
Dataset results
84 results for “inbred lines”
Cellular and Humoral Immune Responses after Immunisation with Low Virulent African Swine Fever Virus in the Large White Inbred Babraham Line and Outbred Domestic Pigs
<p>Raw data for manuscript. Contains temperature, clinical scores, qPCR, blood cell numbers and immune responses over time for two groups of pigs immunised with low virulent African swine fever virus and challenged with highly virulent virus. Data for each panel or figure is displayed on a separate worksheet in the file. The readme worksheet contains a brief description of each figure. The majority of data is displayed in an XY table format, with the number of days post immunisation with low virulent virus indicated.</p>
Data from: Solanum pennellii (LA5240) backcross inbred lines (BILs) for high resolution mapping in tomato
<p>Wild species are an invaluable source of new traits for crop improvement. Over the years the tomato community bred cultivated lines that carry introgressions from different species of the tomato tribe to facilitate trait discovery and mapping. The next phase in such projects is to find the genes that drive the identified phenotypes. This can be achieved by genotyping a few thousand individuals resulting in fine-mapping that can potentially identify the causative gene. To couple trait discovery and fine mapping we are presenting large, recombination-rich, Backcross Inbred Line (BIL) populations involving an unexplored accession of the wild, green-fruited species Solanum pennellii (LA5240; the Lost Accession) with two modern tomato inbreds: LEA, determinate, and TOP indeterminate. The LEA and TOP BILs are in BC2F6-8 generation and include 1,400 and 500 lines respectively. The BILs were genotyped with ~5,000 SPET markers, showing that in the euchromatic regions there was one recombinant every 17-18 Kb while in the heterochromatin a recombinant every 600-700 Kb (TOP and LEA respectively). To gain perspective on the topography of recombination we compared five independent members of the self-pruning gene family with their respective neighboring genes; based on PCR markers, in all cases we found recombinants. Further mapping analysis of two known morphological mutations that segregated in the BILs (Self-pruning and Hair), showed that the maximal delimited intervals were 73 Kb and 210 Kb respectively and included the known causative genes. The LOST_BILs provide a solid framework to study traits derived from a tolerant wild tomato.</p>
Data from: Solanum pennellii (LA5240) backcross inbred lines (BILs) for high resolution mapping in tomato
Open the record for dataset details and reuse information.
SNP markers used for QTL mapping in the inbred lines
<p><span>Young leaves of the 175 inbred lines and their seven parents were collected from seedlings grown in a greenhouse. </span><span>About 200 mg bulk leaf sample from three plants of a line was placed in 2 ml safe-lock </span><span>Eppendorf tube and stored at ‒80 </span><span>˚C for one night prior to crushing using a Mixer Mill (TissueLyser II, Qiagen, Germany). Genomic DNA was extracted using SIGMA DNA extraction kit (Sigma-Aldrich, St. Louis, MO, USA) following the manufacturer's instruction. DNA concentration and purity of the samples were assessed using a NanoDrop 2000c spectrophotometer (Thermo Scientific, Wilmington, DE, USA). The samples were processed and sequenced using tunable genotyping-by-sequencing (tGBS®) method by Data2Bio (Ames, IW, USA). Genomic DNA was digested using two restriction enzymes NSpI (5′-RCATG^Y-3′) and BfuCI/Sau3AI (5′-^GATC-3′) which created 3´and 5´overhangs, respectively. Two single-stranded oligos, one containing a sample-specific internal barcode and the other a universal oligo, were ligated to the complementary 3´ and 5´ overhangs, respectively. </span>All 175 inbred lines' and seven parents' treated DNA was pooled for construction of the tGBS library and sequencing. The raw sequence data were demultiplexed by barcode, which was subsequently removed bioinformatically from each sequence. The barcode-trimmed sequence reads of genotype were further trimmed using the trimming software, Lucy (Chou & Holmes, 2001; Li & Chou 2004) to remove low-quality reads based on Phred quality scores of Q15.</p>
SNP markers used for QTL mapping in the inbred lines
Open the record for dataset details and reuse information.
The 49,890 SNPgenotype derived from genotyping-by-sequencing strategy for the NIP/9311 backcross inbred lines population
<p>Transmission ratio distortion (TRD) refers to a widespread phenomenon in which one allele is transmitted by heterozygotes more frequently to the progeny than the opposite allele. TRD is considered as a mark suggesting the presence of reproductive barrier. However, the genetic and molecular mechanisms underlying TRD in rice remain largely unknown. In the present study, a population of backcross inbred lines (BILs) derived from the cross of a japonica cultivar Nipponbare and an indica variety 9311 was utilized to study the genetic base of TRD. A total of eighteen genomic regions were identified for TRD in the BILs. Among them, twelve and six regions showed indica (9311) and japonica (Nipponbare) alleles with preferential transmission, respectively. A series of F2 populations were used to confirm the TRD effects, including six genomic regions that were confirmed by chromosome segment substitution line (CSSL)-derived F2 populations from inter-subspecific allelic combinations. However, none of the regions was confirmed by the CSSL-derived populations from intra-subspecific allelic combination. Furthermore, significant epistatic interaction was found between TRD1.3 and TRD8.1 suggesting that TRD could positively contribute to breaking inter-subspecific reproductive barriers. Our results have laid the foundation for identifying the TRD genes and provide an effective strategy to breakdown TRD for breeding wide-compatible lines, which will be further utilized in the inter-subspecific hybrid breeding programs.</p>
Images of flowers and tubers produced by potato recombinant inbred lines
<p>Recombinant inbred line (RIL) populations are powerful mapping tools in many crops but have not yet been created using cultivated potato germplasm. We crossed the doubled monoploid cultivated clone DM 1-3 with the self-compatible diploid inbred wild clone M6 to create a diploid F1 hybrid. One F1 plant was self-pollinated to generate a phenotypically diverse F2 population, which was selfed to create 87 RILs. This is the first report of a RIL population developed from a cultivated x wild hybrid in potato. Poor fertility was a significant challenge in creating RILs. Nevertheless, we generated inbred lines that ranged from high to low fertility, vigor, and tuber production. F6 RILs ranged from 98% to 68% homozygosity, based on 2884 SNP markers. Considering the phenotypic variability between the two parents and among the RILs, we expect the RIL population to be valuable for mapping traits important to the potato industry.</p>
Genetic Control of Reproductive Traits under Different Temperature Regimes in Inbred Line Populations Derived from Crosses between S. pimpinellifolium and S. lycopersicum Accessions
<p>Marker genotypes, linkage map and phenotypic data used for QTL analysis included in the manuscript by Gonzalo et al. <em>in press "</em>Genetic Control of Reproductive Traits under Different Temperature Regimes in Inbred Line Populations Derived from Crosses between <em>S. pimpinellifolium</em> and <em>S. lycopersicum</em> Accessions".</p>
Sorghum leaf blight phenotypes for two recombinant inbred line populations
<p>Sorghum leaf blight and northern corn leaf blight, both caused by <em>Exserohilum turcicum</em>, are major diseases of sorghum and maize, respectively. Examining the genetic architecture of resistance in sorghum will lead to a better understanding of the relationship between resistance in sorghum and maize, which can ultimately enhance management options in both crops. In 2018 and 2019 we evaluated two sorghum recombinant inbred line (RIL) populations for resistance to <em>E. turcicum</em>. The BTx623 x IS3620C and BTx623 x SC155 populations consisted of 235 and 81 RILs, respectively. Resistance in both populations was moderately to highly heritable. We identified a total of six quantitative trait loci (QTL) across the two populations. Three QTL with small to moderate effect sizes were identified in the BTx623 x IS3620C population. Three QTL, including a large-effect QTL on chromosome three that explained 24% of the variation, were identified in the BTx623 x SC155 population. We compared the identified QTL with the position of northern corn leaf blight candidate genes and found eight candidate resistance gene orthologs that colocalize with the sorghum leaf blight QTL. There were also several nucleotide-binding leucine rich repeat encoding genes within the candidate intervals. Understanding host resistance in multiple species furthers our understanding of the<em> Exserohilum turcicum</em> pathosystem.</p>
Datasets on Landraces and Inbred lines
<p>Datasets on Maize Landraces and Inbred lines used in Dromamed PRIMA project</p>
Sorghum leaf blight phenotypes for two recombinant inbred line populations
Open the record for dataset details and reuse information.
The 49,890 SNPgenotype derived from genotyping-by-sequencing strategy for the NIP/9311 backcross inbred lines population
Open the record for dataset details and reuse information.
Images of flowers and tubers produced by potato recombinant inbred lines
Open the record for dataset details and reuse information.
Data from: Sequence analysis of European maize inbred line F2 provides new insights into molecular and chromosomal characteristics of presence/absence variants
Maize is well known for its exceptional structural diversity, including copy number variants (CNVs) and presence/absence variants (PAVs), and there is growing evidence for the role of structural variation in maize adaptation. While PAVs have been described in this important crop species, the extent of presence/absence variation and the relative position of inbred-specific regions remain to be elucidated. De novo genome sequencing of the F2 maize inbred line which played a key role in European breeding programs over the past 50 years revealed thousands of novel genomic regions, making up 88Mb of DNA, that are present in the F2 but not in B73. Comparison of B73 and F2 PAV localization revealed contrasted chromosomal distributions between the two inbreds and specific evolutionary dynamics of PAVs as compared to SNPs. Detailed sequence and functional annotation of F2 PAV sequences revealed hundreds of new genes with transcriptional support, but also a large fraction of repetitive sequences. Detailed analysis of sequence breakpoint highlights the role of double strand break repair, but also transposon insertion in PAV generation. Typing of the B73 and F2 PAVs in maize temperate inbreds revealed that some PAVs are found only in European Flint material, thus pinpointing structural features that may be at the origin of adaptive traits involved in the success of this material. Linkage disequilibrium (LD) analysis revealed that LD is strong within PAVs, as expected by the absence of recombination in crosses where PAV is missing in one of the parents.
Data from: Draft assembly of elite inbred line PH207 provides insights into genomic and transcriptome diversity in maize
Intense artificial selection over the last 100 years has produced elite maize (Zea mays) inbred lines that combine to produce high-yielding hybrids. To further our understanding of how genome and transcriptome variation contribute to the production of high-yielding hybrids, we generated a draft genome assembly of the inbred line PH207 to complement and compare with the existing B73 reference sequence. B73 is a founder of the Stiff Stalk germplasm pool, while PH207 is a founder of Iodent germplasm, both of which have contributed substantially to the production of temperate commercial maize and are combined to make heterotic hybrids. Comparison of these two assemblies revealed over 2,500 genes present in only one of the two genotypes and 136 gene families that have undergone extensive expansion or contraction. Transcriptome profiling revealed extensive expression variation, with as many as 10,564 differentially expressed transcripts and 7,128 transcripts expressed in only one of the two genotypes in a single tissue. Genotype-specific genes were more likely to have tissue/condition-specific expression and lower transcript abundance. The availability of a high-quality genome assembly for the elite maize inbred PH207 expands our knowledge of the breadth of natural genome and transcriptome variation in elite maize inbred lines across heterotic pools.
Variation in leaf transcriptome responses to elevated ozone corresponds with physiological sensitivity to ozone across maize inbred lines
<p>All FASTA files used for BLAST analyses and all BLAST results for <a href="https://doi.org/10.1093/genetics/iyac080">https://doi.org/10.1093/genetics/iyac080</a> are included. Descriptions of each file can be found in README_maize_genetics_2022_zenodo.csv and further information about the content of the files can be found on <a href="https://github.com/McIntyre-Lab/papers/tree/master/nanni_maize_2022">github</a>.</p>
Genotype by sequencing data from CHPRRU2, a panel of 282 maize inbred lines.
<p>Genotype by sequencing data from CHPRRU2, a panel of 282 maize inbred lines.</p>
Data from: Draft assembly of elite inbred line PH207 provides insights into genomic and transcriptome diversity in maize
Open the record for dataset details and reuse information.
Data from: Sequence analysis of European maize inbred line F2 provides new insights into molecular and chromosomal characteristics of presence/absence variants
Open the record for dataset details and reuse information.
The severity of infectious bursal disease in White Leghorn inbred chicken lines is associated with greater bursal inflammation in vivo and more rapid induction of pro-inflammatory responses following
GEO Series GSE166026. Gallus gallus. 26 samples. Type: Expression profiling by high throughput sequencing.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.